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Protein

von Willebrand factor A domain-containing protein 2

Gene

Vwa2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • identical protein binding Source: MGI

GO - Biological processi

  • calcium-independent cell-matrix adhesion Source: MGI
  • protein homooligomerization Source: UniProtKB
  • regulation of insulin receptor signaling pathway Source: MGI

Names & Taxonomyi

Protein namesi
Recommended name:
von Willebrand factor A domain-containing protein 2
Alternative name(s):
A domain-containing protein similar to matrilin and collagen
Short name:
AMACO
Gene namesi
Name:Vwa2
Synonyms:Amaco
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2684334 Vwa2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_500007039724 – 791von Willebrand factor A domain-containing protein 2Add BLAST768

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi146N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi298 ↔ 309PROSITE-ProRule annotation
Disulfide bondi303 ↔ 319PROSITE-ProRule annotation
Disulfide bondi321 ↔ 331PROSITE-ProRule annotation
Disulfide bondi715 ↔ 726PROSITE-ProRule annotation
Disulfide bondi720 ↔ 735PROSITE-ProRule annotation
Disulfide bondi737 ↔ 746PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ70UZ7
PRIDEiQ70UZ7

PTM databases

PhosphoSitePlusiQ70UZ7

Expressioni

Tissue specificityi

Detected in uterus, kidney, and skin. Also detected in intestine and lung of adult mice, and in calvaria, femur, brain, heart, intestine, skeletal muscle, and lung of newborn mice.1 Publication

Developmental stagei

First detected at days 7.5-8 dpc, when it is weakly expressed around the developing mesodermal cells. At day 10.5 dpc it is detected in the heart and the condensing somites, and at day 14.5 dpc it is present in the choroid plexus, the cochlea, the terminal bronchii of the lung, the heart, the skin, and in the cartilage primordium of the developing skeleton as well as in the interdigital spaces. Strong staining is seen in the condensed mesenchyme forming the edge of the developing teeth budding into the branchial arch and coinciding with the basement membrane that underlies the stratified squamous epithelia in the oral cavity.1 Publication

Gene expression databases

BgeeiENSMUSG00000025082
CleanExiMM_VWA2
GenevisibleiQ70UZ7 MM

Interactioni

Subunit structurei

Forms monomers and multimers.1 Publication

GO - Molecular functioni

  • identical protein binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026068

Structurei

3D structure databases

ProteinModelPortaliQ70UZ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 221VWFA 1PROSITE-ProRule annotationAdd BLAST171
Domaini295 – 332EGF-like 1PROSITE-ProRule annotationAdd BLAST38
Domaini342 – 516VWFA 2PROSITE-ProRule annotationAdd BLAST175
Domaini530 – 704VWFA 3PROSITE-ProRule annotationAdd BLAST175
Domaini711 – 747EGF-like 2PROSITE-ProRule annotationAdd BLAST37

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3544 Eukaryota
ENOG410XNMM LUCA
GeneTreeiENSGT00760000119000
HOGENOMiHOG000154835
HOVERGENiHBG055835
InParanoidiQ70UZ7
OMAiFYSWKRV
OrthoDBiEOG091G02CV
PhylomeDBiQ70UZ7
TreeFamiTF318242

