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Protein

Thioredoxin domain-containing protein 3

Gene

Nme8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Probably required during the final stages of sperm tail maturation in the testis and/or epididymis, where extensive disulfide bonding of fibrous sheath (FS) proteins occurs. May be involved in the reduction of disulfide bonds within the sperm FS components. In vitro, it has neither NDP kinase nor reducing activity on disulfide bonds (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin domain-containing protein 3
Alternative name(s):
NME/NM23 family member 8
Spermatid-specific thioredoxin-2
Short name:
Sptrx-2
Gene namesi
Name:Nme8
Synonyms:Sptrx2, Txndc3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi735069. Nme8.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • sperm fibrous sheath Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 587587Thioredoxin domain-containing protein 3PRO_0000120158Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi39 ↔ 42Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ715S9.
PRIDEiQ715S9.

Expressioni

Tissue specificityi

Testis-specific.1 Publication

Developmental stagei

Prominently detected in the cytoplasmic lobe of step 15-18 spermatids and diminishes in step 19 just before spermiation. In the spermatid tail, it increases from step 15 to 19 and is confined to the principal piece. First detected scattered throughout the cytoplasm of the axoneme in step 14-15 spermatids, but begins to be incorporated by step 16 into the FS. During steps 17-18, it increases over the ribs and columns of the assembled FS. It peaks in step 19 and remains in the FS of epididymal spermatozoa.1 Publication

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062434.

Structurei

3D structure databases

ProteinModelPortaliQ715S9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 119118ThioredoxinPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 25599NDK 1Add
BLAST
Regioni313 – 453141NDK 2Add
BLAST
Regioni454 – 587134NDK 3Add
BLAST

Domaini

Sequence similaritiesi

In the C-terminal section; belongs to the NDK family.Curated
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
KOG0907. Eukaryota.
ENOG410Y8CJ. LUCA.
GeneTreeiENSGT00760000119146.
HOGENOMiHOG000111057.
HOVERGENiHBG061844.
InParanoidiQ715S9.
PhylomeDBiQ715S9.
TreeFamiTF106374.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00334. NDK. 3 hits.
PF00085. Thioredoxin. 1 hit.
[Graphical view]
SMARTiSM00562. NDK. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q715S9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASKKREVQL QSVVNSQNLW DEMLLNKGLT VIDVYQAWCG PCKAVQALFR
60 70 80 90 100
KLKNELNEDE LLHFVVAEAD SIVTLQPFRD KCEPVFLFSL NGKIIAKIQG
110 120 130 140 150
ANAPLINRKV IALIDEEKKI AAGEMARPQY VEIPLVDSLD EEYGEVHYES
160 170 180 190 200
NVEVYNMAVI NPDAVLMRKN LEIKEKITKE GFIIEIQENM LLPEEVAREF
210 220 230 240 250
YNHMIDEPDF EEFVYSMTNR LSCVLIISQG EDTEVIEEEA LPQSDDEEEP
260 270 280 290 300
DPLEEPHVRF APMLVKKKRD SLQEYMDRQH MSDYCHVEDD AVKVSKFIDI
310 320 330 340 350
LFPDFKTMKS TNVQRTLGLL YPEVCEEEKD NVLDIIQNEG FTILMQRQVV
360 370 380 390 400
LSEEEARAVC HVHEDEDYFD NLIGYMCSNN SYILVLMREH SVERWKELIG
410 420 430 440 450
PKTVEEAYAS HPDSLCVRFA SGNFPVNQFY GSSSKAAAET EIEHFFPPQS
460 470 480 490 500
TLALIKPHVS HKERMEILKA IRDARFELTQ MKEMHLTPEH ASKVYFKITG
510 520 530 540 550
KDFYKNVLDV LSSGMSVVMI LTKWNAVGEW RRMMGPVDPE EAKLLSPNSL
560 570 580
RARYGIDVLR NAVHGASNMS EAATAISNVF TESNFEN
Length:587
Mass (Da):67,312
Last modified:July 5, 2005 - v2
Checksum:i7FC3D94DFCE19F4B
GO
Isoform 2 (identifier: Q715S9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     154-207: Missing.

Show »
Length:533
Mass (Da):60,906
Checksum:i90987C15F2B20E51
GO

Sequence cautioni

The sequence AAQ12344.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei154 – 20754Missing in isoform 2. 1 PublicationVSP_014332Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF548544 mRNA. Translation: AAQ12344.1. Different initiation.
UniGeneiRn.107066.

Genome annotation databases

EnsembliENSRNOT00000090021; ENSRNOP00000068777; ENSRNOG00000058285. [Q715S9-1]
UCSCiRGD:735069. rat. [Q715S9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF548544 mRNA. Translation: AAQ12344.1. Different initiation.
UniGeneiRn.107066.

3D structure databases

ProteinModelPortaliQ715S9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062434.

Proteomic databases

PaxDbiQ715S9.
PRIDEiQ715S9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000090021; ENSRNOP00000068777; ENSRNOG00000058285. [Q715S9-1]
UCSCiRGD:735069. rat. [Q715S9-1]

Organism-specific databases

RGDi735069. Nme8.

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
KOG0907. Eukaryota.
ENOG410Y8CJ. LUCA.
GeneTreeiENSGT00760000119146.
HOGENOMiHOG000111057.
HOVERGENiHBG061844.
InParanoidiQ715S9.
PhylomeDBiQ715S9.
TreeFamiTF106374.

Miscellaneous databases

PROiQ715S9.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00334. NDK. 3 hits.
PF00085. Thioredoxin. 1 hit.
[Graphical view]
SMARTiSM00562. NDK. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and developmental analysis of murid spermatid-specific thioredoxin-2 (SPTRX-2), a novel sperm fibrous sheath protein and autoantigen."
    Miranda-Vizuete A., Tsang K., Yu Y., Jimenez A., Pelto-Huikko M., Flickinger C.J., Sutovsky P., Oko R.
    J. Biol. Chem. 278:44874-44885(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiTXND3_RAT
AccessioniPrimary (citable) accession number: Q715S9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: June 8, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In vasectomized rats, autoantibodies against Txndc3 are present.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.