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Q71XX5 (MURE_LISMF) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
EC=6.3.2.13
Alternative name(s):
Meso-A2pm-adding enzyme
Meso-diaminopimelate-adding enzyme
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
UDP-MurNAc-tripeptide synthetase
UDP-N-acetylmuramyl-tripeptide synthetase
Gene names
Name:murE
Ordered Locus Names:LMOf2365_2070
OrganismListeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]
Taxonomic identifier265669 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP MF_00208

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208

Subcellular location

Cytoplasm By similarity HAMAP MF_00208.

Post-translational modification

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP MF_00208

Sequence similarities

Belongs to the MurCDEF family. MurE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208
PRO_0000101910

Regions

Nucleotide binding108 – 1147ATP Potential
Region150 – 1512UDP-MurNAc-L-Ala-D-Glu binding By similarity
Region407 – 4104Meso-diaminopimelate binding By similarity
Motif407 – 4104Meso-diaminopimelate recognition motif HAMAP MF_00208

Sites

Binding site301UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1491UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1771UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1831UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1851UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site3831Meso-diaminopimelate By similarity
Binding site4581Meso-diaminopimelate; via carbonyl oxygen By similarity
Binding site4621Meso-diaminopimelate By similarity

Amino acid modifications

Modified residue2171N6-carboxylysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q71XX5 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 1145D53BF9CD4BFD

FASTA49153,979
        10         20         30         40         50         60 
MKLNELMQAI PVFTGEASET IEISHIAQDS RKVKPGTLFI CIDGELVDGH QFASRAVELG 

        70         80         90        100        110        120 
AVAIIAEKQL DVSIPVIYVR DSKRAMAMLA DYFYGSPTQA LKLVGITGTN GKTTVSHLVE 

       130        140        150        160        170        180 
QIVRENGEQT GLIGTMYRKI GDQILETKNT TPDSLTLQET FRDMLLSGVS TAVMEVSSHA 

       190        200        210        220        230        240 
LVQGRVYGSD YDVAVFMNLS QDHLDYHHTM EEYANAKSLL FAQLGNSYHT SNPKIAVLNA 

       250        260        270        280        290        300 
DDEESVRMQK ATAAHVITFG IKQQADFKAN NIRITSHGST FDLRTPIGNF TLKIKMIGNF 

       310        320        330        340        350        360 
SVYNVLAAIA TSFALHIPME KAIKTVESIP GVKGRFELVH AGQEFPVIVD YAHTPDGLLN 

       370        380        390        400        410        420 
VLETIAEFAE KRVFVVVGCG GDRDKGKRPQ MAKIAVDYAT NPIFTSDNPR SENPRAIIED 

       430        440        450        460        470        480 
MIQGVPYSDS YVVHENRRDA IRFAVNEAEA GDVILIAGKG HEDYQVIGDE VIDFDDRVEA 

       490 
RIAIEKKLGL A

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References

[1]"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species."
Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. expand/collapse author list , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M.
Nucleic Acids Res. 32:2386-2395(2004) [PubMed: 15115801] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: F2365.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017262 Genomic DNA. Translation: AAT04840.1.
RefSeqYP_014663.1. NC_002973.6.

3D structure databases

ProteinModelPortalQ71XX5.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ71XX5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2797029.
GenomeReviewsGene locus LMOf2365_2070 in contig AE017262_GR.
KEGGlmf:LMOf2365_2070.
PATRIC20325435. VBILisMon105049_2077.
TIGRLMOf2365_2070.

Phylogenomic databases

eggNOGCOG0769.
HOGENOMHBG602753.
OMASCANAAG.
PhylomeDBQ71XX5.
ProtClustDBPRK00139.

Enzyme and pathway databases

BioCycLMON265669:LMOF2365_2070-MONOMER.

Family and domain databases

HAMAPMF_00208. MurE.
[Tree]
InterProIPR018109. Folylpolyglutamate_synth_CS.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
KOK01928.
PfamPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMsTIGR01085. MurE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMURE_LISMF
AccessionPrimary (citable) accession number: Q71XX5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 5, 2004
Last modified: January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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