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Protein

NEDD8

Gene

Nedd8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • protein localization Source: Ensembl
  • protein neddylation Source: RGD
  • regulation of transcription by RNA polymerase II Source: Ensembl
  • response to organic cyclic compound Source: RGD

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-RNO-2173789 TGF-beta receptor signaling activates SMADs
R-RNO-5689603 UCH proteinases
R-RNO-8856825 Cargo recognition for clathrin-mediated endocytosis
R-RNO-8951664 Neddylation
R-RNO-917937 Iron uptake and transport

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8
Alternative name(s):
Neddylin
Ubiquitin-like protein NEDD8
Gene namesi
Name:Nedd8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi3158 Nedd8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000427731 – 76NEDD8Add BLAST76
PropeptideiPRO_000004277477 – 81By similarity5

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48N6-acetyllysineBy similarity1
Cross-linki76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

Post-translational modificationi

Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond

Proteomic databases

PaxDbiQ71UE8
PRIDEiQ71UE8

PTM databases

iPTMnetiQ71UE8
PhosphoSitePlusiQ71UE8

Expressioni

Gene expression databases

BgeeiENSRNOG00000019895
ExpressionAtlasiQ71UE8 baseline and differential
GenevisibleiQ71UE8 RN

Interactioni

Subunit structurei

Directly interacts with NUB1 and AHR. Covalently attached to cullins and p53 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei8Interaction with UBE1CBy similarity1
Sitei44Interaction with UBE1CBy similarity1

GO - Molecular functioni

Protein-protein interaction databases

BioGridi247523, 2 interactors
DIPiDIP-60385N
IntActiQ71UE8, 1 interactor
STRINGi10116.ENSRNOP00000061284

Structurei

3D structure databases

ProteinModelPortaliQ71UE8
SMRiQ71UE8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 72Interaction with UBE1CBy similarity3

Sequence similaritiesi

Belongs to the ubiquitin family.Curated

Phylogenomic databases

eggNOGiKOG0005 Eukaryota
COG5272 LUCA
GeneTreeiENSGT00900000141147
HOGENOMiHOG000233942
HOVERGENiHBG000057
InParanoidiQ71UE8
KOiK12158
PhylomeDBiQ71UE8
TreeFamiTF300072

Family and domain databases

CDDicd01806 Nedd8, 1 hit
InterProiView protein in InterPro
IPR038738 Nedd8-like
IPR019956 Ubiquitin
IPR029071 Ubiquitin-like_domsf
IPR019954 Ubiquitin_CS
IPR000626 Ubiquitin_dom
PfamiView protein in Pfam
PF00240 ubiquitin, 1 hit
PRINTSiPR00348 UBIQUITIN
SMARTiView protein in SMART
SM00213 UBQ, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS00299 UBIQUITIN_1, 1 hit
PS50053 UBIQUITIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q71UE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIKVKTLTG KEIEIDIEPT DKVERIKERV EEKEGIPPQQ QRLIYSGKQM
60 70 80
NDEKTAADYK ILGGSVLHLV LALRGGGGLG Q
Length:81
Mass (Da):8,972
Last modified:July 5, 2004 - v1
Checksum:iDC339102BE4725D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095740 mRNA Translation: AAC64189.1
BC084728 mRNA Translation: AAH84728.1
RefSeqiNP_620233.1, NM_138878.2
UniGeneiRn.216415

Genome annotation databases

EnsembliENSRNOT00000064916; ENSRNOP00000061284; ENSRNOG00000019895
GeneIDi25490
KEGGirno:25490
UCSCiRGD:3158 rat

Similar proteinsi

Entry informationi

Entry nameiNEDD8_RAT
AccessioniPrimary (citable) accession number: Q71UE8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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