Reviewed, UniProtKB/Swiss-Prot Q71QF9 (PRIC1_RAT)

Names and origin

Protein names

Recommended name:
    Prickle-like protein 1
Alternative name(s):
    REST/NRSF-interacting LIM domain protein 1

Gene names

Name:	Prickle1
Synonyms:	Rilp

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	831 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Necessary for nuclear localization of REST. May serve as nuclear receptor By similarity.
Subunit structure	Interacts with REST By similarity.
Subcellular location	Nucleus membrane By similarity. Cytoplasm › cytosol By similarity. Note: A smaller amount is detected in the cytosol By similarity.
Sequence similarities	Belongs to the prickle / espinas / testin family. Contains 3 LIM zinc-binding domains. Contains 1 PET domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane Nucleus
Domain	LIM domain Repeat
Ligand	Metal-binding Zinc
PTM	Lipoprotein Phosphoprotein Prenylation
Gene Ontology (GO)
Biological process	protein import into nucleus Ref.2 Inferred from direct assay. Source: RGD
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transcription factor binding Ref.2 Inferred from physical interaction. Source: RGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 831	831	Prickle-like protein 1		PRO_0000075890
Regions
	Domain	14 – 122	109	PET
	Domain	124 – 189	66	LIM zinc-binding 1
	Domain	189 – 249	61	LIM zinc-binding 2
	Domain	249 – 313	65	LIM zinc-binding 3
	Compositional bias	757 – 760	4	Poly-Asp
	Compositional bias	816 – 821	6	Poly-Lys
Amino acid modifications
	Modified residue	681	1	Phosphoserine By similarity
	Lipidation	828	1	S-farnesyl cysteine By similarity
Experimental info
	Sequence conflict	115	1	K → I in AW434734. Ref.1
	Sequence conflict	121	1	M → T Ref.2
	Sequence conflict	125	1	V → L in AW434734. Ref.1
	Sequence conflict	133	1	M → I Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q71QF9-1 [UniParc]. Last modified February 1, 2005. Version 2. Checksum: 25B999F11055FBD1	FASTA	831	94,170
	10 20 30 40 50 60 MPLEMEPKMS KLAFGCQRSS TSDDDSGCAL EEYAWVPPGL RPEQIQLYFA CLPEEKVPYV 70 80 90 100 110 120 NSPGEKHRIK QLLYQLPPHD NEVRYCQSLS EEEKKELQVF SAQRKKEALG RGTIKLLSRA 130 140 150 160 170 180 MMHAVCEQCG LQMNGGEVAV FASRAGPGVC WRPSCFVCFT CNELLVDLIY FYQDGKIHCG 190 200 210 220 230 240 RHHAELLKPR CSACDEIIFA DECTEAEGRH WHMKHFCCLE CETVLGGQRY IMKDGRPFCC 250 260 270 280 290 300 GCFESLYAEY CETCGEHIGV DHAQMTYDGQ HWHATEACFS CAQCKASLLG CPFLPKQGQI 310 320 330 340 350 360 YCSKTCSLGE DIHASDSSDS AFQSARSRDS RRSVRMGRSS RSADQCRQSL LLSPALNYKF 370 380 390 400 410 420 PGLSGSADDT LSRKLDDVSL SGQGAGFAHE EFWKARVDQE ASEDPEEWAE HEDYMTQLLL 430 440 450 460 470 480 KFGDKNLFQQ PPSEVDMRAS EHWIPDNMVT NKPEAKQNHQ SLASKKYQSD MYWAQSQDGL 490 500 510 520 530 540 GDSAYGSHPG PASSRRLQEL DLDHGAAGYN HDQTQWYEDS LECLSDLKPE QSVRDSMDSL 550 560 570 580 590 600 ALSNITGASV DGESKPRPSL YSLQNFEEIE AEDCEKMSNM GTLNSSMLHR SAESLKSLNS 610 620 630 640 650 660 ELCPEKIIPE EKPVHLPVLR RSKSQSRPQQ VKFSDDVIDN GSYDIEIRQP PMSERTRRRV 670 680 690 700 710 720 YHFEERGSRP HHHRHRRSRK SRSDNALNLV TERKYSAKDR LRLYTPDNYE KFIQSKGARE 730 740 750 760 770 780 LQAYMQNANL YGQYAHTTSD YALQNPGMTR FLGLYGDDDD SWCSSSTSSS DSEEEGYFLG 790 800 810 820 830 QPIPQPRPQR FTYYTDDLSS PASALPTPQF NQRTTKSKKK KGHRGKNCII S

References

[1]	Program for rat gene discovery and mapping Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-135.
[2]	"REST/NRSF-interacting LIM domain protein, a putative nuclear translocation receptor." Shimojo M., Hersh L.B. Mol. Cell. Biol. 23:9025-9031(2003) [PubMed: 14645515] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 86-831. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AW434734 mRNA. No translation available. AF399843 mRNA. Translation: AAQ03034.1. Different initiation.
IPI	IPI00361687.
UniGene	Rn.141040
3D structure databases
SMR	Q71QF9. Positions 124-262.
ModBase	Search...
Protein-protein interaction databases
STRING	Q71QF9.
PTM databases
PhosphoSite	Q71QF9.
Genome annotation databases
Ensembl	ENSRNOT00000036750; ENSRNOP00000031476; ENSRNOG00000022772; Rattus norvegicus. [Genome view]
Organism-specific databases
RGD	735090. Prickle1.
Phylogenomic databases
eggNOG	roNOG13089.
HOVERGEN	Q71QF9.
InParanoid	Q71QF9.
Gene expression databases
ArrayExpress	Q71QF9.
Genevestigator	Q71QF9.
GermOnline	ENSRNOG00000022772. Rattus norvegicus.
Family and domain databases
InterPro	IPR010442. PET_domain. IPR001781. Znf_LIM. [Graphical view]
Gene3D	G3DSA:2.10.110.10. Znf_LIM. 2 hits.
Pfam	PF00412. LIM. 3 hits. PF06297. PET. 1 hit. [Graphical view]
SMART	SM00132. LIM. 3 hits. [Graphical view]
PROSITE	PS00478. LIM_DOMAIN_1. 1 hit. PS50023. LIM_DOMAIN_2. 3 hits. PS51303. PET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRIC1_RAT

Accession

Primary (citable) accession number: Q71QF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	February 1, 2005
Last modified:	January 19, 2010
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)