Reviewed, UniProtKB/Swiss-Prot Q71WM7 (MURA2_LISMF)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2
    EC=2.5.1.7
Alternative name(s):
    Enoylpyruvate transferase 2
    UDP-N-acetylglucosamine enolpyruvyl transferase 2
      Short name=EPT 2

Gene names

Name:	murA2
Synonyms:	murA-2, murZ
Ordered Locus Names:	LMOf2365_2524

Organism

Listeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]

Taxonomic identifier

265669 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity.
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 HAMAP MF_00111		PRO_0000178886
Sites
	Active site	117	1	Proton donor By similarity
	Binding site	117	1	Phosphoenolpyruvate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q71WM7-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 95218B3A10B51A6F	FASTA	423	45,056
	10 20 30 40 50 60 MTDKLIIRGG KKLAGTLQVD GAKNSAVALI PAAILAESEV VLEGLPDISD VHTLYNILEE 70 80 90 100 110 120 LGGTVRYDNK TAVIDPTDMI SMPLPSGNVK KLRASYYLMG AMLGRFKKAV IGLPGGCYLG 130 140 150 160 170 180 PRPIDQHIKG FEALGAKVTN EQGAIYLRAD ELKGARIYLD VVSVGATINI MLAAVRAKGK 190 200 210 220 230 240 TVIENAAKEP EIIDVATLLT NMGAIIKGAG TDTIRITGVE HLHGCHHTII PDRIEAGTFM 250 260 270 280 290 300 VLAAASGKGV RIENVIPTHL EGIIAKLTEM GVPMDIEEDA IFVGEVEKIK KVDIKTYAYP 310 320 330 340 350 360 GFPTDLQQPL TALLTRAEGS SVITDTIYPS RFKHIAEIER MGGKFKLEGR SAVINGPVQL 370 380 390 400 410 420 QGSKVTATDL RAGAALVIAA LLADGETEIH GVEHIERGYS KIIEKLSAIG ANITRSSAAE TKL

References

[1]

"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M. Nucleic Acids Res. 32:2386-2395(2004) [PubMed: 15115801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017262 Genomic DNA. Translation: AAT05289.1.
RefSeq	YP_015112.1.
3D structure databases
HSSP	HSSP built from PDB template 1A2N based on UniProtKB P28909.
ModBase	Search...
Protein-protein interaction databases
STRING	Q71WM7.
Genome annotation databases
GeneID	2798687.
GenomeReviews	Gene locus LMOf2365_2524 in contig AE017262_GR.
KEGG	lmf:LMOf2365_2524.
TIGR	LMOf2365_2524.
Phylogenomic databases
HOGENOM	Q71WM7.
OMA	DNVIPTH.
Enzyme and pathway databases
BioCyc	LMON265669:LMOF2365_2524-MON.
Family and domain databases
HAMAP	MF_00111. [Tree]
InterPro	IPR001986. EPSP_synthase_core. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
ProDom	PD001867. EPSP_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA2_LISMF

Accession

Primary (citable) accession number: Q71WM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)