Q71RC2 (LARP4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
La-related protein 4

Alternative name(s):
La ribonucleoprotein domain family member 4

Gene names

Name:	LARP4
ORF Names:	PP13296

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the regulation of cell morphology and cytoskeletal organization. Ref.8
Sequence similarities	Contains 1 HTH La-type RNA-binding domain. Contains 1 RRM (RNA recognition motif) domain.
Sequence caution	The sequence BAC86052.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	RNA-binding
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cytoskeleton organization Inferred from mutant phenotype Ref.8. Source: UniProtKB regulation of cell morphogenesis Inferred from mutant phenotype Ref.8. Source: UniProtKB
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q71RC2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q71RC2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     7-7: Missing.
     97-194: Missing.
     593-612: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q71RC2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     7-7: Missing.

Note: May be due to competing acceptor splice site. No experimental confirmation available.

Isoform 4 (identifier: Q71RC2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     55-55: E → EVSVFNT

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 724

724

La-related protein 4

PRO_0000207611

Regions

Domain

113 – 202

90

HTH La-type RNA-binding

Domain

203 – 281

79

RRM

Amino acid modifications

Modified residue

1

1

N-acetylmethionine Ref.4 Ref.6

Modified residue

392

1

Phosphoserine Ref.5 Ref.7

Modified residue

583

1

Phosphoserine Ref.5 Ref.6

Modified residue

586

1

Phosphoserine Ref.5

Modified residue

595

1

Phosphothreonine Ref.6

Modified residue

597

1

Phosphoserine Ref.6

Modified residue

643

1

Phosphoserine Ref.6

Modified residue

647

1

Phosphoserine Ref.6

Modified residue

649

1

Phosphothreonine Ref.6

Modified residue

722

1

Phosphoserine Ref.5

Natural variations

Alternative sequence

7

1

Missing in isoform 2 and isoform 3.

VSP_033811

Alternative sequence

55

1

E → EVSVFNT in isoform 4.

VSP_033812

Alternative sequence

97 – 194

98

Missing in isoform 2.

VSP_014611

Alternative sequence

593 – 612

20

Missing in isoform 2.

VSP_014612

Natural variant

351

1

F → L.
Corresponds to variant rs17124706 [ dbSNP | Ensembl ].

VAR_055936

Natural variant

502

1

N → T. Ref.2
Corresponds to variant rs17124715 [ dbSNP | Ensembl ].

VAR_055937

Experimental info

Sequence conflict

639

1

R → Q in BAF84435. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

724

Helix Strand Turn

Details...

Helix

115 – 129

15

Helix

132 – 137

6

Helix

139 – 144

6

Helix

153 – 158

6

Helix

160 – 165

6

Helix

169 – 178

10

Beta strand

179 – 184

6

Beta strand

186 – 193

8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 20, 2008. Version 3. Checksum: 93B8C2D8CB3CDAEF	FASTA	724	80,596
	10 20 30 40 50 60 MLLFVEQVAS KGTGLNPNAK VWQEIAPGNT DATPVTHGTE SSWHEIAATS GAHPEGNAEL 70 80 90 100 110 120 SEDICKEYEV MYSSSCETTR NTTGIEESTD GMILGPEDLS YQIYDVSGES NSAVSTEDLK 130 140 150 160 170 180 ECLKKQLEFC FSRENLSKDL YLISQMDSDQ FIPIWTVANM EEIKKLTTDP DLILEVLRSS 190 200 210 220 230 240 PMVQVDEKGE KVRPSHKRCI VILREIPETT PIEEVKGLFK SENCPKVISC EFAHNSNWYI 250 260 270 280 290 300 TFQSDTDAQQ AFKYLREEVK TFQGKPIMAR IKAINTFFAK NGYRLMDSSI YSHPIQTQAQ 310 320 330 340 350 360 YASPVFMQPV YNPHQQYSVY SIVPQSWSPN PTPYFETPLA PFPNGSFVNG FNSPGSYKTN 370 380 390 400 410 420 AAAMNMGRPF QKNRVKPQFR SSGGSEHSTE GSVSLGDGQL NRYSSRNFPA ERHNPTVTGH 430 440 450 460 470 480 QEQTYLQKET STLQVEQNGD YGRGRRTLFR GRRRREDDRI SRPHPSTAES KAPTPKFDLL 490 500 510 520 530 540 ASNFPPLPGS SSRMPGELVL ENRMSDVVKG VYKEKDNEEL TISCPVPADE QTECTSAQQL 550 560 570 580 590 600 NMSTSSPCAA ELTALSTTQQ EKDLIEDSSV QKDGLNQTTI PVSPPSTTKP SRASTASPCN 610 620 630 640 650 660 NNINAATAVA LQEPRKLSYA EVCQKPPKEP SSVLVQPLRE LRSNVVSPTK NEDNGAPENS 670 680 690 700 710 720 VEKPHEKPEA RASKDYSGFR GNIIPRGAAG KIREQRRQFS HRAIPQGVTR RNGKEQYVPP RSPK
	Isoform 2 [UniParc]. Checksum: 8BBA0DFC9487ACD1	FASTA	605	67,280
	10 20 30 40 50 60 MLLFVEVASK GTGLNPNAKV WQEIAPGNTD ATPVTHGTES SWHEIAATSG AHPEGNAELS 70 80 90 100 110 120 EDICKEYEVM YSSSCETTRN TTGIEESTDG MILGPSHKRC IVILREIPET TPIEEVKGLF 130 140 150 160 170 180 KSENCPKVIS CEFAHNSNWY ITFQSDTDAQ QAFKYLREEV KTFQGKPIMA RIKAINTFFA 190 200 210 220 230 240 KNGYRLMDSS IYSHPIQTQA QYASPVFMQP VYNPHQQYSV YSIVPQSWSP NPTPYFETPL 250 260 270 280 290 300 APFPNGSFVN GFNSPGSYKT NAAAMNMGRP FQKNRVKPQF RSSGGSEHST EGSVSLGDGQ 310 320 330 340 350 360 LNRYSSRNFP AERHNPTVTG HQEQTYLQKE TSTLQVEQNG DYGRGRRTLF RGRRRREDDR 370 380 390 400 410 420 ISRPHPSTAE SKAPTPKFDL LASNFPPLPG SSSRMPGELV LENRMSDVVK GVYKEKDNEE 430 440 450 460 470 480 LTISCPVPAD EQTECTSAQQ LNMSTSSPCA AELTALSTTQ QEKDLIEDSS VQKDGLNQTT 490 500 510 520 530 540 IPVSPPSTTK PSREPRKLSY AEVCQKPPKE PSSVLVQPLR ELRSNVVSPT KNEDNGAPEN 550 560 570 580 590 600 SVEKPHEKPE ARASKDYSGF RGNIIPRGAA GKIREQRRQF SHRAIPQGVT RRNGKEQYVP PRSPK
	Isoform 3 [UniParc]. Checksum: 3FD2F26A384908DB	FASTA	723	80,468
	10 20 30 40 50 60 MLLFVEVASK GTGLNPNAKV WQEIAPGNTD ATPVTHGTES SWHEIAATSG AHPEGNAELS 70 80 90 100 110 120 EDICKEYEVM YSSSCETTRN TTGIEESTDG MILGPEDLSY QIYDVSGESN SAVSTEDLKE 130 140 150 160 170 180 CLKKQLEFCF SRENLSKDLY LISQMDSDQF IPIWTVANME EIKKLTTDPD LILEVLRSSP 190 200 210 220 230 240 MVQVDEKGEK VRPSHKRCIV ILREIPETTP IEEVKGLFKS ENCPKVISCE FAHNSNWYIT 250 260 270 280 290 300 FQSDTDAQQA FKYLREEVKT FQGKPIMARI KAINTFFAKN GYRLMDSSIY SHPIQTQAQY 310 320 330 340 350 360 ASPVFMQPVY NPHQQYSVYS IVPQSWSPNP TPYFETPLAP FPNGSFVNGF NSPGSYKTNA 370 380 390 400 410 420 AAMNMGRPFQ KNRVKPQFRS SGGSEHSTEG SVSLGDGQLN RYSSRNFPAE RHNPTVTGHQ 430 440 450 460 470 480 EQTYLQKETS TLQVEQNGDY GRGRRTLFRG RRRREDDRIS RPHPSTAESK APTPKFDLLA 490 500 510 520 530 540 SNFPPLPGSS SRMPGELVLE NRMSDVVKGV YKEKDNEELT ISCPVPADEQ TECTSAQQLN 550 560 570 580 590 600 MSTSSPCAAE LTALSTTQQE KDLIEDSSVQ KDGLNQTTIP VSPPSTTKPS RASTASPCNN 610 620 630 640 650 660 NINAATAVAL QEPRKLSYAE VCQKPPKEPS SVLVQPLREL RSNVVSPTKN EDNGAPENSV 670 680 690 700 710 720 EKPHEKPEAR ASKDYSGFRG NIIPRGAAGK IREQRRQFSH RAIPQGVTRR NGKEQYVPPR SPK
	Isoform 4 [UniParc]. Checksum: 0E1FED65589E925A	FASTA	730	81,244
	10 20 30 40 50 60 MLLFVEQVAS KGTGLNPNAK VWQEIAPGNT DATPVTHGTE SSWHEIAATS GAHPEVSVFN 70 80 90 100 110 120 TGNAELSEDI CKEYEVMYSS SCETTRNTTG IEESTDGMIL GPEDLSYQIY DVSGESNSAV 130 140 150 160 170 180 STEDLKECLK KQLEFCFSRE NLSKDLYLIS QMDSDQFIPI WTVANMEEIK KLTTDPDLIL 190 200 210 220 230 240 EVLRSSPMVQ VDEKGEKVRP SHKRCIVILR EIPETTPIEE VKGLFKSENC PKVISCEFAH 250 260 270 280 290 300 NSNWYITFQS DTDAQQAFKY LREEVKTFQG KPIMARIKAI NTFFAKNGYR LMDSSIYSHP 310 320 330 340 350 360 IQTQAQYASP VFMQPVYNPH QQYSVYSIVP QSWSPNPTPY FETPLAPFPN GSFVNGFNSP 370 380 390 400 410 420 GSYKTNAAAM NMGRPFQKNR VKPQFRSSGG SEHSTEGSVS LGDGQLNRYS SRNFPAERHN 430 440 450 460 470 480 PTVTGHQEQT YLQKETSTLQ VEQNGDYGRG RRTLFRGRRR REDDRISRPH PSTAESKAPT 490 500 510 520 530 540 PKFDLLASNF PPLPGSSSRM PGELVLENRM SDVVKGVYKE KDNEELTISC PVPADEQTEC 550 560 570 580 590 600 TSAQQLNMST SSPCAAELTA LSTTQQEKDL IEDSSVQKDG LNQTTIPVSP PSTTKPSRAS 610 620 630 640 650 660 TASPCNNNIN AATAVALQEP RKLSYAEVCQ KPPKEPSSVL VQPLRELRSN VVSPTKNEDN 670 680 690 700 710 720 GAPENSVEKP HEKPEARASK DYSGFRGNII PRGAAGKIRE QRRQFSHRAI PQGVTRRNGK 730 EQYVPPRSPK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 10-724 (ISOFORM 4). Tissue: Placenta and Tongue.
[2]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed: 15498874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANT THR-502.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Cervix.
[4]	Bienvenut W.V., Heiserich L., Boulahbel H., Gottlieb E. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-11; 166-178; 205-216 AND 494-503, ACETYLATION AT MET-1, MASS SPECTROMETRY. Tissue: Colon carcinoma.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-392; SER-583; SER-586 AND SER-722, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-583; THR-595; SER-597; SER-643; SER-647 AND THR-649, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-392, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[8]	"Identification and characterization of a set of conserved and new regulators of cytoskeletal organisation, cell morphology and migration." Bai S.W., Herrera-Abreu M.T., Rohn J.L., Racine V., Tajadura V., Suryavanshi N., Bechtel S., Wiemann S., Baum B., Ridley A.J. BMC Biol. 9:54-54(2011) [PubMed: 21834987] [Abstract] Cited for: FUNCTION.
[9]	"Solution structure of the La domain of c-mpl binding protein." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 112-199.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK055521 mRNA. Translation: BAB70940.1.
AK125113 mRNA. Translation: BAC86052.1. Different initiation.
AK291746 mRNA. Translation: BAF84435.1.
AF370416 mRNA. Translation: AAQ15252.1.
CR936626 mRNA. Translation: CAI56769.1.

