Q719N1 (SPAST_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spastin
EC=3.6.4.3

Gene names

Name:	SPAST
Synonyms:	SPG4

Organism

Sus scrofa (Pig) [Complete proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	613 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis. Also plays a role in axon growth and the formation of axonal branches By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Homohexamer. Binding to ATP stabilizes the homohexameric form. Binds to microtubules at least in part via the alpha-tubulin and beta-tubulin tails. The hexamer may adopt a ring conformation through which microtubules pass prior to being severed. Does not interact strongly with tubulin heterodimers. Interacts (via MIT domain) with CHMP1B; the interaction is direct. Interacts with ATL1, RTN1, SSNA1 and ZFYVE27 By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton By similarity. Cytoplasm › perinuclear region By similarity. Endoplasmic reticulum By similarity. Endosome By similarity. Nucleus By similarity. Cytoplasm › cytoskeleton › spindle By similarity. Note: Localization to the centrosome is independent of microtubules. Localizes to the midbody of dividing cells, and this requires CHMP1B. Enriched in the distal axons and branches of postmitotic neurons By similarity.
Sequence similarities	Belongs to the AAA ATPase family. Spastin subfamily. Contains 1 MIT domain.
Sequence caution	The sequence AAQ11224.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Cell division Differentiation Neurogenesis
Cellular component	Cytoplasm Cytoskeleton Endoplasmic reticulum Endosome Membrane Microtubule Nucleus
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from sequence or structural similarity. Source: UniProtKB cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW cytokinesis, completion of separation Inferred from sequence or structural similarity. Source: UniProtKB microtubule bundle formation Inferred from sequence or structural similarity. Source: UniProtKB microtubule severing Inferred from sequence or structural similarity. Source: UniProtKB nervous system development Inferred from electronic annotation. Source: UniProtKB-KW protein hexamerization Inferred from sequence or structural similarity. Source: UniProtKB protein homooligomerization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell endosome Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell spindle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alpha-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB beta-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule-severing ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 613	›613	Spastin		PRO_0000367134
Regions
	Transmembrane	54 – 74	21	Helical; Potential
	Domain	116 – 192	77	MIT
	Nucleotide binding	379 – 386	8	ATP Potential
	Region	1 – 297	297	Required for interaction with RTN1 By similarity
	Region	1 – 191	191	Required for midbody localization By similarity
	Region	1 – 77	77	Required for interaction with ATL1 By similarity
	Region	1 – 47	47	Required for nuclear localization By similarity
	Region	47 – 84	38	Required for interaction with SSNA1 and microtubules By similarity
	Region	109 – 193	85	Sufficient for interaction with CHMP1B By similarity
	Region	111 – 197	87	Required for interaction with microtubules By similarity
	Region	224 – 325	102	Sufficient for interaction with microtubules By similarity
	Region	225 – 613	389	Sufficient for microtubule severing By similarity
	Region	267 – 325	59	Required for interaction with microtubules and microtubule severing By similarity
	Motif	1 – 8	8	Nuclear localization signal By similarity
	Motif	56 – 64	9	Nuclear export signal By similarity
	Motif	306 – 309	4	Nuclear localization signal By similarity
	Compositional bias	13 – 42	30	Pro-rich
Amino acid modifications
	Modified residue	265	1	Phosphoserine By similarity
	Modified residue	300	1	Phosphothreonine By similarity
	Modified residue	303	1	Phosphothreonine By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q719N1 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: CD0E0273530C38BD	FASTA	613	67,381
	10 20 30 40 50 60 PGGRGKKKGS GGPSSPVPPR PPPPCLASSR PAPRPAPPPQ SPHKRNLYYF SYPLFLGFAL 70 80 90 100 110 120 LRLVAFHLGL LFVWLCQRFS RALMAAKRSS RAAPAPASAS PPAPVPGGEV ERVRAFHKQA 130 140 150 160 170 180 FEYISVALRI DEDEKVGQKE QAVEWYKKGI EELEKGIAVV VTGQGEQCER ARRLQAKMMT 190 200 210 220 230 240 NLVMAKDRLQ LLEKLQPVLQ FSKSQMDVYN DSTNLTCRNG HLQSESGAVP KRKDPLTHPS 250 260 270 280 290 300 NSLPRSKAIM KTGSTGLSGH HRAPSCSGLS IVSGMRQGPG PTTATHKSTP KTNRTNKPST 310 320 330 340 350 360 PTTAPRKKKD LKNFRNVDSN LANFIMNEIV DNGTAVKFDD IAGQELAKQA LQEIVILPSL 370 380 390 400 410 420 RPELFTGLRA PARGLLLFGP PGNGKTMLAK AVAAESNATF FNISAASLTS KYVGEGEKLV 430 440 450 460 470 480 RALFAVAREL QPSIIFIDEV DSLLRERREG EHDASRRLKT EFLIEFDGVQ SAGDDRVLVM 490 500 510 520 530 540 GATNRPQELD EAVLRRFIKR VYVSLPNEET RLLLLKNLLC KQGSPLTQKE LAQLARLTDG 550 560 570 580 590 600 YSGSDLTALA KDAALGPIRE LKPEQVKNMS ASEMRNIRLS DFTESLKKIK RSVSPQTLEA 610 YIRWNKDFGD TTV

References

[1]

"Analysis and mapping of CACNB4, CHRNA1, KCNJ3, SCN2A and SPG4, physiological candidate genes for porcine congenital progressive ataxia and spastic paresis." Genini S., Kratzsch A., Korczak B., Neuenschwander S., Brenig B., Jorg H., Burgi E., Ossent P., Stranzinger G., Vogeli P. J. Anim. Breed. Genet. 124:269-276(2007) [PubMed: 17868079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF540879 mRNA. Translation: AAQ11224.1. Different initiation.
RefSeq	NP_998914.1. NM_213749.1.
UniGene	Ssc.19685.
3D structure databases
HSSP	HSSP built from PDB template 1XWI based on UniProtKB O75351.
ProteinModelPortal	Q719N1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q719N1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	396584.
KEGG	ssc:396584.
Organism-specific databases
CTD	6683.
Phylogenomic databases
GeneTree	ENSGT00570000078874.
HOVERGEN	HBG108502.
OrthoDB	EOG4NZTTF.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR007330. MIT. IPR017179. Spastin. [Graphical view]
KO	K13254.
Pfam	PF00004. AAA. 1 hit. PF04212. MIT. 1 hit. [Graphical view]
PIRSF	PIRSF037338. Spastin. 1 hit.
SMART	SM00382. AAA. 1 hit. SM00745. MIT. 1 hit. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPAST_PIG

Accession

Primary (citable) accession number: Q719N1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	November 16, 2011
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program