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Protein

Chondroitin sulfate proteoglycan 5

Gene

Cspg5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Growth regulation, Neurogenesis

Enzyme and pathway databases

ReactomeiR-MMU-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.
R-MMU-2022870. Chondroitin sulfate biosynthesis.
R-MMU-2022923. Dermatan sulfate biosynthesis.
R-MMU-2024101. CS/DS degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Chondroitin sulfate proteoglycan 5
Alternative name(s):
Acidic leucine-rich EGF-like domain-containing brain protein
Neuroglycan C
Gene namesi
Name:Cspg5
Synonyms:Caleb, Ngc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1352747. Cspg5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini31 – 423ExtracellularSequence analysisAdd BLAST393
Transmembranei424 – 444HelicalSequence analysisAdd BLAST21
Topological domaini445 – 566CytoplasmicSequence analysisAdd BLAST122

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Altered synaptic transmission at early developmental stages.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi38S → A: No effect on chondroitin sulfate attachment. 1 Publication1
Mutagenesisi123S → A: No chondroitin sulfate attachment. no effect on transport to the cell surface. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000004215231 – 566Chondroitin sulfate proteoglycan 5Add BLAST536

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi57N-linked (GlcNAc...)Sequence analysis1
Glycosylationi76O-linked (GalNAc...)Sequence analysis1
Glycosylationi123O-linked (Xyl...) (chondroitin sulfate)1 Publication1
Glycosylationi132O-linked (GalNAc...)Sequence analysis1
Glycosylationi143O-linked (GalNAc...)Sequence analysis1
Glycosylationi144O-linked (GalNAc...)Sequence analysis1
Glycosylationi153O-linked (GalNAc...)Sequence analysis1
Glycosylationi155O-linked (GalNAc...)Sequence analysis1
Glycosylationi156O-linked (GalNAc...)Sequence analysis1
Glycosylationi160O-linked (GalNAc...)Sequence analysis1
Glycosylationi235O-linked (GalNAc...)Sequence analysis1
Glycosylationi355N-linked (GlcNAc...)Sequence analysis1
Glycosylationi367N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi374 ↔ 387By similarity
Disulfide bondi381 ↔ 397By similarity
Disulfide bondi399 ↔ 412By similarity
Modified residuei467PhosphoserineCombined sources1
Modified residuei475PhosphoserineCombined sources1
Modified residuei483PhosphoserineCombined sources1
Modified residuei543PhosphoserineCombined sources1
Isoform 3 (identifier: Q71M36-3)
Modified residuei394PhosphoserineCombined sources1
Modified residuei396PhosphoserineCombined sources1
Modified residuei397PhosphothreonineCombined sources1
Isoform 2 (identifier: Q71M36-2)
Modified residuei475PhosphoserineCombined sources1
Modified residuei477PhosphoserineCombined sources1
Modified residuei478PhosphothreonineCombined sources1

Post-translational modificationi

N-glycosylated.By similarity
O-glycosylated; contains chondroitin sulfate glycans. Part-time proteoglycan, expressed in part as a proteoglycan exhibiting chondroitin sulfate glycans and in part as a non-proteoglycan form. The relative amount of both forms depends on tissues and tissues maturation. In the cerebellum the 2 forms coexist while in the cerebrum the proteoglycan form is predominant.2 Publications
Phosphorylated; in intracellular and extracellular parts.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Proteoglycan

Proteomic databases

PaxDbiQ71M36.
PeptideAtlasiQ71M36.
PRIDEiQ71M36.

PTM databases

iPTMnetiQ71M36.
PhosphoSitePlusiQ71M36.

Expressioni

Tissue specificityi

Expressed in olfactory bulb, hippocampus, brain stem, spinal cord, cerebrum and cerebellum. Expressed by Purkinje cells in the cerebellum (at protein level). Expressed in immature and mature cerebellum (isoform 1, isoform 2 and isoform 3).3 Publications

Developmental stagei

The proteoglycan form decreases from birth to adulthood in the cerebellum concomitant with non-proteoglycan form increase. In the cerebrum the maximum of expression of the proteoglycan is detected 15 days after birth and then decreases gradually to reach half-level at adulthood (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000032482.
CleanExiMM_CSPG5.
GenevisibleiQ71M36. MM.

Interactioni

Subunit structurei

Binds TNR and probably TNC (By similarity). Interacts with ERBB3 and GOPC.By similarity1 Publication

Protein-protein interaction databases

IntActiQ71M36. 1 interactor.
STRINGi10090.ENSMUSP00000035058.

Structurei

3D structure databases

ProteinModelPortaliQ71M36.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini371 – 413EGF-likeAdd BLAST43

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni265 – 301Interaction with TNC and TNR1 PublicationAdd BLAST37
Regioni442 – 460Interaction with GOPCBy similarityAdd BLAST19

Sequence similaritiesi

Contains 1 EGF-like domain.Curated

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJTR. Eukaryota.
ENOG410YF9F. LUCA.
GeneTreeiENSGT00440000034270.
HOGENOMiHOG000112020.
HOVERGENiHBG081361.
InParanoidiQ71M36.
KOiK08116.
OMAiEPTYPFQ.
OrthoDBiEOG091G05S8.
TreeFamiTF338636.

Family and domain databases

InterProiIPR010555. Chon_Sulph_att.
IPR009505. Neural_ProG_Cyt.
[Graphical view]
PfamiPF06566. Chon_Sulph_att. 1 hit.
PF06567. Neural_ProG_Cyt. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q71M36-1) [UniParc]FASTAAdd to basket
Also known as: NGC-III

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRAGGGGPD WGPPPVLLLL GVTLVLTAGA VPARETGSAI EAEELVRSSL
60 70 80 90 100
AWESRANDTR EEAGLPAAGE DETSWTERGS EMAAVGPGVG PEEALEASAA
110 120 130 140 150
VTGTAWLEAD GPGLGGVTAE AGSGDAQTLP ATLQAPDEAL GSSTMPPAIP
160 170 180 190 200
EATETSGPPS PAVHDKPSVG PELPKEIPLE VRLNLGGSTP EPTFPLQGTL
210 220 230 240 250
ETQPASDIID IDYFEGLDSE GRGADMGSFP GSPGTSENHP DTEGETPSWS
260 270 280 290 300
LLDLYDDFTP FDESDFYPTT SFYDDLEEEE EEEEDKDTVG GGDLEDENDL
310 320 330 340 350
LLPSQKPGVG PGTGQPTNRW HAVPPQHTLG MVPGSSISLR PRPGDPGKDL
360 370 380 390 400
ASGENGTECR VGFVRHNGSC RSVCDLFPSY CHNGGQCYLV ENIGAFCRCN
410 420 430 440 450
TQDYIWHKGM RCESIITDFQ VMCVAVGSAA LVLLLLFMMT VFFAKKLYLL
460 470 480 490 500
KTENTKLRRT NKFRTPSELH NDNFSLSTIA EGSHPNVRKF CDTPRVSSPH
510 520 530 540 550
ARALAHYDNI VCQDDPSAPH KIQDPLKSRL KEEESFNIQN SMSPKLEGGK
560
GDQDDLGVNC LQNNLT
Length:566
Mass (Da):60,406
Last modified:July 27, 2011 - v2
Checksum:i8AB75BA1ACB11BDE
GO
Isoform 2 (identifier: Q71M36-2) [UniParc]FASTAAdd to basket
Also known as: NGC-I

The sequence of this isoform differs from the canonical sequence as follows:
     487-513: Missing.

Note: Major isoform.Combined sources
Show »
Length:539
Mass (Da):57,339
Checksum:i81B105D7803930A3
GO
Isoform 3 (identifier: Q71M36-3) [UniParc]FASTAAdd to basket
Also known as: NGC-II

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: Missing.
     487-513: Missing.

Show »
Length:458
Mass (Da):49,027
Checksum:i87CE103CCD23205E
GO
Isoform 4 (identifier: Q71M36-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     514-566: DDPSAPHKIQ...GVNCLQNNLT → VTYLPHISPF...RERCIDSLTV

Note: No experimental confirmation available.
Show »
Length:569
Mass (Da):60,857
Checksum:i62DADBBC033BF0E0
GO

Sequence cautioni

The sequence AAQ04778 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC35578 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti317T → N in AAF23362 (PubMed:10617623).Curated1
Sequence conflicti317T → N in AAQ04777 (PubMed:10617623).Curated1
Sequence conflicti317T → N in AAQ04778 (PubMed:10617623).Curated1
Sequence conflicti317T → N in AAQ04779 (PubMed:10617623).Curated1
Sequence conflicti318N → H in AAH55736 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0157621 – 81Missing in isoform 3. 1 PublicationAdd BLAST81
Alternative sequenceiVSP_015763487 – 513Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST27
Alternative sequenceiVSP_015764514 – 566DDPSA…QNNLT → VTYLPHISPFACLCPCLPLP PCPLALSQSRQSPNSFEDQL RATQWCRERCIDSLTV in isoform 4. 1 PublicationAdd BLAST53

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF133700 mRNA. Translation: AAF23362.1.
AF461090 mRNA. Translation: AAQ04777.1.
AF461091 mRNA. Translation: AAQ04778.1. Different initiation.
AF461092 mRNA. Translation: AAQ04779.1.
AC159372 Genomic DNA. No translation available.
AC160104 Genomic DNA. No translation available.
BC055736 mRNA. Translation: AAH55736.1.
AK053891 mRNA. Translation: BAC35578.1. Different initiation.
CCDSiCCDS52936.1. [Q71M36-1]
CCDS81085.1. [Q71M36-2]
RefSeqiNP_001159745.1. NM_001166273.1. [Q71M36-1]
NP_038912.3. NM_013884.3. [Q71M36-2]
UniGeneiMm.38496.

