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Protein

Thioredoxin domain-containing protein 3

Gene

Nme8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Probably required during the final stages of sperm tail maturation in the testis and/or epididymis, where extensive disulfide bonding of fibrous sheath (FS) proteins occurs. May be involved in the reduction of disulfide bonds within the sperm FS components. In vitro, it has neither NDP kinase nor reducing activity on disulfide bonds (By similarity).By similarity

Miscellaneous

In vasectomized rats, autoantibodies against Txndc3 are present.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin domain-containing protein 3
Alternative name(s):
NME/NM23 family member 8
Spermatid-specific thioredoxin-2
Short name:
Sptrx-2
Gene namesi
Name:Nme8
Synonyms:Sptrx2, Txndc3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi735069. Nme8.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001201581 – 587Thioredoxin domain-containing protein 3Add BLAST587

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi39 ↔ 42Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ715S9.
PRIDEiQ715S9.

Expressioni

Tissue specificityi

Testis-specific.1 Publication

Developmental stagei

Prominently detected in the cytoplasmic lobe of step 15-18 spermatids and diminishes in step 19 just before spermiation. In the spermatid tail, it increases from step 15 to 19 and is confined to the principal piece. First detected scattered throughout the cytoplasm of the axoneme in step 14-15 spermatids, but begins to be incorporated by step 16 into the FS. During steps 17-18, it increases over the ribs and columns of the assembled FS. It peaks in step 19 and remains in the FS of epididymal spermatozoa.1 Publication

Gene expression databases

BgeeiENSRNOG00000058285.

Interactioni

Subunit structurei

Monomer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062434.

Structurei

3D structure databases

ProteinModelPortaliQ715S9.
SMRiQ715S9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 119ThioredoxinPROSITE-ProRule annotationAdd BLAST118

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni157 – 255NDK 1Add BLAST99
Regioni313 – 453NDK 2Add BLAST141
Regioni454 – 587NDK 3Add BLAST134

Domaini

Sequence similaritiesi

In the C-terminal section; belongs to the NDK family.Curated

Keywords - Domaini

Redox-active center, Repeat

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
KOG0907. Eukaryota.
ENOG410Y8CJ. LUCA.
GeneTreeiENSGT00760000119146.
HOGENOMiHOG000111057.
HOVERGENiHBG061844.
InParanoidiQ715S9.
PhylomeDBiQ715S9.
TreeFamiTF106374.

Family and domain databases

Gene3Di3.30.70.141. 3 hits.
InterProiView protein in InterPro
IPR034907. NDK-like_dom.
IPR036850. NDK-like_dom_sf.
IPR001564. Nucleoside_diP_kinase.
IPR036249. Thioredoxin-like_sf.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
PfamiView protein in Pfam
PF00334. NDK. 3 hits.
PF00085. Thioredoxin. 1 hit.
SMARTiView protein in SMART
SM00562. NDK. 2 hits.
SUPFAMiSSF52833. SSF52833. 1 hit.
SSF54919. SSF54919. 3 hits.
PROSITEiView protein in PROSITE
PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q715S9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASKKREVQL QSVVNSQNLW DEMLLNKGLT VIDVYQAWCG PCKAVQALFR
60 70 80 90 100
KLKNELNEDE LLHFVVAEAD SIVTLQPFRD KCEPVFLFSL NGKIIAKIQG
110 120 130 140 150
ANAPLINRKV IALIDEEKKI AAGEMARPQY VEIPLVDSLD EEYGEVHYES
160 170 180 190 200
NVEVYNMAVI NPDAVLMRKN LEIKEKITKE GFIIEIQENM LLPEEVAREF
210 220 230 240 250
YNHMIDEPDF EEFVYSMTNR LSCVLIISQG EDTEVIEEEA LPQSDDEEEP
260 270 280 290 300
DPLEEPHVRF APMLVKKKRD SLQEYMDRQH MSDYCHVEDD AVKVSKFIDI
310 320 330 340 350
LFPDFKTMKS TNVQRTLGLL YPEVCEEEKD NVLDIIQNEG FTILMQRQVV
360 370 380 390 400
LSEEEARAVC HVHEDEDYFD NLIGYMCSNN SYILVLMREH SVERWKELIG
410 420 430 440 450
PKTVEEAYAS HPDSLCVRFA SGNFPVNQFY GSSSKAAAET EIEHFFPPQS
460 470 480 490 500
TLALIKPHVS HKERMEILKA IRDARFELTQ MKEMHLTPEH ASKVYFKITG
510 520 530 540 550
KDFYKNVLDV LSSGMSVVMI LTKWNAVGEW RRMMGPVDPE EAKLLSPNSL
560 570 580
RARYGIDVLR NAVHGASNMS EAATAISNVF TESNFEN
Length:587
Mass (Da):67,312
Last modified:July 5, 2005 - v2
Checksum:i7FC3D94DFCE19F4B
GO
Isoform 2 (identifier: Q715S9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     154-207: Missing.

Show »
Length:533
Mass (Da):60,906
Checksum:i90987C15F2B20E51
GO

Sequence cautioni

The sequence AAQ12344 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014332154 – 207Missing in isoform 2. 1 PublicationAdd BLAST54

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF548544 mRNA. Translation: AAQ12344.1. Different initiation.
UniGeneiRn.107066.

Genome annotation databases

EnsembliENSRNOT00000090021; ENSRNOP00000068777; ENSRNOG00000058285. [Q715S9-1]
UCSCiRGD:735069. rat. [Q715S9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTXND3_RAT
AccessioniPrimary (citable) accession number: Q715S9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: November 22, 2017
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families