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Reviewed, UniProtKB/Swiss-Prot Q70LM8 (LGRE_BREPA)

Last modified January 19, 2010. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Linear gramicidin dehydrogenase lgrE
    EC=1.1.-.-
Gene names
Name: lgrE
Synonyms: lgrT
OrganismBrevibacillus parabrevis
Taxonomic identifier54914 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacillalesPaenibacillaceaeBrevibacillus

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

In the final step of gramicidin biosynthesis, reduces the pentadecapeptide-aldehyde intermediate, that is released from the terminal module of the non-ribosomal peptide synthetase lgrD, to the final product ethanolamine-containing gramicidin.

Pathway

Antibiotic biosynthesis; gramicidin S biosynthesis.

Miscellaneous

The reduction is strictly NADPH-dependent.

Linear gramicidine is a pentadecapeptide antibiotic produced during sporulation.

Sequence similarities

Belongs to the thioesterase family.

Caution

Although this protein is said to have a reductase activity, it clearly belongs to the thioesterase (hydrolase) family.

Ontologies

Keywords
   Biological processAntibiotic biosynthesis
   LigandNADP
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processantibiotic biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionhydrolase activity, acting on ester bonds

Inferred from electronic annotation. Source: InterPro

oxidoreductase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 263263Linear gramicidin dehydrogenase lgrE
PRO_0000180363

Sites

Active site961 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q70LM8-1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 885DE9A45B041641

FASTA26330,103
        10         20         30         40         50         60 
MQKTHVSPSR WLLSPKMTAE AEVLLFSFHY AGGHAGIYRE WQKKLPVQIG VCPVQLPGRS 

        70         80         90        100        110        120 
NRFMEPYYTD LSVMIRELAE ALLPHLNRPF AFFGHSMGAL VSFELARYLR NQYGIKPRHM 

       130        140        150        160        170        180 
FASGRHAPHL PDPGEAIHHL PDAEFLKGLR TLNGTPKELF ENEENEEILQ MLLPMLRADF 

       190        200        210        220        230        240 
TICEQYQYQE EEPLGCGLTA IGGWQDPDIT VAHMEAWRKH TSASFQMHML QGDHFFLHSE 

       250        260 
QEQLLAIIES TLQSYLVGYR GIG 

« Hide

References

[1]"The linear pentadecapeptide gramicidin is assembled by four multimodular nonribosomal peptide synthetases that comprise 16 modules with 57 catalytic domains."
Kessler N., Schuhmann H., Morneweg S., Linne U., Marahiel M.A.
J. Biol. Chem. 279:7413-7419(2004) [PubMed: 14670971] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 8185 / IAM 1031 / IFO 3331 / NCDO 717 / NCIB 8598.
[2]"Synthesis of linear gramicidin requires the cooperation of two independent reductases."
Schracke N., Linne U., Mahlert C., Marahiel M.A.
Biochemistry 44:8507-8513(2005) [PubMed: 15938641] [Abstract]
Cited for: CHARACTERIZATION.
Strain: ATCC 8185 / IAM 1031 / IFO 3331 / NCDO 717 / NCIB 8598.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ566197 Genomic DNA. Translation: CAD92848.1.

3D structure databases

SMRQ70LM8. Positions 16-254.
ModBaseSearch...

Family and domain databases

InterProIPR012223. TEII.
IPR001031. Thioesterase.
[Graphical view]
PANTHERPTHR11487. TEII. 1 hit.
PfamPF00975. Thioesterase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLGRE_BREPA
AccessionPrimary (citable) accession number: Q70LM8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: July 5, 2004
Last modified: January 19, 2010
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents