Q71SY5 (MED25_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mediator of RNA polymerase II transcription subunit 25

Alternative name(s):
Activator interaction domain-containing protein 1
Activator-recruited cofactor 92 kDa component
Short name=ARC92
Mediator complex subunit 25
p78

Gene names

Name:	MED25
Synonyms:	ACID1, ARC92, PTOV2
ORF Names:	TCBAP0758

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	747 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription. Ref.2 Ref.3 Ref.12
Subunit structure	Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with CREBBP. Interacts with ESR1, GR, RARA, RXRA and THRB in a ligand-dependent fashion. Binds the Herpes simplex virus activator VP16. Ref.2 Ref.3 Ref.10 Ref.11 Ref.12
Subcellular location	Nucleus Ref.2.
Tissue specificity	Ubiquitously expressed. Highest levels in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen. Ref.2 Ref.3
Involvement in disease	Charcot-Marie-Tooth disease 2B2 (CMT2B2) [MIM:605589]: A recessive axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.14
Sequence similarities	Belongs to the Mediator complex subunit 25 family.
Sequence caution	The sequence AAG15589.1 differs from that shown. Reason: Erroneous initiation. The sequence CAB66680.1 differs from that shown. Reason: Frameshift at position 567.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Disease	Charcot-Marie-Tooth disease Disease mutation Neuropathy
Molecular function	Activator
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of fibroblast proliferation Inferred from mutant phenotype Ref.12. Source: UniProtKB negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype Ref.12. Source: UniProtKB positive regulation of cell cycle arrest Inferred from mutant phenotype Ref.12. Source: UniProtKB positive regulation of chromatin binding Inferred from mutant phenotype Ref.12. Source: UniProtKB positive regulation of mediator complex assembly Inferred from mutant phenotype Ref.12. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.12. Source: UniProtKB transcription initiation from RNA polymerase II promoter Traceable author statement. Source: Reactome
Cellular_component	nucleoplasm Traceable author statement. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q71SY5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q71SY5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     671-747: QPPGAPALLP...MEDDILMDLI → PSPGPHNFPL...ESPTPNKVPF

Isoform 3 (identifier: Q71SY5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     701-747: AQSWPAQLPP...MEDDILMDLI → PATRGSCAAA...VERIQPETPL

Note: No experimental confirmation available.

Isoform 4 (identifier: Q71SY5-4)

The sequence of this isoform differs from the canonical sequence as follows:
     716-747: GQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI → AAKRKREGEG...IRARPIWPDP

Note: No experimental confirmation available.

Isoform 5 (identifier: Q71SY5-5)

The sequence of this isoform differs from the canonical sequence as follows:
     303-319: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 747

747

Mediator of RNA polymerase II transcription subunit 25

PRO_0000304952

Regions

Region

1 – 226

226

Interaction with the Mediator complex

Region

389 – 543

155

Interaction with VP16

Region

395 – 545

151

Interaction with CREBBP

Region

564 – 653

90

Interaction with RARA

Region

640 – 707

68

Interaction with RARA

Motif

646 – 650

5

LXXLL motif

Compositional bias

200 – 381

182

Pro-rich

Compositional bias

565 – 735

171

Pro-rich

Natural variations

Alternative sequence

303 – 319

17

Missing in isoform 5.

VSP_028143

Alternative sequence

671 – 747

77

QPPGA…LMDLI → PSPGPHNFPLGLHCQVRGPG GGQRSGLSVPAAPGLEHQDQ VLLGSKDIGSKNEGSPSRAC SPASWRTTSSWISSESPTPN KVPF in isoform 2.

VSP_028144

Alternative sequence

701 – 747

47

AQSWP…LMDLI → PATRGSCAAASAAPGPGAAP VGAPTPASTTCPVLARTTSP SGSTASGKTKERRRGPCVSR KMGASLFLCGSEEHAYVFNM QTNRVCVERIQPETPL in isoform 3.

VSP_028145

Alternative sequence

716 – 747

32

GQMLL…LMDLI → AAKRKREGEGRVFREKWERA YFFVEVKSMPMCLICKQIVS VLKEYNLKRHYESKHSKSYD QYTEQTRRIRARPIWPDP in isoform 4.

