SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q71VZ1

- GSHAB_LISMF

UniProt

Q71VZ1 - GSHAB_LISMF

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Glutathione biosynthesis bifunctional protein GshAB
Gene
gshAB, gshF, LMOf2365_2760
Organism
Listeria monocytogenes serotype 4b (strain F2365)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi721 – 7211Magnesium or manganese 1 By similarity
Metal bindingi738 – 7381Magnesium or manganese 1 By similarity
Metal bindingi738 – 7381Magnesium or manganese 2 By similarity
Metal bindingi740 – 7401Magnesium or manganese 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi541 – 59959ATP By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
  3. glutathione synthase activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshAB
Alternative name(s):
Gamma-GCS-GS
Short name:
GCS-GS
Including the following 2 domains:
Glutamate--cysteine ligase (EC:6.3.2.2)
Alternative name(s):
Gamma-ECS
Short name:
GCS
Gamma-glutamylcysteine synthetase
Glutathione synthetase (EC:6.3.2.3)
Alternative name(s):
GSH synthetase
Short name:
GS
Short name:
GSH-S
Short name:
GSHase
Glutathione synthase
Gene namesi
Name:gshAB
Synonyms:gshF
Ordered Locus Names:LMOf2365_2760
OrganismiListeria monocytogenes serotype 4b (strain F2365)
Taxonomic identifieri265669 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000563: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 769769Glutathione biosynthesis bifunctional protein GshABUniRule annotation
PRO_0000192554Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi265669.LMOf2365_2760.

Structurei

3D structure databases

ProteinModelPortaliQ71VZ1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini514 – 768255ATP-grasp
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 347347Glutamate--cysteine ligaseUniRule annotation
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.
Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000156471.
KOiK01919.
OrthoDBiEOG6BKJ7H.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 3 hits.
HAMAPiMF_00782. Glut_biosynth.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
[Graphical view]
PfamiPF01071. GARS_A. 1 hit.
PF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q71VZ1-1 [UniParc]FASTAAdd to Basket

« Hide

MLDSFKEDPK LRKLLFSGHF GLEKENIRVT SDGKLALTPH PAIFGPKEDN    50
PYIKTDFSES QIEMITPVTD SIDSVYEWLE NLHNIVSLRS ENELLWPSSN 100
PPILPAEEDI PIAEYKTPDS PDRKYREHLA KGYGKKIQLL SGIHYNFSFP 150
EALIDGLYDK ISLPEESKQD FKNRLYLKVA KYFMKNRWLL IYLTGASPVY 200
LADFSKTKHD ESLPDGSSAL RDGISLRNSN AGYKNKEALY VDYNSFDAYI 250
SSISNYIEAG KIESMREFYN PIRLKNAHTD QTVESLAEHG VEYLEIRSID 300
LNPLESNGIS KDELAFIHLF LIKGLLSEDR ELCSNNQQLA DENENNIALN 350
GLAQPALKNC DNEEISVVEA GLLELNKMSD FIQSLRPEDT KLQAIIEKQK 400
ERLLHPEKTI AAQVKQQVTK EGYVDFHLNQ AKTYMEETEA LAYKLIGAED 450
MELSTQIIWK DAIARGIKVD VLDRAENFLR FQKGDHVEYV KQASKTSKDN 500
YVSVLMMENK VVTKLVLAEH DIRVPFGDSF SDQALALEAF SLFEDKQIVV 550
KPKSTNYGWG ISIFKNKFTL EDYQEALNIA FSYDSSVIIE EFIPGDEFRF 600
LVINDKVEAV LKRVPANVTG DGIHTVRELV EEKNTDPLRG TDHLKPLEKI 650
RTGPEETLML SMQNLSWDSI PKAEEIIYLR ENSNVSTGGD SIDYTEEMDD 700
YFKEIAIRAT QVLDAKICGV DIIVPRETID RDKHAIIELN FNPAMHMHCF 750
PYQGEKKKIG DKILDFLFE 769
Length:769
Mass (Da):87,751
Last modified:July 5, 2005 - v2
Checksum:i94140E0923FCD379
GO

Sequence cautioni

The sequence AAT05525.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017262 Genomic DNA. Translation: AAT05525.1. Different initiation.
RefSeqiYP_015348.1. NC_002973.6.

Genome annotation databases

EnsemblBacteriaiAAT05525; AAT05525; LMOf2365_2760.
GeneIDi2797638.
KEGGilmf:LMOf2365_2760.
PATRICi20326908. VBILisMon105049_2779.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017262 Genomic DNA. Translation: AAT05525.1 . Different initiation.
RefSeqi YP_015348.1. NC_002973.6.

3D structure databases

ProteinModelPortali Q71VZ1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 265669.LMOf2365_2760.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT05525 ; AAT05525 ; LMOf2365_2760 .
GeneIDi 2797638.
KEGGi lmf:LMOf2365_2760.
PATRICi 20326908. VBILisMon105049_2779.

Phylogenomic databases

eggNOGi COG1181.
HOGENOMi HOG000156471.
KOi K01919.
OrthoDBi EOG6BKJ7H.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00209 .
UPA00142 ; UER00210 .

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 3 hits.
HAMAPi MF_00782. Glut_biosynth.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
[Graphical view ]
Pfami PF01071. GARS_A. 1 hit.
PF04262. Glu_cys_ligase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: F2365.

Entry informationi

Entry nameiGSHAB_LISMF
AccessioniPrimary (citable) accession number: Q71VZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 5, 2005
Last modified: May 14, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi