Reviewed,
UniProtKB/Swiss-Prot Q70PY2 (PGSB1_DROME)
Last modified
February 9, 2010.
Version 45.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Peptidoglycan-recognition protein SB1 EC=3.5.1.28 | ||||
| Gene names |
| ||||
| Organism | Drosophila melanogaster (Fruit fly) [Complete proteome] | ||||
| Taxonomic identifier | 7227 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora |
Protein attributes
| Sequence length | 190 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | N-acetylmuramyl-L-alanine amidase involved in innate immunity by degrading bacterial peptidoglycans (PGN), preferentially DAP-type PGNs. Probably plays a scavenger role by digesting biologically active PGN into biologically inactive fragments. Ref.2 |
| Catalytic activity | Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. |
| Cofactor | Zinc By similarity. |
| Subcellular location | |
| Tissue specificity | In larvae, it is mainly expressed in fat body. Ref.6 |
| Induction | Strongly up-regulated by PGN from B.subtilis. Weakly or not expressed in normal conditions. Regulated by the imd/Relish pathway. Ref.6 |
| Sequence similarities | Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Immune response Innate immunity |
| Cellular component | Secreted |
| Domain | Signal |
| Ligand | Metal-binding Zinc |
| Molecular function | Hydrolase |
| PTM | Disulfide bond Glycoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | innate immune response Ref.6 Non-traceable author statement. Source: UniProtKB peptidoglycan catabolic process Ref.6Non-traceable author statement. Source: UniProtKB |
| Cellular component | extracellular region Ref.6 Inferred from direct assay. Source: UniProtKB |
| Molecular function | N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC peptidoglycan binding Ref.6Non-traceable author statement. Source: UniProtKB protein bindingInferred from electronic annotation. Source: InterPro zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 24 | 24 | Potential | ||||||||
| Chain | 25 – 190 | 166 | Peptidoglycan-recognition protein SB1 | PRO_0000023907 | |||||||
Sites | |||||||||||
| Metal binding | 55 | 1 | Zinc By similarity | ||||||||
| Metal binding | 90 | 1 | Zinc By similarity | ||||||||
| Metal binding | 164 | 1 | Zinc By similarity | ||||||||
| Metal binding | 172 | 1 | Zinc By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 2 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 84 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 158 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 62 ↔ 68 | By similarity | |||||||||
Natural variations | |||||||||||
| Natural variant | 9 | 1 | F → L in strain: DI7, KY038 and Loua. Ref.1 | ||||||||
| Natural variant | 36 | 1 | V → A in strain: DI7, Loua and S30. Ref.1 | ||||||||
| Natural variant | 113 | 1 | S → A in strain: DI7, Loua, Monty5, Tahiti and ZW141. Ref.2 Ref.1 | ||||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "The evolution of parasite recognition genes in the innate immune system: purifying selection on Drosophila melanogaster peptidoglycan recognition proteins." Jiggins F.M., Hurst G.D.D. J. Mol. Evol. 57:598-605(2003) [PubMed: 14738318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LEU-9; ALA-36 AND ALA-113. Strain: DI7, Draveil, KY024, KY038, Loua, Monty5, P.bourg, S30, Tahiti, Texas and ZW141. |
| [2] | "PGRP-SB1: an N-acetylmuramoyl L-alanine amidase with antibacterial activity." Mellroth P., Steiner H. Biochem. Biophys. Res. Commun. 350:994-999(2006) [PubMed: 17046713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, VARIANT ALA-113. Tissue: Embryo. |
| [3] | "The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. Venter J.C.Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley. |
| [4] | "Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. Lewis S.E.Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. |
| [5] | Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Kapadia B., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. |
| [6] | "A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster." Werner T., Liu G., Kang D., Ekengren S., Steiner H., Hultmark D. Proc. Natl. Acad. Sci. U.S.A. 97:13772-13777(2000) [PubMed: 11106397] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AJ556563 Genomic DNA. Translation: CAD89128.1. AJ556564 Genomic DNA. Translation: CAD89129.1. AJ556565 Genomic DNA. Translation: CAD89130.1. AJ556566 Genomic DNA. Translation: CAD89131.1. AJ556567 Genomic DNA. Translation: CAD89132.1. AJ556568 Genomic DNA. Translation: CAD89133.1. AJ556569 Genomic DNA. Translation: CAD89134.1. AJ556570 Genomic DNA. Translation: CAD89135.1. AJ556571 Genomic DNA. Translation: CAD89136.1. AJ556572 Genomic DNA. Translation: CAD89137.1. AJ556573 Genomic DNA. Translation: CAD89138.1. EF011112 mRNA. Translation: ABJ98404.1. AE014296 Genomic DNA. Translation: AAF49420.1. BT023240 mRNA. Translation: AAY55656.1. BT029423 mRNA. Translation: ABK57080.1. Different initiation. |
| RefSeq | NP_648917.1. |
3D structure databases | |
| SMR | Q70PY2. Positions 25-189. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q70PY2. |
Genome annotation databases | |
| Ensembl | FBtr0075348; FBpp0075107; FBgn0043578; Drosophila melanogaster. [Genome view] |
| GeneID | 39870. |
| KEGG | dme:Dmel_CG9681. |
| NMPDR | fig|7227.3.peg.10199. |
Organism-specific databases | |
| CTD | 39870. |
| FlyBase | FBgn0043578. PGRP-SB1. |
Phylogenomic databases | |
| eggNOG | inNOG09574. |
| InParanoid | Q70PY2. |
| OMA | YNFIVAG. |
| OrthoDB | EOG9H4693. |
| PhylomeDB | Q70PY2. |
Enzyme and pathway databases | |
| BRENDA | 3.5.1.28. 48. |
Gene expression databases | |
| Bgee | Q70PY2. |
| GermOnline | CG9681. Drosophila melanogaster. |
Family and domain databases | |
| InterPro | IPR002502. Amidase_2. IPR017331. Peptidoglycan_recognition. IPR015510. PGRP. IPR006619. PGRP_met/bac. [Graphical view] |
| Gene3D | G3DSA:3.40.80.10. Amidase_2. 1 hit. |
| PANTHER | PTHR11022. PGRPs. 1 hit. |
| Pfam | PF01510. Amidase_2. 1 hit. [Graphical view] |
| PIRSF | PIRSF037945. PGRPs. 1 hit. |
| SMART | SM00644. Ami_2. 1 hit. SM00701. PGRP. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 815805. |
Entry information
| Entry name | PGSB1_DROME | ||||||||
| Accession | Primary (citable) accession number: Q70PY2 Secondary accession number(s): A0FIQ1 Q9VV97 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Drosophila annotation project | ||||||||
Relevant documents
| Drosophila Drosophila: entries, gene names and cross-references to FlyBase |
| SIMILARITY comments Index of protein domains and families |

Clusters with


