Reviewed, UniProtKB/Swiss-Prot Q71YQ4 (PUR2_LISMF)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	LMOf2365_1789

Organism

Listeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]

Taxonomic identifier

265669 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. HAMAP MF_00138
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151461
Regions
	Domain	108 – 314	207	ATP-grasp
	Nucleotide binding	134 – 195	62	ATP By similarity
Sites
	Metal binding	284	1	Magnesium or manganese By similarity
	Metal binding	286	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q71YQ4-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B1EB127211746987	FASTA	420	45,430
	10 20 30 40 50 60 MNLLVVGSGG REHAISKKLL ESNNVENVYC APGNDGMRLD NIQLVAISET DKAGLIDFAK 70 80 90 100 110 120 KAEIAFVIVG PEVPLLEGVV DALEEAGIKA FGPKANAALI EGSKDFAKQF MEKYAIPTAA 130 140 150 160 170 180 SKTFTDYAEA KAYLDERGVP IVIKADGLAA GKGVTVALEM EEAVLALKDM MLEEKFGDAS 190 200 210 220 230 240 LKVVIEDFLA GEEFSLMAFV NGEEVYPMAI AQDHKRAYEG DKGPNTGGMG AYSPVPHISE 250 260 270 280 290 300 TVVDEAVEKI LLPAAKGMVK EGRYFRGILY AGLILTAEGP KVIEFNARFG DPETQVVLPR 310 320 330 340 350 360 LESDFAALID ALLHNEKPDV RFKKSGITLG VVLASAGYPE HYEKGNKLTG LNDVAEDVAI 370 380 390 400 410 420 YHAGTKQDEN DDFISDGGRV LLLAKEAETM TDARTLLYPE MQKLDNPNFF YRIDIGTKAE

References

[1]

"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M. Nucleic Acids Res. 32:2386-2395(2004) [PubMed: 15115801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017262 Genomic DNA. Translation: AAT04560.1.
RefSeq	YP_014383.1.
3D structure databases
SMR	Q71YQ4. Positions 1-419.
ModBase	Search...
Protein-protein interaction databases
STRING	Q71YQ4.
Genome annotation databases
GeneID	2798129.
GenomeReviews	Gene locus LMOf2365_1789 in contig AE017262_GR.
KEGG	lmf:LMOf2365_1789.
TIGR	LMOf2365_1789.
Phylogenomic databases
eggNOG	COG0151.
HOGENOM	HBG404060.
OMA	FSLMAFV.
Enzyme and pathway databases
BioCyc	LMON265669:LMOF2365_1789-MONOMER.
Family and domain databases
HAMAP	MF_00138. GARS. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_LISMF

Accession

Primary (citable) accession number: Q71YQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)