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Q71SP7

- FAS_BOVIN

UniProt

Q71SP7 - FAS_BOVIN

Protein

Fatty acid synthase

Gene

FASN

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.

    Catalytic activityi

    Acetyl-CoA + n malonyl-CoA + 2n NADPH = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP+.
    Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein].
    Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein].
    Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].
    (3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.
    A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H2O.
    An acyl-[acyl-carrier protein] + NADP+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH.
    Oleoyl-[acyl-carrier-protein] + H2O = [acyl-carrier-protein] + oleate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei161 – 1611For beta-ketoacyl synthase activityPROSITE-ProRule annotation
    Active sitei581 – 5811For malonyltransferase activityPROSITE-ProRule annotation
    Active sitei878 – 8781For beta-hydroxyacyl dehydratase activityPROSITE-ProRule annotation
    Active sitei2310 – 23101For thioesterase activityPROSITE-ProRule annotation
    Active sitei2483 – 24831For thioesterase activityPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi1673 – 169018NADP (ER)By similarityAdd
    BLAST
    Nucleotide bindingi1888 – 190316NADP (KR)By similarityAdd
    BLAST

    GO - Molecular functioni

    1. [acyl-carrier-protein] S-acetyltransferase activity Source: UniProtKB-EC
    2. [acyl-carrier-protein] S-malonyltransferase activity Source: UniProtKB-EC
    3. 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity Source: UniProtKB-EC
    4. 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity Source: InterPro
    5. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: UniProtKB-EC
    6. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC
    7. enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity Source: UniProtKB-EC
    8. enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity Source: InterPro
    9. myristoyl-[acyl-carrier-protein] hydrolase activity Source: UniProtKB-EC
    10. oleoyl-[acyl-carrier-protein] hydrolase activity Source: UniProtKB-EC
    11. palmitoyl-[acyl-carrier-protein] hydrolase activity Source: UniProtKB-EC
    12. zinc ion binding Source: InterPro

    GO - Biological processi

    1. fatty acid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Lyase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    NAD, NADP, Pyridoxal phosphate

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fatty acid synthase (EC:2.3.1.85)
    Including the following 7 domains:
    [Acyl-carrier-protein] S-acetyltransferase (EC:2.3.1.38)
    [Acyl-carrier-protein] S-malonyltransferase (EC:2.3.1.39)
    3-oxoacyl-[acyl-carrier-protein] synthase (EC:2.3.1.41)
    3-oxoacyl-[acyl-carrier-protein] reductase (EC:1.1.1.100)
    3-hydroxyacyl-[acyl-carrier-protein] dehydratase (EC:4.2.1.59)
    Enoyl-[acyl-carrier-protein] reductase (EC:1.3.1.39)
    Oleoyl-[acyl-carrier-protein] hydrolase (EC:3.1.2.14)
    Gene namesi
    Name:FASN
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Cytoplasm By similarity. Melanosome By similarity

    GO - Cellular componenti

    1. melanosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 25132513Fatty acid synthasePRO_0000180274Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei70 – 701N6-acetyllysineBy similarity
    Modified residuei207 – 2071PhosphoserineBy similarity
    Modified residuei298 – 2981N6-acetyllysineBy similarity
    Modified residuei528 – 5281N6-acetyllysineBy similarity
    Modified residuei673 – 6731N6-acetyllysineBy similarity
    Modified residuei996 – 9961N6-acetyllysineBy similarity
    Modified residuei1706 – 17061N6-(pyridoxal phosphate)lysine; alternateBy similarity
    Modified residuei1706 – 17061N6-acetyllysine; alternateBy similarity
    Modified residuei1773 – 17731N6-acetyllysineBy similarity
    Modified residuei1849 – 18491N6-acetyllysineBy similarity
    Modified residuei1997 – 19971N6-acetyllysineBy similarity
    Modified residuei2158 – 21581O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation
    Modified residuei2206 – 22061PhosphothreonineBy similarity
    Modified residuei2238 – 22381PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphopantetheine, Phosphoprotein

    Proteomic databases

    PaxDbiQ71SP7.
    PRIDEiQ71SP7.

