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Protein

Glutamine-dependent NAD(+) synthetase

Gene

Nadsyn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamine-dependent NAD(+) synthetase (Nadsyn1)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei357 – 3571PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi355 – 3628ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-196807. Nicotinate metabolism.
UniPathwayiUPA00253; UER00334.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine-dependent NAD(+) synthetase (EC:6.3.5.1)
Alternative name(s):
NAD(+) synthase [glutamine-hydrolyzing]
NAD(+) synthetase
NH3-dependent NAD(+) synthetase-like protein
Gene namesi
Name:Nadsyn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1926164. Nadsyn1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725Glutamine-dependent NAD(+) synthetasePRO_0000237579Add
BLAST

Proteomic databases

EPDiQ711T7.
MaxQBiQ711T7.
PaxDbiQ711T7.
PRIDEiQ711T7.

PTM databases

iPTMnetiQ711T7.
PhosphoSiteiQ711T7.

Expressioni

Tissue specificityi

Highly expressed in small intestine, kidney, liver and testis. Weakly expressed in skeletal muscle, spleen, lung, heart and brain.1 Publication

Gene expression databases

BgeeiQ711T7.
CleanExiMM_NADSYN1.
ExpressionAtlasiQ711T7. baseline and differential.
GenevisibleiQ711T7. MM.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033415.

Structurei

3D structure databases

ProteinModelPortaliQ711T7.
SMRiQ711T7. Positions 5-287, 339-649.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 294290CN hydrolasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni325 – 706382LigaseBy similarityAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the NAD synthetase family.Curated
Contains 1 CN hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2303. Eukaryota.
COG0171. LUCA.
COG0388. LUCA.
GeneTreeiENSGT00390000010152.
HOGENOMiHOG000160137.
HOVERGENiHBG082007.
InParanoidiQ711T7.
KOiK01950.
OMAiSCHEDLL.
OrthoDBiEOG769ZHV.
PhylomeDBiQ711T7.
TreeFamiTF351426.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
IPR014445. Gln-dep_NAD_synthase.
IPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF006630. NADS_GAT. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
TIGRFAMsiTIGR00552. nadE. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q711T7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRKVTVATC ALNQWALDFE GNFQRILKSI QIAKGKGARY RLGPELEICG
60 70 80 90 100
YGCWDHYHES DTLLHSLQVL AALLDSPVTQ DIICDVGMPI MHRNVRYNCR
110 120 130 140 150
VIFLNRKILL IRPKMALANE GNYRELRWFT PWTRSRQTEE YVLPRMLQDL
160 170 180 190 200
TKQKTVPFGD VVLATQDTCV GSEICEELWT PRSPHIDMGL DGVEIITNAS
210 220 230 240 250
GSHHVLRKAH TRVDLVTMAT SKNGGIYLLA NQKGCDGDRL YYDGCAMIAM
260 270 280 290 300
NGSIFAQGTQ FSLDDVEVLT ATLDLEDVRS YKAEISSRNL EATRVSPYPR
310 320 330 340 350
VTVDFALSVS EDLLEPVSEP MEWTYHRPEE EISLGPACWL WDFLRRSKQA
360 370 380 390 400
GFFLPLSGGV DSAASACIVY SMCCLVCDAV KSGNQQVLTD VQNLVDESSY
410 420 430 440 450
TPQDPRELCG RLLTTCYMAS ENSSQETHSR ATKLAQLIGS YHINLSIDTA
460 470 480 490 500
VKAVLGIFSL MTGKLPRFSA HGGSSRENLA LQNVQARIRM VLAYLFAQLS
510 520 530 540 550
LWSRGARGSL LVLGSANVDE SLLGYLTKYD CSSADINPIG GISKTDLRAF
560 570 580 590 600
VQFCAERFQL PVLQTILSAP ATAELEPLAD GQVSQMDEED MGMTYAELSI
610 620 630 640 650
FGRLRKVAKA GPYSMFCKLL NMWRDSYTPT QVAEKVKLFF SKYSMNRHKM
660 670 680 690 700
TTLTPAYHAE NYSPDDNRFD LRPFLYNTRW PWQFLCIDNQ VLQLERKASQ
710 720
TREEQVLEHF KEPSPIWKQL LPKDP
Length:725
Mass (Da):81,652
Last modified:July 5, 2004 - v1
Checksum:iC1A79A9AA509B3E4
GO