VSP_028146

Natural variant

335

1

A → V in CMT2B2. Ref.14

VAR_063521

Experimental info

Mutagenesis

646

1

L → A: Abrogates interaction with RARA. Ref.12

Mutagenesis

649 – 650

2

LL → AA: Abrogates interaction with RARA.

Sequence conflict

20

1

F → S in AAM20739. Ref.1

Sequence conflict

236

1

P → L in CAE84581. Ref.2

Sequence conflict

236

1

P → L in CAB66680. Ref.4

Sequence conflict

268

1

V → I in AAM20739. Ref.1

Sequence conflict

514

1

L → M in AAS45401. Ref.3

Sequence conflict

536

1

G → D in AAS45401. Ref.3

Sequence conflict

703

1

S → F in AAM20739. Ref.1

Secondary structure

1

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747

Helix Strand Turn

Details...

Beta strand

396 – 409

14

Beta strand

414 – 417

4

Beta strand

424 – 434

11

Helix

441 – 443

3

Beta strand

446 – 454

9

Helix

455 – 461

7

Helix

462 – 465

4

Beta strand

466 – 475

10

Beta strand

476 – 478

3

Helix

480 – 493

14

Beta strand

494 – 499

6

Beta strand

502 – 504

3

Beta strand

510 – 515

6

Turn

517 – 519

3

Beta strand

521 – 529

9

Helix

530 – 545

16

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 2, 2007. Version 2. Checksum: 6FE13FB45786402D	FASTA	747	78,171
	10 20 30 40 50 60 MVPGSEGPAR AGSVVADVVF VIEGTANLGP YFEGLRKHYL LPAIEYFNGG PPAETDFGGD 70 80 90 100 110 120 YGGTQYSLVV FNTVDCAPES YVQCHAPTSS AYEFVTWLDG IKFMGGGGES CSLIAEGLST 130 140 150 160 170 180 ALQLFDDFKK MREQIGQTHR VCLLICNSPP YLLPAVESTT YSGCTTENLV QQIGERGIHF 190 200 210 220 230 240 SIVSPRKLPA LRLLFEKAAP PALLEPLQPP TDVSQDPRHM VLVRGLVLPV GGGSAPGPLQ 250 260 270 280 290 300 SKQPVPLPPA APSGATLSAA PQQPLPPVPP QYQVPGNLSA AQVAAQNAVE AAKNQKAGLG 310 320 330 340 350 360 PRFSPITPLQ QAAPGVGPPF SQAPAPQLPP GPPGAPKPPP ASQPSLVSTV APGSGLAPTA 370 380 390 400 410 420 QPGAPSMAGT VAPGGVSGPS PAQLGAPALG GQQSVSNKLL AWSGVLEWQE KPKPASVDAN 430 440 450 460 470 480 TKLTRSLPCQ VYVNHGENLK TEQWPQKLIM QLIPQQLLTT LGPLFRNSRM VQFHFTNKDL 490 500 510 520 530 540 ESLKGLYRIM GNGFAGCVHF PHTAPCEVRV LMLLYSSKKK IFMGLIPYDQ SGFVNGIRQV 550 560 570 580 590 600 ITNHKQVQQQ KLEQQQRGMG GQQAPPGLGP ILEDQARPSQ NLLQLRPPQP QPQGTVGASG 610 620 630 640 650 660 ATGQPQPQGT AQPPPGAPQG PPGAASGPPP PGPILRPQNP GANPQLRSLL LNPPPPQTGV 670 680 690 700 710 720 PPPQASLHHL QPPGAPALLP PPHQGLGQPQ LGPPLLHPPP AQSWPAQLPP RAPLPGQMLL 730 740 SGGPRGPVPQ PGLQPSVMED DILMDLI
	Isoform 2 [UniParc]. Checksum: 0E8022B21685A1EE	FASTA	754	78,882
