Q71VM4 (IMA1A_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Importin subunit alpha-1a

Gene names

Ordered Locus Names:

Os01g0253300, LOC_Os01g14950

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions in nuclear protein import. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Promotes docking of import substrates to the nuclear envelope. Ref.4 Ref.5
Subunit structure	Forms a complex with importin subunit beta-1. The whole complex, most stable and composed of importin alpha, importin beta and NLS substrate, is referred to as PTAC or pore targeting complex. Interacts with mungbean yellow mosaic virus capsid protein. Ref.5 Ref.6
Subcellular location	Cytoplasm › perinuclear region Ref.4.
Tissue specificity	Highly expressed in callus, followed by root and etiolated leaf. Low expression in green leaf. Ref.1 Ref.5
Induction	In both etiolated and green leaves, down-regulated by light. In green leaf, increased by dark treatment. Ref.1 Ref.5
Sequence similarities	Belongs to the importin alpha family. Contains 8 ARM repeats. Contains 1 IBB domain.

Ontologies

Keywords
Biological process	Host-virus interaction Protein transport Transport
Cellular component	Cytoplasm
Domain	Repeat
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein import into nucleus Inferred from electronic annotation. Source: InterPro virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell wall Inferred from electronic annotation. Source: EnsemblPlants/Gramene cytosol Inferred from electronic annotation. Source: EnsemblPlants/Gramene nuclear envelope Inferred from electronic annotation. Source: EnsemblPlants/Gramene nucleolus Inferred from electronic annotation. Source: EnsemblPlants/Gramene perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 526

526

Importin subunit alpha-1a

PRO_0000120740

Regions

Domain

1 – 58

58

IBB

Repeat

105 – 145

41

ARM 1

Repeat

148 – 187

40

ARM 2

Repeat

190 – 230

41

ARM 3

Repeat

232 – 271

40

ARM 4

Repeat

274 – 313

40

ARM 5

Repeat

316 – 356

41

ARM 6

Repeat

359 – 398

40

ARM 7

Repeat

402 – 441

40

ARM 8

Experimental info

Sequence conflict

473 – 474

2

LQ → FE in AAQ13406. Ref.2

Secondary structure

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526

Helix Strand Turn

Details...

