Reviewed, UniProtKB/Swiss-Prot Q71XE0 (FUMC_LISMF)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	LMOf2365_2258

Organism

Listeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]

Taxonomic identifier

265669 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP MF_00743
Subunit structure	Homotetramer By similarity. HAMAP MF_00743
Subcellular location	Cytoplasm By similarity HAMAP MF_00743.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity. HAMAP MF_00743
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 455	455	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161285
Regions
	Region	122 – 125	4	B site By similarity
	Region	132 – 134	3	Substrate binding By similarity
Sites
	Binding site	98	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q71XE0-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: F471032E5D146F5C	FASTA	455	49,635
	10 20 30 40 50 60 MERIERDTLG EISVDATKYW GAQTERSKRN FAIGDNPMPI EIIYAFAQLK KATAKVNAAE 70 80 90 100 110 120 GKLSEEKAIA IGQVCDQIIQ GELDEHFPLV VWQTGSGTQS NMNVNEVIAH VANLTLGEGQ 130 140 150 160 170 180 IHPNDDVNMS QSSNDTFPTA MHIAAYGALV TKLLPEITKM EAVLAEKKSK YMHLVKIGRT 190 200 210 220 230 240 HLQDATPLTL GQEISGWEAC LTNNKNYLET SMKAILPLAI GGTAVGTGLN ASRDFGDKVA 250 260 270 280 290 300 EELMKQTGYP FTSDSNKYFA LTSHSPINFV HGSIRSLASD LMKIANDIRL LASGPRSGIG 310 320 330 340 350 360 ELTIPVNEPG SSIMPGKVNP TQCEAMTMVA AQVMGNDVTI NVAASQGNFE LNVYKPVIIF 370 380 390 400 410 420 NFLESVKLLS DSMRSFRLHC LEGLTANEKV IETKVNDSLM LVTALNPHIG YEKAAKIAKL 430 440 450 AFDENTTLKE AAIKTGFVTE KQFDLWIDPL KMTNL

References

[1]

"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M. Nucleic Acids Res. 32:2386-2395(2004) [PubMed: 15115801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017262 Genomic DNA. Translation: AAT05025.1.
RefSeq	YP_014848.1.
3D structure databases
SMR	Q71XE0. Positions 3-452.
ModBase	Search...
Protein-protein interaction databases
STRING	Q71XE0.
Genome annotation databases
GeneID	2799834.
GenomeReviews	Gene locus LMOf2365_2258 in contig AE017262_GR.
KEGG	lmf:LMOf2365_2258.
TIGR	LMOf2365_2258.
Phylogenomic databases
eggNOG	COG0114.
HOGENOM	HBG284369.
OMA	RIEKDTM.
Enzyme and pathway databases
BioCyc	LMON265669:LMOF2365_2258-MONOMER.
Family and domain databases
HAMAP	MF_00743. FumaraseC. [Tree]
InterPro	IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_LISMF

Accession

Primary (citable) accession number: Q71XE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)