	10 20 30 40 50 60 MVPGSEGPAR AGSVVADVVF VIEGTANLGP YFEGLRKHYL LPAIEYFNGG PPAETDFGGD 70 80 90 100 110 120 YGGTQYSLVV FNTVDCAPES YVQCHAPTSS AYEFVTWLDG IKFMGGGGES CSLIAEGLST 130 140 150 160 170 180 ALQLFDDFKK MREQIGQTHR VCLLICNSPP YLLPAVESTT YSGCTTENLV QQIGERGIHF 190 200 210 220 230 240 SIVSPRKLPA LRLLFEKAAP PALLEPLQPP TDVSQDPRHM VLVRGLVLPV GGGSAPGPLQ 250 260 270 280 290 300 SKQPVPLPPA APSGATLSAA PQQPLPPVPP QYQVPGNLSA AQVAAQNAVE AAKNQKAGLG 310 320 330 340 350 360 PRFSPITPLQ QAAPGVGPPF SQAPAPQLPP GPPGAPKPPP ASQPSLVSTV APGSGLAPTA 370 380 390 400 410 420 QPGAPSMAGT VAPGGVSGPS PAQLGAPALG GQQSVSNKLL AWSGVLEWQE KPKPASVDAN 430 440 450 460 470 480 TKLTRSLPCQ VYVNHGENLK TEQWPQKLIM QLIPQQLLTT LGPLFRNSRM VQFHFTNKDL 490 500 510 520 530 540 ESLKGLYRIM GNGFAGCVHF PHTAPCEVRV LMLLYSSKKK IFMGLIPYDQ SGFVNGIRQV 550 560 570 580 590 600 ITNHKQVQQQ KLEQQQRGMG GQQAPPGLGP ILEDQARPSQ NLLQLRPPQP QPQGTVGASG 610 620 630 640 650 660 ATGQPQPQGT AQPPPGAPQG PPGAASGPPP PGPILRPQNP GANPQLRSLL LNPPPPQTGV 670 680 690 700 710 720 PPPQASLHHL PSPGPHNFPL GLHCQVRGPG GGQRSGLSVP AAPGLEHQDQ VLLGSKDIGS 730 740 750 KNEGSPSRAC SPASWRTTSS WISSESPTPN KVPF
	Isoform 3 [UniParc]. Checksum: 930FD1BDBEED7BA1	FASTA	796	82,972
	10 20 30 40 50 60 MVPGSEGPAR AGSVVADVVF VIEGTANLGP YFEGLRKHYL LPAIEYFNGG PPAETDFGGD 70 80 90 100 110 120 YGGTQYSLVV FNTVDCAPES YVQCHAPTSS AYEFVTWLDG IKFMGGGGES CSLIAEGLST 130 140 150 160 170 180 ALQLFDDFKK MREQIGQTHR VCLLICNSPP YLLPAVESTT YSGCTTENLV QQIGERGIHF 190 200 210 220 230 240 SIVSPRKLPA LRLLFEKAAP PALLEPLQPP TDVSQDPRHM VLVRGLVLPV GGGSAPGPLQ 250 260 270 280 290 300 SKQPVPLPPA APSGATLSAA PQQPLPPVPP QYQVPGNLSA AQVAAQNAVE AAKNQKAGLG 310 320 330 340 350 360 PRFSPITPLQ QAAPGVGPPF SQAPAPQLPP GPPGAPKPPP ASQPSLVSTV APGSGLAPTA 370 380 390 400 410 420 QPGAPSMAGT VAPGGVSGPS PAQLGAPALG GQQSVSNKLL AWSGVLEWQE KPKPASVDAN 430 440 450 460 470 480 TKLTRSLPCQ VYVNHGENLK TEQWPQKLIM QLIPQQLLTT LGPLFRNSRM VQFHFTNKDL 490 500 510 520 530 540 ESLKGLYRIM GNGFAGCVHF PHTAPCEVRV LMLLYSSKKK IFMGLIPYDQ SGFVNGIRQV 550 560 570 580 590 600 ITNHKQVQQQ KLEQQQRGMG GQQAPPGLGP ILEDQARPSQ NLLQLRPPQP QPQGTVGASG 610 620 630 640 650 660 ATGQPQPQGT AQPPPGAPQG PPGAASGPPP PGPILRPQNP GANPQLRSLL LNPPPPQTGV 670 680 690 700 710 720 PPPQASLHHL QPPGAPALLP PPHQGLGQPQ LGPPLLHPPP PATRGSCAAA SAAPGPGAAP 730 740 750 760 770 780 VGAPTPASTT CPVLARTTSP SGSTASGKTK ERRRGPCVSR KMGASLFLCG SEEHAYVFNM 790 QTNRVCVERI QPETPL
	Isoform 4 [UniParc]. Checksum: 4F9597470EB0E517	FASTA	793	84,389
	10 20 30 40 50 60 MVPGSEGPAR AGSVVADVVF VIEGTANLGP YFEGLRKHYL LPAIEYFNGG PPAETDFGGD 70 80 90 100 110 120 YGGTQYSLVV FNTVDCAPES YVQCHAPTSS AYEFVTWLDG IKFMGGGGES CSLIAEGLST 130 140 150 160 170 180 ALQLFDDFKK MREQIGQTHR VCLLICNSPP YLLPAVESTT YSGCTTENLV QQIGERGIHF 190 200 210 220 230 240 SIVSPRKLPA LRLLFEKAAP PALLEPLQPP TDVSQDPRHM VLVRGLVLPV GGGSAPGPLQ 250 260 270 280 290 300 SKQPVPLPPA APSGATLSAA PQQPLPPVPP QYQVPGNLSA AQVAAQNAVE AAKNQKAGLG 310 320 330 340 350 360 PRFSPITPLQ QAAPGVGPPF SQAPAPQLPP GPPGAPKPPP ASQPSLVSTV APGSGLAPTA 370 380 390 400 410 420 QPGAPSMAGT VAPGGVSGPS PAQLGAPALG GQQSVSNKLL AWSGVLEWQE KPKPASVDAN 430 440 450 460 470 480 TKLTRSLPCQ VYVNHGENLK TEQWPQKLIM QLIPQQLLTT LGPLFRNSRM VQFHFTNKDL 490 500 510 520 530 540 ESLKGLYRIM GNGFAGCVHF PHTAPCEVRV LMLLYSSKKK IFMGLIPYDQ SGFVNGIRQV 550 560 570 580 590 600 ITNHKQVQQQ KLEQQQRGMG GQQAPPGLGP ILEDQARPSQ NLLQLRPPQP QPQGTVGASG 610 620 630 640 650 660 ATGQPQPQGT AQPPPGAPQG PPGAASGPPP PGPILRPQNP GANPQLRSLL LNPPPPQTGV 670 680 690 700 710 720 PPPQASLHHL QPPGAPALLP PPHQGLGQPQ LGPPLLHPPP AQSWPAQLPP RAPLPAAKRK 730 740 750 760 770 780 REGEGRVFRE KWERAYFFVE VKSMPMCLIC KQIVSVLKEY NLKRHYESKH SKSYDQYTEQ 790 TRRIRARPIW PDP