    Interactioni

    Subunit structurei

    Homodimer which is arranged in a head to tail fashion.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ71SP7.
    SMRiQ71SP7. Positions 422-822, 2121-2208, 2223-2509.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2125 – 218157Acyl carrierPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 414414Beta-ketoacyl synthaseAdd
    BLAST
    Regioni429 – 817389Acyl and malonyl transferasesAdd
    BLAST
    Regioni1637 – 1865229Enoyl reductaseAdd
    BLAST
    Regioni1866 – 2119254Beta-ketoacyl reductaseAdd
    BLAST
    Regioni2209 – 2513305ThioesteraseAdd
    BLAST

    Sequence similaritiesi

    Contains 1 acyl carrier domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG3319.
    HOVERGENiHBG005640.
    KOiK00665.

    Family and domain databases

    Gene3Di1.10.1200.10. 1 hit.
    1.10.1470.20. 1 hit.
    3.40.366.10. 2 hits.
    3.40.47.10. 2 hits.
    3.40.50.150. 1 hit.
    3.40.50.1820. 2 hits.
    3.40.50.720. 2 hits.
    InterProiIPR029058. AB_hydrolase.
    IPR001227. Ac_transferase_dom.
    IPR009081. Acyl_carrier_prot-like.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR013149. ADH_C.
    IPR023102. Fatty_acid_synthase_dom_2.
    IPR011032. GroES-like.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016036. Malonyl_transacylase_ACP-bd.
    IPR016040. NAD(P)-bd_dom.
    IPR020842. PKS/FAS_KR.
    IPR020843. PKS_ER.
    IPR013968. PKS_KR.
    IPR006162. PPantetheine_attach_site.
    IPR029063. SAM-dependent_MTases-like.
    IPR001031. Thioesterase.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF00698. Acyl_transf_1. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    PF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    PF08659. KR. 1 hit.
    PF00550. PP-binding. 1 hit.
    PF00975. Thioesterase. 1 hit.
    [Graphical view]
    SMARTiSM00829. PKS_ER. 1 hit.
    SM00822. PKS_KR. 1 hit.
    [Graphical view]
    SUPFAMiSSF47336. SSF47336. 1 hit.
    SSF50129. SSF50129. 1 hit.
    SSF52151. SSF52151. 2 hits.
    SSF53335. SSF53335. 1 hit.
    SSF53474. SSF53474. 1 hit.
    SSF53901. SSF53901. 2 hits.
    SSF55048. SSF55048. 1 hit.
    PROSITEiPS50075. ACP_DOMAIN. 1 hit.
    PS00606. B_KETOACYL_SYNTHASE. 1 hit.
    PS00012. PHOSPHOPANTETHEINE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q71SP7-1 [UniParc]FASTAAdd to Basket

