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Q71VM4 (IMA1A_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Importin subunit alpha-1a
Gene names
Ordered Locus Names:Os01g0253300, LOC_Os01g14950
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length526 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions in nuclear protein import. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Promotes docking of import substrates to the nuclear envelope. Ref.4 Ref.5

Subunit structure

Forms a complex with importin subunit beta-1. The whole complex, most stable and composed of importin alpha, importin beta and NLS substrate, is referred to as PTAC or pore targeting complex. Interacts with mungbean yellow mosaic virus capsid protein. Ref.5 Ref.6

Subcellular location

Cytoplasmperinuclear region Ref.4.

Tissue specificity

Highly expressed in callus, followed by root and etiolated leaf. Low expression in green leaf. Ref.1 Ref.5

Induction

In both etiolated and green leaves, down-regulated by light. In green leaf, increased by dark treatment. Ref.1 Ref.5

Sequence similarities

Belongs to the importin alpha family.

Contains 8 ARM repeats.

Contains 1 IBB domain.

Ontologies

Keywords
   Biological processHost-virus interaction
Protein transport
Transport
   Cellular componentCytoplasm
   DomainRepeat
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdefense response

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

pollen sperm cell differentiation

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

protein import into nucleus

Inferred from electronic annotation. Source: InterPro

viral process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcell wall

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

cytosol

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

nuclear envelope

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

nucleolus

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasmodesma

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionprotein transporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 526526Importin subunit alpha-1a
PRO_0000120740

Regions

Domain1 – 5858IBB
Repeat105 – 14541ARM 1
Repeat148 – 18740ARM 2
Repeat190 – 23041ARM 3
Repeat232 – 27140ARM 4
Repeat274 – 31340ARM 5
Repeat316 – 35641ARM 6
Repeat359 – 39840ARM 7
Repeat402 – 44140ARM 8

Experimental info

Sequence conflict473 – 4742LQ → FE in AAQ13406. Ref.2

Secondary structure

.................................................................. 526
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q71VM4 [UniParc].

Last modified April 26, 2005. Version 2.
Checksum: 7A7DA264968FC983

FASTA52657,571
        10         20         30         40         50         60 
MSLRPSERVE VRRNRYKVAV DAEEGRRRRE DNMVEIRKSR REESLLKKRR EGLQAQAPVP 

        70         80         90        100        110        120 
ASAATGVDKK LESLPAMIGG VYSDDNNLQL EATTQFRKLL SIERSPPIEE VIQSGVVPRF 

       130        140        150        160        170        180 
VQFLTREDFP QLQFEAAWAL TNIASGTSEN TKVVIDHGAV PIFVKLLGSS SDDVREQAVW 

       190        200        210        220        230        240 
ALGNVAGDSP KCRDLVLANG ALLPLLAQLN EHTKLSMLRN ATWTLSNFCR GKPQPSFEQT 

       250        260        270        280        290        300 
RPALPALARL IHSNDEEVLT DACWALSYLS DGTNDKIQAV IEAGVCPRLV ELLLHPSPSV 

       310        320        330        340        350        360 
LIPALRTVGN IVTGDDAQTQ CIIDHQALPC LLSLLTQNLK KSIKKEACWT ISNITAGNKD 

       370        380        390        400        410        420 
QIQAVINAGI IGPLVNLLQT AEFDIKKEAA WAISNATSGG SHDQIKYLVS EGCIKPLCDL 

       430        440        450        460        470        480 
LICPDIRIVT VCLEGLENIL KVGETDKTLA AGDVNVFSQM IDEAEGLEKI ENLQSHDNNE 

       490        500        510        520 
IYEKAVKILE AYWMDEEDDT MGATTVAAPQ GATFDFGQGG GAAQFK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of a cDNA encoding an importin-alpha and down-regulation of the gene by light in rice leaves."
Shoji K., Iwasaki T., Matsuki R., Miyao M., Yamamoto N.
Gene 212:279-286(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, INDUCTION.
Strain: cv. Nipponbare.
Tissue: Callus and Leaf.
[2]"Importin alpha-3, a novel variant of the small importin subunit evolutionarily conserved from hydrozoa to man."
Fischer R.
Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The genome sequence and structure of rice chromosome 1."
Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. expand/collapse author list , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"Functional characterization of a plant importin alpha homologue. Nuclear localization signal (NLS)-selective binding and mediation of nuclear import of nls proteins in vitro."
Jiang C.-J., Imamoto N., Matsuki R., Yoneda Y., Yamamoto N.
J. Biol. Chem. 273:24083-24087(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"Molecular cloning of a novel importin alpha homologue from rice, by which constitutive photomorphogenic 1 (COP1) nuclear localization signal (NLS)-protein is preferentially nuclear imported."
Jiang C.-J., Shoji K., Matsuki R., Baba A., Inagaki N., Ban H., Iwasaki T., Imamoto N., Yoneda Y., Deng X.-W., Yamamoto N.
J. Biol. Chem. 276:9322-9329(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION, INTERACTION WITH IMPORTIN BETA-1.
[6]"Coat proteins of Rice tungro bacilliform virus and Mungbean yellow mosaic virus contain multiple nuclear-localization signals and interact with importin alpha."
Guerra-Peraza O., Kirk D., Seltzer V., Veluthambi K., Schmit A.C., Hohn T., Herzog E.
J. Gen. Virol. 86:1815-1826(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MUNGBEAN YELLOW MOSAIC VIRUS CAPSID PROTEIN.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB004660 mRNA. Translation: BAA31165.1.
AF005265 mRNA. Translation: AAQ13406.1.
AB004814 Genomic DNA. Translation: BAA31166.1.
AP000816 Genomic DNA. Translation: BAA87855.1.
RefSeqNP_001042611.1. NM_001049146.1.
UniGeneOs.3405.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2YNSX-ray2.10A/B73-526[»]
4B8JX-ray2.00A1-526[»]
4B8OX-ray2.08A73-526[»]
4B8PX-ray2.30A/B73-526[»]
4BPLX-ray2.30A73-526[»]
4BQKX-ray2.00A/B73-526[»]
ProteinModelPortalQ71VM4.
SMRQ71VM4. Positions 77-496.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ71VM4. 2 interactions.
MINTMINT-6948326.

Proteomic databases

PRIDEQ71VM4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS01T0253300-01; OS01T0253300-01; OS01G0253300.
OS01T0253300-02; OS01T0253300-02; OS01G0253300.
GeneID4327117.
KEGGosa:4327117.

Organism-specific databases

GrameneQ71VM4.

Phylogenomic databases

eggNOGCOG5064.
HOGENOMHOG000167616.
OMAGGSHDQI.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR002652. Importin-a_IBB.
IPR024931. Importing_su_alpha.
[Graphical view]
PfamPF00514. Arm. 8 hits.
PF01749. IBB. 1 hit.
[Graphical view]
PIRSFPIRSF005673. Importin_alpha. 1 hit.
SMARTSM00185. ARM. 8 hits.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
PROSITEPS50176. ARM_REPEAT. 4 hits.
PS51214. IBB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIMA1A_ORYSJ
AccessionPrimary (citable) accession number: Q71VM4
Secondary accession number(s): O82783
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: April 16, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PDB cross-references

Index of Protein Data Bank (PDB) cross-references