	Isoform 5 [UniParc]. Checksum: 70D41D4F28B8A709	FASTA	730	76,512
	10 20 30 40 50 60 MVPGSEGPAR AGSVVADVVF VIEGTANLGP YFEGLRKHYL LPAIEYFNGG PPAETDFGGD 70 80 90 100 110 120 YGGTQYSLVV FNTVDCAPES YVQCHAPTSS AYEFVTWLDG IKFMGGGGES CSLIAEGLST 130 140 150 160 170 180 ALQLFDDFKK MREQIGQTHR VCLLICNSPP YLLPAVESTT YSGCTTENLV QQIGERGIHF 190 200 210 220 230 240 SIVSPRKLPA LRLLFEKAAP PALLEPLQPP TDVSQDPRHM VLVRGLVLPV GGGSAPGPLQ 250 260 270 280 290 300 SKQPVPLPPA APSGATLSAA PQQPLPPVPP QYQVPGNLSA AQVAAQNAVE AAKNQKAGLG 310 320 330 340 350 360 PRFSQAPAPQ LPPGPPGAPK PPPASQPSLV STVAPGSGLA PTAQPGAPSM AGTVAPGGVS 370 380 390 400 410 420 GPSPAQLGAP ALGGQQSVSN KLLAWSGVLE WQEKPKPASV DANTKLTRSL PCQVYVNHGE 430 440 450 460 470 480 NLKTEQWPQK LIMQLIPQQL LTTLGPLFRN SRMVQFHFTN KDLESLKGLY RIMGNGFAGC 490 500 510 520 530 540 VHFPHTAPCE VRVLMLLYSS KKKIFMGLIP YDQSGFVNGI RQVITNHKQV QQQKLEQQQR 550 560 570 580 590 600 GMGGQQAPPG LGPILEDQAR PSQNLLQLRP PQPQPQGTVG ASGATGQPQP QGTAQPPPGA 610 620 630 640 650 660 PQGPPGAASG PPPPGPILRP QNPGANPQLR SLLLNPPPPQ TGVPPPQASL HHLQPPGAPA 670 680 690 700 710 720 LLPPPHQGLG QPQLGPPLLH PPPAQSWPAQ LPPRAPLPGQ MLLSGGPRGP VPQPGLQPSV 730 MEDDILMDLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A prostate-derived cDNA that is mapped to human chromosome 19 encodes a novel protein." Wang C., McCarty I.M., Balazs L., Li Y., Steiner M.S. Biochem. Biophys. Res. Commun. 296:281-287(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"A novel docking site on Mediator is critical for activation by VP16 in mammalian cells." Mittler G., Stuehler T., Santolin L., Uhlmann T., Kremmer E., Lottspeich F., Berti L., Meisterernst M. EMBO J. 22:6494-6504(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PARTIAL PROTEIN SEQUENCE, FUNCTION, IDENTIFICATION IN THE MEDIATOR COMPLEX, INTERACTION WITH VP16, ASSOCIATION WITH PROMOTER REGIONS, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Testis.
[3]	"The activator-recruited cofactor/Mediator coactivator subunit ARC92 is a functionally important target of the VP16 transcriptional activator." Yang F., DeBeaumont R., Zhou S., Naeaer A.M. Proc. Natl. Acad. Sci. U.S.A. 101:2339-2344(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), PROTEIN SEQUENCE OF 1-11; 197-207; 241-254; 398-446 AND 520-530, FUNCTION, IDENTIFICATION IN THE MEDIATOR COMPLEX, INTERACTION WITH VP16, TISSUE SPECIFICITY.
[4]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 159-747 (ISOFORM 5). Tissue: Eye and Lung.
[9]	"Pediatric leukemia cDNA sequencing project." Villalon D.K., Luna R.A., Margolin J.K., Tsang Y.T.M., Hale S.M., Mei G., Bouck J., Gibbs R.A. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 178-747 (ISOFORM 4). Tissue: Leukocyte.
[10]	"A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology." Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C. Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
[11]	"MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription." Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G. Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[12]	"MED25 is distinct from TRAP220/MED1 in cooperating with CBP for retinoid receptor activation." Lee H.-K., Park U.-H., Kim E.-J., Um S.-J. EMBO J. 26:3545-3557(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ASSOCIATION WITH PROMOTER REGIONS, INTERACTION WITH CREBBP; ESR1; GR; MED1; MED6; RARA; RXRA AND THRB, MUTAGENESIS OF LEU-646 AND 649-LEU-LEU-650.
[13]	"NMR structure of the human Mediator MED25 ACID domain." Bontems F., Verger A., Dewitte F., Lens Z., Baert J.L., Ferreira E., Launoit Y.D., Sizun C., Guittet E., Villeret V., Monte D. J. Struct. Biol. 174:245-251(2011) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 391-548.
[14]	"Identification of the variant Ala335Val of MED25 as responsible for CMT2B2: molecular data, functional studies of the SH3 recognition motif and correlation between wild-type MED25 and PMP22 RNA levels in CMT1A animal models." Leal A., Huehne K., Bauer F., Sticht H., Berger P., Suter U., Morera B., Del Valle G., Lupski J.R., Ekici A., Pasutto F., Endele S., Barrantes R., Berghoff C., Berghoff M., Neundoerfer B., Heuss D., Dorn T. , Young P., Santolin L., Uhlmann T., Meisterernst M., Sereda M.W., Sereda M., Stassart R.M., Zu Horste G.M., Nave K.A., Reis A., Rautenstrauss B. Neurogenetics 10:275-287(2009) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT CMT2B2 VAL-335.
[15]	Erratum Leal A., Huehne K., Bauer F., Sticht H., Berger P., Suter U., Morera B., Del Valle G., Lupski J.R., Ekici A., Pasutto F., Endele S., Barrantes R., Berghoff C., Berghoff M., Neundoerfer B., Heuss D., Dorn T. , Young P., Santolin L., Uhlmann T., Meisterernst M., Sereda M.W., Sereda M., Stassart R.M., Zu Horste G.M., Nave K.A., Reis A., Rautenstrauss B. Neurogenetics 10:375-376(2009)
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF261072 mRNA. Translation: AAM20739.1.
AJ617479 mRNA. Translation: CAE84581.1.
AY533507 mRNA. Translation: AAS45401.1.
AL136746 mRNA. Translation: CAB66680.1. Frameshift.
AK289460 mRNA. Translation: BAF82149.1.
AC006942 Genomic DNA. Translation: AAD15565.1.
CH471177 Genomic DNA. Translation: EAW52546.1.
BC024312 mRNA. Translation: AAH24312.2.
BC065297 mRNA. Translation: AAH65297.1.
AF283769 mRNA. Translation: AAG15589.1. Different initiation.