Sequence cautioni

The sequence CAC88023.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti186 – 1861I → V in AAH38016 (PubMed:15489334).Curated
Sequence conflicti282 – 2821K → R in AAH38016 (PubMed:15489334).Curated
Sequence conflicti321 – 3211M → I in AAH38016 (PubMed:15489334).Curated
Sequence conflicti461 – 4611M → V in AAH38016 (PubMed:15489334).Curated
Sequence conflicti710 – 7101F → L in AAH38016 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ305343 mRNA. Translation: CAC83797.1.
AJ311612 Genomic DNA. Translation: CAC88023.1. Sequence problems.
AK020264 mRNA. Translation: BAB32048.1.
AK041101 mRNA. Translation: BAC30822.1.
AK042389 mRNA. Translation: BAC31247.1.
BC038016 mRNA. Translation: AAH38016.1.
CCDSiCCDS22047.1.
RefSeqiNP_001295024.1. NM_001308095.1.
NP_084497.1. NM_030221.2.
XP_011240323.1. XM_011242021.1.
UniGeneiMm.35104.

Genome annotation databases

EnsembliENSMUST00000033415; ENSMUSP00000033415; ENSMUSG00000031090.
GeneIDi78914.
KEGGimmu:78914.
UCSCiuc009kqa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ305343 mRNA. Translation: CAC83797.1.
AJ311612 Genomic DNA. Translation: CAC88023.1. Sequence problems.
AK020264 mRNA. Translation: BAB32048.1.
AK041101 mRNA. Translation: BAC30822.1.
AK042389 mRNA. Translation: BAC31247.1.
BC038016 mRNA. Translation: AAH38016.1.
CCDSiCCDS22047.1.
RefSeqiNP_001295024.1. NM_001308095.1.
NP_084497.1. NM_030221.2.
XP_011240323.1. XM_011242021.1.
UniGeneiMm.35104.

3D structure databases

ProteinModelPortaliQ711T7.
SMRiQ711T7. Positions 5-287, 339-649.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033415.

PTM databases

iPTMnetiQ711T7.
PhosphoSiteiQ711T7.

Proteomic databases

EPDiQ711T7.
MaxQBiQ711T7.
PaxDbiQ711T7.
PRIDEiQ711T7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033415; ENSMUSP00000033415; ENSMUSG00000031090.
GeneIDi78914.
KEGGimmu:78914.
UCSCiuc009kqa.1. mouse.

Organism-specific databases

CTDi55191.
MGIiMGI:1926164. Nadsyn1.

Phylogenomic databases

eggNOGiKOG2303. Eukaryota.
COG0171. LUCA.
COG0388. LUCA.
GeneTreeiENSGT00390000010152.
HOGENOMiHOG000160137.
HOVERGENiHBG082007.
InParanoidiQ711T7.
KOiK01950.
OMAiSCHEDLL.
OrthoDBiEOG769ZHV.
PhylomeDBiQ711T7.
TreeFamiTF351426.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00334.
ReactomeiR-MMU-196807. Nicotinate metabolism.

Miscellaneous databases

ChiTaRSiNadsyn1. mouse.
NextBioi349744.
PROiQ711T7.
SOURCEiSearch...

Gene expression databases

BgeeiQ711T7.
CleanExiMM_NADSYN1.
ExpressionAtlasiQ711T7. baseline and differential.
GenevisibleiQ711T7. MM.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
IPR014445. Gln-dep_NAD_synthase.
IPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF006630. NADS_GAT. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
TIGRFAMsiTIGR00552. nadE. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Extended sequence comparison of the BWS syndrome gene cluster on human 11p15.5 and mouse distal chromosome 7."
    Engemann S., Stroedicke M., Franck O., Walter J.
    Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/Sv.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Aorta, Cecum, Thymus and Vein.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  4. "Molecular identification of human glutamine- and ammonia-dependent NAD synthetases. Carbon-nitrogen hydrolase domain confers glutamine dependency."
    Hara N., Yamada K., Terashima M., Osago H., Shimoyama M., Tsuchiya M.
    J. Biol. Chem. 278:10914-10921(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
    Tissue: Brain.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney and Liver.

Entry informationi

Entry nameiNADE_MOUSE
AccessioniPrimary (citable) accession number: Q711T7
Secondary accession number(s): Q711P6, Q8CFY6, Q9D280
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.