Reviewed, UniProtKB/Swiss-Prot Q70SJ2 (MPG1_KLULA)

Names and origin

Protein names

Recommended name:
    Mannose-1-phosphate guanyltransferase
    EC=2.7.7.13
Alternative name(s):
    GTP-mannose-1-phosphate guanylyltransferase
    GDP-mannose pyrophosphorylase

Gene names

Name:	MPG1
Synonyms:	PSA1
Ordered Locus Names:	KLLA0E05379g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in cell wall synthesis where it is required for glycosylation. Involved in cell cycle progression through cell-size checkpoint By similarity.
Catalytic activity	GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose. Ref.1
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the transferase hexapeptide repeat family.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Mannose-1-phosphate guanyltransferase		PRO_0000238490

Sequences

	Sequence		Length	Mass (Da)
	Q70SJ2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 6EB2DF5CDCA2148C	FASTA	361	39,320
	10 20 30 40 50 60 MKGLILVGGY GTRLRPLTLT VPKPLVEFGN RPMILHQIEA LAAAGVTDIV LAVNYRPEVM 70 80 90 100 110 120 VETLKKYEDE FGVSITFSVE TEPLGTAGPL KLAESVLKKD NSPFFVLNSD VICDYPFKEL 130 140 150 160 170 180 ADFHQAHGGK GTIVATKVDE PSKYGVIVHD IATPNLIDRF VEKPVEFVGN RINAGLYILN 190 200 210 220 230 240 PEVIDLIDLK PTSIEKETFP ILVEQKSLYS FDLEGYWMDV GQPKDFLSGT VLYLNSLSKR 250 260 270 280 290 300 DPAKLAKGEN IVGNVLVDPT AKISPTAKVG PDVVIGPNVV IGDGVRITRS VALSNSHIKD 310 320 330 340 350 360 HALVKSTIIG WNSTVGKWAR LEGVTVLGDD VEVKDEIYIN GGKVLPHKSI SVNVPKEAII M

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Enhanced secretion of heterologous proteins in Kluyveromyces lactis by overexpression of the GDP-mannose pyrophosphorylase, KlPsa1p." Uccelletti D., Staneva D., Rufini S., Venkov P., Palleschi C. FEMS Yeast Res. 5:735-746(2005) [PubMed: 15851102] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY.
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ551274 Genomic DNA. Translation: CAD82901.1. CR382125 Genomic DNA. Translation: CAG99277.1.
RefSeq	XP_454190.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q70SJ2.
Genome annotation databases
GeneID	2894004.
GenomeReviews	Gene locus KLLA0E05379g in contig CR382125_GR.
KEGG	kla:KLLA0E05379g.
Phylogenomic databases
HOGENOM	Q70SJ2.
OMA	RRIQLQP.
Enzyme and pathway databases
BRENDA	2.7.7.13. 74088.
Family and domain databases
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR005835. NTP_transferase. [Graphical view]
Pfam	PF00132. Hexapep. 4 hits. PF00483. NTP_transferase. 1 hit. [Graphical view]
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPG1_KLULA

Accession

Primary (citable) accession number: Q70SJ2
Secondary accession number(s): Q6CPE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)