Reviewed, UniProtKB/Swiss-Prot Q70PY2 (PGSB1_DROME)

Names and origin

Protein names

Recommended name:
    Peptidoglycan-recognition protein SB1
    EC=3.5.1.28

Gene names

Name:	PGRP-SB1
ORF Names:	CG9681

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	N-acetylmuramyl-L-alanine amidase involved in innate immunity by degrading bacterial peptidoglycans (PGN), preferentially DAP-type PGNs. Probably plays a scavenger role by digesting biologically active PGN into biologically inactive fragments. Ref.2
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Cofactor	Zinc By similarity.
Subcellular location	Secreted Ref.6.
Tissue specificity	In larvae, it is mainly expressed in fat body. Ref.6
Induction	Strongly up-regulated by PGN from B.subtilis. Weakly or not expressed in normal conditions. Regulated by the imd/Relish pathway. Ref.6
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Ontologies

Keywords
Biological process	Immune response Innate immunity
Cellular component	Secreted
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	innate immune response Ref.6 Non-traceable author statement. Source: UniProtKB peptidoglycan catabolic process Ref.6 Non-traceable author statement. Source: UniProtKB
Cellular component	extracellular region Ref.6 Inferred from direct assay. Source: UniProtKB
Molecular function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC peptidoglycan binding Ref.6 Non-traceable author statement. Source: UniProtKB protein binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 190	166	Peptidoglycan-recognition protein SB1		PRO_0000023907
Sites
	Metal binding	55	1	Zinc By similarity
	Metal binding	90	1	Zinc By similarity
	Metal binding	164	1	Zinc By similarity
	Metal binding	172	1	Zinc By similarity
Amino acid modifications
	Glycosylation	2	1	N-linked (GlcNAc...) Potential
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	158	1	N-linked (GlcNAc...) Potential
	Disulfide bond	62 ↔ 68		By similarity
Natural variations
	Natural variant	9	1	F → L in strain: DI7, KY038 and Loua. Ref.1
	Natural variant	36	1	V → A in strain: DI7, Loua and S30. Ref.1
	Natural variant	113	1	S → A in strain: DI7, Loua, Monty5, Tahiti and ZW141. Ref.2 Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q70PY2-1 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: A2F81E4903E8173E	FASTA	190	20,998
	10 20 30 40 50 60 MNTSTAISFV AALVLCCLAL SANALQIEPR SSWGAVSARS PSRISGAVDY VIIHHSDNPN 70 80 90 100 110 120 GCSTSEQCKR MIKNIQSDHK GRRNFSDIGY NFIVAGDGKV YEGRGFGLQG SHSPNYNRKS 130 140 150 160 170 180 IGIVFIGNFE RSAPSAQMLQ NAKDLIELAK QRGYLKDNYT LFGHRQTKAT SCPGDALYNE 190 IKTWPHWRQN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The evolution of parasite recognition genes in the innate immune system: purifying selection on Drosophila melanogaster peptidoglycan recognition proteins." Jiggins F.M., Hurst G.D.D. J. Mol. Evol. 57:598-605(2003) [PubMed: 14738318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LEU-9; ALA-36 AND ALA-113. Strain: DI7, Draveil, KY024, KY038, Loua, Monty5, P.bourg, S30, Tahiti, Texas and ZW141.
[2]	"PGRP-SB1: an N-acetylmuramoyl L-alanine amidase with antibacterial activity." Mellroth P., Steiner H. Biochem. Biophys. Res. Commun. 350:994-999(2006) [PubMed: 17046713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, VARIANT ALA-113. Tissue: Embryo.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Kapadia B., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley.
[6]	"A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster." Werner T., Liu G., Kang D., Ekengren S., Steiner H., Hultmark D. Proc. Natl. Acad. Sci. U.S.A. 97:13772-13777(2000) [PubMed: 11106397] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ556563 Genomic DNA. Translation: CAD89128.1. AJ556564 Genomic DNA. Translation: CAD89129.1. AJ556565 Genomic DNA. Translation: CAD89130.1. AJ556566 Genomic DNA. Translation: CAD89131.1. AJ556567 Genomic DNA. Translation: CAD89132.1. AJ556568 Genomic DNA. Translation: CAD89133.1. AJ556569 Genomic DNA. Translation: CAD89134.1. AJ556570 Genomic DNA. Translation: CAD89135.1. AJ556571 Genomic DNA. Translation: CAD89136.1. AJ556572 Genomic DNA. Translation: CAD89137.1. AJ556573 Genomic DNA. Translation: CAD89138.1. EF011112 mRNA. Translation: ABJ98404.1. AE014296 Genomic DNA. Translation: AAF49420.1. BT023240 mRNA. Translation: AAY55656.1. BT029423 mRNA. Translation: ABK57080.1. Different initiation.
RefSeq	NP_648917.1.
3D structure databases
SMR	Q70PY2. Positions 25-189.
ModBase	Search...
Protein-protein interaction databases
STRING	Q70PY2.
Genome annotation databases
Ensembl	FBtr0075348; FBpp0075107; FBgn0043578; Drosophila melanogaster. [Genome view]
GeneID	39870.
KEGG	dme:Dmel_CG9681.
NMPDR	fig|7227.3.peg.10199.
Organism-specific databases
CTD	39870.
FlyBase	FBgn0043578. PGRP-SB1.
Phylogenomic databases
eggNOG	inNOG09574.
InParanoid	Q70PY2.
OMA	YNFIVAG.
OrthoDB	EOG9H4693.
PhylomeDB	Q70PY2.
Enzyme and pathway databases
BRENDA	3.5.1.28. 48.
Gene expression databases
Bgee	Q70PY2.
GermOnline	CG9681. Drosophila melanogaster.
Family and domain databases
InterPro	IPR002502. Amidase_2. IPR017331. Peptidoglycan_recognition. IPR015510. PGRP. IPR006619. PGRP_met/bac. [Graphical view]
Gene3D	G3DSA:3.40.80.10. Amidase_2. 1 hit.
PANTHER	PTHR11022. PGRPs. 1 hit.
Pfam	PF01510. Amidase_2. 1 hit. [Graphical view]
PIRSF	PIRSF037945. PGRPs. 1 hit.
SMART	SM00644. Ami_2. 1 hit. SM00701. PGRP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	815805.

Entry information

Entry name

PGSB1_DROME

Accession

Primary (citable) accession number: Q70PY2
Secondary accession number(s): A0FIQ1 , A0JQ53, Q4V3W6, Q70PY3, Q70PY4, Q70PY7, Q9VV97

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 10, 2005
Last modified:	February 9, 2010
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project