Family and domain databases

Gene3Di3.40.50.410, 3 hits
InterProiView protein in InterPro
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR030755 VWA2
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
PANTHERiPTHR44880 PTHR44880, 1 hit
PfamiView protein in Pfam
PF00008 EGF, 2 hits
PF00092 VWA, 3 hits
SMARTiView protein in SMART
SM00181 EGF, 2 hits
SM00179 EGF_CA, 2 hits
SM00327 VWA, 3 hits
SUPFAMiSSF53300 SSF53300, 3 hits
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 2 hits
PS50234 VWFA, 3 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q70UZ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPPLLLLPAI YMLLFFRVSP TISLQEVHVN RETMGKIAVA SKLMWCSAAV
60 70 80 90 100
DILFLLDGSH SIGKGSFERS KRFAIAACDA LDISPGRVRV GALQFGSTPH
110 120 130 140 150
LEFPLDSFST RQEVKESIKG IVFKGGRTET GLALKRLSRG FPGGRNGSVP
160 170 180 190 200
QILIIVTDGK SQGPVALPAK QLRERGIVVF AVGVRFPRWD ELLTLASEPK
210 220 230 240 250
DRHVLLAEQV EDATNGLLST LSSSALCTTA DPDCRVEPHP CERRTLETVR
260 270 280 290 300
ELAGNALCWR GSRQADTVLA LPCPFYSWKR VFQTHPANCY RTICPGPCDS
310 320 330 340 350
QPCQNGGTCI PEGVDRYHCL CPLAFGGEVN CAPKLSLECR IDVLFLLDSS
360 370 380 390 400
AGTTLGGFRR AKAFVKRFVQ AVLREDSRAR VGIASYGRNL MVAVPVGEYQ
410 420 430 440 450
HVPDLIRSLD SIPFSGGPTL TGSALLQVAE HGFGSASRTG QDRPRRVVVL
460 470 480 490 500
LTESRSQDEV SGPAAHARAR ELLLLGVGSE ILQAELVKIT GSPKHVMVHT
510 520 530 540 550
DPQDLFSQIP ELQRRLCSQP RPGCQAQSLD LVFLLDASAS VGRENFAQMQ
560 570 580 590 600
SFIRKCTLRF DVNPDVTQVG LVVYGSRVQT AFGLDTHPTR AAVLRAMSQA
610 620 630 640 650
PYLGGVGSAG TALLHIEDKV MTVQRGARPG VPKAVVMLTG GSGAEDAAVP
660 670 680 690 700
AQKLRGNGIS VLVMSVGAVL REAVRRLAGP RDSLIHVAAY TDLPYHQDML
710 720 730 740 750
IEWLCREARL PVNLCKPSPC MNEGTCVLKN GSYRCECRGG WEGPHCENRI
760 770 780 790
LRGDAPMARS FHQEPAGLQG PTPSQQAPKH LRIGKALSSA K
Length:791
Mass (Da):85,639
Last modified:July 5, 2004 - v1
Checksum:i863CEDEA32983790
GO
Isoform 2 (identifier: Q70UZ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-218: Missing.
     219-268: STLSSSALCT...WRGSRQADTV → MCCWLSKWRM...GGRWRPSGSS

Note: No experimental confirmation available.
Show »
Length:573
Mass (Da):61,773
Checksum:i3753A8EA6E27B62B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18V → A in BAE26210 (PubMed:16141072).Curated1
Sequence conflicti156V → I in BAE26210 (PubMed:16141072).Curated1
Sequence conflicti202R → Q in BAE26210 (PubMed:16141072).Curated1
Sequence conflicti225A → E in BAE26210 (PubMed:16141072).Curated1
Sequence conflicti356G → E in BAE26210 (PubMed:16141072).Curated1
Sequence conflicti482L → V in BAE26210 (PubMed:16141072).Curated1
Sequence conflicti487V → G in BAE26210 (PubMed:16141072).Curated1
Sequence conflicti511E → K in BAE26210 (PubMed:16141072).Curated1
Sequence conflicti713N → D in BAE26210 (PubMed:16141072).Curated1
Sequence conflicti730N → I in AAI16637 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0287401 – 218Missing in isoform 2. 1 PublicationAdd BLAST218
Alternative sequenceiVSP_028741219 – 268STLSS…QADTV → MCCWLSKWRMPPMASSAPSA APHSAPLLIQTAGWNLIPVS GGRWRPSGSS in isoform 2. 1 PublicationAdd BLAST50

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ536329 mRNA Translation: CAD60277.1
AK133486 mRNA Translation: BAE21683.1
AK145058 mRNA Translation: BAE26210.1
BC115868 mRNA Translation: AAI15869.1
BC116636 mRNA Translation: AAI16637.1
CCDSiCCDS29923.1 [Q70UZ7-1]
RefSeqiNP_766428.2, NM_172840.2 [Q70UZ7-1]
UniGeneiMm.129534

Genome annotation databases

EnsembliENSMUST00000026068; ENSMUSP00000026068; ENSMUSG00000025082 [Q70UZ7-1]
GeneIDi240675
KEGGimmu:240675
UCSCiuc008hzl.1 mouse [Q70UZ7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiVWA2_MOUSE
AccessioniPrimary (citable) accession number: Q70UZ7
Secondary accession number(s): Q14AY2, Q14BI0, Q3UM88
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: July 5, 2004
Last modified: April 25, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health