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Protein

E3 ubiquitin-protein ligase SH3RF1

Gene

Sh3rf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7, MAP2K7 and JNKs (MAPK8 and MAPK9). In neuronal cells, it induces apoptosis.1 Publication
Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes such as UBE2D1 or UBE2N and then transfers it to substrates. In the absence of an external substrate, it can catalyze self-ubiquitination. Stimulates ubiquitination of potassium channel KCNJ1, enhancing it's dynamin-dependent and clathrin-independent endocytosis.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri12 – 53RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase SH3RF1 (EC:2.3.2.27)
Alternative name(s):
Plenty of SH3s
Short name:
Protein POSH
RING-type E3 ubiquitin transferase SH3RF1Curated
SH3 domain-containing RING finger protein 1
SH3 multiple domains protein 2
Gene namesi
Name:Sh3rf1
Synonyms:Posh, Sh3md2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi735154 Sh3rf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003341541 – 894E3 ubiquitin-protein ligase SH3RF1Add BLAST894

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei305PhosphoserineBy similarity1
Modified residuei540PhosphoserineBy similarity1
Modified residuei743PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Ser-305 by AKT1 and AKT2. When phosphorylated, it has reduced ability to bind Rac.By similarity
Subject to ubiquitination and proteasomal degradation.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ71F54
PRIDEiQ71F54

PTM databases

iPTMnetiQ71F54
PhosphoSitePlusiQ71F54

Expressioni

Gene expression databases

BgeeiENSRNOG00000010358
GenevisibleiQ71F54 RN

Interactioni

Subunit structurei

Interacts with HERP1 (By similarity). Interacts with Rac; in a GTP-dependent manner. Interacts with MAP3K10 and MAP3K11. Interacts with MAPK8IP; this interaction leads to the PJAC complex (POSH-JIP or SH3RF1/MAPK8IP apoptotic complex) with a 1:1 ratio. Interacts with SIAH1. Probably part of a signaling complex that may contain SH3RF1, MAPK8IP, DLK1, MAP2K4, MAP2K7, MAPK8, MAPK9, AKT1 and AKT2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Siah1Q920M92EBI-957526,EBI-957514

Protein-protein interaction databases

IntActiQ71F54, 1 interactor
STRINGi10116.ENSRNOP00000013815

Structurei

3D structure databases

ProteinModelPortaliQ71F54
SMRiQ71F54
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini134 – 193SH3 1PROSITE-ProRule annotationAdd BLAST60
Domaini196 – 259SH3 2PROSITE-ProRule annotationAdd BLAST64
Domaini453 – 514SH3 3PROSITE-ProRule annotationAdd BLAST62
Domaini835 – 894SH3 4PROSITE-ProRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni448 – 551Interaction with AKT2By similarityAdd BLAST104

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi84 – 89Poly-Gly6
Compositional biasi398 – 404Poly-Pro7
Compositional biasi418 – 432Ala-richAdd BLAST15

Domaini

The RING finger domain is responsible of ubiquitination and proteasomal degradation.

Sequence similaritiesi

Belongs to the SH3RF family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri12 – 53RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Repeat, SH3 domain, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITG6 Eukaryota
ENOG410XS5R LUCA
GeneTreeiENSGT00760000119190
HOGENOMiHOG000045564
HOVERGENiHBG069552
InParanoidiQ71F54
KOiK12171
OMAiGWYKGTH
OrthoDBiEOG091G09B6
PhylomeDBiQ71F54
TreeFamiTF105571

Family and domain databases

CDDicd11930 SH3_SH3RF1_2, 1 hit
cd11785 SH3_SH3RF_C, 1 hit
Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR028502 SH3RF1
IPR035816 SH3RF1/SH3RF3_SH3_4
IPR035795 SH3RF1_SH3_2
IPR018957 Znf_C3HC4_RING-type
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR44208:SF2 PTHR44208:SF2, 1 hit
PfamiView protein in Pfam
PF00018 SH3_1, 1 hit
PF14604 SH3_9, 3 hits
PF00097 zf-C3HC4, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00184 RING, 1 hit
SM00326 SH3, 4 hits
SUPFAMiSSF50044 SSF50044, 4 hits
PROSITEiView protein in PROSITE
PS50002 SH3, 4 hits
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q71F54-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDESALLDLL ECPVCLERLD ASAKVLPCQH TFCKRCLLGI VGSRNELRCP
60 70 80 90 100
ECRTLVGSGV DELPSNILLV RLLDGIKQRP WKPGPGGGGS TTCTNVLRAQ
110 120 130 140 150
GSTVVNCGSK DLQSPQCGQQ PRVQAWSPPV RGIPQLPCAK ALYNYEGKEP
160 170 180 190 200
GDLKFSKGDI IILRRQVDEN WYHGEVNGVH GFFPTNFVQI IKPLPQPPPQ
210 220 230 240 250
CKALYDFEVK DKEADKDCLP FAKDDVLTVI RRVDENWAEG MLADKIGIFP
260 270 280 290 300
ISYVEFNSAA KQLIEWDKPP VPGVDTAECP SATAAQSSSA SKHSDTKKNT
310 320 330 340 350
RKRHSFTSLT MANKSSQASQ NRHSMEISPP VLISSSNPTA AARISELSGL
360 370 380 390 400
SCSAPSQVHI STTGLIVTPP PSSPVTTGPS FTFPTDVPYQ AALGTMNPPL
410 420 430 440 450
PPPPLLATTV LASTPSGATA AAVAAAAAAV AAGVGPRPAV GSTEQIAHLR
460 470 480 490 500
PQTRPSVYVA IYPYTPRKED ELELRKGEMF LVFERCQDGW YKGTSMHTSK
510 520 530 540 550
IGVFPGNYVA PVTRAVTNAS QAKVPMSTAG QASRGVTMVS PSTAGGPAQK
560 570 580 590 600
PQGNGVAGNP SVVPTAVVSA AHIQTSPQAK VLLHMTGQMT VNQARNAVRT
610 620 630 640 650
VAAHNQERPT AAVTPIQVQN AACIGPASVG LPHHSLASQP LPPMVGPAAH
660 670 680 690 700
IAAVNINRTS VPLACAAGAS SLASPNMTTA ALETEPSGRT VTILPGLPTS
710 720 730 740 750
PESAASACGN SSAVKPDKDS KKEKKGLLKL LSGASTKRKP RVSPPASPTL
760 770 780 790 800
DVELGSGEVP LQGAVGPELP LGGVHGRVGS CPTDGDGPVA AGTAALAQDA
810 820 830 840 850
FHRKTSSLDS AVPIAPPPRQ ACSSLGPVMN EARPVVCERH RVVVSYPPQS
860 870 880 890
EAELELKEGD IVFVHKKRED GWFKGTLQRN GKTGLFPGSF VENI
Length:894
Mass (Da):93,879
Last modified:July 5, 2004 - v1
Checksum:iE7CB1B7E6330C62D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF515735 mRNA Translation: AAQ08184.1
RefSeqiNP_942059.1, NM_198764.1
XP_017455595.1, XM_017600106.1
UniGeneiRn.163325

Genome annotation databases

EnsembliENSRNOT00000013816; ENSRNOP00000013815; ENSRNOG00000010358
GeneIDi306417
KEGGirno:306417
UCSCiRGD:735154 rat

Entry informationi

Entry nameiSH3R1_RAT
AccessioniPrimary (citable) accession number: Q71F54
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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