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Protein

Guanidinoacetate N-methyltransferase

Gene

gamt

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Converts guanidinoacetate to creatine, using S-adenosylmethionine as the methyl donor. Important in nervous system development.By similarity

Catalytic activityi

S-adenosyl-L-methionine + guanidinoacetate = S-adenosyl-L-homocysteine + creatine.PROSITE-ProRule annotation

Pathwayi: creatine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes creatine from L-arginine and glycine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glycine amidinotransferase, mitochondrial (gatm)
  2. Guanidinoacetate N-methyltransferase (gamt)
This subpathway is part of the pathway creatine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes creatine from L-arginine and glycine, the pathway creatine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei18 – 181S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei40 – 401SubstratePROSITE-ProRule annotation
Binding sitei44 – 441SubstratePROSITE-ProRule annotation
Binding sitei48 – 481S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei133 – 1331S-adenosyl-L-methionine and substratePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-DRE-71288. Creatine metabolism.
UniPathwayiUPA00104; UER00580.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanidinoacetate N-methyltransferase (EC:2.1.1.2)
Gene namesi
Name:gamt
ORF Names:zgc:123136
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 11

Organism-specific databases

ZFINiZDB-GENE-051030-97. gamt.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 234234Guanidinoacetate N-methyltransferasePRO_0000228965Add
BLAST

Proteomic databases

PaxDbiQ71N41.

Expressioni

Gene expression databases

BgeeiQ71N41.
ExpressionAtlasiQ71N41. differential.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000095135.

Structurei

3D structure databases

ProteinModelPortaliQ71N41.
SMRiQ71N41. Positions 6-233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 234234RMT2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 726S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
Regioni88 – 903S-adenosyl-L-methioninePROSITE-ProRule annotation
Regioni115 – 1162S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
Regioni169 – 1702Substrate bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family.PROSITE-ProRule annotation
Contains 1 RMT2 (arginine N-methyltransferase 2-like) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410ISNM. Eukaryota.
ENOG410XPX4. LUCA.
GeneTreeiENSGT00390000018061.
HOGENOMiHOG000010290.
HOVERGENiHBG005801.
InParanoidiQ71N41.
KOiK00542.
OMAiRYYAFPQ.
OrthoDBiEOG75QR4S.
PhylomeDBiQ71N41.
TreeFamiTF328555.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR016550. GuanidinoAc_N-MeTrfase.
IPR026480. RMT2_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PIRSFiPIRSF009285. GAMT. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51559. SAM_RMT2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q71N41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAAQPIFSK GENCKQVWHD ANADYNAADT HLEIMGKPVM ERWETPYMHS
60 70 80 90 100
LATVAASKGG RVLEIGFGMA IAATKVESFP IEEHWIIECN DGVFQRLQEW
110 120 130 140 150
AKSQPHKVVP LKGLWEEVAP TLPDNHFDGI LYDTYPLSEE TWHTHQFNFI
160 170 180 190 200
KAHAHRMLKP GGVLTYCNLT SWGELLKNKY DNIDKMFQET QVPHLLEAGF
210 220 230
KKEKISTTLM DISPPSECKY YSFNKMITPT IIKE
Length:234
Mass (Da):26,743
Last modified:March 21, 2006 - v2
Checksum:iC998B222A2FA1E1F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711I → T in AAQ13341 (Ref. 1) Curated
Sequence conflicti225 – 2251K → R in AAQ13341 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF425745 mRNA. Translation: AAQ13341.1.
BC107607 mRNA. Translation: AAI07608.1.
RefSeqiNP_001099065.1. NM_001105595.1.
UniGeneiDr.28451.

Genome annotation databases

EnsembliENSDART00000104360; ENSDARP00000095135; ENSDARG00000070844.
GeneIDi796865.
KEGGidre:796865.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF425745 mRNA. Translation: AAQ13341.1.
BC107607 mRNA. Translation: AAI07608.1.
RefSeqiNP_001099065.1. NM_001105595.1.
UniGeneiDr.28451.

3D structure databases

ProteinModelPortaliQ71N41.
SMRiQ71N41. Positions 6-233.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000095135.

Proteomic databases

PaxDbiQ71N41.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000104360; ENSDARP00000095135; ENSDARG00000070844.
GeneIDi796865.
KEGGidre:796865.

Organism-specific databases

CTDi2593.
ZFINiZDB-GENE-051030-97. gamt.

Phylogenomic databases

eggNOGiENOG410ISNM. Eukaryota.
ENOG410XPX4. LUCA.
GeneTreeiENSGT00390000018061.
HOGENOMiHOG000010290.
HOVERGENiHBG005801.
InParanoidiQ71N41.
KOiK00542.
OMAiRYYAFPQ.
OrthoDBiEOG75QR4S.
PhylomeDBiQ71N41.
TreeFamiTF328555.

Enzyme and pathway databases

UniPathwayiUPA00104; UER00580.
ReactomeiR-DRE-71288. Creatine metabolism.

Miscellaneous databases

NextBioi20932772.
PROiQ71N41.

Gene expression databases

BgeeiQ71N41.
ExpressionAtlasiQ71N41. differential.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR016550. GuanidinoAc_N-MeTrfase.
IPR026480. RMT2_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PIRSFiPIRSF009285. GAMT. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51559. SAM_RMT2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of zebrafish guanidinoacetate N-methyltransferase (GAMT) gene."
    Hsiao C.D., Tsai W.Y., Tsai H.J.
    Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: AB.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: AB.
    Tissue: Liver.

Entry informationi

Entry nameiGAMT_DANRE
AccessioniPrimary (citable) accession number: Q71N41
Secondary accession number(s): Q3B7H3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 21, 2006
Last modified: May 11, 2016
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.