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Protein

Thioredoxin domain-containing protein 3

Gene

Nme8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Probably required during the final stages of sperm tail maturation in the testis and/or epididymis, where extensive disulfide bonding of fibrous sheath (FS) proteins occurs. May be involved in the reduction of disulfide bonds within the sperm FS components. In vitro, it has neither NDP kinase nor reducing activity on disulfide bonds (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • cell redox homeostasis Source: InterPro
  • cellular response to reactive oxygen species Source: MGI
  • cilium assembly Source: MGI
  • flagellated sperm motility Source: MGI
  • multicellular organism development Source: UniProtKB-KW
  • spermatogenesis Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin domain-containing protein 3
Alternative name(s):
NME/NM23 family member 8
Spermatid-specific thioredoxin-2
Short name:
Sptrx-2
Gene namesi
Name:Nme8
Synonyms:Sptrx2, Txndc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1920662 Nme8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001201571 – 586Thioredoxin domain-containing protein 3Add BLAST586

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi39 ↔ 42Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ715T0
PRIDEiQ715T0

PTM databases

PhosphoSitePlusiQ715T0

Expressioni

Tissue specificityi

Testis-specific. Expressed mainly in round spermatids.1 Publication

Developmental stagei

First expressed after puberty.1 Publication

Gene expression databases

BgeeiENSMUSG00000041138
CleanExiMM_TXNDC3
ExpressionAtlasiQ715T0 baseline and differential
GenevisibleiQ715T0 MM

Interactioni

Subunit structurei

Monomer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000089358

Structurei

3D structure databases

ProteinModelPortaliQ715T0
SMRiQ715T0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 116ThioredoxinAdd BLAST107

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni157 – 254NDK 1Add BLAST98
Regioni312 – 452NDK 2Add BLAST141
Regioni453 – 586NDK 3Add BLAST134

Domaini

Sequence similaritiesi

In the C-terminal section; belongs to the NDK family.Curated

Keywords - Domaini

Redox-active center, Repeat

Phylogenomic databases

eggNOGiKOG0888 Eukaryota
KOG0907 Eukaryota
ENOG410Y8CJ LUCA
GeneTreeiENSGT00760000119146
HOGENOMiHOG000111057
HOVERGENiHBG061844
InParanoidiQ715T0
KOiK19868
OMAiSLCAQFA
OrthoDBiEOG091G03O2
PhylomeDBiQ715T0
TreeFamiTF106374

Family and domain databases

Gene3Di3.30.70.141, 3 hits
InterProiView protein in InterPro
IPR034907 NDK-like_dom
IPR036850 NDK-like_dom_sf
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF00334 NDK, 3 hits
PF00085 Thioredoxin, 1 hit
SMARTiView protein in SMART
SM00562 NDK, 2 hits
SUPFAMiSSF52833 SSF52833, 1 hit
SSF54919 SSF54919, 3 hits
PROSITEiView protein in PROSITE
PS00194 THIOREDOXIN_1, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q715T0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASKKREVQL QSVVNSQNLW DEMLLNKGLT VIDVYQAWCG PCKAVQSLFR
60 70 80 90 100
KLKNELNEDE ILHFVVAEAD NIVTLQPFRD KCEPVFLFSL NGKIIAKIQG
110 120 130 140 150
ANAPLINRKV ITLIDEERKI VAGEMDRPQY VEIPLVDAID EEYGEVQYES
160 170 180 190 200
AAEVYNMAII KPDAVLMRKN IEVREKIAKE GFVIEIQENL ILPEEVVREF
210 220 230 240 250
YTHIADQPDF EEFVVSMTNG LSCVLIVSQE DSEVIQEETL PQTDTEEEPG
260 270 280 290 300
VLEEPHVRFA PVMIKKKRDS LQEYMDRQHM SDYCDVEDDA VKVSKLIDIL
310 320 330 340 350
FPDFKTMKST NVQTTLALLH PDICEEEKDD VLNVIHNEGF TILMQRQIVL
360 370 380 390 400
SEEEARTVCK IHENEEYFDN LIGHMTSNHS YVLALRRENG VEYWKTLIGP
410 420 430 440 450
KTIEEAYASH PQSLCVQFAS GNFPTNQFYG SSSKAAAEKE IAHFFPPQST
460 470 480 490 500
LALIKPHVTH KERMEILKTI KEAGFELTLM KEMHLTPEHA NKIYFKITGK
510 520 530 540 550
DFYKNVLEVL SLGMSLVMVL TKWNAVAEWR RMVGPVDPEE AKLLSPESLR
560 570 580
AKYGLDILRN AVHGASNFSE ASEIISNVFT EGNPEN
Length:586
Mass (Da):66,857
Last modified:July 5, 2004 - v1
Checksum:i5679ED17A63F1991
GO
Isoform 2 (identifier: Q715T0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     464-470: MEILKTI → IHRSSRR
     471-586: Missing.

Note: No experimental confirmation available.
Show »
Length:470
Mass (Da):53,885
Checksum:i2F20FE1C47D91EE5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti279H → Y in AAH52356 (PubMed:15489334).Curated1
Sequence conflicti294S → F in AAH52356 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014330464 – 470MEILKTI → IHRSSRR in isoform 2. 1 Publication7
Alternative sequenceiVSP_014331471 – 586Missing in isoform 2. 1 PublicationAdd BLAST116

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF548543 mRNA Translation: AAQ12343.1
BC052356 mRNA Translation: AAH52356.1
CCDSiCCDS26262.2 [Q715T0-1]
CCDS49208.1 [Q715T0-2]
RefSeqiNP_001161381.1, NM_001167909.1 [Q715T0-2]
NP_853622.2, NM_181591.3 [Q715T0-1]
UniGeneiMm.279939

Genome annotation databases

EnsembliENSMUST00000039340; ENSMUSP00000047052; ENSMUSG00000041138 [Q715T0-2]
ENSMUST00000091763; ENSMUSP00000089358; ENSMUSG00000041138 [Q715T0-1]
GeneIDi73412
KEGGimmu:73412
UCSCiuc007ppi.1 mouse [Q715T0-2]
uc007ppj.1 mouse [Q715T0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTXND3_MOUSE
AccessioniPrimary (citable) accession number: Q715T0
Secondary accession number(s): Q80W74
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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