IPI

IPI00163419.
IPI00418271.
IPI00479592.
IPI00867552.
IPI00867722.

RefSeq

NP_112235.2. NM_030973.3.

UniGene

Hs.656639.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KY6	NMR	-	A	391-553	[»]
2L23	NMR	-	A	391-548	[»]
2L6U	NMR	-	A	391-543	[»]
2XNF	NMR	-	A	394-543	[»]

ProteinModelPortal

Q71SY5.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-31454N.

IntAct

Q71SY5. 7 interactions.

MINT

MINT-5209896.

PTM databases

PhosphoSite

Q71SY5.

Polymorphism databases

DMDM

158706143.

Proteomic databases

PaxDb

Q71SY5.

PRIDE

Q71SY5.

Protocols and materials databases

DNASU

81857.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000312865; ENSP00000326767; ENSG00000104973.

GeneID

81857.

KEGG

hsa:81857.

UCSC

uc002ppw.2. human.

Organism-specific databases

CTD

81857.

GeneCards

GC19P050321.

H-InvDB

HIX0015345.

HGNC

HGNC:28845. MED25.

MIM

605589. phenotype.
610197. gene.

neXtProt

NX_Q71SY5.

Orphanet

101101. Charcot-Marie-Tooth disease type 2B2.

PharmGKB

PA134984839.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG79607.

HOVERGEN

HBG108127.

KO

K15168.

Enzyme and pathway databases

Reactome

REACT_111045. Developmental Biology.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

Q71SY5.

Bgee

Q71SY5.

CleanEx

HS_MED25.

Genevestigator

Q71SY5.

Family and domain databases

InterPro

IPR021394. Mediator_Med25.
IPR021406. Mediator_Med25_NR-box.
IPR021397. Mediator_Med25_SD1.
IPR021419. Mediator_Med25_VWA.
[Graphical view]

Pfam

PF11232. Med25. 1 hit.
PF11244. Med25_NR-box. 1 hit.
PF11235. Med25_SD1. 1 hit.
PF11265. Med25_VWA. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

MED25. human.

EvolutionaryTrace

Q71SY5.

GenomeRNAi

81857.

NextBio

72183.

SOURCE

Search...

Entry information

Entry name

MED25_HUMAN

Accession

Primary (citable) accession number: Q71SY5
Secondary accession number(s): A8K095 , O95783, Q6P143, Q6QMH5, Q707U4, Q8TB55, Q9H0L5, Q9HB34

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 2, 2007
Last modified:	May 1, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.