Reviewed, UniProtKB/Swiss-Prot Q71Z77 (ARGJ_LISMF)

Names and origin

Protein names

Recommended name:
    Arginine biosynthesis bifunctional protein argJ
Cleaved into the following 2 chains:
    1- Recommended name:
            Arginine biosynthesis bifunctional protein argJ alpha chain
    2- Recommended name:
            Arginine biosynthesis bifunctional protein argJ beta chain
Including the following 2 domains:
    1- Recommended name:
            Glutamate N-acetyltransferase
              EC=2.3.1.35
        Alternative name(s):
            Ornithine acetyltransferase
              Short name=OATase
            Ornithine transacetylase
    2- Recommended name:
            Amino-acid acetyltransferase
              EC=2.3.1.1
        Alternative name(s):
            N-acetylglutamate synthase
              Short name=AGS

Gene names

Name:	argJ
Ordered Locus Names:	LMOf2365_1612

Organism

Listeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]

Taxonomic identifier

265669 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate By similarity. HAMAP MF_01106
Catalytic activity	N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate. HAMAP MF_01106 Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01106
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1. HAMAP MF_01106 Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01106
Subunit structure	Heterotetramer of two alpha and two beta chains By similarity. HAMAP MF_01106
Subcellular location	Cytoplasm Probable HAMAP MF_01106.
Miscellaneous	Some bacteria possess a monofunctional argJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway. HAMAP MF_01106
Sequence similarities	Belongs to the argJ family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 184	184	Arginine biosynthesis bifunctional protein argJ alpha chain By similarity		PRO_0000002185
	Chain	185 – 398	214	Arginine biosynthesis bifunctional protein argJ beta chain By similarity		PRO_0000002186
Sites
	Site	184 – 185	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q71Z77-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: A76F66951AEDCD31	FASTA	398	42,785
	10 20 30 40 50 60 MELVKGNIAS PKGFYADGKH AGLKRKRNDI GWIYSEVPAN AAAVYTMNQM QAAPIFVTKD 70 80 90 100 110 120 SFQSNAKLQA IIVNSGNANA CTGNQGMLDA LAMRAQTAEK LDIPLDYVAV ASTGIIGDML 130 140 150 160 170 180 PMDKINAGIE MLEKQTGNAA DFEEAILTTD TFQKQISFQT EIGGRKVTMS GVAKGSGMIH 190 200 210 220 230 240 PNMATMLAFI TTDAAIPAEL LQKLLKIKVD KTFNQITVDG DTSTNDMVVV MANGCAENPM 250 260 270 280 290 300 LQEGTADFAK FADMFQAVME HLAKSIARDG EGATKLIEVQ VNGATKTEDA RMIAKKIVSS 310 320 330 340 350 360 SLVKTAAFGG DGNWGRIICA IGYSGGRFAP DNITIKIGGI EILNHSSQTI FNQQALDAYL 370 380 390 EEEHIIIDVD LHIGLESGTA WGCDLSYEYV KINACYRT

References

[1]

"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M. Nucleic Acids Res. 32:2386-2395(2004) [PubMed: 15115801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017262 Genomic DNA. Translation: AAT04387.1.
RefSeq	YP_014210.1.
3D structure databases
SMR	Q71Z77. Positions 1-184, 10-394, 185-396.
ModBase	Search...
Protein-protein interaction databases
STRING	Q71Z77.
Genome annotation databases
GeneID	2799016.
GenomeReviews	Gene locus LMOf2365_1612 in contig AE017262_GR.
KEGG	lmf:LMOf2365_1612.
TIGR	LMOf2365_1612.
Phylogenomic databases
eggNOG	COG1364.
HOGENOM	HBG284202.
OMA	QNRFCAA.
Enzyme and pathway databases
BioCyc	LMON265669:LMOF2365_1612-MONOMER.
Family and domain databases
HAMAP	MF_01106. ArgJ. [Tree]
InterPro	IPR002813. Arg_biosynth_ArgJ. IPR016117. Pept_S58_DmpA/Arg_biosyn_ArgJ. [Graphical view]
PANTHER	PTHR23100. ArgJ. 1 hit.
Pfam	PF01960. ArgJ. 1 hit. [Graphical view]
TIGRFAMs	TIGR00120. ArgJ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGJ_LISMF

Accession

Primary (citable) accession number: Q71Z77

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)