Reviewed, UniProtKB/Swiss-Prot Q71ZD6 (LDH2_LISMF)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase 2
      Short name=L-LDH 2
    EC=1.1.1.27

Gene names

Name:	ldh2
Ordered Locus Names:	LMOf2365_1553

Organism

Listeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]

Taxonomic identifier

265669 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD(+) = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	L-lactate dehydrogenase 2		PRO_0000168364
Regions
	Nucleotide binding	12 – 40	29	NAD By similarity
Sites
	Active site	177	1	Proton acceptor
	Binding site	90	1	Substrate By similarity
	Binding site	122	1	NAD or substrate By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	230	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q71ZD6-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 1B288BDBDC01DC07	FASTA	311	33,783
	10 20 30 40 50 60 MKPRKVMIIG AGNVGTAAAH AFVNQKFVEE LILVDLNKER VEGNRKDLAD AAAFMPGKMD 70 80 90 100 110 120 ITVRDASDCA DVDIAVITVT AGPLKEGQTR LDELRSTSRI VSSIVPEMMK GGFNGIFLIA 130 140 150 160 170 180 TNPCDIITYQ VWKLSGLPRE RVLGTGVWLD TTRLRRLLAE KLDIAAQSID AFILGEHGDS 190 200 210 220 230 240 QFPVWSHSSI YGKPVNEYSV EKLGEALDLK QIGETARDTG FEIYHQKGCT EYGIGGTIVE 250 260 270 280 290 300 ICRHIFSGSQ RALTVSCVLD GEYGESGLAI GVPAVLSQNG VKEIISLKLD EKEKEAFANS 310 VAVIKKSIQS I

References

[1]

"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M. Nucleic Acids Res. 32:2386-2395(2004) [PubMed: 15115801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017262 Genomic DNA. Translation: AAT04328.1.
RefSeq	YP_014151.1.
3D structure databases
HSSP	HSSP built from PDB template 1A5Z based on UniProtKB P16115.
ModBase	Search...
Genome annotation databases
GeneID	2799266.
GenomeReviews	Gene locus LMOf2365_1553 in contig AE017262_GR.
KEGG	lmf:LMOf2365_1553.
TIGR	LMOf2365_1553.
Phylogenomic databases
HOGENOM	Q71ZD6.
Enzyme and pathway databases
BioCyc	LMON265669:LMOF2365_1553-MON.
Family and domain databases
HAMAP	MF_00488. [Tree]
InterPro	IPR001557. L-lactate/malate_DHase. IPR011304. L-lactate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH2_LISMF

Accession

Primary (citable) accession number: Q71ZD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)