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Q71YQ4 (PUR2_LISMF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:LMOf2365_1789
OrganismListeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]
Taxonomic identifier265669 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

Protein attributes

Sequence length420 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 420420Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151461

Regions

Domain108 – 314207ATP-grasp
Nucleotide binding134 – 19562ATP By similarity

Sites

Metal binding2841Magnesium or manganese By similarity
Metal binding2861Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q71YQ4 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: B1EB127211746987

FASTA42045,430
        10         20         30         40         50         60 
MNLLVVGSGG REHAISKKLL ESNNVENVYC APGNDGMRLD NIQLVAISET DKAGLIDFAK 

        70         80         90        100        110        120 
KAEIAFVIVG PEVPLLEGVV DALEEAGIKA FGPKANAALI EGSKDFAKQF MEKYAIPTAA 

       130        140        150        160        170        180 
SKTFTDYAEA KAYLDERGVP IVIKADGLAA GKGVTVALEM EEAVLALKDM MLEEKFGDAS 

       190        200        210        220        230        240 
LKVVIEDFLA GEEFSLMAFV NGEEVYPMAI AQDHKRAYEG DKGPNTGGMG AYSPVPHISE 

       250        260        270        280        290        300 
TVVDEAVEKI LLPAAKGMVK EGRYFRGILY AGLILTAEGP KVIEFNARFG DPETQVVLPR 

       310        320        330        340        350        360 
LESDFAALID ALLHNEKPDV RFKKSGITLG VVLASAGYPE HYEKGNKLTG LNDVAEDVAI 

       370        380        390        400        410        420 
YHAGTKQDEN DDFISDGGRV LLLAKEAETM TDARTLLYPE MQKLDNPNFF YRIDIGTKAE 

« Hide

References

[1]"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species."
Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. expand/collapse author list , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M.
Nucleic Acids Res. 32:2386-2395(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: F2365.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017262 Genomic DNA. Translation: AAT04560.1.
RefSeqYP_014383.1. NC_002973.6.

3D structure databases

ProteinModelPortalQ71YQ4.
SMRQ71YQ4. Positions 1-419.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING265669.LMOf2365_1789.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT04560; AAT04560; LMOf2365_1789.
GeneID2798129.
KEGGlmf:LMOf2365_1789.
PATRIC20324867. VBILisMon105049_1796.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033464.
KOK01945.
OMAYKGFLYA.
OrthoDBEOG69SKD1.

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_LISMF
AccessionPrimary (citable) accession number: Q71YQ4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways