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Q70PY2

- PGSB1_DROME

UniProt

Q70PY2 - PGSB1_DROME

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Protein

Peptidoglycan-recognition protein SB1

Gene

PGRP-SB1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

N-acetylmuramyl-L-alanine amidase involved in innate immunity by degrading bacterial peptidoglycans (PGN), preferentially DAP-type PGNs. Probably plays a scavenger role by digesting biologically active PGN into biologically inactive fragments.1 Publication

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactori

Zn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi55 – 551ZincBy similarity
Metal bindingi90 – 901ZincBy similarity
Metal bindingi164 – 1641ZincBy similarity
Metal bindingi172 – 1721ZincBy similarity

GO - Molecular functioni

  1. N-acetylmuramoyl-L-alanine amidase activity Source: FlyBase
  2. peptidoglycan binding Source: UniProtKB
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. immune response Source: FlyBase
  2. innate immune response Source: UniProtKB
  3. peptidoglycan catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Immunity, Innate immunity

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.1.28. 1994.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan-recognition protein SB1 (EC:3.5.1.28)
Gene namesi
Name:PGRP-SB1
ORF Names:CG9681
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0043578. PGRP-SB1.

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
  2. microtubule associated complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 190166Peptidoglycan-recognition protein SB1PRO_0000023907Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi2 – 21N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi62 ↔ 68By similarity
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi158 – 1581N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ70PY2.

Expressioni

Tissue specificityi

In larvae, it is mainly expressed in fat body.1 Publication

Inductioni

Strongly up-regulated by PGN from B.subtilis. Weakly or not expressed in normal conditions. Regulated by the imd/Relish pathway.1 Publication

Gene expression databases

BgeeiQ70PY2.

Structurei

3D structure databases

ProteinModelPortaliQ70PY2.
SMRiQ70PY2. Positions 30-189.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5479.
GeneTreeiENSGT00390000016833.
InParanoidiQ70PY2.
KOiK01446.
OMAiNEIKTWP.
OrthoDBiEOG757CZ5.
PhylomeDBiQ70PY2.

Family and domain databases

Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR017331. Peptidoglycan_recognition.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view]
PANTHERiPTHR11022. PTHR11022. 1 hit.
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
PIRSFiPIRSF037945. PGRPs. 1 hit.
SMARTiSM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q70PY2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNTSTAISFV AALVLCCLAL SANALQIEPR SSWGAVSARS PSRISGAVDY
60 70 80 90 100
VIIHHSDNPN GCSTSEQCKR MIKNIQSDHK GRRNFSDIGY NFIVAGDGKV
110 120 130 140 150
YEGRGFGLQG SHSPNYNRKS IGIVFIGNFE RSAPSAQMLQ NAKDLIELAK
160 170 180 190
QRGYLKDNYT LFGHRQTKAT SCPGDALYNE IKTWPHWRQN
Length:190
Mass (Da):20,998
Last modified:May 10, 2005 - v2
Checksum:iA2F81E4903E8173E
GO

Sequence cautioni

The sequence ABK57080.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti9 – 91F → L in strain: DI7, KY038 and Loua. 1 Publication
Natural varianti36 – 361V → A in strain: DI7, Loua and S30. 1 Publication
Natural varianti113 – 1131S → A in strain: DI7, Loua, Monty5, Tahiti and ZW141. 2 Publications

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ556563 Genomic DNA. Translation: CAD89128.1.
AJ556564 Genomic DNA. Translation: CAD89129.1.
AJ556565 Genomic DNA. Translation: CAD89130.1.
AJ556566 Genomic DNA. Translation: CAD89131.1.
AJ556567 Genomic DNA. Translation: CAD89132.1.
AJ556568 Genomic DNA. Translation: CAD89133.1.
AJ556569 Genomic DNA. Translation: CAD89134.1.
AJ556570 Genomic DNA. Translation: CAD89135.1.
AJ556571 Genomic DNA. Translation: CAD89136.1.
AJ556572 Genomic DNA. Translation: CAD89137.1.
AJ556573 Genomic DNA. Translation: CAD89138.1.
EF011112 mRNA. Translation: ABJ98404.1.
AE014296 Genomic DNA. Translation: AAF49420.1.
BT023240 mRNA. Translation: AAY55656.1.
BT029423 mRNA. Translation: ABK57080.1. Different initiation.
RefSeqiNP_648917.1. NM_140660.4.
UniGeneiDm.24753.

Genome annotation databases

EnsemblMetazoaiFBtr0075348; FBpp0075107; FBgn0043578.
GeneIDi39870.
KEGGidme:Dmel_CG9681.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ556563 Genomic DNA. Translation: CAD89128.1 .
AJ556564 Genomic DNA. Translation: CAD89129.1 .
AJ556565 Genomic DNA. Translation: CAD89130.1 .
AJ556566 Genomic DNA. Translation: CAD89131.1 .
AJ556567 Genomic DNA. Translation: CAD89132.1 .
AJ556568 Genomic DNA. Translation: CAD89133.1 .
AJ556569 Genomic DNA. Translation: CAD89134.1 .
AJ556570 Genomic DNA. Translation: CAD89135.1 .
AJ556571 Genomic DNA. Translation: CAD89136.1 .
AJ556572 Genomic DNA. Translation: CAD89137.1 .
AJ556573 Genomic DNA. Translation: CAD89138.1 .
EF011112 mRNA. Translation: ABJ98404.1 .
AE014296 Genomic DNA. Translation: AAF49420.1 .
BT023240 mRNA. Translation: AAY55656.1 .
BT029423 mRNA. Translation: ABK57080.1 . Different initiation.
RefSeqi NP_648917.1. NM_140660.4.
UniGenei Dm.24753.

3D structure databases

ProteinModelPortali Q70PY2.
SMRi Q70PY2. Positions 30-189.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q70PY2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0075348 ; FBpp0075107 ; FBgn0043578 .
GeneIDi 39870.
KEGGi dme:Dmel_CG9681.

Organism-specific databases

CTDi 39870.
FlyBasei FBgn0043578. PGRP-SB1.

Phylogenomic databases

eggNOGi COG5479.
GeneTreei ENSGT00390000016833.
InParanoidi Q70PY2.
KOi K01446.
OMAi NEIKTWP.
OrthoDBi EOG757CZ5.
PhylomeDBi Q70PY2.

Enzyme and pathway databases

BRENDAi 3.5.1.28. 1994.

Miscellaneous databases

GenomeRNAii 39870.
NextBioi 815805.

Gene expression databases

Bgeei Q70PY2.

Family and domain databases

Gene3Di 3.40.80.10. 1 hit.
InterProi IPR002502. Amidase_domain.
IPR017331. Peptidoglycan_recognition.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view ]
PANTHERi PTHR11022. PTHR11022. 1 hit.
Pfami PF01510. Amidase_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF037945. PGRPs. 1 hit.
SMARTi SM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
[Graphical view ]
SUPFAMi SSF55846. SSF55846. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The evolution of parasite recognition genes in the innate immune system: purifying selection on Drosophila melanogaster peptidoglycan recognition proteins."
    Jiggins F.M., Hurst G.D.D.
    J. Mol. Evol. 57:598-605(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LEU-9; ALA-36 AND ALA-113.
    Strain: DI7, Draveil, KY024, KY038, Loua, Monty5, P.bourg, S30, Tahiti, Texas and ZW141.
  2. "PGRP-SB1: an N-acetylmuramoyl L-alanine amidase with antibacterial activity."
    Mellroth P., Steiner H.
    Biochem. Biophys. Res. Commun. 350:994-999(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, VARIANT ALA-113.
    Tissue: Embryo.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Kapadia B., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
  6. "A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster."
    Werner T., Liu G., Kang D., Ekengren S., Steiner H., Hultmark D.
    Proc. Natl. Acad. Sci. U.S.A. 97:13772-13777(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiPGSB1_DROME
AccessioniPrimary (citable) accession number: Q70PY2
Secondary accession number(s): A0FIQ1
, A0JQ53, Q4V3W6, Q70PY3, Q70PY4, Q70PY7, Q9VV97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: November 26, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3