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Q71Z07 (SYL_LISMF) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:LMOf2365_1684
OrganismListeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]
Taxonomic identifier265669 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

Protein attributes

Sequence length803 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 803803Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152038

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif575 – 5795"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5781ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q71Z07 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 0CBA3CA59982668F

FASTA80391,939
        10         20         30         40         50         60 
MTFNHKKMEP KWQQYWSEHN TFKTTEDKNK DNFYALDMFP YPSGAGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
LSRMKRMQGK NVLHPIGWDA FGLPAEQYAI DTGNDPEEFT ALNIANFTRQ IKSLGFSYDW 

       130        140        150        160        170        180 
DREINTTDPE YYKWTQWIFE KLYEKGLAYE AEIAVNWCPA LGTVLANEEV IDGKSERGGF 

       190        200        210        220        230        240 
PVFRKPMRQW MLKITAYADR LLDDLDLVDW PENIKDMQRN WIGRSEGAEV TFKIKDSDET 

       250        260        270        280        290        300 
FNVFTTRPDT LFGATYTVFA PEHELIEKIT TPEQKEAVEA YKKQVELKSE LERTDLAKDK 

       310        320        330        340        350        360 
TGVFTGAYAI NPINGEEVPI WIADYVLIQY GTGAIMAVPA HDERDFEFAQ QFGLNIRPVL 

       370        380        390        400        410        420 
EGGDVTKEAF TGDGPHINSD FLNGLAKAEA ITAAIDWLEK EGIGSRKITY RLRDWLFSRQ 

       430        440        450        460        470        480 
RYWGEPIPVI HWEDGETTLV PEDELPLLLP KATEIKPSGT GESPLANLHD WVNVTDKNGR 

       490        500        510        520        530        540 
KGRRETNTMP QWAGSSWYFL RYIDPNNSEA IADKEKLAEW LPVDVYIGGA EHAVLHLLYA 

       550        560        570        580        590        600 
RFWHKFLYDI GVVPTKEPFQ KLFNQGMILG ENNEKMSKSR GNVVNPDEVV EKYGADTLRL 

       610        620        630        640        650        660 
YEMFMGPLEA SIAWNENGLE GARKFLDRIW RLLVTEEGTL AEKVTTDANA NLEKAYHHMV 

       670        680        690        700        710        720 
KTVTNHYENL RFNTGISQLM IFINEAYKQD TIPKQYVEGF VQLLSPIAPH LAEELWEILG 

       730        740        750        760        770        780 
HTETISYVAW PTYDETKLVE DEVEIVLQVN GKVKSKITVA KSLEKEELEK IAQEDNKMKE 

       790        800 
NLEGKTIRKV IVVPGKLVNI VAN 

« Hide

References

[1]"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species."
Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. expand/collapse author list , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M.
Nucleic Acids Res. 32:2386-2395(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: F2365.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017262 Genomic DNA. Translation: AAT04457.1.
RefSeqYP_014280.1. NC_002973.6.

3D structure databases

ProteinModelPortalQ71Z07.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING265669.LMOf2365_1684.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT04457; AAT04457; LMOf2365_1684.
GeneID2798737.
KEGGlmf:LMOf2365_1684.
PATRIC20324603. VBILisMon105049_1689.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LISMF
AccessionPrimary (citable) accession number: Q71Z07
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries