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Protein

Thioredoxin domain-containing protein 3

Gene

Nme8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Probably required during the final stages of sperm tail maturation in the testis and/or epididymis, where extensive disulfide bonding of fibrous sheath (FS) proteins occurs. May be involved in the reduction of disulfide bonds within the sperm FS components. In vitro, it has neither NDP kinase nor reducing activity on disulfide bonds (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • cell redox homeostasis Source: InterPro
  • cellular response to reactive oxygen species Source: MGI
  • cilium assembly Source: MGI
  • CTP biosynthetic process Source: InterPro
  • GTP biosynthetic process Source: InterPro
  • multicellular organism development Source: UniProtKB-KW
  • spermatogenesis Source: UniProtKB-KW
  • sperm motility Source: MGI
  • UTP biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin domain-containing protein 3
Alternative name(s):
NME/NM23 family member 8
Spermatid-specific thioredoxin-2
Short name:
Sptrx-2
Gene namesi
Name:Nme8
Synonyms:Sptrx2, Txndc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1920662. Nme8.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • outer dynein arm Source: MGI
  • sperm cytoplasmic droplet Source: MGI
  • sperm fibrous sheath Source: MGI
  • sperm principal piece Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 586586Thioredoxin domain-containing protein 3PRO_0000120157Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi39 ↔ 42Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ715T0.
PRIDEiQ715T0.

PTM databases

PhosphoSiteiQ715T0.

Expressioni

Tissue specificityi

Testis-specific. Expressed mainly in round spermatids.1 Publication

Developmental stagei

First expressed after puberty.1 Publication

Gene expression databases

BgeeiQ715T0.
CleanExiMM_TXNDC3.
GenevisibleiQ715T0. MM.

Interactioni

Subunit structurei

Monomer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000089358.

Structurei

3D structure databases

ProteinModelPortaliQ715T0.
SMRiQ715T0. Positions 29-102, 312-445, 448-582.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 116107ThioredoxinAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 25498NDK 1Add
BLAST
Regioni312 – 452141NDK 2Add
BLAST
Regioni453 – 586134NDK 3Add
BLAST

Domaini

Sequence similaritiesi

In the C-terminal section; belongs to the NDK family.Curated
Contains 1 thioredoxin domain.Curated

Keywords - Domaini

Redox-active center, Repeat

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
KOG0907. Eukaryota.
ENOG410Y8CJ. LUCA.
GeneTreeiENSGT00760000119146.
HOGENOMiHOG000111057.
HOVERGENiHBG061844.
InParanoidiQ715T0.
KOiK19868.
OMAiSLCAQFA.
OrthoDBiEOG751NF7.
PhylomeDBiQ715T0.
TreeFamiTF106374.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00334. NDK. 3 hits.
PF00085. Thioredoxin. 1 hit.
[Graphical view]
SMARTiSM00562. NDK. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00194. THIOREDOXIN_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q715T0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASKKREVQL QSVVNSQNLW DEMLLNKGLT VIDVYQAWCG PCKAVQSLFR
60 70 80 90 100
KLKNELNEDE ILHFVVAEAD NIVTLQPFRD KCEPVFLFSL NGKIIAKIQG
110 120 130 140 150
ANAPLINRKV ITLIDEERKI VAGEMDRPQY VEIPLVDAID EEYGEVQYES
160 170 180 190 200
AAEVYNMAII KPDAVLMRKN IEVREKIAKE GFVIEIQENL ILPEEVVREF
210 220 230 240 250
YTHIADQPDF EEFVVSMTNG LSCVLIVSQE DSEVIQEETL PQTDTEEEPG
260 270 280 290 300
VLEEPHVRFA PVMIKKKRDS LQEYMDRQHM SDYCDVEDDA VKVSKLIDIL
310 320 330 340 350
FPDFKTMKST NVQTTLALLH PDICEEEKDD VLNVIHNEGF TILMQRQIVL
360 370 380 390 400
SEEEARTVCK IHENEEYFDN LIGHMTSNHS YVLALRRENG VEYWKTLIGP
410 420 430 440 450
KTIEEAYASH PQSLCVQFAS GNFPTNQFYG SSSKAAAEKE IAHFFPPQST
460 470 480 490 500
LALIKPHVTH KERMEILKTI KEAGFELTLM KEMHLTPEHA NKIYFKITGK
510 520 530 540 550
DFYKNVLEVL SLGMSLVMVL TKWNAVAEWR RMVGPVDPEE AKLLSPESLR
560 570 580
AKYGLDILRN AVHGASNFSE ASEIISNVFT EGNPEN
Length:586
Mass (Da):66,857
Last modified:July 5, 2004 - v1
Checksum:i5679ED17A63F1991
GO
Isoform 2 (identifier: Q715T0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     464-470: MEILKTI → IHRSSRR
     471-586: Missing.

