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Reviewed, UniProtKB/Swiss-Prot Q71DJ5 (LIP1_ARATH)

Last modified November 3, 2009. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Triacylglycerol lipase 1
    EC=3.1.1.3
Gene names
Name: LIP1
Ordered Locus Names: At2g15230
ORF Names: F15A23.3
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Triacylglycerol (TAG) lipase active on triolein, trioctanoin, tributyrin and 1,3-Diolein, but not on phospho- and galactolipids. May be involved but dispensable for TAG storage breakdown during seed germination. Ref.1

Catalytic activity

Triacylglycerol + H2O = diacylglycerol + a carboxylate.

Subcellular location

Secreted Probable.

Tissue specificity

Expressed in seedlings, roots, leaves, flowers and siliques. Ref.1

Sequence similarities

Belongs to the AB hydrolase superfamily. Lipase family.

Biophysicochemical properties

Kinetic parameters:

Vmax=45 µmol/min/mg enzyme with triolein as a substrate

pH dependence:

Optimum pH is 6. Active between pH 4-7.

Sequence caution

The sequence AAD25569.1 differs from that shown. Reason: Erroneous gene model prediction.

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Ontologies

Keywords
   Biological processLipid degradation
   Cellular componentSecreted
   DomainSignal
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processlipid catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentextracellular space Ref.1

Traceable author statement. Source: TAIR

   Molecular functiontriglyceride lipase activity Ref.1

Inferred from direct assay. Source: TAIR

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Ref.1
Chain21 – 393373Triacylglycerol lipase 1
PRO_0000234336

Sites

Active site1661Nucleophile By similarity
Active site3341Charge relay system By similarity
Active site3631Charge relay system By similarity

Amino acid modifications

Glycosylation411N-linked (GlcNAc...) Potential
Glycosylation2611N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
Q71DJ5-1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: C904556DCB44178D

FASTA39344,231
        10         20         30         40         50         60 
MKWLLVAVLT SLTIFSALTQ SHLLHGSPVN SLCADLIHPA NYSCTEHSIQ TKDGYILALQ 

        70         80         90        100        110        120 
RVASLGPRLQ SGPPVLLQHG LFMAGDVWFL NSPKESLGFI LADHGFDVWV GNVRGTRYSY 

       130        140        150        160        170        180 
GHVTLSDTDK EFWDWSWQDL AMYDLAEMIQ YLYSISNSKI FLVGHSQGTI MSFAALTQPH 

       190        200        210        220        230        240 
VAEMVEAAAL LCPISYLDHV TAPLVERMVF MHLDQMVVAL GLHQINFRSD MLVKLVDSLC 

       250        260        270        280        290        300 
EGHMDCTDFL TSITGTNCCF NASKIEYYLD YEPHPSSVKN IRHLFQMIRK GTFAQYDYGY 

       310        320        330        340        350        360 
FKNLRTYGLS KPPEFILSHI PASLPMWMGY GGTDGLADVT DVEHTLAELP SSPELLYLED 

       370        380        390 
YGHIDFVLGS SAKEDVYKHM IQFFRAKVKS SSW

« Hide

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References

« Hide 'large scale' references
[1]"Identification and characterization of a triacylglycerol lipase in Arabidopsis homologous to mammalian acid lipases."
El-Kouhen K., Blangy S., Ortiz E., Gardies A.-M., Ferte N., Arondel V.
FEBS Lett. 579:6067-6073(2005) [PubMed: 16226259] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-28, FUNCTION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES.
[2]"Triacylglycerol (steryl ester) hydrolase from Arabidopsis thaliana is involved in lipid homeostasy."
Benveniste P., Noiriel A., Nave P., Schaller H.
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.

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Cross-references

Sequence databases

AF530477 mRNA. Translation: AAN77143.1.
AC006298 Genomic DNA. Translation: AAD25569.1. Sequence problems.
IPIIPI00539331.
PIRE84526.
RefSeqNP_179126.2.
UniGeneAt.40439

3D structure databases

HSSPHSSP built from PDB template 1K8Q based on UniProtKB P80035.
ModBaseSearch...

Genome annotation databases

GeneID816012.
GenomeReviewsGene locus AT2G15230 in contig CT485783_GR.
KEGGath:AT2G15230.
NMPDRfig|3702.1.peg.8526.

Organism-specific databases

TAIRAt2g15230.

Phylogenomic databases

OMAPIIERIN.

Enzyme and pathway databases

BRENDA3.1.1.3. 302.

Gene expression databases

GenevestigatorQ71DJ5.
GermOnlineAT2G15230. Arabidopsis thaliana.

Family and domain databases

InterProIPR006693. AB_hydro_lipase.
IPR000073. AB_hydrolase_1.
IPR008262. Lipase_Ser_AS.
[Graphical view]
PfamPF04083. Abhydro_lipase. 1 hit.
PF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PROSITEPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameLIP1_ARATH
AccessionPrimary (citable) accession number: Q71DJ5
Secondary accession number(s): Q9SKL5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: July 5, 2004
Last modified: November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents