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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Listeria monocytogenes serotype 4b (strain F2365)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei180SubstrateUniRule annotation1
Active sitei181Proton donor/acceptorUniRule annotation1
Active sitei311UniRule annotation1
Binding sitei312SubstrateUniRule annotation1
Sitei324Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:LMOf2365_2258
OrganismiListeria monocytogenes serotype 4b (strain F2365)
Taxonomic identifieri265669 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001612851 – 455Fumarate hydratase class IIAdd BLAST455

Proteomic databases

PRIDEiQ71XE0

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ71XE0
SMRiQ71XE0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni96 – 98Substrate bindingUniRule annotation3
Regioni122 – 125Substrate binding (B site)UniRule annotation4
Regioni132 – 134Substrate bindingUniRule annotation3
Regioni317 – 319Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000061736
KOiK01679
OMAiVSFTDNC

Family and domain databases

CDDicd01362 Fumarase_classII, 1 hit
Gene3Di1.10.275.10, 1 hit
HAMAPiMF_00743 FumaraseC, 1 hit
InterProiView protein in InterPro
IPR005677 Fum_hydII
IPR024083 Fumarase/histidase_N
IPR018951 Fumarase_C_C
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR11444 PTHR11444, 1 hit
PfamiView protein in Pfam
PF10415 FumaraseC_C, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR00979 fumC_II, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequencei

Sequence statusi: Complete.

Q71XE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERIERDTLG EISVDATKYW GAQTERSKRN FAIGDNPMPI EIIYAFAQLK
60 70 80 90 100
KATAKVNAAE GKLSEEKAIA IGQVCDQIIQ GELDEHFPLV VWQTGSGTQS
110 120 130 140 150
NMNVNEVIAH VANLTLGEGQ IHPNDDVNMS QSSNDTFPTA MHIAAYGALV
160 170 180 190 200
TKLLPEITKM EAVLAEKKSK YMHLVKIGRT HLQDATPLTL GQEISGWEAC
210 220 230 240 250
LTNNKNYLET SMKAILPLAI GGTAVGTGLN ASRDFGDKVA EELMKQTGYP
260 270 280 290 300
FTSDSNKYFA LTSHSPINFV HGSIRSLASD LMKIANDIRL LASGPRSGIG
310 320 330 340 350
ELTIPVNEPG SSIMPGKVNP TQCEAMTMVA AQVMGNDVTI NVAASQGNFE
360 370 380 390 400
LNVYKPVIIF NFLESVKLLS DSMRSFRLHC LEGLTANEKV IETKVNDSLM
410 420 430 440 450
LVTALNPHIG YEKAAKIAKL AFDENTTLKE AAIKTGFVTE KQFDLWIDPL

KMTNL
Length:455
Mass (Da):49,635
Last modified:July 5, 2004 - v1
Checksum:iF471032E5D146F5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017262 Genomic DNA Translation: AAT05025.1
RefSeqiWP_010959021.1, NC_002973.6

Genome annotation databases

EnsemblBacteriaiAAT05025; AAT05025; LMOf2365_2258
KEGGilmf:LMOf2365_2258

Similar proteinsi

Entry informationi

Entry nameiFUMC_LISMF
AccessioniPrimary (citable) accession number: Q71XE0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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