Q70PY2 (PGSB1_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptidoglycan-recognition protein SB1
EC=3.5.1.28

Gene names

Name:	PGRP-SB1
ORF Names:	CG9681

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	N-acetylmuramyl-L-alanine amidase involved in innate immunity by degrading bacterial peptidoglycans (PGN), preferentially DAP-type PGNs. Probably plays a scavenger role by digesting biologically active PGN into biologically inactive fragments. Ref.2
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Cofactor	Zinc By similarity.
Subcellular location	Secreted Ref.6.
Tissue specificity	In larvae, it is mainly expressed in fat body. Ref.6
Induction	Strongly up-regulated by PGN from B.subtilis. Weakly or not expressed in normal conditions. Regulated by the imd/Relish pathway. Ref.6
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.
Sequence caution	The sequence ABK57080.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Immunity Innate immunity
Cellular component	Secreted
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	innate immune response Non-traceable author statement Ref.6. Source: UniProtKB peptidoglycan catabolic process Non-traceable author statement Ref.6. Source: UniProtKB
Cellular_component	extracellular region Inferred from direct assay Ref.6. Source: UniProtKB microtubule associated complex Inferred from direct assay PubMed 18433294. Source: FlyBase
Molecular_function	N-acetylmuramoyl-L-alanine amidase activity Inferred from direct assay PubMed 21364998. Source: FlyBase peptidoglycan binding Non-traceable author statement Ref.6. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 190	166	Peptidoglycan-recognition protein SB1		PRO_0000023907
Sites
	Metal binding	55	1	Zinc By similarity
	Metal binding	90	1	Zinc By similarity
	Metal binding	164	1	Zinc By similarity
	Metal binding	172	1	Zinc By similarity
Amino acid modifications
	Glycosylation	2	1	N-linked (GlcNAc...) Potential
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	158	1	N-linked (GlcNAc...) Potential
	Disulfide bond	62 ↔ 68		By similarity
Natural variations
	Natural variant	9	1	F → L in strain: DI7, KY038 and Loua. Ref.1
	Natural variant	36	1	V → A in strain: DI7, Loua and S30. Ref.1
	Natural variant	113	1	S → A in strain: DI7, Loua, Monty5, Tahiti and ZW141. Ref.1 Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q70PY2 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: A2F81E4903E8173E	FASTA	190	20,998
	10 20 30 40 50 60 MNTSTAISFV AALVLCCLAL SANALQIEPR SSWGAVSARS PSRISGAVDY VIIHHSDNPN 70 80 90 100 110 120 GCSTSEQCKR MIKNIQSDHK GRRNFSDIGY NFIVAGDGKV YEGRGFGLQG SHSPNYNRKS 130 140 150 160 170 180 IGIVFIGNFE RSAPSAQMLQ NAKDLIELAK QRGYLKDNYT LFGHRQTKAT SCPGDALYNE 190 IKTWPHWRQN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The evolution of parasite recognition genes in the innate immune system: purifying selection on Drosophila melanogaster peptidoglycan recognition proteins." Jiggins F.M., Hurst G.D.D. J. Mol. Evol. 57:598-605(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LEU-9; ALA-36 AND ALA-113. Strain: DI7, Draveil, KY024, KY038, Loua, Monty5, P.bourg, S30, Tahiti, Texas and ZW141.
[2]	"PGRP-SB1: an N-acetylmuramoyl L-alanine amidase with antibacterial activity." Mellroth P., Steiner H. Biochem. Biophys. Res. Commun. 350:994-999(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, VARIANT ALA-113. Tissue: Embryo.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Kapadia B., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley.
[6]	"A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster." Werner T., Liu G., Kang D., Ekengren S., Steiner H., Hultmark D. Proc. Natl. Acad. Sci. U.S.A. 97:13772-13777(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ556563 Genomic DNA. Translation: CAD89128.1. AJ556564 Genomic DNA. Translation: CAD89129.1. AJ556565 Genomic DNA. Translation: CAD89130.1. AJ556566 Genomic DNA. Translation: CAD89131.1. AJ556567 Genomic DNA. Translation: CAD89132.1. AJ556568 Genomic DNA. Translation: CAD89133.1. AJ556569 Genomic DNA. Translation: CAD89134.1. AJ556570 Genomic DNA. Translation: CAD89135.1. AJ556571 Genomic DNA. Translation: CAD89136.1. AJ556572 Genomic DNA. Translation: CAD89137.1. AJ556573 Genomic DNA. Translation: CAD89138.1. EF011112 mRNA. Translation: ABJ98404.1. AE014296 Genomic DNA. Translation: AAF49420.1. BT023240 mRNA. Translation: AAY55656.1. BT029423 mRNA. Translation: ABK57080.1. Different initiation.
RefSeq	NP_648917.1. NM_140660.4.
UniGene	Dm.24753.
3D structure databases
ProteinModelPortal	Q70PY2.
SMR	Q70PY2. Positions 30-189.
ModBase	Search...
Proteomic databases
PaxDb	Q70PY2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0075348; FBpp0075107; FBgn0043578.
GeneID	39870.
KEGG	dme:Dmel_CG9681.
Organism-specific databases
CTD	39870.
FlyBase	FBgn0043578. PGRP-SB1.
Phylogenomic databases
eggNOG	COG5479.
GeneTree	ENSGT00390000016833.
InParanoid	Q70PY2.
KO	K01446.
OMA	NEIKTWP.
OrthoDB	EOG48933N.
PhylomeDB	Q70PY2.
Enzyme and pathway databases
BRENDA	3.5.1.28. 1994.
Gene expression databases
Bgee	Q70PY2.
GermOnline	CG9681. Drosophila melanogaster.
Family and domain databases
Gene3D	3.40.80.10. 1 hit.
InterPro	IPR002502. Amidase_domain. IPR017331. Peptidoglycan_recognition. IPR015510. PGRP. IPR006619. PGRP_domain_met/bac. [Graphical view]
PANTHER	PTHR11022. PTHR11022. 1 hit.
Pfam	PF01510. Amidase_2. 1 hit. [Graphical view]
PIRSF	PIRSF037945. PGRPs. 1 hit.
SMART	SM00644. Ami_2. 1 hit. SM00701. PGRP. 1 hit. [Graphical view]
SUPFAM	SSF55846. Amidase_2. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	39870.
NextBio	815805.

Entry information

Entry name

PGSB1_DROME

Accession

Primary (citable) accession number: Q70PY2
Secondary accession number(s): A0FIQ1 , A0JQ53, Q4V3W6, Q70PY3, Q70PY4, Q70PY7, Q9VV97

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 10, 2005
Last modified:	May 1, 2013
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project