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Protein

Asparagine--tRNA ligase

Gene

asnS

Organism
Listeria monocytogenes serotype 4b (strain F2365)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine--tRNA ligaseUniRule annotation (EC:6.1.1.22UniRule annotation)
Alternative name(s):
Asparaginyl-tRNA synthetaseUniRule annotation
Short name:
AsnRSUniRule annotation
Gene namesi
Name:asnSUniRule annotation
Ordered Locus Names:LMOf2365_1925
OrganismiListeria monocytogenes serotype 4b (strain F2365)
Taxonomic identifieri265669 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 430430Asparagine--tRNA ligasePRO_0000176423Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ71YB9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000226034.
KOiK01893.
OMAiMEHRHLW.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00534. Asn_tRNA_synth. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-ligase.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF16. PTHR22594:SF16. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00457. asnS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q71YB9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKITINQASE FVGKEVTIGA WLANKRSSGK IAFLQLRDGT GFMQGVVVKA
60 70 80 90 100
EVGDDIFATA KALTQETSLY VTGTINEDTR SPFGYEMAVS GVEVISESHD
110 120 130 140 150
YPITPKEHGT EFLMDHRHLW LRSNRQHAIM KIRNEIIRAS YEFFNKEGFL
160 170 180 190 200
KIDPPILTGS APEGTTELFH TKYFDEDAFL SQSGQLYMEA AAMAFGKVFS
210 220 230 240 250
FGPTFRAEKS KTRRHLIEFW MIEPEMAFYK LEDSLQVQEN YVAFLVKAVL
260 270 280 290 300
DNCRLELDRL GRDVSHLEKM VAPFPRITYT EAIERLHELG FDDIVWGDDF
310 320 330 340 350
GAPHETAIAD SFEKPVFITH YPKAIKPFYM PEDPENDQVV LCADMIAPEG
360 370 380 390 400
YGEIIGGSER IHDLETLQAR MEDFDLDQEA YSWYLDLARY GSVPHSGFGL
410 420 430
GLERTVAWIS GTEHVRETIP FPRLLNRLYP
Length:430
Mass (Da):48,955
Last modified:July 5, 2004 - v1
Checksum:i6F1527B97829BDA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017262 Genomic DNA. Translation: AAT04695.1.
RefSeqiWP_003726759.1. NC_002973.6.

Genome annotation databases

EnsemblBacteriaiAAT04695; AAT04695; LMOf2365_1925.
KEGGilmf:LMOf2365_1925.
PATRICi20325143. VBILisMon105049_1931.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017262 Genomic DNA. Translation: AAT04695.1.
RefSeqiWP_003726759.1. NC_002973.6.

3D structure databases

ProteinModelPortaliQ71YB9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT04695; AAT04695; LMOf2365_1925.
KEGGilmf:LMOf2365_1925.
PATRICi20325143. VBILisMon105049_1931.

Phylogenomic databases

HOGENOMiHOG000226034.
KOiK01893.
OMAiMEHRHLW.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00534. Asn_tRNA_synth. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-ligase.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF16. PTHR22594:SF16. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00457. asnS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYN_LISMF
AccessioniPrimary (citable) accession number: Q71YB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.