Q71Z07 (SYL_LISMF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase
EC=6.1.1.4

Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS

Gene names

Name:	leuS
Ordered Locus Names:	LMOf2365_1684

Organism

Listeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]

Taxonomic identifier

265669 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Listeriaceae › Listeria › Listeria monocytogenes

Protein attributes

Sequence length	803 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 803	803	Leucine--tRNA ligase HAMAP-Rule MF_00049		PRO_0000152038
Regions
	Motif	40 – 51	12	"HIGH" region HAMAP-Rule MF_00049
	Motif	575 – 579	5	"KMSKS" region HAMAP-Rule MF_00049
Sites
	Binding site	578	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q71Z07 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 0CBA3CA59982668F	FASTA	803	91,939
	10 20 30 40 50 60 MTFNHKKMEP KWQQYWSEHN TFKTTEDKNK DNFYALDMFP YPSGAGLHVG HPEGYTATDI 70 80 90 100 110 120 LSRMKRMQGK NVLHPIGWDA FGLPAEQYAI DTGNDPEEFT ALNIANFTRQ IKSLGFSYDW 130 140 150 160 170 180 DREINTTDPE YYKWTQWIFE KLYEKGLAYE AEIAVNWCPA LGTVLANEEV IDGKSERGGF 190 200 210 220 230 240 PVFRKPMRQW MLKITAYADR LLDDLDLVDW PENIKDMQRN WIGRSEGAEV TFKIKDSDET 250 260 270 280 290 300 FNVFTTRPDT LFGATYTVFA PEHELIEKIT TPEQKEAVEA YKKQVELKSE LERTDLAKDK 310 320 330 340 350 360 TGVFTGAYAI NPINGEEVPI WIADYVLIQY GTGAIMAVPA HDERDFEFAQ QFGLNIRPVL 370 380 390 400 410 420 EGGDVTKEAF TGDGPHINSD FLNGLAKAEA ITAAIDWLEK EGIGSRKITY RLRDWLFSRQ 430 440 450 460 470 480 RYWGEPIPVI HWEDGETTLV PEDELPLLLP KATEIKPSGT GESPLANLHD WVNVTDKNGR 490 500 510 520 530 540 KGRRETNTMP QWAGSSWYFL RYIDPNNSEA IADKEKLAEW LPVDVYIGGA EHAVLHLLYA 550 560 570 580 590 600 RFWHKFLYDI GVVPTKEPFQ KLFNQGMILG ENNEKMSKSR GNVVNPDEVV EKYGADTLRL 610 620 630 640 650 660 YEMFMGPLEA SIAWNENGLE GARKFLDRIW RLLVTEEGTL AEKVTTDANA NLEKAYHHMV 670 680 690 700 710 720 KTVTNHYENL RFNTGISQLM IFINEAYKQD TIPKQYVEGF VQLLSPIAPH LAEELWEILG 730 740 750 760 770 780 HTETISYVAW PTYDETKLVE DEVEIVLQVN GKVKSKITVA KSLEKEELEK IAQEDNKMKE 790 800 NLEGKTIRKV IVVPGKLVNI VAN

References

[1]

"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M. Nucleic Acids Res. 32:2386-2395(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F2365.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017262 Genomic DNA. Translation: AAT04457.1.
RefSeq	YP_014280.1. NC_002973.6.
3D structure databases
ProteinModelPortal	Q71Z07.
ModBase	Search...
Protein-protein interaction databases
STRING	265669.LMOf2365_1684.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAT04457; AAT04457; LMOf2365_1684.
GeneID	2798737.
KEGG	lmf:LMOf2365_1684.
PATRIC	20324603. VBILisMon105049_1689.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0495.
HOGENOM	HOG000200748.
KO	K01869.
OMA	WIFLQIF.
ProtClustDB	PRK00390.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
HAMAP	MF_00049_B. Leu_tRNA_synth_B.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-ligase_bac/mito. IPR025709. Leu_tRNA-synth_edit. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF13603. tRNA-synt_1_2. 1 hit. PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYL_LISMF

Accession

Primary (citable) accession number: Q71Z07

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program