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Q71SP7

- FAS_BOVIN

UniProt

Q71SP7 - FAS_BOVIN

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Protein

Fatty acid synthase

Gene
FASN
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.

Catalytic activityi

Acetyl-CoA + n malonyl-CoA + 2n NADPH = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP+.
Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein].
Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein].
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H2O.
An acyl-[acyl-carrier protein] + NADP+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH.
Oleoyl-[acyl-carrier-protein] + H2O = [acyl-carrier-protein] + oleate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei161 – 1611For beta-ketoacyl synthase activity By similarity
Active sitei581 – 5811For malonyltransferase activity By similarity
Active sitei878 – 8781For beta-hydroxyacyl dehydratase activity By similarity
Active sitei2310 – 23101For thioesterase activity By similarity
Active sitei2483 – 24831For thioesterase activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1673 – 169018NADP (ER) By similarityAdd
BLAST
Nucleotide bindingi1888 – 190316NADP (KR) By similarityAdd
BLAST

GO - Molecular functioni

  1. [acyl-carrier-protein] S-acetyltransferase activity Source: UniProtKB-EC
  2. [acyl-carrier-protein] S-malonyltransferase activity Source: UniProtKB-EC
  3. 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity Source: UniProtKB-EC
  4. 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity Source: InterPro
  5. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: UniProtKB-EC
  6. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC
  7. enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity Source: UniProtKB-EC
  8. enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity Source: InterPro
  9. myristoyl-[acyl-carrier-protein] hydrolase activity Source: UniProtKB-EC
  10. oleoyl-[acyl-carrier-protein] hydrolase activity Source: UniProtKB-EC
  11. palmitoyl-[acyl-carrier-protein] hydrolase activity Source: UniProtKB-EC
  12. zinc ion binding Source: InterPro

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Lyase, Oxidoreductase, Transferase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD, NADP, Pyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid synthase (EC:2.3.1.85)
Including the following 7 domains:
[Acyl-carrier-protein] S-acetyltransferase (EC:2.3.1.38)
[Acyl-carrier-protein] S-malonyltransferase (EC:2.3.1.39)
3-oxoacyl-[acyl-carrier-protein] synthase (EC:2.3.1.41)
3-oxoacyl-[acyl-carrier-protein] reductase (EC:1.1.1.100)
3-hydroxyacyl-[acyl-carrier-protein] dehydratase (EC:4.2.1.59)
Enoyl-[acyl-carrier-protein] reductase (EC:1.3.1.39)
Oleoyl-[acyl-carrier-protein] hydrolase (EC:3.1.2.14)
Gene namesi
Name:FASN
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Cytoplasm By similarity. Melanosome By similarity

GO - Cellular componenti

  1. melanosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 25132513Fatty acid synthasePRO_0000180274Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei70 – 701N6-acetyllysine By similarity
Modified residuei207 – 2071Phosphoserine By similarity
Modified residuei298 – 2981N6-acetyllysine By similarity
Modified residuei528 – 5281N6-acetyllysine By similarity
Modified residuei673 – 6731N6-acetyllysine By similarity
Modified residuei996 – 9961N6-acetyllysine By similarity
Modified residuei1706 – 17061N6-(pyridoxal phosphate)lysine; alternate By similarity
Modified residuei1706 – 17061N6-acetyllysine; alternate By similarity
Modified residuei1773 – 17731N6-acetyllysine By similarity
Modified residuei1849 – 18491N6-acetyllysine By similarity
Modified residuei1997 – 19971N6-acetyllysine By similarity
Modified residuei2158 – 21581O-(pantetheine 4'-phosphoryl)serine By similarity
Modified residuei2206 – 22061Phosphothreonine By similarity
Modified residuei2238 – 22381Phosphoserine By similarity

Keywords - PTMi

Acetylation, Phosphopantetheine, Phosphoprotein

Proteomic databases

PaxDbiQ71SP7.
PRIDEiQ71SP7.

Interactioni

Subunit structurei

Homodimer which is arranged in a head to tail fashion By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ71SP7.
SMRiQ71SP7. Positions 422-822, 2121-2208, 2223-2509.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2125 – 218157Acyl carrierAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 414414Beta-ketoacyl synthaseAdd
BLAST
Regioni429 – 817389Acyl and malonyl transferasesAdd
BLAST
Regioni1637 – 1865229Enoyl reductaseAdd
BLAST
Regioni1866 – 2119254Beta-ketoacyl reductaseAdd
BLAST
Regioni2209 – 2513305ThioesteraseAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3319.
HOVERGENiHBG005640.
KOiK00665.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
1.10.1470.20. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.
3.40.50.150. 1 hit.
3.40.50.1820. 2 hits.
3.40.50.720. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR013149. ADH_C.
IPR023102. Fatty_acid_synthase_dom_2.
IPR011032. GroES-like.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR016040. NAD(P)-bd_dom.
IPR020842. PKS/FAS_KR.
IPR020843. PKS_ER.
IPR013968. PKS_KR.
IPR006162. PPantetheine_attach_site.
IPR029063. SAM-dependent_MTases-like.
IPR001031. Thioesterase.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 1 hit.
PF00975. Thioesterase. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
SM00822. PKS_KR. 1 hit.
[Graphical view]
SUPFAMiSSF47336. SSF47336. 1 hit.
SSF50129. SSF50129. 1 hit.
SSF52151. SSF52151. 2 hits.
SSF53335. SSF53335. 1 hit.
SSF53474. SSF53474. 1 hit.
SSF53901. SSF53901. 2 hits.
SSF55048. SSF55048. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q71SP7-1 [UniParc]FASTAAdd to Basket