Note: No experimental confirmation available.
Show »
Length:470
Mass (Da):53,885
Checksum:i2F20FE1C47D91EE5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti279 – 2791H → Y in AAH52356 (PubMed:15489334).Curated
Sequence conflicti294 – 2941S → F in AAH52356 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei464 – 4707MEILKTI → IHRSSRR in isoform 2. 1 PublicationVSP_014330
Alternative sequencei471 – 586116Missing in isoform 2. 1 PublicationVSP_014331Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF548543 mRNA. Translation: AAQ12343.1.
BC052356 mRNA. Translation: AAH52356.1.
CCDSiCCDS26262.2. [Q715T0-1]
CCDS49208.1. [Q715T0-2]
RefSeqiNP_001161381.1. NM_001167909.1. [Q715T0-2]
NP_853622.2. NM_181591.3. [Q715T0-1]
UniGeneiMm.279939.

Genome annotation databases

EnsembliENSMUST00000039340; ENSMUSP00000047052; ENSMUSG00000041138. [Q715T0-2]
ENSMUST00000091763; ENSMUSP00000089358; ENSMUSG00000041138. [Q715T0-1]
GeneIDi73412.
KEGGimmu:73412.
UCSCiuc007ppi.1. mouse. [Q715T0-2]
uc007ppj.1. mouse. [Q715T0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF548543 mRNA. Translation: AAQ12343.1.
BC052356 mRNA. Translation: AAH52356.1.
CCDSiCCDS26262.2. [Q715T0-1]
CCDS49208.1. [Q715T0-2]
RefSeqiNP_001161381.1. NM_001167909.1. [Q715T0-2]
NP_853622.2. NM_181591.3. [Q715T0-1]
UniGeneiMm.279939.

3D structure databases

ProteinModelPortaliQ715T0.
SMRiQ715T0. Positions 29-102, 312-445, 448-582.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000089358.

PTM databases

PhosphoSiteiQ715T0.

Proteomic databases

PaxDbiQ715T0.
PRIDEiQ715T0.

Protocols and materials databases

DNASUi73412.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039340; ENSMUSP00000047052; ENSMUSG00000041138. [Q715T0-2]
ENSMUST00000091763; ENSMUSP00000089358; ENSMUSG00000041138. [Q715T0-1]
GeneIDi73412.
KEGGimmu:73412.
UCSCiuc007ppi.1. mouse. [Q715T0-2]
uc007ppj.1. mouse. [Q715T0-1]

Organism-specific databases

CTDi51314.
MGIiMGI:1920662. Nme8.

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
KOG0907. Eukaryota.
ENOG410Y8CJ. LUCA.
GeneTreeiENSGT00760000119146.
HOGENOMiHOG000111057.
HOVERGENiHBG061844.
InParanoidiQ715T0.
KOiK19868.
OMAiSLCAQFA.
OrthoDBiEOG751NF7.
PhylomeDBiQ715T0.
TreeFamiTF106374.

Miscellaneous databases

NextBioi338200.
PROiQ715T0.
SOURCEiSearch...

Gene expression databases

BgeeiQ715T0.
CleanExiMM_TXNDC3.
GenevisibleiQ715T0. MM.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00334. NDK. 3 hits.
PF00085. Thioredoxin. 1 hit.
[Graphical view]
SMARTiSM00562. NDK. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00194. THIOREDOXIN_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and developmental analysis of murid spermatid-specific thioredoxin-2 (SPTRX-2), a novel sperm fibrous sheath protein and autoantigen."
    Miranda-Vizuete A., Tsang K., Yu Y., Jimenez A., Pelto-Huikko M., Flickinger C.J., Sutovsky P., Oko R.
    J. Biol. Chem. 278:44874-44885(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: 129/SvJ.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  3. "Absolute mRNA levels and transcriptional regulation of the mouse testis-specific thioredoxins."
    Jimenez A., Prieto-Alamo M.J., Fuentes-Almagro C.A., Jurado J., Gustafsson J.-A., Pueyo C., Miranda-Vizuete A.
    Biochem. Biophys. Res. Commun. 330:65-74(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiTXND3_MOUSE
AccessioniPrimary (citable) accession number: Q715T0
Secondary accession number(s): Q80W74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.