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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Listeria monocytogenes serotype 4b (strain F2365)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei578 – 5781ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:LMOf2365_1684
OrganismiListeria monocytogenes serotype 4b (strain F2365)
Taxonomic identifieri265669 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000563 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 803803Leucine--tRNA ligasePRO_0000152038Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi265669.LMOf2365_1684.

Structurei

3D structure databases

ProteinModelPortaliQ71Z07.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi575 – 5795"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q71Z07-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFNHKKMEP KWQQYWSEHN TFKTTEDKNK DNFYALDMFP YPSGAGLHVG
60 70 80 90 100
HPEGYTATDI LSRMKRMQGK NVLHPIGWDA FGLPAEQYAI DTGNDPEEFT
110 120 130 140 150
ALNIANFTRQ IKSLGFSYDW DREINTTDPE YYKWTQWIFE KLYEKGLAYE
160 170 180 190 200
AEIAVNWCPA LGTVLANEEV IDGKSERGGF PVFRKPMRQW MLKITAYADR
210 220 230 240 250
LLDDLDLVDW PENIKDMQRN WIGRSEGAEV TFKIKDSDET FNVFTTRPDT
260 270 280 290 300
LFGATYTVFA PEHELIEKIT TPEQKEAVEA YKKQVELKSE LERTDLAKDK
310 320 330 340 350
TGVFTGAYAI NPINGEEVPI WIADYVLIQY GTGAIMAVPA HDERDFEFAQ
360 370 380 390 400
QFGLNIRPVL EGGDVTKEAF TGDGPHINSD FLNGLAKAEA ITAAIDWLEK
410 420 430 440 450
EGIGSRKITY RLRDWLFSRQ RYWGEPIPVI HWEDGETTLV PEDELPLLLP
460 470 480 490 500
KATEIKPSGT GESPLANLHD WVNVTDKNGR KGRRETNTMP QWAGSSWYFL
510 520 530 540 550
RYIDPNNSEA IADKEKLAEW LPVDVYIGGA EHAVLHLLYA RFWHKFLYDI
560 570 580 590 600
GVVPTKEPFQ KLFNQGMILG ENNEKMSKSR GNVVNPDEVV EKYGADTLRL
610 620 630 640 650
YEMFMGPLEA SIAWNENGLE GARKFLDRIW RLLVTEEGTL AEKVTTDANA
660 670 680 690 700
NLEKAYHHMV KTVTNHYENL RFNTGISQLM IFINEAYKQD TIPKQYVEGF
710 720 730 740 750
VQLLSPIAPH LAEELWEILG HTETISYVAW PTYDETKLVE DEVEIVLQVN
760 770 780 790 800
GKVKSKITVA KSLEKEELEK IAQEDNKMKE NLEGKTIRKV IVVPGKLVNI

VAN
Length:803
Mass (Da):91,939
Last modified:July 5, 2004 - v1
Checksum:i0CBA3CA59982668F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017262 Genomic DNA. Translation: AAT04457.1.
RefSeqiYP_014280.1. NC_002973.6.

Genome annotation databases

EnsemblBacteriaiAAT04457; AAT04457; LMOf2365_1684.
GeneIDi2798737.
KEGGilmf:LMOf2365_1684.
PATRICi20324603. VBILisMon105049_1689.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017262 Genomic DNA. Translation: AAT04457.1.
RefSeqiYP_014280.1. NC_002973.6.

3D structure databases

ProteinModelPortaliQ71Z07.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi265669.LMOf2365_1684.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT04457; AAT04457; LMOf2365_1684.
GeneIDi2798737.
KEGGilmf:LMOf2365_1684.
PATRICi20324603. VBILisMon105049_1689.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: F2365.

Entry informationi

Entry nameiSYL_LISMF
AccessioniPrimary (citable) accession number: Q71Z07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 5, 2004
Last modified: January 7, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.