Q71UE8 (NEDD8_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NEDD8

Alternative name(s):
Neddylin
Ubiquitin-like protein NEDD8

Gene names

Name:

Nedd8

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	81 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins By similarity.
Subunit structure	Directly interacts with NUB1 and AHR. Covalently attached to cullins and p53 By similarity.
Subcellular location	Nucleus. Note: Mainly nuclear By similarity.
Post-translational modification	Cleavage of precursor form by UCHL3 or SENP8 is necessary for function By similarity.
Sequence similarities	Belongs to the ubiquitin family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
PTM	Acetylation Isopeptide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	protein neddylation Inferred from direct assay. Source: RGD response to organic cyclic compound Inferred from direct assay. Source: RGD
Cellular component	nucleus Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 76	76	NEDD8		PRO_0000042773
	Propeptide	77 – 81	5	By similarity		PRO_0000042774
Regions
	Region	70 – 72	3	Interaction with UBE1C By similarity
Sites
	Site	8	1	Interaction with UBE1C By similarity
	Site	44	1	Interaction with UBE1C By similarity
Amino acid modifications
	Modified residue	48	1	N6-acetyllysine By similarity
	Cross-link	76		Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q71UE8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: DC339102BE4725D2	FASTA	81	8,972
	10 20 30 40 50 60 MLIKVKTLTG KEIEIDIEPT DKVERIKERV EEKEGIPPQQ QRLIYSGKQM NDEKTAADYK 70 80 ILGGSVLHLV LALRGGGGLG Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a novel ubiquitin-like protein from the diabetic kidney." Page R.A. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: GK. Tissue: Kidney.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	Lubec G., Diao W. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 61-74, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF095740 mRNA. Translation: AAC64189.1. BC084728 mRNA. Translation: AAH84728.1.
IPI	IPI00196202.
RefSeq	NP_620233.1. NM_138878.2.
UniGene	Rn.216415.
3D structure databases
ProteinModelPortal	Q71UE8.
SMR	Q71UE8. Positions 1-76.
ModBase	Search...
Protein-protein interaction databases
STRING	Q71UE8.
PTM databases
PhosphoSite	Q71UE8.
Proteomic databases
PRIDE	Q71UE8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000064916; ENSRNOP00000061284; ENSRNOG00000019895.
GeneID	25490.
KEGG	rno:25490.
UCSC	NM_138878. rat.
Organism-specific databases
CTD	4738.
RGD	3158. Nedd8.
Phylogenomic databases
eggNOG	maNOG23639.
GeneTree	ENSGT00560000077356.
HOVERGEN	HBG000057.
InParanoid	Q71UE8.
OrthoDB	EOG4N04GQ.
Gene expression databases
ArrayExpress	Q71UE8.
Genevestigator	Q71UE8.
GermOnline	ENSRNOG00000019895. Rattus norvegicus.
Family and domain databases
InterPro	IPR000626. Ubiquitin. IPR019954. Ubiquitin_CS. IPR019956. Ubiquitin_subgr. IPR019955. Ubiquitin_supergroup. [Graphical view]
KO	K12158.
Pfam	PF00240. ubiquitin. 1 hit. [Graphical view]
PRINTS	PR00348. UBIQUITIN.
SMART	SM00213. UBQ. 1 hit. [Graphical view]
PROSITE	PS00299. UBIQUITIN_1. 1 hit. PS50053. UBIQUITIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	606857.

Entry information

Entry name

NEDD8_RAT

Accession

Primary (citable) accession number: Q71UE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program