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Q70PY2

- PGSB1_DROME

UniProt

Q70PY2 - PGSB1_DROME

Protein

Peptidoglycan-recognition protein SB1

Gene

PGRP-SB1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 2 (10 May 2005)
      Previous versions | rss
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    Functioni

    N-acetylmuramyl-L-alanine amidase involved in innate immunity by degrading bacterial peptidoglycans (PGN), preferentially DAP-type PGNs. Probably plays a scavenger role by digesting biologically active PGN into biologically inactive fragments.1 Publication

    Catalytic activityi

    Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

    Cofactori

    Zinc.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi55 – 551ZincBy similarity
    Metal bindingi90 – 901ZincBy similarity
    Metal bindingi164 – 1641ZincBy similarity
    Metal bindingi172 – 1721ZincBy similarity

    GO - Molecular functioni

    1. N-acetylmuramoyl-L-alanine amidase activity Source: FlyBase
    2. peptidoglycan binding Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. immune response Source: FlyBase
    2. innate immune response Source: UniProtKB
    3. peptidoglycan catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Immunity, Innate immunity

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BRENDAi3.5.1.28. 1994.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peptidoglycan-recognition protein SB1 (EC:3.5.1.28)
    Gene namesi
    Name:PGRP-SB1
    ORF Names:CG9681
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3L

    Organism-specific databases

    FlyBaseiFBgn0043578. PGRP-SB1.

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB
    2. microtubule associated complex Source: FlyBase

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 190166Peptidoglycan-recognition protein SB1PRO_0000023907Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi2 – 21N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi62 ↔ 68By similarity
    Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi158 – 1581N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ70PY2.

    Expressioni

    Tissue specificityi

    In larvae, it is mainly expressed in fat body.1 Publication

    Inductioni

    Strongly up-regulated by PGN from B.subtilis. Weakly or not expressed in normal conditions. Regulated by the imd/Relish pathway.1 Publication

    Gene expression databases

    BgeeiQ70PY2.

    Structurei

    3D structure databases

    ProteinModelPortaliQ70PY2.
    SMRiQ70PY2. Positions 30-189.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5479.
    GeneTreeiENSGT00390000016833.
    InParanoidiQ70PY2.
    KOiK01446.
    OMAiNEIKTWP.
    OrthoDBiEOG757CZ5.
    PhylomeDBiQ70PY2.

    Family and domain databases

    Gene3Di3.40.80.10. 1 hit.
    InterProiIPR002502. Amidase_domain.
    IPR017331. Peptidoglycan_recognition.
    IPR015510. PGRP.
    IPR006619. PGRP_domain_met/bac.
    [Graphical view]
    PANTHERiPTHR11022. PTHR11022. 1 hit.
    PfamiPF01510. Amidase_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037945. PGRPs. 1 hit.
    SMARTiSM00644. Ami_2. 1 hit.
    SM00701. PGRP. 1 hit.
    [Graphical view]
    SUPFAMiSSF55846. SSF55846. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q70PY2-1 [UniParc]FASTAAdd to Basket

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    MNTSTAISFV AALVLCCLAL SANALQIEPR SSWGAVSARS PSRISGAVDY    50
    VIIHHSDNPN GCSTSEQCKR MIKNIQSDHK GRRNFSDIGY NFIVAGDGKV 100
    YEGRGFGLQG SHSPNYNRKS IGIVFIGNFE RSAPSAQMLQ NAKDLIELAK 150
    QRGYLKDNYT LFGHRQTKAT SCPGDALYNE IKTWPHWRQN 190
    Length:190
    Mass (Da):20,998
    Last modified:May 10, 2005 - v2
    Checksum:iA2F81E4903E8173E
    GO

    Sequence cautioni

    The sequence ABK57080.1 differs from that shown. Reason: Erroneous initiation.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti9 – 91F → L in strain: DI7, KY038 and Loua. 1 Publication
    Natural varianti36 – 361V → A in strain: DI7, Loua and S30. 1 Publication
    Natural varianti113 – 1131S → A in strain: DI7, Loua, Monty5, Tahiti and ZW141. 2 Publications

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ556563 Genomic DNA. Translation: CAD89128.1.
    AJ556564 Genomic DNA. Translation: CAD89129.1.
    AJ556565 Genomic DNA. Translation: CAD89130.1.
    AJ556566 Genomic DNA. Translation: CAD89131.1.
    AJ556567 Genomic DNA. Translation: CAD89132.1.
    AJ556568 Genomic DNA. Translation: CAD89133.1.
    AJ556569 Genomic DNA. Translation: CAD89134.1.
    AJ556570 Genomic DNA. Translation: CAD89135.1.
    AJ556571 Genomic DNA. Translation: CAD89136.1.
    AJ556572 Genomic DNA. Translation: CAD89137.1.
    AJ556573 Genomic DNA. Translation: CAD89138.1.
    EF011112 mRNA. Translation: ABJ98404.1.
    AE014296 Genomic DNA. Translation: AAF49420.1.
    BT023240 mRNA. Translation: AAY55656.1.
    BT029423 mRNA. Translation: ABK57080.1. Different initiation.
    RefSeqiNP_648917.1. NM_140660.4.
    UniGeneiDm.24753.

    Genome annotation databases

    EnsemblMetazoaiFBtr0075348; FBpp0075107; FBgn0043578.
    GeneIDi39870.
    KEGGidme:Dmel_CG9681.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ556563 Genomic DNA. Translation: CAD89128.1 .
    AJ556564 Genomic DNA. Translation: CAD89129.1 .
    AJ556565 Genomic DNA. Translation: CAD89130.1 .
    AJ556566 Genomic DNA. Translation: CAD89131.1 .
    AJ556567 Genomic DNA. Translation: CAD89132.1 .
    AJ556568 Genomic DNA. Translation: CAD89133.1 .
    AJ556569 Genomic DNA. Translation: CAD89134.1 .
    AJ556570 Genomic DNA. Translation: CAD89135.1 .
    AJ556571 Genomic DNA. Translation: CAD89136.1 .
    AJ556572 Genomic DNA. Translation: CAD89137.1 .
    AJ556573 Genomic DNA. Translation: CAD89138.1 .
    EF011112 mRNA. Translation: ABJ98404.1 .
    AE014296 Genomic DNA. Translation: AAF49420.1 .
    BT023240 mRNA. Translation: AAY55656.1 .
    BT029423 mRNA. Translation: ABK57080.1 . Different initiation.
    RefSeqi NP_648917.1. NM_140660.4.
    UniGenei Dm.24753.

    3D structure databases

    ProteinModelPortali Q70PY2.
    SMRi Q70PY2. Positions 30-189.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q70PY2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0075348 ; FBpp0075107 ; FBgn0043578 .
    GeneIDi 39870.
    KEGGi dme:Dmel_CG9681.

    Organism-specific databases

    CTDi 39870.
    FlyBasei FBgn0043578. PGRP-SB1.

    Phylogenomic databases

    eggNOGi COG5479.
    GeneTreei ENSGT00390000016833.
    InParanoidi Q70PY2.
    KOi K01446.
    OMAi NEIKTWP.
    OrthoDBi EOG757CZ5.
    PhylomeDBi Q70PY2.

    Enzyme and pathway databases

    BRENDAi 3.5.1.28. 1994.

    Miscellaneous databases

    GenomeRNAii 39870.
    NextBioi 815805.

    Gene expression databases

    Bgeei Q70PY2.

    Family and domain databases

    Gene3Di 3.40.80.10. 1 hit.
    InterProi IPR002502. Amidase_domain.
    IPR017331. Peptidoglycan_recognition.
    IPR015510. PGRP.
    IPR006619. PGRP_domain_met/bac.
    [Graphical view ]
    PANTHERi PTHR11022. PTHR11022. 1 hit.
    Pfami PF01510. Amidase_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037945. PGRPs. 1 hit.
    SMARTi SM00644. Ami_2. 1 hit.
    SM00701. PGRP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55846. SSF55846. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The evolution of parasite recognition genes in the innate immune system: purifying selection on Drosophila melanogaster peptidoglycan recognition proteins."
      Jiggins F.M., Hurst G.D.D.
      J. Mol. Evol. 57:598-605(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LEU-9; ALA-36 AND ALA-113.
      Strain: DI7, Draveil, KY024, KY038, Loua, Monty5, P.bourg, S30, Tahiti, Texas and ZW141.
    2. "PGRP-SB1: an N-acetylmuramoyl L-alanine amidase with antibacterial activity."
      Mellroth P., Steiner H.
      Biochem. Biophys. Res. Commun. 350:994-999(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, VARIANT ALA-113.
      Tissue: Embryo.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    4. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    5. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Kapadia B., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
      Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
    6. "A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster."
      Werner T., Liu G., Kang D., Ekengren S., Steiner H., Hultmark D.
      Proc. Natl. Acad. Sci. U.S.A. 97:13772-13777(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.

    Entry informationi

    Entry nameiPGSB1_DROME
    AccessioniPrimary (citable) accession number: Q70PY2
    Secondary accession number(s): A0FIQ1
    , A0JQ53, Q4V3W6, Q70PY3, Q70PY4, Q70PY7, Q9VV97
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2005
    Last sequence update: May 10, 2005
    Last modified: October 1, 2014
    This is version 76 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3