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MEEVVITGMS GKLPESENLE EFWANLIGGV DMVTDDDRRW KAGLYGLPRR     50
SGKLKDLSRF DASFFGVHPK QAHNMDPQLR LLLEVTYEAI VDAGINPASI 100
RGTNTGVWVG VSGSEASEAL SRDPETLVGY SMVGCQRAML ANRLSFFFDF 150
KGPSITLDTA CSSSLLALQR AYQAIQRGEC AMAIVGGVNI RLKPNTSVQF 200
MKLGMLSPEG TCKFFDASGN GYCRAKAVMA ILLTKKSLAR RVYATILNAG 250
TNTDGCKEKG VTFPSGEAQE QLISSLYKPA GLDPETLEYV EAHGTGTKVG 300
DPQELNGIVQ ALCGTRQSPL RIGSTKSNMG HPEPASGLAA LAKVLLSLEH 350
GLWAPNLHFH NPNPKIPALQ DGRLQVVDRP LPVLGGNVGI NSFGFGGSNV 400
HVILQPNSQP LPPPAPHAAL PRLLRASGRT LEGVQGLLEL GLQHSQNLAF 450
VSMLNDIATP SPAAMPFRGY AVLGSQGGSQ KVQQVLAGKR PLWFICSGMG 500
TQWRGMGLSL MRLSRFRDSI LRSDEAVKPL GLQVSQLLLS TDEAIFDDMV 550
ISFVSLTAIQ IALIDLLTSM GLQPDGIIGH SLGEVACGYA DGCISQEEAI 600
LSAYWRGQCI KEANIPPGAM AAVGLTWEEC KQRCPPGIVP ACHNCIDTVT 650
ISGPQASMLE FVQQLKQEGV FAKEVRTGGM AFHSYFMDAI APMLLQQLKK 700
VIREPQPRSP RWLSTSIPET QWQESLARTF SAEYNVNNLV SPVLFQEALW 750
RVPEDAVVLE IAPHALLQAV LKRGLKSSCT IIPLMKKDHR DNLEFFLSNV 800
GQLYLTGIDV NPNGLFPPVE FPAPRGTPLI SPHIKWDHSQ TWDVPTAEDF 850
PSGSSSSSAT IYKIDINPES PDHYLVDHCI DGRIIFPGTG YLCLVWKTLA 900
RALDQNMEHT PVVFEDVTLH QAVILPKTGI VLLKVRLLEA SCTFEVSENG 950
NLIASGKVYQ WEDPNPKLFD NRYGPDPATP VDPTTAIHLS RGDVYKELQL 1000
QGFNYGPYFQ GILEASSEGN TGQLLWKDNW VTFMDTMLQM SILAPSKRSL 1050
RLPTRITAIY IHPATHQQKL YTLQDKTQVA DVVINRCLDT TVAGGIYISR 1100
IHTSVAPRHQ QEQLVPILEK FCFTPHVETG CLAGNLALQE ELQLCVGLAQ 1150
ALQTRVAQQG IKMVVPGLDG AQAPQEAPQQ GLPRLLATAC QLQLNGNLQM 1200
EMGQILAQER ALLCDDPLLS GLLNSPALKA CVTLALENMT SLKMKVVLAG 1250
DGQLYSRIPT LLNTQPLLEL DYTATDRHPQ ALEAAQAKLQ QLDITQGQWD 1300
PSDPAPSNLG GANLVVCNYA LASLGDPATA VGNMVAALKE GGFLLLHTLL 1350
RGHPLGETVT FLTCPEPQQG QRHLLSQDEW ERLFAGASLH LVALKKSFYG 1400
SVLFLCRRLA PLDSPIFLPV EDTSFQWVDS LKNILADSSS RAVWLMAVGC 1450
TTSGVVGLVN CLRKEPDGHR IRCVLVSNLN STSPIPETDP KSLELQKVLQ 1500
SDLVMNVYRD GAWGAFRHFP LEQDKPEEQT EHAFINVLTR GDLSSIRWVC 1550
SPLRHSQPTA PGFQLCTIYY ASLNFKRNHA GHGQAVPRRH PRNWASRNCL 1600
LGMEFSGRDA SGKRVMGLVP AEGLATSTLV PQSFLWDVPS NWTLEEAASV 1650
PVVYSTAYYA LMVRGRMQPG ETVLIHSGSG GVGQAAIAIA LSLGCRVFPL 1700
VGSAEKRAYL QSRFPQLNET SFANSRDTSF EQHVLWHTAG KGADLVLNSL 1750
AEEKLQASVR CLAQHGRFLE IGKFDLSKNH PLGMAIFLKN VTFHGILLDS 1800
LFEENNTMWQ EVSTLLKAGI RKGVVQPLKR TVFPRTQAED AFRYMAQGKH 1850
IGKVVIQVRE EEQEAVLHGT KPTQMVALCK TFCPAHKSYI ITGGLGGFGL 1900
ELAHWLVERG AQKLVLTSRS GIRTGYQARQ VHEWRRQGVQ VLVSTSDVST 1950
LDGTRSLITE AAQLGPVGGI FNLAVVLRDA MLDNQTPEFF QDVNKPKYNG 2000
TLNLDRVTRE ACPELDYFEV FSSVSCGRGN AGQTNYGFAN STMERICEKR 2050
RHDGLPGLAV QWGAIADVGL LMELKGTKDK AIGGTLPQRI TSCMEVLDLF 2100
LNQPHPVLSS FVLAEKATSR GPSGSHQDLV KAVTHILGIR DLATVNLDSS 2150
LSDLGLDSLM GVEVRQMLER EHNLLLSMRE IRQLTIHKLQ EISAQAGTAD 2200
ELTDSTPKFG SPAQSHTQLN LSTLLVNPEG PTLTRLNSVQ SSERPLFLVH 2250
PIEGSTTVFH SLATKLSIPT YGLQCTGAAP LDSIQSLATY YIECIRQVQP 2300
EGNYRIAGYS YGACVAFEMC SQLQAQQNAG PTNNSLFLFD GSHTFVMAYT 2350
QSYRAKLNPG CEAEAEAEAM CFFMQQFTEA EHSRVLEALL PLGDLEARVA 2400
ATVELIVQSH AGLDRHALSF AARSFYHKLR AAEEYTPRAT YHGNVTLLRA 2450
KMGSAYQEGL GADYNLSQVC DGKVSVHIIE GDHRTLLEGS GLESILSIIH 2500
SSLAEPRVSV REG 2513
Length:2,513
Mass (Da):274,554
Last modified:July 5, 2004 - v1
Checksum:iD75B09DB855DFDAB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF285607 Genomic DNA. Translation: AAR19788.1.
AY343889 mRNA. Translation: AAR17600.1.
RefSeqiNP_001012687.1. NM_001012669.1.
UniGeneiBt.30099.

