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Q71UI9 (H2AV_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histone H2A.V
Alternative name(s):
H2A.F/Z
Gene names
Name:H2AFV
Synonyms:H2AV
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length128 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division By similarity.

Subunit structure

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFV forms a heterodimer with H2B By similarity.

Subcellular location

Nucleus By similarity. Chromosome By similarity.

Post-translational modification

Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression By similarity.

Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis By similarity.

Sequence similarities

Belongs to the histone H2A family.

Mass spectrometry

Molecular mass is 13369.4 Da from positions 2 - 128. Determined by ESI. Monoisotopic, not modified. Ref.7

Sequence caution

The sequence BAD92238.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentChromosome
Nucleosome core
Nucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   LigandDNA-binding
   PTMAcetylation
Isopeptide bond
Ubl conjugation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processnucleosome assembly

Inferred from electronic annotation. Source: InterPro

   Cellular_componentnucleosome

Inferred from electronic annotation. Source: UniProtKB-KW

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q71UI9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q71UI9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     109-128: GVIPHIHKSLIGKKGQQKTA → EKRRCS
Note: No experimental confirmation available.
Isoform 3 (identifier: Q71UI9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     28-65: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q71UI9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: MAGGKAGKDSGKAKAKAVSRSQRAGLQ → M
Note: No experimental confirmation available.
Isoform 5 (identifier: Q71UI9-5)

The sequence of this isoform differs from the canonical sequence as follows:
     67-128: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 128128Histone H2A.V
PRO_0000239068

Amino acid modifications

Modified residue51N6-acetyllysine By similarity
Modified residue81N6-acetyllysine By similarity
Modified residue121N6-acetyllysine By similarity
Cross-link122Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Natural variations

Alternative sequence1 – 2727MAGGK…RAGLQ → M in isoform 4.
VSP_045232
Alternative sequence28 – 6538Missing in isoform 3.
VSP_044633
Alternative sequence67 – 12862Missing in isoform 5.
VSP_046783
Alternative sequence109 – 12820GVIPH…QQKTA → EKRRCS in isoform 2.
VSP_042855
Natural variant1251Q → R.
Corresponds to variant rs1802437 [ dbSNP | Ensembl ].
VAR_059312

Secondary structure

.................. 128
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 1F3C388F6854041C

FASTA12813,509
        10         20         30         40         50         60 
MAGGKAGKDS GKAKAKAVSR SQRAGLQFPV GRIHRHLKTR TTSHGRVGAT AAVYSAAILE 

        70         80         90        100        110        120 
YLTAEVLELA GNASKDLKVK RITPRHLQLA IRGDEELDSL IKATIAGGGV IPHIHKSLIG 


KKGQQKTA 

« Hide

Isoform 2 [UniParc].

Checksum: 4CF80C91A39397B4
Show »

FASTA11412,146
Isoform 3 [UniParc].

Checksum: 34DA01283B7A93F1
Show »

FASTA909,375
Isoform 4 [UniParc].

Checksum: 0E6071539B4139F9
Show »

FASTA10210,999
Isoform 5 [UniParc].

Checksum: 90DA3A9820A88CB0
Show »

FASTA666,892

References

« Hide 'large scale' references
[1]"Novel human member of the variant histone family."
Groitl P., Wolf H., Niller H.H.
Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[4]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3 AND 4).
Tissue: Kidney, Ovarian adenocarcinoma, Prostatic carcinoma, Skin and Uterus.
[7]"Precise characterization of human histones in the H2A gene family by top down mass spectrometry."
Boyne M.T. II, Pesavento J.J., Mizzen C.A., Kelleher N.L.
J. Proteome Res. 5:248-253(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF081192 mRNA. Translation: AAC31938.1.
AK023973 mRNA. Translation: BAG51243.1.
AB209001 mRNA. Translation: BAD92238.1. Different initiation.
AC004854 Genomic DNA. Translation: AAS00365.1.
CH471128 Genomic DNA. Translation: EAW61075.1.
CH471128 Genomic DNA. Translation: EAW61077.1.
BC000098 mRNA. Translation: AAH00098.1.
BC004274 mRNA. Translation: AAH04274.3.
BC014885 mRNA. Translation: AAH14885.1.
BC070169 mRNA. Translation: AAH70169.1.
BE742590 mRNA. No translation available.
BU543626 mRNA. No translation available.
RefSeqNP_036544.1. NM_012412.4.
NP_619541.1. NM_138635.3.
NP_958844.1. NM_201436.2.
NP_958924.1. NM_201516.2.
NP_958925.1. NM_201517.2.
UniGeneHs.488189.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3WAAX-ray3.20C/G1-128[»]
ProteinModelPortalQ71UI9.
SMRQ71UI9. Positions 17-123.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125150. 28 interactions.
IntActQ71UI9. 3 interactions.
MINTMINT-4828370.
STRING9606.ENSP00000308405.

PTM databases

PhosphoSiteQ71UI9.

Polymorphism databases

DMDM74749787.

Proteomic databases

PaxDbQ71UI9.
PRIDEQ71UI9.

Protocols and materials databases

DNASU94239.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000222690; ENSP00000222690; ENSG00000105968. [Q71UI9-2]
ENST00000308153; ENSP00000308405; ENSG00000105968. [Q71UI9-1]
ENST00000349299; ENSP00000342714; ENSG00000105968. [Q71UI9-3]
ENST00000350771; ENSP00000340708; ENSG00000105968. [Q71UI9-4]
ENST00000381124; ENSP00000370516; ENSG00000105968. [Q71UI9-5]
GeneID94239.
KEGGhsa:94239.
UCSCuc003tlz.2. human. [Q71UI9-2]
uc003tma.2. human. [Q71UI9-1]
uc003tmb.2. human.

Organism-specific databases

CTD94239.
GeneCardsGC07M044833.
HGNCHGNC:20664. H2AFV.
HPAHPA045242.
neXtProtNX_Q71UI9.
PharmGKBPA134895050.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5262.
HOGENOMHOG000234652.
HOVERGENHBG009342.
InParanoidQ71UI9.
KOK11251.
OMAGNASKDM.
PhylomeDBQ71UI9.
TreeFamTF354232.

Gene expression databases

ArrayExpressQ71UI9.
BgeeQ71UI9.
CleanExHS_H2AFV.
GenevestigatorQ71UI9.

Family and domain databases

Gene3D1.10.20.10. 1 hit.
InterProIPR009072. Histone-fold.
IPR007125. Histone_core_D.
IPR002119. Histone_H2A.
[Graphical view]
PfamPF00125. Histone. 1 hit.
[Graphical view]
PRINTSPR00620. HISTONEH2A.
SMARTSM00414. H2A. 1 hit.
[Graphical view]
SUPFAMSSF47113. SSF47113. 1 hit.
PROSITEPS00046. HISTONE_H2A. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSH2AFV. human.
GeneWikiH2AFV.
GenomeRNAi94239.
NextBio78481.
PROQ71UI9.

Entry information

Entry nameH2AV_HUMAN
AccessionPrimary (citable) accession number: Q71UI9
Secondary accession number(s): A6NFA8 expand/collapse secondary AC list , A6NKY0, A6NN01, A8MQC5, Q59GV8, Q6PK98
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: January 23, 2007
Last modified: March 19, 2014
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM