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Protein

Diacylglycerol O-acyltransferase 2

Gene

DGAT2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides. Functions also as an acyl-CoA retinol acyltransferase (ARAT) (By similarity).By similarity

Catalytic activityi

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
Acyl-CoA + retinol = CoA + retinyl ester.

Enzyme regulationi

Inhibited by niacin.By similarity

Pathwayi: triacylglycerol biosynthesis

This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Glycerol metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00282.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol O-acyltransferase 2 (EC:2.3.1.20)
Alternative name(s):
Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.76)
Short name:
ARAT
Short name:
Retinol O-fatty-acyltransferase
Diglyceride acyltransferase 2
Gene namesi
Name:DGAT2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4242CytoplasmicSequence analysisAdd
BLAST
Transmembranei43 – 6119HelicalSequence analysisAdd
BLAST
Topological domaini62 – 654LumenalSequence analysis
Transmembranei66 – 8520HelicalSequence analysisAdd
BLAST
Topological domaini86 – 361276CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361Diacylglycerol O-acyltransferase 2PRO_0000249044Add
BLAST

Proteomic databases

PaxDbiQ70VZ8.
PRIDEiQ70VZ8.

Interactioni

Subunit structurei

Forms multimeric complexes consisting of several DGAT2 subunits.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000001536.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0831. Eukaryota.
ENOG410XTG3. LUCA.
HOVERGENiHBG065791.
InParanoidiQ70VZ8.
KOiK11160.
OrthoDBiEOG7KH9KB.
TreeFamiTF314707.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PfamiPF03982. DAGAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q70VZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLIAAYSG VLRGTGSSIL SALQDLFSVT WLNRAKVEKQ LQVISVLQWV
60 70 80 90 100
LSFLVLGVAC SVILMYTFCT DCWLIAVLYF TWLVFDWNTP KKGGRRSQWV
110 120 130 140 150
RNWAVWRYFR DYFPIQLVKT HNLLTSRNYI FGYHPHGIMG LGAFCNFSTE
160 170 180 190 200
ATEVSKKFPG IRPYLATLAG NFRMPVLREY LMSGGICPVN RDTIDYLLSK
210 220 230 240 250
NGSGNAIIIV VGGAAESLSS MPGKNAVTLR NRKGFVKLAL RHGADLVPTY
260 270 280 290 300
SFGENEVYKQ VIFEEGSWGR WVQKKFQKYI GFAPCIFHGR GLFSSDTWGL
310 320 330 340 350
VPYSKPITTV VGEPITIPRL ERPTQQDIDL YHAMYVQALV KLFDQHKTKF
360
GLPETEVLEV N
Length:361
Mass (Da):40,896
Last modified:July 5, 2004 - v1
Checksum:iC84BA6DF0242675A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 351A → S in ABQ12972 (PubMed:16305752).Curated
Sequence conflicti300 – 3001L → P in AAT02660 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ519787 mRNA. Translation: CAD58592.1.
AJ534368
, AJ534369, AJ534370, AJ534371, AJ534372 Genomic DNA. Translation: CAD58968.1.
BT030532 mRNA. Translation: ABQ12972.1.
AY589091 Genomic DNA. Translation: AAT02657.1.
AY589092 Genomic DNA. Translation: AAT02658.1.
AY589093 Genomic DNA. Translation: AAT02659.1.
AY589094 Genomic DNA. Translation: AAT02660.1.
AY675174 mRNA. Translation: AAT78344.1.
RefSeqiNP_991362.2. NM_205793.2.
UniGeneiBt.23550.

Genome annotation databases

GeneIDi404129.
KEGGibta:404129.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ519787 mRNA. Translation: CAD58592.1.
AJ534368
, AJ534369, AJ534370, AJ534371, AJ534372 Genomic DNA. Translation: CAD58968.1.
BT030532 mRNA. Translation: ABQ12972.1.
AY589091 Genomic DNA. Translation: AAT02657.1.
AY589092 Genomic DNA. Translation: AAT02658.1.
AY589093 Genomic DNA. Translation: AAT02659.1.
AY589094 Genomic DNA. Translation: AAT02660.1.
AY675174 mRNA. Translation: AAT78344.1.
RefSeqiNP_991362.2. NM_205793.2.
UniGeneiBt.23550.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000001536.

Proteomic databases

PaxDbiQ70VZ8.
PRIDEiQ70VZ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi404129.
KEGGibta:404129.

Organism-specific databases

CTDi84649.

Phylogenomic databases

eggNOGiKOG0831. Eukaryota.
ENOG410XTG3. LUCA.
HOVERGENiHBG065791.
InParanoidiQ70VZ8.
KOiK11160.
OrthoDBiEOG7KH9KB.
TreeFamiTF314707.

Enzyme and pathway databases

UniPathwayiUPA00282.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PfamiPF03982. DAGAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic organization of the DGAT2/MOGAT gene family in cattle (Bos taurus) and other mammals."
    Winter A., van Eckeveld M., Bininda-Emonds O.R.P., Habermann F.A., Fries R.
    Cytogenet. Genome Res. 102:42-47(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. Xu X.R., Xu S.Z., Li J.Y.
    Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-111; 156-205 AND 231-352, NUCLEOTIDE SEQUENCE [MRNA] OF 93-361.

Entry informationi

Entry nameiDGAT2_BOVIN
AccessioniPrimary (citable) accession number: Q70VZ8
Secondary accession number(s): A5D9B3
, Q6B852, Q6PP93, Q6PP94, Q6PP95, Q6PP96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.