Genome annotation databases

GeneIDi281152.
KEGGibta:281152.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF285607 Genomic DNA. Translation: AAR19788.1 .
AY343889 mRNA. Translation: AAR17600.1 .
RefSeqi NP_001012687.1. NM_001012669.1.
UniGenei Bt.30099.

3D structure databases

ProteinModelPortali Q71SP7.
SMRi Q71SP7. Positions 422-822, 2121-2208, 2223-2509.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q71SP7.
PRIDEi Q71SP7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 281152.
KEGGi bta:281152.

Organism-specific databases

CTDi 2194.

Phylogenomic databases

eggNOGi COG3319.
HOVERGENi HBG005640.
KOi K00665.

Miscellaneous databases

NextBioi 20805215.

Family and domain databases

Gene3Di 1.10.1200.10. 1 hit.
1.10.1470.20. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.
3.40.50.150. 1 hit.
3.40.50.1820. 2 hits.
3.40.50.720. 2 hits.
InterProi IPR029058. AB_hydrolase.
IPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR013149. ADH_C.
IPR023102. Fatty_acid_synthase_dom_2.
IPR011032. GroES-like.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR016040. NAD(P)-bd_dom.
IPR020842. PKS/FAS_KR.
IPR020843. PKS_ER.
IPR013968. PKS_KR.
IPR006162. PPantetheine_attach_site.
IPR029063. SAM-dependent_MTases-like.
IPR001031. Thioesterase.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF00698. Acyl_transf_1. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 1 hit.
PF00975. Thioesterase. 1 hit.
[Graphical view ]
SMARTi SM00829. PKS_ER. 1 hit.
SM00822. PKS_KR. 1 hit.
[Graphical view ]
SUPFAMi SSF47336. SSF47336. 1 hit.
SSF50129. SSF50129. 1 hit.
SSF52151. SSF52151. 2 hits.
SSF53335. SSF53335. 1 hit.
SSF53474. SSF53474. 1 hit.
SSF53901. SSF53901. 2 hits.
SSF55048. SSF55048. 1 hit.
PROSITEi PS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, genomic organization and expression analysis of bovine FASN gene."
    Roy R., Eggen A., Rodellar C.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

Entry informationi

Entry nameiFAS_BOVIN
AccessioniPrimary (citable) accession number: Q71SP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi