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Protein

von Willebrand factor A domain-containing protein 2

Gene

Vwa2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • identical protein binding Source: MGI

GO - Biological processi

  • calcium-independent cell-matrix adhesion Source: MGI
  • protein homooligomerization Source: UniProtKB
  • regulation of insulin receptor signaling pathway Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
von Willebrand factor A domain-containing protein 2
Alternative name(s):
A domain-containing protein similar to matrilin and collagen
Short name:
AMACO
Gene namesi
Name:Vwa2
Synonyms:Amaco
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2684334. Vwa2.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: MGI
  • extracellular exosome Source: MGI
  • extracellular region Source: MGI
  • extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 791768von Willebrand factor A domain-containing protein 2PRO_5000070397Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi146 – 1461N-linked (GlcNAc...)Sequence analysis
Disulfide bondi298 ↔ 309PROSITE-ProRule annotation
Disulfide bondi303 ↔ 319PROSITE-ProRule annotation
Disulfide bondi321 ↔ 331PROSITE-ProRule annotation
Disulfide bondi715 ↔ 726PROSITE-ProRule annotation
Disulfide bondi720 ↔ 735PROSITE-ProRule annotation
Disulfide bondi737 ↔ 746PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ70UZ7.
PRIDEiQ70UZ7.

PTM databases

PhosphoSiteiQ70UZ7.

Expressioni

Tissue specificityi

Detected in uterus, kidney, and skin. Also detected in intestine and lung of adult mice, and in calvaria, femur, brain, heart, intestine, skeletal muscle, and lung of newborn mice.1 Publication

Developmental stagei

First detected at days 7.5-8 dpc, when it is weakly expressed around the developing mesodermal cells. At day 10.5 dpc it is detected in the heart and the condensing somites, and at day 14.5 dpc it is present in the choroid plexus, the cochlea, the terminal bronchii of the lung, the heart, the skin, and in the cartilage primordium of the developing skeleton as well as in the interdigital spaces. Strong staining is seen in the condensed mesenchyme forming the edge of the developing teeth budding into the branchial arch and coinciding with the basement membrane that underlies the stratified squamous epithelia in the oral cavity.1 Publication

Gene expression databases

BgeeiQ70UZ7.
CleanExiMM_VWA2.
GenevisibleiQ70UZ7. MM.

Interactioni

Subunit structurei

Forms monomers and multimers.1 Publication

GO - Molecular functioni

  • identical protein binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026068.

Structurei

3D structure databases

ProteinModelPortaliQ70UZ7.
SMRiQ70UZ7. Positions 14-211, 338-717.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 221171VWFA 1PROSITE-ProRule annotationAdd
BLAST
Domaini295 – 33238EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini342 – 516175VWFA 2PROSITE-ProRule annotationAdd
BLAST
Domaini530 – 704175VWFA 3PROSITE-ProRule annotationAdd
BLAST
Domaini711 – 74737EGF-like 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 3 VWFA domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3544. Eukaryota.
ENOG410XNMM. LUCA.
GeneTreeiENSGT00760000119000.
HOGENOMiHOG000154835.
HOVERGENiHBG055835.
InParanoidiQ70UZ7.
OMAiSCTLQFD.
OrthoDBiEOG71P290.
PhylomeDBiQ70UZ7.
TreeFamiTF318242.

Family and domain databases

Gene3Di3.40.50.410. 3 hits.
InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR030755. VWA2.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR11132:SF117. PTHR11132:SF117. 1 hit.
PfamiPF00008. EGF. 2 hits.
PF00092. VWA. 3 hits.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00179. EGF_CA. 2 hits.
SM00327. VWA. 3 hits.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 3 hits.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50234. VWFA. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q70UZ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPPLLLLPAI YMLLFFRVSP TISLQEVHVN RETMGKIAVA SKLMWCSAAV
60 70 80 90 100
DILFLLDGSH SIGKGSFERS KRFAIAACDA LDISPGRVRV GALQFGSTPH
110 120 130 140 150
LEFPLDSFST RQEVKESIKG IVFKGGRTET GLALKRLSRG FPGGRNGSVP
160 170 180 190 200
QILIIVTDGK SQGPVALPAK QLRERGIVVF AVGVRFPRWD ELLTLASEPK
210 220 230 240 250
DRHVLLAEQV EDATNGLLST LSSSALCTTA DPDCRVEPHP CERRTLETVR
260 270 280 290 300
ELAGNALCWR GSRQADTVLA LPCPFYSWKR VFQTHPANCY RTICPGPCDS
310 320 330 340 350
QPCQNGGTCI PEGVDRYHCL CPLAFGGEVN CAPKLSLECR IDVLFLLDSS
360 370 380 390 400
AGTTLGGFRR AKAFVKRFVQ AVLREDSRAR VGIASYGRNL MVAVPVGEYQ
410 420 430 440 450
HVPDLIRSLD SIPFSGGPTL TGSALLQVAE HGFGSASRTG QDRPRRVVVL
460 470 480 490 500
LTESRSQDEV SGPAAHARAR ELLLLGVGSE ILQAELVKIT GSPKHVMVHT
510 520 530 540 550
DPQDLFSQIP ELQRRLCSQP RPGCQAQSLD LVFLLDASAS VGRENFAQMQ
560 570 580 590 600
SFIRKCTLRF DVNPDVTQVG LVVYGSRVQT AFGLDTHPTR AAVLRAMSQA
610 620 630 640 650
PYLGGVGSAG TALLHIEDKV MTVQRGARPG VPKAVVMLTG GSGAEDAAVP
660 670 680 690 700
AQKLRGNGIS VLVMSVGAVL REAVRRLAGP RDSLIHVAAY TDLPYHQDML
710 720 730 740 750
IEWLCREARL PVNLCKPSPC MNEGTCVLKN GSYRCECRGG WEGPHCENRI
760 770 780 790
LRGDAPMARS FHQEPAGLQG PTPSQQAPKH LRIGKALSSA K
Length:791
Mass (Da):85,639
Last modified:July 5, 2004 - v1
Checksum:i863CEDEA32983790
GO
Isoform 2 (identifier: Q70UZ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-218: Missing.
     219-268: STLSSSALCT...WRGSRQADTV → MCCWLSKWRM...GGRWRPSGSS

Note: No experimental confirmation available.
Show »
Length:573
Mass (Da):61,773
Checksum:i3753A8EA6E27B62B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181V → A in BAE26210 (PubMed:16141072).Curated
Sequence conflicti156 – 1561V → I in BAE26210 (PubMed:16141072).Curated
Sequence conflicti202 – 2021R → Q in BAE26210 (PubMed:16141072).Curated
Sequence conflicti225 – 2251A → E in BAE26210 (PubMed:16141072).Curated
Sequence conflicti356 – 3561G → E in BAE26210 (PubMed:16141072).Curated
Sequence conflicti482 – 4821L → V in BAE26210 (PubMed:16141072).Curated
Sequence conflicti487 – 4871V → G in BAE26210 (PubMed:16141072).Curated
Sequence conflicti511 – 5111E → K in BAE26210 (PubMed:16141072).Curated
Sequence conflicti713 – 7131N → D in BAE26210 (PubMed:16141072).Curated
Sequence conflicti730 – 7301N → I in AAI16637 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 218218Missing in isoform 2. 1 PublicationVSP_028740Add
BLAST
Alternative sequencei219 – 26850STLSS…QADTV → MCCWLSKWRMPPMASSAPSA APHSAPLLIQTAGWNLIPVS GGRWRPSGSS in isoform 2. 1 PublicationVSP_028741Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ536329 mRNA. Translation: CAD60277.1.
AK133486 mRNA. Translation: BAE21683.1.
AK145058 mRNA. Translation: BAE26210.1.
BC115868 mRNA. Translation: AAI15869.1.
BC116636 mRNA. Translation: AAI16637.1.
CCDSiCCDS29923.1. [Q70UZ7-1]
RefSeqiNP_766428.2. NM_172840.2. [Q70UZ7-1]
UniGeneiMm.129534.

Genome annotation databases

EnsembliENSMUST00000026068; ENSMUSP00000026068; ENSMUSG00000025082. [Q70UZ7-1]
GeneIDi240675.
KEGGimmu:240675.
UCSCiuc008hzl.1. mouse. [Q70UZ7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ536329 mRNA. Translation: CAD60277.1.
AK133486 mRNA. Translation: BAE21683.1.
AK145058 mRNA. Translation: BAE26210.1.
BC115868 mRNA. Translation: AAI15869.1.
BC116636 mRNA. Translation: AAI16637.1.
CCDSiCCDS29923.1. [Q70UZ7-1]
RefSeqiNP_766428.2. NM_172840.2. [Q70UZ7-1]
UniGeneiMm.129534.

3D structure databases

ProteinModelPortaliQ70UZ7.
SMRiQ70UZ7. Positions 14-211, 338-717.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026068.

PTM databases

PhosphoSiteiQ70UZ7.

Proteomic databases

PaxDbiQ70UZ7.
PRIDEiQ70UZ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026068; ENSMUSP00000026068; ENSMUSG00000025082. [Q70UZ7-1]
GeneIDi240675.
KEGGimmu:240675.
UCSCiuc008hzl.1. mouse. [Q70UZ7-1]

Organism-specific databases

CTDi340706.
MGIiMGI:2684334. Vwa2.

Phylogenomic databases

eggNOGiKOG3544. Eukaryota.
ENOG410XNMM. LUCA.
GeneTreeiENSGT00760000119000.
HOGENOMiHOG000154835.
HOVERGENiHBG055835.
InParanoidiQ70UZ7.
OMAiSCTLQFD.
OrthoDBiEOG71P290.
PhylomeDBiQ70UZ7.
TreeFamiTF318242.

Miscellaneous databases

NextBioi384685.
PROiQ70UZ7.
SOURCEiSearch...

Gene expression databases

BgeeiQ70UZ7.
CleanExiMM_VWA2.
GenevisibleiQ70UZ7. MM.

Family and domain databases

Gene3Di3.40.50.410. 3 hits.
InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR030755. VWA2.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR11132:SF117. PTHR11132:SF117. 1 hit.
PfamiPF00008. EGF. 2 hits.
PF00092. VWA. 3 hits.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00179. EGF_CA. 2 hits.
SM00327. VWA. 3 hits.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 3 hits.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50234. VWFA. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of AMACO, a new member of the von Willebrand factor A-like domain protein superfamily with a regulated expression in the kidney."
    Sengle G., Kobbe B., Moergelin M., Paulsson M., Wagener R.
    J. Biol. Chem. 278:50240-50249(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, SUBUNIT.
    Strain: C57BL/6J.
    Tissue: Epiphyseal cartilage.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Mammary gland, Ovary and Uterus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).

Entry informationi

Entry nameiVWA2_MOUSE
AccessioniPrimary (citable) accession number: Q70UZ7
Secondary accession number(s): Q14AY2, Q14BI0, Q3UM88
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.