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Protein

Delta(14)-sterol reductase

Gene

Tm7sf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the conversion of lanosterol to cholesterol.1 Publication

Catalytic activityi

4,4-dimethyl-5-alpha-cholesta-8,24-dien-3-beta-ol + NADP+ = 4,4-dimethyl-5-alpha-cholesta-8,14,24-trien-3-beta-ol + NADPH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei311NADPBy similarity1
Binding sitei315NADPBy similarity1
Binding sitei338NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei343NADPBy similarity1
Binding sitei390NADPBy similarity1
Binding sitei405NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi350 – 351NADPBy similarity2
Nucleotide bindingi394 – 398NADPBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-MMU-191273. Cholesterol biosynthesis.
R-MMU-2426168. Activation of gene expression by SREBF (SREBP).

Names & Taxonomyi

Protein namesi
Recommended name:
Delta(14)-sterol reductase (EC:1.3.1.70)
Short name:
Delta-14-SR
Alternative name(s):
C-14 sterol reductase
Short name:
C14SR
Sterol C14-reductase
Transmembrane 7 superfamily member 2
Gene namesi
Name:Tm7sf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1920416. Tm7sf2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei13 – 35HelicalSequence analysisAdd BLAST23
Transmembranei62 – 81HelicalSequence analysisAdd BLAST20
Transmembranei102 – 124HelicalSequence analysisAdd BLAST23
Transmembranei129 – 148HelicalSequence analysisAdd BLAST20
Transmembranei255 – 277HelicalSequence analysisAdd BLAST23
Transmembranei287 – 304HelicalSequence analysisAdd BLAST18
Transmembranei355 – 377HelicalSequence analysisAdd BLAST23

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Disruption phenotypei

mice develop normally, appear healthy and are fertile.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003311211 – 418Delta(14)-sterol reductaseAdd BLAST418

Proteomic databases

MaxQBiQ71KT5.
PaxDbiQ71KT5.
PeptideAtlasiQ71KT5.
PRIDEiQ71KT5.

PTM databases

iPTMnetiQ71KT5.
PhosphoSitePlusiQ71KT5.

Expressioni

Tissue specificityi

Strongly expressed in liver, weaker in ovary, testis, kidney and brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000024799.
ExpressionAtlasiQ71KT5. baseline and differential.
GenevisibleiQ71KT5. MM.

Interactioni

Protein-protein interaction databases

MINTiMINT-1857062.
STRINGi10090.ENSMUSP00000025713.

Structurei

3D structure databases

ProteinModelPortaliQ71KT5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ERG4/ERG24 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1435. Eukaryota.
ENOG410XP67. LUCA.
GeneTreeiENSGT00390000000417.
HOGENOMiHOG000193296.
HOVERGENiHBG007825.
InParanoidiQ71KT5.
KOiK00222.
OMAiLPCGLSH.
OrthoDBiEOG091G0F22.
TreeFamiTF101179.

Family and domain databases

InterProiIPR001171. Ergosterol_biosynth_ERG4_ERG24.
IPR018083. Sterol_reductase_CS.
[Graphical view]
PfamiPF01222. ERG4_ERG24. 1 hit.
[Graphical view]
PROSITEiPS01017. STEROL_REDUCT_1. 1 hit.
PS01018. STEROL_REDUCT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q71KT5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSREASQAP LEFGGPLGVA ALLILLPATM FHLLLAARSG PARLLALPAY
60 70 80 90 100
LPGLEELWSP WALLLLFIWL GLQVALYLLP ARKVAEGLEL KDKSRLRYPI
110 120 130 140 150
NGFQALVLTA LLMGLGVSVG LPLGALPGML LPLAFATTLT SFIFSLLLYA
160 170 180 190 200
KALVAPASAL APGGNSGNSM YDFFLGRELN PRLGSFDFKY FCELRPGLIG
210 220 230 240 250
WVFINLALLM QEAELRGSPS LAMWLVNGFQ LLYVGDALWY EESVLTTMDI
260 270 280 290 300
IHDGFGFMLV FGDLAWVPFT YSLQAQFLLY HPQPLGLPMA LLICLLKVIG
310 320 330 340 350
YYIFRGANSQ KNTFRKNPSD PSVAGLETIP TATGRQLLVS GWWGMVRHPN
360 370 380 390 400
YLGDLIMALA WSLPCGLSHL LPYFYVLYFT ALLVHREARD EQQCLQKYGR
410
AWQEYCKRVP YRIIPYVY
Length:418
Mass (Da):46,521
Last modified:July 27, 2011 - v2
Checksum:i0DCCA4E4B9423A31
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti298V → A in AAQ05836 (Ref. 1) Curated1
Sequence conflicti298V → A in BAE33285 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF480070 mRNA. Translation: AAQ05836.1.
EU672836 Genomic DNA. Translation: ACF94776.1.
AK155475 mRNA. Translation: BAE33285.1.
CH466612 Genomic DNA. Translation: EDL33202.1.
CCDSiCCDS29490.2.
RefSeqiNP_082730.2. NM_028454.2.
UniGeneiMm.489613.

Genome annotation databases

EnsembliENSMUST00000025713; ENSMUSP00000025713; ENSMUSG00000024799.
GeneIDi73166.
KEGGimmu:73166.
UCSCiuc008ggw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF480070 mRNA. Translation: AAQ05836.1.
EU672836 Genomic DNA. Translation: ACF94776.1.
AK155475 mRNA. Translation: BAE33285.1.
CH466612 Genomic DNA. Translation: EDL33202.1.
CCDSiCCDS29490.2.
RefSeqiNP_082730.2. NM_028454.2.
UniGeneiMm.489613.

3D structure databases

ProteinModelPortaliQ71KT5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1857062.
STRINGi10090.ENSMUSP00000025713.

PTM databases

iPTMnetiQ71KT5.
PhosphoSitePlusiQ71KT5.

Proteomic databases

MaxQBiQ71KT5.
PaxDbiQ71KT5.
PeptideAtlasiQ71KT5.
PRIDEiQ71KT5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025713; ENSMUSP00000025713; ENSMUSG00000024799.
GeneIDi73166.
KEGGimmu:73166.
UCSCiuc008ggw.1. mouse.

Organism-specific databases

CTDi7108.
MGIiMGI:1920416. Tm7sf2.

Phylogenomic databases

eggNOGiKOG1435. Eukaryota.
ENOG410XP67. LUCA.
GeneTreeiENSGT00390000000417.
HOGENOMiHOG000193296.
HOVERGENiHBG007825.
InParanoidiQ71KT5.
KOiK00222.
OMAiLPCGLSH.
OrthoDBiEOG091G0F22.
TreeFamiTF101179.

Enzyme and pathway databases

ReactomeiR-MMU-191273. Cholesterol biosynthesis.
R-MMU-2426168. Activation of gene expression by SREBF (SREBP).

Miscellaneous databases

PROiQ71KT5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024799.
ExpressionAtlasiQ71KT5. baseline and differential.
GenevisibleiQ71KT5. MM.

Family and domain databases

InterProiIPR001171. Ergosterol_biosynth_ERG4_ERG24.
IPR018083. Sterol_reductase_CS.
[Graphical view]
PfamiPF01222. ERG4_ERG24. 1 hit.
[Graphical view]
PROSITEiPS01017. STEROL_REDUCT_1. 1 hit.
PS01018. STEROL_REDUCT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERG24_MOUSE
AccessioniPrimary (citable) accession number: Q71KT5
Secondary accession number(s): B5LBK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.