Reviewed, UniProtKB/Swiss-Prot Q70LM8 (LGRE_BREPA)

Names and origin

Protein names

Recommended name:
    Linear gramicidin dehydrogenase lgrE
    EC=1.1.-.-

Gene names

Name:	lgrE
Synonyms:	lgrT

Organism

Brevibacillus parabrevis

Taxonomic identifier

54914 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Paenibacillaceae › Brevibacillus

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	In the final step of gramicidin biosynthesis, reduces the pentadecapeptide-aldehyde intermediate, that is released from the terminal module of the non-ribosomal peptide synthetase lgrD, to the final product ethanolamine-containing gramicidin.
Pathway	Antibiotic biosynthesis; gramicidin S biosynthesis.
Miscellaneous	The reduction is strictly NADPH-dependent. Linear gramicidine is a pentadecapeptide antibiotic produced during sporulation.
Sequence similarities	Belongs to the thioesterase family.
Caution	Although this protein is said to have a reductase activity, it clearly belongs to the thioesterase (hydrolase) family.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Linear gramicidin dehydrogenase lgrE		PRO_0000180363
Sites
	Active site	96	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q70LM8-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 885DE9A45B041641	FASTA	263	30,103
	10 20 30 40 50 60 MQKTHVSPSR WLLSPKMTAE AEVLLFSFHY AGGHAGIYRE WQKKLPVQIG VCPVQLPGRS 70 80 90 100 110 120 NRFMEPYYTD LSVMIRELAE ALLPHLNRPF AFFGHSMGAL VSFELARYLR NQYGIKPRHM 130 140 150 160 170 180 FASGRHAPHL PDPGEAIHHL PDAEFLKGLR TLNGTPKELF ENEENEEILQ MLLPMLRADF 190 200 210 220 230 240 TICEQYQYQE EEPLGCGLTA IGGWQDPDIT VAHMEAWRKH TSASFQMHML QGDHFFLHSE 250 260 QEQLLAIIES TLQSYLVGYR GIG

References

[1]	"The linear pentadecapeptide gramicidin is assembled by four multimodular nonribosomal peptide synthetases that comprise 16 modules with 57 catalytic domains." Kessler N., Schuhmann H., Morneweg S., Linne U., Marahiel M.A. J. Biol. Chem. 279:7413-7419(2004) [PubMed: 14670971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 8185 / IAM 1031 / IFO 3331 / NCDO 717 / NCIB 8598.
[2]	"Synthesis of linear gramicidin requires the cooperation of two independent reductases." Schracke N., Linne U., Mahlert C., Marahiel M.A. Biochemistry 44:8507-8513(2005) [PubMed: 15938641] [Abstract] Cited for: CHARACTERIZATION. Strain: ATCC 8185 / IAM 1031 / IFO 3331 / NCDO 717 / NCIB 8598.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ566197 Genomic DNA. Translation: CAD92848.1.
3D structure databases
SMR	Q70LM8. Positions 16-254.
ModBase	Search...
Family and domain databases
InterPro	IPR012223. TEII. IPR001031. Thioesterase. [Graphical view]
PANTHER	PTHR11487. TEII. 1 hit.
Pfam	PF00975. Thioesterase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LGRE_BREPA

Accession

Primary (citable) accession number: Q70LM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)