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Protein

E3 ubiquitin-protein ligase SH3RF1

Gene

Sh3rf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7, MAP2K7 and JNKs (MAPK8 and MAPK9). In neuronal cells, it induces apoptosis.1 Publication
Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes such as UBE2D1 or UBE2N and then transfers it to substrates. In the absence of an external substrate, it can catalyze self-ubiquitination. Stimulates ubiquitination of potassium channel KCNJ1, enhancing it's dynamin-dependent and clathrin-independent endocytosis (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri12 – 5342RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase SH3RF1 (EC:6.3.2.-)
Alternative name(s):
Plenty of SH3s
Short name:
Protein POSH
SH3 domain-containing RING finger protein 1
SH3 multiple domains protein 2
Gene namesi
Name:Sh3rf1
Synonyms:Posh, Sh3md2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi735154. Sh3rf1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 894894E3 ubiquitin-protein ligase SH3RF1PRO_0000334154Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei305 – 3051PhosphoserineBy similarity
Modified residuei540 – 5401PhosphoserineBy similarity
Modified residuei743 – 7431PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated at Ser-305 by AKT1 and AKT2. When phosphorylated, it has reduced ability to bind Rac (By similarity).By similarity
Subject to ubiquitination and proteasomal degradation.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ71F54.

PTM databases

iPTMnetiQ71F54.
PhosphoSiteiQ71F54.

Expressioni

Gene expression databases

GenevisibleiQ71F54. RN.

Interactioni

Subunit structurei

Interacts with HERP1 (By similarity). Interacts with Rac; in a GTP-dependent manner. Interacts with MAP3K10 and MAP3K11. Interacts with MAPK8IP; this interaction leads to the PJAC complex (POSH-JIP or SH3RF1/MAPK8IP apoptotic complex) with a 1:1 ratio. Interacts with SIAH1. Probably part of a signaling complex that may contain SH3RF1, MAPK8IP, DLK1, MAP2K4, MAP2K7, MAPK8, MAPK9, AKT1 and AKT2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Siah1Q920M92EBI-957526,EBI-957514

Protein-protein interaction databases

IntActiQ71F54. 1 interaction.
STRINGi10116.ENSRNOP00000013815.

Structurei

3D structure databases

ProteinModelPortaliQ71F54.
SMRiQ71F54. Positions 137-191.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini134 – 19360SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini196 – 25964SH3 2PROSITE-ProRule annotationAdd
BLAST
Domaini453 – 51462SH3 3PROSITE-ProRule annotationAdd
BLAST
Domaini835 – 89460SH3 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni448 – 551104Interaction with AKT2By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi84 – 896Poly-Gly
Compositional biasi398 – 4047Poly-Pro
Compositional biasi418 – 43215Ala-richAdd
BLAST

Domaini

The RING finger domain is responsible of ubiquitination and proteasomal degradation.