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    MEEVVITGMS GKLPESENLE EFWANLIGGV DMVTDDDRRW KAGLYGLPRR     50
    SGKLKDLSRF DASFFGVHPK QAHNMDPQLR LLLEVTYEAI VDAGINPASI 100
    RGTNTGVWVG VSGSEASEAL SRDPETLVGY SMVGCQRAML ANRLSFFFDF 150
    KGPSITLDTA CSSSLLALQR AYQAIQRGEC AMAIVGGVNI RLKPNTSVQF 200
    MKLGMLSPEG TCKFFDASGN GYCRAKAVMA ILLTKKSLAR RVYATILNAG 250
    TNTDGCKEKG VTFPSGEAQE QLISSLYKPA GLDPETLEYV EAHGTGTKVG 300
    DPQELNGIVQ ALCGTRQSPL RIGSTKSNMG HPEPASGLAA LAKVLLSLEH 350
    GLWAPNLHFH NPNPKIPALQ DGRLQVVDRP LPVLGGNVGI NSFGFGGSNV 400
    HVILQPNSQP LPPPAPHAAL PRLLRASGRT LEGVQGLLEL GLQHSQNLAF 450
    VSMLNDIATP SPAAMPFRGY AVLGSQGGSQ KVQQVLAGKR PLWFICSGMG 500
    TQWRGMGLSL MRLSRFRDSI LRSDEAVKPL GLQVSQLLLS TDEAIFDDMV 550
    ISFVSLTAIQ IALIDLLTSM GLQPDGIIGH SLGEVACGYA DGCISQEEAI 600
    LSAYWRGQCI KEANIPPGAM AAVGLTWEEC KQRCPPGIVP ACHNCIDTVT 650
    ISGPQASMLE FVQQLKQEGV FAKEVRTGGM AFHSYFMDAI APMLLQQLKK 700
    VIREPQPRSP RWLSTSIPET QWQESLARTF SAEYNVNNLV SPVLFQEALW 750
    RVPEDAVVLE IAPHALLQAV LKRGLKSSCT IIPLMKKDHR DNLEFFLSNV 800
    GQLYLTGIDV NPNGLFPPVE FPAPRGTPLI SPHIKWDHSQ TWDVPTAEDF 850
    PSGSSSSSAT IYKIDINPES PDHYLVDHCI DGRIIFPGTG YLCLVWKTLA 900
    RALDQNMEHT PVVFEDVTLH QAVILPKTGI VLLKVRLLEA SCTFEVSENG 950
    NLIASGKVYQ WEDPNPKLFD NRYGPDPATP VDPTTAIHLS RGDVYKELQL 1000
    QGFNYGPYFQ GILEASSEGN TGQLLWKDNW VTFMDTMLQM SILAPSKRSL 1050
    RLPTRITAIY IHPATHQQKL YTLQDKTQVA DVVINRCLDT TVAGGIYISR 1100
    IHTSVAPRHQ QEQLVPILEK FCFTPHVETG CLAGNLALQE ELQLCVGLAQ 1150
    ALQTRVAQQG IKMVVPGLDG AQAPQEAPQQ GLPRLLATAC QLQLNGNLQM 1200
    EMGQILAQER ALLCDDPLLS GLLNSPALKA CVTLALENMT SLKMKVVLAG 1250
    DGQLYSRIPT LLNTQPLLEL DYTATDRHPQ ALEAAQAKLQ QLDITQGQWD 1300
    PSDPAPSNLG GANLVVCNYA LASLGDPATA VGNMVAALKE GGFLLLHTLL 1350
    RGHPLGETVT FLTCPEPQQG QRHLLSQDEW ERLFAGASLH LVALKKSFYG 1400
    SVLFLCRRLA PLDSPIFLPV EDTSFQWVDS LKNILADSSS RAVWLMAVGC 1450
    TTSGVVGLVN CLRKEPDGHR IRCVLVSNLN STSPIPETDP KSLELQKVLQ 1500
    SDLVMNVYRD GAWGAFRHFP LEQDKPEEQT EHAFINVLTR GDLSSIRWVC 1550
    SPLRHSQPTA PGFQLCTIYY ASLNFKRNHA GHGQAVPRRH PRNWASRNCL 1600
    LGMEFSGRDA SGKRVMGLVP AEGLATSTLV PQSFLWDVPS NWTLEEAASV 1650
    PVVYSTAYYA LMVRGRMQPG ETVLIHSGSG GVGQAAIAIA LSLGCRVFPL 1700
    VGSAEKRAYL QSRFPQLNET SFANSRDTSF EQHVLWHTAG KGADLVLNSL 1750
    AEEKLQASVR CLAQHGRFLE IGKFDLSKNH PLGMAIFLKN VTFHGILLDS 1800
    LFEENNTMWQ EVSTLLKAGI RKGVVQPLKR TVFPRTQAED AFRYMAQGKH 1850
    IGKVVIQVRE EEQEAVLHGT KPTQMVALCK TFCPAHKSYI ITGGLGGFGL 1900
    ELAHWLVERG AQKLVLTSRS GIRTGYQARQ VHEWRRQGVQ VLVSTSDVST 1950
    LDGTRSLITE AAQLGPVGGI FNLAVVLRDA MLDNQTPEFF QDVNKPKYNG 2000
    TLNLDRVTRE ACPELDYFEV FSSVSCGRGN AGQTNYGFAN STMERICEKR 2050
    RHDGLPGLAV QWGAIADVGL LMELKGTKDK AIGGTLPQRI TSCMEVLDLF 2100
    LNQPHPVLSS FVLAEKATSR GPSGSHQDLV KAVTHILGIR DLATVNLDSS 2150
    LSDLGLDSLM GVEVRQMLER EHNLLLSMRE IRQLTIHKLQ EISAQAGTAD 2200
    ELTDSTPKFG SPAQSHTQLN LSTLLVNPEG PTLTRLNSVQ SSERPLFLVH 2250
    PIEGSTTVFH SLATKLSIPT YGLQCTGAAP LDSIQSLATY YIECIRQVQP 2300
    EGNYRIAGYS YGACVAFEMC SQLQAQQNAG PTNNSLFLFD GSHTFVMAYT 2350
    QSYRAKLNPG CEAEAEAEAM CFFMQQFTEA EHSRVLEALL PLGDLEARVA 2400
    ATVELIVQSH AGLDRHALSF AARSFYHKLR AAEEYTPRAT YHGNVTLLRA 2450
    KMGSAYQEGL GADYNLSQVC DGKVSVHIIE GDHRTLLEGS GLESILSIIH 2500
    SSLAEPRVSV REG 2513
    Length:2,513
    Mass (Da):274,554
    Last modified:July 5, 2004 - v1
    Checksum:iD75B09DB855DFDAB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF285607 Genomic DNA. Translation: AAR19788.1.
    AY343889 mRNA. Translation: AAR17600.1.
    RefSeqiNP_001012687.1. NM_001012669.1.
    UniGeneiBt.30099.

