Q70VZ8 (DGAT2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol O-acyltransferase 2
EC=2.3.1.20

Alternative name(s):
Diglyceride acyltransferase 2

Gene names

Name:

DGAT2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation By similarity.
Catalytic activity	Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
Enzyme regulation	Inhibited by niacin By similarity.
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the diacylglycerol acyltransferase family.

Ontologies

Keywords
Biological process	Glycerol metabolism Lipid metabolism Lipid synthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW lipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	diacylglycerol O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Diacylglycerol O-acyltransferase 2		PRO_0000249044
Regions
	Topological domain	1 – 42	42	Cytoplasmic Potential
	Transmembrane	43 – 61	19	Helical; Potential
	Topological domain	62 – 65	4	Lumenal Potential
	Transmembrane	66 – 85	20	Helical; Potential
	Topological domain	86 – 361	276	Cytoplasmic Potential
Experimental info
	Sequence conflict	35	1	A → S in ABQ12972. Ref.2
	Sequence conflict	300	1	L → P in AAT02660. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q70VZ8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C84BA6DF0242675A	FASTA	361	40,896
	10 20 30 40 50 60 MKTLIAAYSG VLRGTGSSIL SALQDLFSVT WLNRAKVEKQ LQVISVLQWV LSFLVLGVAC 70 80 90 100 110 120 SVILMYTFCT DCWLIAVLYF TWLVFDWNTP KKGGRRSQWV RNWAVWRYFR DYFPIQLVKT 130 140 150 160 170 180 HNLLTSRNYI FGYHPHGIMG LGAFCNFSTE ATEVSKKFPG IRPYLATLAG NFRMPVLREY 190 200 210 220 230 240 LMSGGICPVN RDTIDYLLSK NGSGNAIIIV VGGAAESLSS MPGKNAVTLR NRKGFVKLAL 250 260 270 280 290 300 RHGADLVPTY SFGENEVYKQ VIFEEGSWGR WVQKKFQKYI GFAPCIFHGR GLFSSDTWGL 310 320 330 340 350 360 VPYSKPITTV VGEPITIPRL ERPTQQDIDL YHAMYVQALV KLFDQHKTKF GLPETEVLEV N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic organization of the DGAT2/MOGAT gene family in cattle (Bos taurus) and other mammals." Winter A., van Eckeveld M., Bininda-Emonds O.R.P., Habermann F.A., Fries R. Cytogenet. Genome Res. 102:42-47(2003) [PubMed: 14970677] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	Xu X.R., Xu S.Z., Li J.Y. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-111; 156-205 AND 231-352, NUCLEOTIDE SEQUENCE [MRNA] OF 93-361.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ519787 mRNA. Translation: CAD58592.1. AJ534368 , AJ534369, AJ534370, AJ534371, AJ534372 Genomic DNA. Translation: CAD58968.1. BT030532 mRNA. Translation: ABQ12972.1. AY589091 Genomic DNA. Translation: AAT02657.1. AY589092 Genomic DNA. Translation: AAT02658.1. AY589093 Genomic DNA. Translation: AAT02659.1. AY589094 Genomic DNA. Translation: AAT02660.1. AY675174 mRNA. Translation: AAT78344.1.
IPI	IPI00701777.
RefSeq	NP_991362.2. NM_205793.2.
UniGene	Bt.23550.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q70VZ8.
Proteomic databases
PRIDE	Q70VZ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000001536; ENSBTAP00000001536; ENSBTAG00000001154.
GeneID	404129.
KEGG	bta:404129.
Organism-specific databases
CTD	84649.
Phylogenomic databases
eggNOG	maNOG11680.
GeneTree	ENSGT00550000074276.
HOVERGEN	HBG065791.
InParanoid	Q70VZ8.
OrthoDB	EOG4R502S.
PhylomeDB	Q70VZ8.
Family and domain databases
InterPro	IPR007130. DAGAT. [Graphical view]
KO	K11160.
PANTHER	PTHR12317. DAGAT. 1 hit.
Pfam	PF03982. DAGAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DGAT2_BOVIN

Accession

Primary (citable) accession number: Q70VZ8
Secondary accession number(s): A5D9B3 , Q6B852, Q6PP93, Q6PP94, Q6PP95, Q6PP96

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program