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Protein

Peptidoglycan-recognition protein SB1

Gene

PGRP-SB1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

N-acetylmuramyl-L-alanine amidase involved in innate immunity by degrading bacterial peptidoglycans (PGN), preferentially DAP-type PGNs. Probably plays a scavenger role by digesting biologically active PGN into biologically inactive fragments.1 Publication

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactori

Zn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Zinc; via pros nitrogenBy similarity1
Sitei90Important for catalytic activity; essential for amidase activity and zinc hydrate coordinationBy similarity1
Metal bindingi164Zinc; via pros nitrogenBy similarity1
Metal bindingi172ZincBy similarity1

GO - Molecular functioni

  • N-acetylmuramoyl-L-alanine amidase activity Source: FlyBase
  • peptidoglycan binding Source: FlyBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • immune response Source: FlyBase
  • innate immune response Source: UniProtKB
  • peptidoglycan catabolic process Source: FlyBase

Keywordsi

Molecular functionHydrolase
Biological processImmunity, Innate immunity
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.1.28. 1994.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan-recognition protein SB1 (EC:3.5.1.28)
Gene namesi
ORF Names:CG9681
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0043578. PGRP-SB1.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000002390725 – 190Peptidoglycan-recognition protein SB1Add BLAST166

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi2N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi62 ↔ 68By similarity
Glycosylationi84N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi158N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ70PY2.
PRIDEiQ70PY2.

Expressioni

Tissue specificityi

In larvae, it is mainly expressed in fat body.1 Publication

Inductioni

Strongly up-regulated by PGN from B.subtilis. Weakly or not expressed in normal conditions. Regulated by the imd/Relish pathway.1 Publication

Gene expression databases

BgeeiFBgn0043578.
GenevisibleiQ70PY2. DM.

Interactioni

Protein-protein interaction databases

STRINGi7227.FBpp0075107.

Structurei

3D structure databases

ProteinModelPortaliQ70PY2.
SMRiQ70PY2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IYTD. Eukaryota.
ENOG4111PQD. LUCA.
InParanoidiQ70PY2.
KOiK01446.
OMAiLGHRQTK.
OrthoDBiEOG091G0O6Z.
PhylomeDBiQ70PY2.

Family and domain databases

CDDicd06583. PGRP. 1 hit.
Gene3Di3.40.80.10. 1 hit.
InterProiView protein in InterPro
IPR002502. Amidase_domain.
IPR017331. Peptidoglycan_recognition.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
PANTHERiPTHR11022. PTHR11022. 1 hit.
PfamiView protein in Pfam
PF01510. Amidase_2. 1 hit.
PIRSFiPIRSF037945. PGRPs. 1 hit.
SMARTiView protein in SMART
SM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q70PY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTSTAISFV AALVLCCLAL SANALQIEPR SSWGAVSARS PSRISGAVDY
60 70 80 90 100
VIIHHSDNPN GCSTSEQCKR MIKNIQSDHK GRRNFSDIGY NFIVAGDGKV
110 120 130 140 150
YEGRGFGLQG SHSPNYNRKS IGIVFIGNFE RSAPSAQMLQ NAKDLIELAK
160 170 180 190
QRGYLKDNYT LFGHRQTKAT SCPGDALYNE IKTWPHWRQN
Length:190
Mass (Da):20,998
Last modified:May 10, 2005 - v2
Checksum:iA2F81E4903E8173E
GO

Sequence cautioni

The sequence ABK57080 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti9F → L in strain: DI7, KY038 and Loua. 1 Publication1
Natural varianti36V → A in strain: DI7, Loua and S30. 1 Publication1
Natural varianti113S → A in strain: DI7, Loua, Monty5, Tahiti and ZW141. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ556563 Genomic DNA. Translation: CAD89128.1.
AJ556564 Genomic DNA. Translation: CAD89129.1.
AJ556565 Genomic DNA. Translation: CAD89130.1.
AJ556566 Genomic DNA. Translation: CAD89131.1.
AJ556567 Genomic DNA. Translation: CAD89132.1.
AJ556568 Genomic DNA. Translation: CAD89133.1.
AJ556569 Genomic DNA. Translation: CAD89134.1.
AJ556570 Genomic DNA. Translation: CAD89135.1.
AJ556571 Genomic DNA. Translation: CAD89136.1.
AJ556572 Genomic DNA. Translation: CAD89137.1.
AJ556573 Genomic DNA. Translation: CAD89138.1.
EF011112 mRNA. Translation: ABJ98404.1.
AE014296 Genomic DNA. Translation: AAF49420.1.
BT023240 mRNA. Translation: AAY55656.1.
BT029423 mRNA. Translation: ABK57080.1. Different initiation.
RefSeqiNP_648917.1. NM_140660.4.
UniGeneiDm.24753.

Genome annotation databases

EnsemblMetazoaiFBtr0075348; FBpp0075107; FBgn0043578.
GeneIDi39870.
KEGGidme:Dmel_CG9681.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPGSB1_DROME
AccessioniPrimary (citable) accession number: Q70PY2
Secondary accession number(s): A0FIQ1
, A0JQ53, Q4V3W6, Q70PY3, Q70PY4, Q70PY7, Q9VV97
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: July 5, 2017
This is version 97 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families