Q71YI5 (PYRE_LISMF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	LMOf2365_1859

Organism

Listeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]

Taxonomic identifier

265669 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110707
Regions
	Region	122 – 130	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	96	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	100	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	102	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	126	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q71YI5 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: FD8607975F60EADA	FASTA	209	22,806
	10 20 30 40 50 60 MSIEKQVAEQ LLEIKAVFLK PNEPFTWASG IKSPIYCDNR LTLGFPKVRQ FIAKSLAEKI 70 80 90 100 110 120 KQTFGEVDVV AGTATAGIPH AAWVSDLLDL PMVYVRSKAK EHGKGNQIEG PISKGQKVVV 130 140 150 160 170 180 IEDLISTGGS SLKAVEALEE AGAEVVGIAA IFTYGLAKGK QLLEESGTKL VTLTNYDELI 190 200 EVALNENYVT GEDMETLKEW KKNPENWGK

References

[1]

"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M. Nucleic Acids Res. 32:2386-2395(2004) [PubMed: 15115801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F2365.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017262 Genomic DNA. Translation: AAT04629.1.
RefSeq	YP_014452.1. NC_002973.6.
3D structure databases
ProteinModelPortal	Q71YI5.
SMR	Q71YI5. Positions 1-208.
ModBase	Search...
Protein-protein interaction databases
STRING	Q71YI5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2799865.
GenomeReviews	Gene locus LMOf2365_1859 in contig AE017262_GR.
KEGG	lmf:LMOf2365_1859.
PATRIC	20325011. VBILisMon105049_1865.
TIGR	LMOf2365_1859.
Phylogenomic databases
eggNOG	COG0461.
HOGENOM	HBG404341.
OMA	LPMTYVR.
PhylomeDB	Q71YI5.
ProtClustDB	PRK00455.
Enzyme and pathway databases
BioCyc	LMON265669:LMOF2365_1859-MONOMER.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR004467. Or_phspho_trans_clade-1. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. PyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_LISMF

Accession

Primary (citable) accession number: Q71YI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program