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Q71VM4

- IMA1A_ORYSJ

UniProt

Q71VM4 - IMA1A_ORYSJ

Protein

Importin subunit alpha-1a

Gene

Os01g0253300

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 2 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Functions in nuclear protein import. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Promotes docking of import substrates to the nuclear envelope.2 Publications

    GO - Molecular functioni

    1. protein transporter activity Source: InterPro

    GO - Biological processi

    1. defense response Source: EnsemblPlants/Gramene
    2. pollen sperm cell differentiation Source: EnsemblPlants/Gramene
    3. protein import into nucleus Source: InterPro
    4. viral process Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Importin subunit alpha-1a
    Gene namesi
    Ordered Locus Names:Os01g0253300, LOC_Os01g14950
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 1

    Organism-specific databases

    GrameneiQ71VM4.

    Subcellular locationi

    Cytoplasmperinuclear region 1 Publication

    GO - Cellular componenti

    1. cell wall Source: EnsemblPlants/Gramene
    2. cytosol Source: EnsemblPlants/Gramene
    3. nuclear envelope Source: EnsemblPlants/Gramene
    4. nucleolus Source: EnsemblPlants/Gramene
    5. perinuclear region of cytoplasm Source: UniProtKB-SubCell
    6. plasmodesma Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 526526Importin subunit alpha-1aPRO_0000120740Add
    BLAST

    Proteomic databases

    PRIDEiQ71VM4.

    Expressioni

    Tissue specificityi

    Highly expressed in callus, followed by root and etiolated leaf. Low expression in green leaf.2 Publications

    Inductioni

    In both etiolated and green leaves, down-regulated by light. In green leaf, increased by dark treatment.2 Publications

    Interactioni

    Subunit structurei

    Forms a complex with importin subunit beta-1. The whole complex, most stable and composed of importin alpha, importin beta and NLS substrate, is referred to as PTAC or pore targeting complex. Interacts with mungbean yellow mosaic virus capsid protein.2 Publications

    Protein-protein interaction databases

    IntActiQ71VM4. 2 interactions.
    MINTiMINT-6948326.

    Structurei

    Secondary structure

    1
    526
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi74 – 829
    Helixi86 – 10015
    Beta strandi103 – 1053
    Helixi108 – 1136
    Helixi117 – 1237
    Helixi130 – 14415
    Helixi148 – 1569
    Helixi159 – 1668
    Helixi172 – 18615
    Helixi190 – 1989
    Helixi202 – 2076
    Helixi215 – 22915
    Beta strandi231 – 2333
    Helixi237 – 2404
    Helixi243 – 2508
    Helixi256 – 26914
    Beta strandi271 – 2733
    Helixi274 – 2829
    Helixi286 – 2927
    Helixi298 – 31114
    Helixi316 – 3238
    Turni324 – 3263
    Helixi327 – 33610
    Helixi341 – 35515
    Helixi359 – 36810
    Helixi371 – 38010
    Helixi383 – 39917
    Helixi402 – 4109
    Helixi414 – 4196
    Helixi420 – 4223
    Helixi426 – 44924
    Helixi456 – 4638
    Helixi466 – 4738
    Helixi479 – 49214

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2YNSX-ray2.10A/B73-526[»]
    4B8JX-ray2.00A1-526[»]
    4B8OX-ray2.08A73-526[»]
    4B8PX-ray2.30A/B73-526[»]
    4BPLX-ray2.30A73-526[»]
    4BQKX-ray2.00A/B73-526[»]
    ProteinModelPortaliQ71VM4.
    SMRiQ71VM4. Positions 77-496.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 5858IBBPROSITE-ProRule annotationAdd
    BLAST
    Repeati105 – 14541ARM 1Add
    BLAST
    Repeati148 – 18740ARM 2Add
    BLAST
    Repeati190 – 23041ARM 3Add
    BLAST
    Repeati232 – 27140ARM 4Add
    BLAST
    Repeati274 – 31340ARM 5Add
    BLAST
    Repeati316 – 35641ARM 6Add
    BLAST
    Repeati359 – 39840ARM 7Add
    BLAST
    Repeati402 – 44140ARM 8Add
    BLAST

    Sequence similaritiesi

    Belongs to the importin alpha family.Curated
    Contains 8 ARM repeats.PROSITE-ProRule annotation
    Contains 1 IBB domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5064.
    HOGENOMiHOG000167616.
    OMAiMEKIFEC.

    Family and domain databases

    Gene3Di1.25.10.10. 1 hit.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR000225. Armadillo.
    IPR002652. Importin-a_IBB.
    IPR024931. Importing_su_alpha.
    [Graphical view]
    PfamiPF00514. Arm. 8 hits.
    PF01749. IBB. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005673. Importin_alpha. 1 hit.
    SMARTiSM00185. ARM. 8 hits.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    PROSITEiPS50176. ARM_REPEAT. 4 hits.
    PS51214. IBB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q71VM4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLRPSERVE VRRNRYKVAV DAEEGRRRRE DNMVEIRKSR REESLLKKRR    50
    EGLQAQAPVP ASAATGVDKK LESLPAMIGG VYSDDNNLQL EATTQFRKLL 100
    SIERSPPIEE VIQSGVVPRF VQFLTREDFP QLQFEAAWAL TNIASGTSEN 150
    TKVVIDHGAV PIFVKLLGSS SDDVREQAVW ALGNVAGDSP KCRDLVLANG 200
    ALLPLLAQLN EHTKLSMLRN ATWTLSNFCR GKPQPSFEQT RPALPALARL 250
    IHSNDEEVLT DACWALSYLS DGTNDKIQAV IEAGVCPRLV ELLLHPSPSV 300
    LIPALRTVGN IVTGDDAQTQ CIIDHQALPC LLSLLTQNLK KSIKKEACWT 350
    ISNITAGNKD QIQAVINAGI IGPLVNLLQT AEFDIKKEAA WAISNATSGG 400
    SHDQIKYLVS EGCIKPLCDL LICPDIRIVT VCLEGLENIL KVGETDKTLA 450
    AGDVNVFSQM IDEAEGLEKI ENLQSHDNNE IYEKAVKILE AYWMDEEDDT 500
    MGATTVAAPQ GATFDFGQGG GAAQFK 526
    Length:526
    Mass (Da):57,571
    Last modified:April 26, 2005 - v2
    Checksum:i7A7DA264968FC983
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti473 – 4742LQ → FE in AAQ13406. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB004660 mRNA. Translation: BAA31165.1.
    AF005265 mRNA. Translation: AAQ13406.1.
    AB004814 Genomic DNA. Translation: BAA31166.1.
    AP000816 Genomic DNA. Translation: BAA87855.1.
    RefSeqiNP_001042611.1. NM_001049146.1.
    UniGeneiOs.3405.

    Genome annotation databases

    EnsemblPlantsiOS01T0253300-01; OS01T0253300-01; OS01G0253300.
    OS01T0253300-02; OS01T0253300-02; OS01G0253300.
    GeneIDi4327117.
    KEGGiosa:4327117.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB004660 mRNA. Translation: BAA31165.1 .
    AF005265 mRNA. Translation: AAQ13406.1 .
    AB004814 Genomic DNA. Translation: BAA31166.1 .
    AP000816 Genomic DNA. Translation: BAA87855.1 .
    RefSeqi NP_001042611.1. NM_001049146.1.
    UniGenei Os.3405.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2YNS X-ray 2.10 A/B 73-526 [» ]
    4B8J X-ray 2.00 A 1-526 [» ]
    4B8O X-ray 2.08 A 73-526 [» ]
    4B8P X-ray 2.30 A/B 73-526 [» ]
    4BPL X-ray 2.30 A 73-526 [» ]
    4BQK X-ray 2.00 A/B 73-526 [» ]
    ProteinModelPortali Q71VM4.
    SMRi Q71VM4. Positions 77-496.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q71VM4. 2 interactions.
    MINTi MINT-6948326.

    Proteomic databases

    PRIDEi Q71VM4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS01T0253300-01 ; OS01T0253300-01 ; OS01G0253300 .
    OS01T0253300-02 ; OS01T0253300-02 ; OS01G0253300 .
    GeneIDi 4327117.
    KEGGi osa:4327117.

    Organism-specific databases

    Gramenei Q71VM4.

    Phylogenomic databases

    eggNOGi COG5064.
    HOGENOMi HOG000167616.
    OMAi MEKIFEC.

    Family and domain databases

    Gene3Di 1.25.10.10. 1 hit.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR000225. Armadillo.
    IPR002652. Importin-a_IBB.
    IPR024931. Importing_su_alpha.
    [Graphical view ]
    Pfami PF00514. Arm. 8 hits.
    PF01749. IBB. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005673. Importin_alpha. 1 hit.
    SMARTi SM00185. ARM. 8 hits.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    PROSITEi PS50176. ARM_REPEAT. 4 hits.
    PS51214. IBB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a cDNA encoding an importin-alpha and down-regulation of the gene by light in rice leaves."
      Shoji K., Iwasaki T., Matsuki R., Miyao M., Yamamoto N.
      Gene 212:279-286(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, INDUCTION.
      Strain: cv. Nipponbare.
      Tissue: Callus and Leaf.
    2. "Importin alpha-3, a novel variant of the small importin subunit evolutionarily conserved from hydrozoa to man."
      Fischer R.
      Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The genome sequence and structure of rice chromosome 1."
      Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
      , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
      Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "Functional characterization of a plant importin alpha homologue. Nuclear localization signal (NLS)-selective binding and mediation of nuclear import of nls proteins in vitro."
      Jiang C.-J., Imamoto N., Matsuki R., Yoneda Y., Yamamoto N.
      J. Biol. Chem. 273:24083-24087(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    5. "Molecular cloning of a novel importin alpha homologue from rice, by which constitutive photomorphogenic 1 (COP1) nuclear localization signal (NLS)-protein is preferentially nuclear imported."
      Jiang C.-J., Shoji K., Matsuki R., Baba A., Inagaki N., Ban H., Iwasaki T., Imamoto N., Yoneda Y., Deng X.-W., Yamamoto N.
      J. Biol. Chem. 276:9322-9329(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION, INTERACTION WITH IMPORTIN BETA-1.
    6. "Coat proteins of Rice tungro bacilliform virus and Mungbean yellow mosaic virus contain multiple nuclear-localization signals and interact with importin alpha."
      Guerra-Peraza O., Kirk D., Seltzer V., Veluthambi K., Schmit A.C., Hohn T., Herzog E.
      J. Gen. Virol. 86:1815-1826(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MUNGBEAN YELLOW MOSAIC VIRUS CAPSID PROTEIN.

    Entry informationi

    Entry nameiIMA1A_ORYSJ
    AccessioniPrimary (citable) accession number: Q71VM4
    Secondary accession number(s): O82783
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 65 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3