Reviewed, UniProtKB/Swiss-Prot Q71YI0 (CARA_LISMF)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	LMOf2365_1864

Organism

Listeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]

Taxonomic identifier

265669 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity. HAMAP MF_01209
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112291
Regions
	Domain	172 – 359	188	Glutamine amidotransferase type-1
	Region	1 – 168	168	CPSase HAMAP MF_01209
Sites
	Active site	247	1	Nucleophile By similarity
	Active site	332	1	By similarity
	Active site	334	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q71YI0-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 926B5F54F4C7142A	FASTA	363	40,095
	10 20 30 40 50 60 MTKRILMLED GNYFIGDAIG SEKETIGEVV FNTGMTGYQE TITDPSYYGQ IITFTYPLVG 70 80 90 100 110 120 NYGVNRDDFE SINPAVKGVV VREAAEFPSN WRNQITLDEF LKEKGIPGIA GIDTRKLTKL 130 140 150 160 170 180 IRKEGTLKGI LAAETANKEE LLHHLRSVRL PVDQVHEVSS AKAFASPGDG KRVVLVDYGV 190 200 210 220 230 240 KSSILRELNK RNCYVTVVPY NTTAEEILAM HPDGVMLSNG PGDPKDVPEA LEMIRGIQGK 250 260 270 280 290 300 LPLFGICLGH QLFALANGAD TFKLKFGHRG ANHPVKELAT GRVDFTAQNH GYAVEKDSLI 310 320 330 340 350 360 GTDLKVTHIE LNDETVEGLA HKEYPAYTVQ YHPEANPGPS DVNYLFDEFM EMMNGKEEGE LHA

References

[1]

"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M. Nucleic Acids Res. 32:2386-2395(2004) [PubMed: 15115801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017262 Genomic DNA. Translation: AAT04634.1.
RefSeq	YP_014457.1.
3D structure databases
SMR	Q71YI0. Positions 1-357.
ModBase	Search...
Protein-protein interaction databases
STRING	Q71YI0.
Genome annotation databases
GeneID	2797633.
GenomeReviews	Gene locus LMOf2365_1864 in contig AE017262_GR.
KEGG	lmf:LMOf2365_1864.
TIGR	LMOf2365_1864.
Phylogenomic databases
eggNOG	COG0505.
HOGENOM	HBG286341.
OMA	FTYPELG.
Enzyme and pathway databases
BioCyc	LMON265669:LMOF2365_1864-MONOMER.
Family and domain databases
HAMAP	MF_01209_B. CPSase_S_chain_B. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase_dom. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_LISMF

Accession

Primary (citable) accession number: Q71YI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)