Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NEDD8

Gene

Nedd8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins (By similarity).By similarity

GO - Biological processi

  • protein localization Source: Ensembl
  • protein neddylation Source: RGD
  • regulation of transcription from RNA polymerase II promoter Source: Ensembl
  • response to organic cyclic compound Source: RGD
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-RNO-2173789. TGF-beta receptor signaling activates SMADs.

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8
Alternative name(s):
Neddylin
Ubiquitin-like protein NEDD8
Gene namesi
Name:Nedd8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi3158. Nedd8.

Subcellular locationi

  • Nucleus

  • Note: Mainly nuclear.By similarity

GO - Cellular componenti

  • extracellular exosome Source: Ensembl
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7676NEDD8PRO_0000042773Add
BLAST
Propeptidei77 – 815By similarityPRO_0000042774

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481N6-acetyllysineBy similarity
Cross-linki76 – 76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

Post-translational modificationi

Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond

Proteomic databases

PaxDbiQ71UE8.
PRIDEiQ71UE8.

PTM databases

iPTMnetiQ71UE8.
PhosphoSiteiQ71UE8.

Expressioni

Gene expression databases

BgeeiENSRNOG00000019895.
ExpressionAtlasiQ71UE8. baseline and differential.
GenevisibleiQ71UE8. RN.

Interactioni

Subunit structurei

Directly interacts with NUB1 and AHR. Covalently attached to cullins and p53 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei8 – 81Interaction with UBE1CBy similarity
Sitei44 – 441Interaction with UBE1CBy similarity

Protein-protein interaction databases

BioGridi247523. 2 interactions.
DIPiDIP-60385N.
STRINGi10116.ENSRNOP00000061284.

Structurei

3D structure databases

ProteinModelPortaliQ71UE8.
SMRiQ71UE8. Positions 1-76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni70 – 723Interaction with UBE1CBy similarity

Sequence similaritiesi

Belongs to the ubiquitin family.Curated

Phylogenomic databases

eggNOGiKOG0005. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00840000129851.
HOGENOMiHOG000233942.
HOVERGENiHBG000057.
InParanoidiQ71UE8.
KOiK12158.
PhylomeDBiQ71UE8.
TreeFamiTF300072.

Family and domain databases

InterProiIPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
[Graphical view]
PRINTSiPR00348. UBIQUITIN.
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q71UE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIKVKTLTG KEIEIDIEPT DKVERIKERV EEKEGIPPQQ QRLIYSGKQM
60 70 80
NDEKTAADYK ILGGSVLHLV LALRGGGGLG Q
Length:81
Mass (Da):8,972
Last modified:July 5, 2004 - v1
Checksum:iDC339102BE4725D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095740 mRNA. Translation: AAC64189.1.
BC084728 mRNA. Translation: AAH84728.1.
RefSeqiNP_620233.1. NM_138878.2.
UniGeneiRn.216415.

Genome annotation databases

EnsembliENSRNOT00000064916; ENSRNOP00000061284; ENSRNOG00000019895.
GeneIDi25490.
KEGGirno:25490.
UCSCiRGD:3158. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095740 mRNA. Translation: AAC64189.1.
BC084728 mRNA. Translation: AAH84728.1.
RefSeqiNP_620233.1. NM_138878.2.
UniGeneiRn.216415.

3D structure databases

ProteinModelPortaliQ71UE8.
SMRiQ71UE8. Positions 1-76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247523. 2 interactions.
DIPiDIP-60385N.
STRINGi10116.ENSRNOP00000061284.

PTM databases

iPTMnetiQ71UE8.
PhosphoSiteiQ71UE8.

Proteomic databases

PaxDbiQ71UE8.
PRIDEiQ71UE8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000064916; ENSRNOP00000061284; ENSRNOG00000019895.
GeneIDi25490.
KEGGirno:25490.
UCSCiRGD:3158. rat.

Organism-specific databases

CTDi4738.
RGDi3158. Nedd8.

Phylogenomic databases

eggNOGiKOG0005. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00840000129851.
HOGENOMiHOG000233942.
HOVERGENiHBG000057.
InParanoidiQ71UE8.
KOiK12158.
PhylomeDBiQ71UE8.
TreeFamiTF300072.

Enzyme and pathway databases

ReactomeiR-RNO-2173789. TGF-beta receptor signaling activates SMADs.

Miscellaneous databases

PROiQ71UE8.

Gene expression databases

BgeeiENSRNOG00000019895.
ExpressionAtlasiQ71UE8. baseline and differential.
GenevisibleiQ71UE8. RN.

Family and domain databases

InterProiIPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
[Graphical view]
PRINTSiPR00348. UBIQUITIN.
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEDD8_RAT
AccessioniPrimary (citable) accession number: Q71UE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.