Q71XS4 (ASSY_LISMF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Argininosuccinate synthase
EC=6.3.4.5

Alternative name(s):
Citrulline--aspartate ligase

Gene names

Name:	argG
Ordered Locus Names:	LMOf2365_2122

Organism

Listeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]

Taxonomic identifier

265669 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Listeriaceae › Listeria › Listeria monocytogenes

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP argininosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	Argininosuccinate synthase HAMAP-Rule MF_00005		PRO_0000148609
Regions
	Nucleotide binding	9 – 17	9	ATP By similarity
Sites
	Binding site	86	1	Citrulline By similarity
	Binding site	116	1	ATP; via amide nitrogen By similarity
	Binding site	118	1	Aspartate By similarity
	Binding site	122	1	Aspartate By similarity
	Binding site	122	1	Citrulline By similarity
	Binding site	123	1	Aspartate By similarity
	Binding site	126	1	Citrulline By similarity
	Binding site	174	1	Citrulline By similarity
	Binding site	183	1	Citrulline By similarity
	Binding site	259	1	Citrulline By similarity
	Binding site	271	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q71XS4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: D3A516157056D70F	FASTA	404	44,478
	10 20 30 40 50 60 MAKEKIVLAY SGGLDTSVAI QWLVESGYEV IACCLDVGEG KNLDFIKEKA ITVGASESYT 70 80 90 100 110 120 IDAKEEFAED FALIALQAHA YYEGKYPLIS ALSRPLIAKK LVEVARQEGA SAIAHGCTGK 130 140 150 160 170 180 GNDQVRFEVA IHALAPDLKV VSPVRDWKWS REEEINYAKE HNIPVPIDLD NPFSIDQNLW 190 200 210 220 230 240 GRSNECGVLE NPWTTPPEAA YDLTVSLEDA PDTPDIVEIT FDAGIPISLN GENMSLANLI 250 260 270 280 290 300 LTLNEIAGKH GVGRIDHIEN RLVGIKSREV YECPAAVTLI TAHKELEDLT FVREVAHFKP 310 320 330 340 350 360 IIEQKISETI YNGLWFSPLT EALVAFLKST QKFVNGTIRV KLFKGHAIVE GRKSPNSLYD 370 380 390 400 ENLATYTSSD TFDQDAAVGF IKLWGLPTKV SAEVNSKVTI TTEV

References

[1]

"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M. Nucleic Acids Res. 32:2386-2395(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F2365.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017262 Genomic DNA. Translation: AAT04891.1.
RefSeq	YP_014714.1. NC_002973.6.
3D structure databases
ProteinModelPortal	Q71XS4.
ModBase	Search...
Protein-protein interaction databases
STRING	265669.LMOf2365_2122.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAT04891; AAT04891; LMOf2365_2122.
GeneID	2797067.
KEGG	lmf:LMOf2365_2122.
PATRIC	20325547. VBILisMon105049_2132.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0137.
HOGENOM	HOG000230093.
KO	K01940.
OMA	NDQFRFE.
ProtClustDB	PRK00509.
Enzyme and pathway databases
UniPathway	UPA00068; UER00113.
Family and domain databases
Gene3D	3.40.50.620. 1 hit. 3.90.1260.10. 1 hit.
HAMAP	MF_00005. Arg_succ_synth_type1.
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. IPR023434. Arginosuc_synth_type_1_subfam. IPR024074. AS_cat/multimer_dom_body. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR11587. PTHR11587. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. argG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY_LISMF

Accession

Primary (citable) accession number: Q71XS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program