Sequence similaritiesi

Belongs to the SH3RF family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 4 SH3 domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri12 – 5342RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, SH3 domain, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITG6. Eukaryota.
ENOG410XS5R. LUCA.
GeneTreeiENSGT00760000119190.
HOGENOMiHOG000045564.
HOVERGENiHBG069552.
InParanoidiQ71F54.
KOiK12171.
OMAiCTNALRA.
OrthoDBiEOG7HMS0X.
PhylomeDBiQ71F54.
TreeFamiTF105571.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR028502. POSH1.
IPR001452. SH3_domain.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR10663:SF153. PTHR10663:SF153. 5 hits.
PfamiPF00018. SH3_1. 1 hit.
PF14604. SH3_9. 3 hits.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00184. RING. 1 hit.
SM00326. SH3. 4 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 4 hits.
PROSITEiPS50002. SH3. 4 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q71F54-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDESALLDLL ECPVCLERLD ASAKVLPCQH TFCKRCLLGI VGSRNELRCP
60 70 80 90 100
ECRTLVGSGV DELPSNILLV RLLDGIKQRP WKPGPGGGGS TTCTNVLRAQ
110 120 130 140 150
GSTVVNCGSK DLQSPQCGQQ PRVQAWSPPV RGIPQLPCAK ALYNYEGKEP
160 170 180 190 200
GDLKFSKGDI IILRRQVDEN WYHGEVNGVH GFFPTNFVQI IKPLPQPPPQ
210 220 230 240 250
CKALYDFEVK DKEADKDCLP FAKDDVLTVI RRVDENWAEG MLADKIGIFP
260 270 280 290 300
ISYVEFNSAA KQLIEWDKPP VPGVDTAECP SATAAQSSSA SKHSDTKKNT
310 320 330 340 350
RKRHSFTSLT MANKSSQASQ NRHSMEISPP VLISSSNPTA AARISELSGL
360 370 380 390 400
SCSAPSQVHI STTGLIVTPP PSSPVTTGPS FTFPTDVPYQ AALGTMNPPL
410 420 430 440 450
PPPPLLATTV LASTPSGATA AAVAAAAAAV AAGVGPRPAV GSTEQIAHLR
460 470 480 490 500
PQTRPSVYVA IYPYTPRKED ELELRKGEMF LVFERCQDGW YKGTSMHTSK
510 520 530 540 550
IGVFPGNYVA PVTRAVTNAS QAKVPMSTAG QASRGVTMVS PSTAGGPAQK
560 570 580 590 600
PQGNGVAGNP SVVPTAVVSA AHIQTSPQAK VLLHMTGQMT VNQARNAVRT
610 620 630 640 650
VAAHNQERPT AAVTPIQVQN AACIGPASVG LPHHSLASQP LPPMVGPAAH
660 670 680 690 700
IAAVNINRTS VPLACAAGAS SLASPNMTTA ALETEPSGRT VTILPGLPTS
710 720 730 740 750
PESAASACGN SSAVKPDKDS KKEKKGLLKL LSGASTKRKP RVSPPASPTL
760 770 780 790 800
DVELGSGEVP LQGAVGPELP LGGVHGRVGS CPTDGDGPVA AGTAALAQDA
810 820 830 840 850
FHRKTSSLDS AVPIAPPPRQ ACSSLGPVMN EARPVVCERH RVVVSYPPQS
860 870 880 890
EAELELKEGD IVFVHKKRED GWFKGTLQRN GKTGLFPGSF VENI
Length:894
Mass (Da):93,879
Last modified:July 5, 2004 - v1
Checksum:iE7CB1B7E6330C62D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF515735 mRNA. Translation: AAQ08184.1.
RefSeqiNP_942059.1. NM_198764.1.
UniGeneiRn.163325.

Genome annotation databases

EnsembliENSRNOT00000013816; ENSRNOP00000013815; ENSRNOG00000010358.
GeneIDi306417.
KEGGirno:306417.
UCSCiRGD:735154. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF515735 mRNA. Translation: AAQ08184.1.
RefSeqiNP_942059.1. NM_198764.1.
UniGeneiRn.163325.

3D structure databases

ProteinModelPortaliQ71F54.
SMRiQ71F54. Positions 137-191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ71F54. 1 interaction.
STRINGi10116.ENSRNOP00000013815.

PTM databases

iPTMnetiQ71F54.
PhosphoSiteiQ71F54.

Proteomic databases

PaxDbiQ71F54.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013816; ENSRNOP00000013815; ENSRNOG00000010358.
GeneIDi306417.
KEGGirno:306417.
UCSCiRGD:735154. rat.

Organism-specific databases

CTDi57630.
RGDi735154. Sh3rf1.

Phylogenomic databases

eggNOGiENOG410ITG6. Eukaryota.
ENOG410XS5R. LUCA.
GeneTreeiENSGT00760000119190.
HOGENOMiHOG000045564.
HOVERGENiHBG069552.
InParanoidiQ71F54.
KOiK12171.
OMAiCTNALRA.
OrthoDBiEOG7HMS0X.
PhylomeDBiQ71F54.
TreeFamiTF105571.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ71F54.

Gene expression databases

GenevisibleiQ71F54. RN.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR028502. POSH1.
IPR001452. SH3_domain.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR10663:SF153. PTHR10663:SF153. 5 hits.
PfamiPF00018. SH3_1. 1 hit.
PF14604. SH3_9. 3 hits.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00184. RING. 1 hit.
SM00326. SH3. 4 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 4 hits.
PROSITEiPS50002. SH3. 4 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "POSH acts as a scaffold for a multiprotein complex that mediates JNK activation in apoptosis."
    Xu Z., Kukekov N.V., Greene L.A.
    EMBO J. 22:252-261(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH MAP3K10; MAP3K11; DLK1; MAP2K4; MAP2K7; MAPK8 AND MAPK9, UBIQUITINATION, FUNCTION.
    Strain: New England Deaconess Hospital.
  2. "Siah1 interacts with the scaffold protein POSH to promote JNK activation and apoptosis."
    Xu Z., Sproul A., Wang W., Kukekov N., Greene L.A.
    J. Biol. Chem. 281:303-312(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SIAH1.
  3. "Direct interaction of the molecular scaffolds POSH and JIP is required for apoptotic activation of JNKs."
    Kukekov N.V., Xu Z., Greene L.A.
    J. Biol. Chem. 281:15517-15524(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAPK8IP, SUBCELLULAR LOCATION.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-743, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSH3R1_RAT
AccessioniPrimary (citable) accession number: Q71F54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.