Helix

74 – 81

8

Helix

86 – 100

15

Beta strand

103 – 105

3

Helix

108 – 113

6

Helix

117 – 124

8

Helix

130 – 144

15

Helix

148 – 156

9

Helix

159 – 166

8

Helix

172 – 187

16

Helix

190 – 198

9

Helix

202 – 207

6

Helix

215 – 229

15

Beta strand

231 – 233

3

Helix

237 – 240

4

Helix

243 – 250

8

Helix

256 – 269

14

Beta strand

271 – 273

3

Helix

274 – 282

9

Helix

286 – 292

7

Helix

298 – 311

14

Helix

316 – 323

8

Turn

324 – 326

3

Helix

327 – 336

10

Helix

341 – 355

15

Helix

359 – 367

9

Helix

371 – 380

10

Helix

383 – 399

17

Helix

402 – 410

9

Helix

414 – 419

6

Helix

420 – 422

3

Helix

426 – 450

25

Helix

456 – 463

8

Helix

466 – 472

7

Helix

473 – 475

3

Helix

479 – 492

14

Sequences

	Sequence		Length	Mass (Da)
	Q71VM4 [UniParc]. Last modified April 26, 2005. Version 2. Checksum: 7A7DA264968FC983	FASTA	526	57,571
	10 20 30 40 50 60 MSLRPSERVE VRRNRYKVAV DAEEGRRRRE DNMVEIRKSR REESLLKKRR EGLQAQAPVP 70 80 90 100 110 120 ASAATGVDKK LESLPAMIGG VYSDDNNLQL EATTQFRKLL SIERSPPIEE VIQSGVVPRF 130 140 150 160 170 180 VQFLTREDFP QLQFEAAWAL TNIASGTSEN TKVVIDHGAV PIFVKLLGSS SDDVREQAVW 190 200 210 220 230 240 ALGNVAGDSP KCRDLVLANG ALLPLLAQLN EHTKLSMLRN ATWTLSNFCR GKPQPSFEQT 250 260 270 280 290 300 RPALPALARL IHSNDEEVLT DACWALSYLS DGTNDKIQAV IEAGVCPRLV ELLLHPSPSV 310 320 330 340 350 360 LIPALRTVGN IVTGDDAQTQ CIIDHQALPC LLSLLTQNLK KSIKKEACWT ISNITAGNKD 370 380 390 400 410 420 QIQAVINAGI IGPLVNLLQT AEFDIKKEAA WAISNATSGG SHDQIKYLVS EGCIKPLCDL 430 440 450 460 470 480 LICPDIRIVT VCLEGLENIL KVGETDKTLA AGDVNVFSQM IDEAEGLEKI ENLQSHDNNE 490 500 510 520 IYEKAVKILE AYWMDEEDDT MGATTVAAPQ GATFDFGQGG GAAQFK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a cDNA encoding an importin-alpha and down-regulation of the gene by light in rice leaves." Shoji K., Iwasaki T., Matsuki R., Miyao M., Yamamoto N. Gene 212:279-286(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, INDUCTION. Strain: cv. Nipponbare. Tissue: Callus and Leaf.
[2]	"Importin alpha-3, a novel variant of the small importin subunit evolutionarily conserved from hydrozoa to man." Fischer R. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Functional characterization of a plant importin alpha homologue. Nuclear localization signal (NLS)-selective binding and mediation of nuclear import of nls proteins in vitro." Jiang C.-J., Imamoto N., Matsuki R., Yoneda Y., Yamamoto N. J. Biol. Chem. 273:24083-24087(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Molecular cloning of a novel importin alpha homologue from rice, by which constitutive photomorphogenic 1 (COP1) nuclear localization signal (NLS)-protein is preferentially nuclear imported." Jiang C.-J., Shoji K., Matsuki R., Baba A., Inagaki N., Ban H., Iwasaki T., Imamoto N., Yoneda Y., Deng X.-W., Yamamoto N. J. Biol. Chem. 276:9322-9329(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION, INTERACTION WITH IMPORTIN BETA-1.
[6]	"Coat proteins of Rice tungro bacilliform virus and Mungbean yellow mosaic virus contain multiple nuclear-localization signals and interact with importin alpha." Guerra-Peraza O., Kirk D., Seltzer V., Veluthambi K., Schmit A.C., Hohn T., Herzog E. J. Gen. Virol. 86:1815-1826(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MUNGBEAN YELLOW MOSAIC VIRUS CAPSID PROTEIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB004660 mRNA. Translation: BAA31165.1.
AF005265 mRNA. Translation: AAQ13406.1.
AB004814 Genomic DNA. Translation: BAA31166.1.
AP000816 Genomic DNA. Translation: BAA87855.1.

RefSeq

NP_001042611.1. NM_001049146.1.

UniGene

Os.3405.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YNS	X-ray	2.10	A/B	73-526	[»]
4B8J	X-ray	2.00	A	1-526	[»]
4B8O	X-ray	2.08	A	73-526	[»]
4B8P	X-ray	2.30	A/B	73-526	[»]

ProteinModelPortal

Q71VM4.

SMR

Q71VM4. Positions 77-496.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q71VM4. 1 interaction.

MINT

MINT-6948326.

Proteomic databases

PRIDE

Q71VM4.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

LOC_Os01g14950.1; LOC_Os01g14950.1; LOC_Os01g14950.

GeneID

4327117.

KEGG

osa:4327117.

Organism-specific databases

Gramene

Q71VM4.

Phylogenomic databases

eggNOG

COG5064.

HOGENOM

HOG000167616.

OMA

MQTKTVI.

Gene expression databases

ArrayExpress

Q71VM4.

Family and domain databases

Gene3D

1.25.10.10. 1 hit.

InterPro

IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR002652. Importin-a_IBB.
IPR024931. Importing_su_alpha.
[Graphical view]

Pfam

PF00514. Arm. 8 hits.
PF01749. IBB. 1 hit.
[Graphical view]

PIRSF

PIRSF005673. Importin_alpha. 1 hit.

SMART

SM00185. ARM. 8 hits.
[Graphical view]

SUPFAM

SSF48371. ARM-type_fold. 1 hit.

PROSITE

PS50176. ARM_REPEAT. 4 hits.
PS51214. IBB. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

IMA1A_ORYSJ

Accession

Primary (citable) accession number: Q71VM4
Secondary accession number(s): O82783

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	April 26, 2005
Last modified:	April 3, 2013
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program