Genome annotation databases

EnsembliENSMUST00000035058; ENSMUSP00000035058; ENSMUSG00000032482. [Q71M36-1]
ENSMUST00000196060; ENSMUSP00000143164; ENSMUSG00000032482. [Q71M36-2]
ENSMUST00000197850; ENSMUSP00000143005; ENSMUSG00000032482. [Q71M36-4]
ENSMUST00000199736; ENSMUSP00000142845; ENSMUSG00000032482. [Q71M36-3]
GeneIDi29873.
KEGGimmu:29873.
UCSCiuc009rtq.2. mouse. [Q71M36-1]
uc009rtr.2. mouse. [Q71M36-2]
uc009rtt.1. mouse. [Q71M36-3]
uc009rtu.1. mouse. [Q71M36-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF133700 mRNA. Translation: AAF23362.1.
AF461090 mRNA. Translation: AAQ04777.1.
AF461091 mRNA. Translation: AAQ04778.1. Different initiation.
AF461092 mRNA. Translation: AAQ04779.1.
AC159372 Genomic DNA. No translation available.
AC160104 Genomic DNA. No translation available.
BC055736 mRNA. Translation: AAH55736.1.
AK053891 mRNA. Translation: BAC35578.1. Different initiation.
CCDSiCCDS52936.1. [Q71M36-1]
CCDS81085.1. [Q71M36-2]
RefSeqiNP_001159745.1. NM_001166273.1. [Q71M36-1]
NP_038912.3. NM_013884.3. [Q71M36-2]
UniGeneiMm.38496.

3D structure databases

ProteinModelPortaliQ71M36.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ71M36. 1 interactor.
STRINGi10090.ENSMUSP00000035058.

PTM databases

iPTMnetiQ71M36.
PhosphoSitePlusiQ71M36.

Proteomic databases

PaxDbiQ71M36.
PeptideAtlasiQ71M36.
PRIDEiQ71M36.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035058; ENSMUSP00000035058; ENSMUSG00000032482. [Q71M36-1]
ENSMUST00000196060; ENSMUSP00000143164; ENSMUSG00000032482. [Q71M36-2]
ENSMUST00000197850; ENSMUSP00000143005; ENSMUSG00000032482. [Q71M36-4]
ENSMUST00000199736; ENSMUSP00000142845; ENSMUSG00000032482. [Q71M36-3]
GeneIDi29873.
KEGGimmu:29873.
UCSCiuc009rtq.2. mouse. [Q71M36-1]
uc009rtr.2. mouse. [Q71M36-2]
uc009rtt.1. mouse. [Q71M36-3]
uc009rtu.1. mouse. [Q71M36-4]

Organism-specific databases

CTDi10675.
MGIiMGI:1352747. Cspg5.

Phylogenomic databases

eggNOGiENOG410IJTR. Eukaryota.
ENOG410YF9F. LUCA.
GeneTreeiENSGT00440000034270.
HOGENOMiHOG000112020.
HOVERGENiHBG081361.
InParanoidiQ71M36.
KOiK08116.
OMAiEPTYPFQ.
OrthoDBiEOG091G05S8.
TreeFamiTF338636.

Enzyme and pathway databases

ReactomeiR-MMU-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.
R-MMU-2022870. Chondroitin sulfate biosynthesis.
R-MMU-2022923. Dermatan sulfate biosynthesis.
R-MMU-2024101. CS/DS degradation.

Miscellaneous databases

ChiTaRSiCspg5. mouse.
PROiQ71M36.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032482.
CleanExiMM_CSPG5.
GenevisibleiQ71M36. MM.

Family and domain databases

InterProiIPR010555. Chon_Sulph_att.
IPR009505. Neural_ProG_Cyt.
[Graphical view]
PfamiPF06566. Chon_Sulph_att. 1 hit.
PF06567. Neural_ProG_Cyt. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCSPG5_MOUSE
AccessioniPrimary (citable) accession number: Q71M36
Secondary accession number(s): E9QN54
, Q71M37, Q7TNT8, Q8BPJ5, Q9QY32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Different forms of various molecular weight have been observed. Such forms are possibly due to different levels of glycosylation, phosphorylation and/or protein cleavage.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.