IPI

IPI00043638.
IPI00395347.
IPI00797384.
IPI00982060.

RefSeq

NP_001164279.1. NM_001170808.1.
NP_443111.4. NM_052879.4.
NP_954658.2. NM_199188.2.

UniGene

Hs.26613.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CQK	NMR	-	A	113-200	[»]
3PKN	X-ray	1.80	B	13-26	[»]

ProteinModelPortal

Q71RC2.

SMR

Q71RC2. Positions 113-200.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q71RC2. 1 interaction.

STRING

Q71RC2.

PTM databases

PhosphoSite

Q71RC2.

Polymorphism databases

DMDM

189047131.

Proteomic databases

PRIDE

Q71RC2.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000398473; ENSP00000381490; ENSG00000161813.

GeneID

113251.

KEGG

hsa:113251.

UCSC

uc001rwo.1. human.
uc001rwp.1. human.
uc001rws.1. human.
uc009zlr.1. human.

Organism-specific databases

CTD

113251.

GeneCards

GC12P050794.

H-InvDB

HIX0010628.

HGNC

HGNC:24320. LARP4.

HPA

HPA039306.
HPA039673.

neXtProt

NX_Q71RC2.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG17168.

HOVERGEN

HBG055137.

OMA

RVSCPVP.

Gene expression databases

ArrayExpress

Q71RC2.

Bgee

Q71RC2.

CleanEx

HS_LARP4.

Genevestigator

Q71RC2.

GermOnline

ENSG00000161813. Homo sapiens.

Family and domain databases

InterPro

IPR006630. Lupus_La_RNA-bd.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]

Gene3D

G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.

Pfam

PF05383. La. 1 hit.
[Graphical view]

SMART

SM00715. LA. 1 hit.
SM00360. RRM. 1 hit.
[Graphical view]

PROSITE

PS50961. HTH_LA. 1 hit.
PS50102. RRM. False negative.
[Graphical view]

ProtoNet

Search...

Other

NextBio

78808.

PMAP-CutDB

Q71RC2.

Entry information

Entry name

LARP4_HUMAN

Accession

Primary (citable) accession number: Q71RC2
Secondary accession number(s): A8K6T1 , Q5CZ97, Q6ZV14, Q96NF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.