    Genome annotation databases

    GeneIDi281152.
    KEGGibta:281152.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF285607 Genomic DNA. Translation: AAR19788.1 .
    AY343889 mRNA. Translation: AAR17600.1 .
    RefSeqi NP_001012687.1. NM_001012669.1.
    UniGenei Bt.30099.

    3D structure databases

    ProteinModelPortali Q71SP7.
    SMRi Q71SP7. Positions 422-822, 2121-2208, 2223-2509.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q71SP7.
    PRIDEi Q71SP7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 281152.
    KEGGi bta:281152.

    Organism-specific databases

    CTDi 2194.

    Phylogenomic databases

    eggNOGi COG3319.
    HOVERGENi HBG005640.
    KOi K00665.

    Miscellaneous databases

    NextBioi 20805215.

    Family and domain databases

    Gene3Di 1.10.1200.10. 1 hit.
    1.10.1470.20. 1 hit.
    3.40.366.10. 2 hits.
    3.40.47.10. 2 hits.
    3.40.50.150. 1 hit.
    3.40.50.1820. 2 hits.
    3.40.50.720. 2 hits.
    InterProi IPR029058. AB_hydrolase.
    IPR001227. Ac_transferase_dom.
    IPR009081. Acyl_carrier_prot-like.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR013149. ADH_C.
    IPR023102. Fatty_acid_synthase_dom_2.
    IPR011032. GroES-like.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016036. Malonyl_transacylase_ACP-bd.
    IPR016040. NAD(P)-bd_dom.
    IPR020842. PKS/FAS_KR.
    IPR020843. PKS_ER.
    IPR013968. PKS_KR.
    IPR006162. PPantetheine_attach_site.
    IPR029063. SAM-dependent_MTases-like.
    IPR001031. Thioesterase.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF00698. Acyl_transf_1. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    PF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    PF08659. KR. 1 hit.
    PF00550. PP-binding. 1 hit.
    PF00975. Thioesterase. 1 hit.
    [Graphical view ]
    SMARTi SM00829. PKS_ER. 1 hit.
    SM00822. PKS_KR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47336. SSF47336. 1 hit.
    SSF50129. SSF50129. 1 hit.
    SSF52151. SSF52151. 2 hits.
    SSF53335. SSF53335. 1 hit.
    SSF53474. SSF53474. 1 hit.
    SSF53901. SSF53901. 2 hits.
    SSF55048. SSF55048. 1 hit.
    PROSITEi PS50075. ACP_DOMAIN. 1 hit.
    PS00606. B_KETOACYL_SYNTHASE. 1 hit.
    PS00012. PHOSPHOPANTETHEINE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, genomic organization and expression analysis of bovine FASN gene."
      Roy R., Eggen A., Rodellar C.
      Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

    Entry informationi

    Entry nameiFAS_BOVIN
    AccessioniPrimary (citable) accession number: Q71SP7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3