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Q70KP1

- HEMA_PTV10

UniProt

Q70KP1 - HEMA_PTV10

Protein

Hemagglutinin-esterase

Gene

HE

Organism
Porcine torovirus (strain P10) (PoTV)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

    Catalytic activityi

    N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei49 – 491NucleophileBy similarity
    Active sitei336 – 3361Charge relay systemBy similarity
    Active sitei339 – 3391Charge relay systemBy similarity

    GO - Molecular functioni

    1. hydrolase activity, acting on ester bonds Source: CACAO
    2. sialate O-acetylesterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fusion of virus membrane with host plasma membrane Source: InterPro
    2. metabolic process Source: GOC

    Keywords - Molecular functioni

    Hemagglutinin, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemagglutinin-esterase (EC:3.1.1.53)
    Short name:
    HE protein
    Alternative name(s):
    E3 glycoprotein
    Gene namesi
    Name:HE
    OrganismiPorcine torovirus (strain P10) (PoTV)
    Taxonomic identifieri360395 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeTorovirinaeTorovirus
    Virus hostiSus scrofa (Pig) [TaxID: 9823]

    Subcellular locationi

    Virion membrane Curated; Single-pass type I membrane protein Curated. Host cell membrane Curated; Single-pass type I membrane protein Curated
    Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.By similarity

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 430403Hemagglutinin-esterasePRO_0000045401Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi53 ↔ 69By similarity
    Glycosylationi88 – 881N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi117 – 1171N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi120 ↔ 168By similarity
    Glycosylationi159 – 1591N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi165 – 1651N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi207 ↔ 284By similarity
    Disulfide bondi215 ↔ 257By similarity
    Glycosylationi247 – 2471N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi268 – 2681N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi289 – 2891N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi315 ↔ 320By similarity
    Glycosylationi324 – 3241N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi354 – 3541N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi357 ↔ 382By similarity

    Post-translational modificationi

    N-glycosylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliQ70KP1.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini28 – 404377Virion surfaceSequence AnalysisAdd
    BLAST
    Topological domaini426 – 4305IntravirionSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei405 – 42521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni16 – 133118Esterase domain first partBy similarityAdd
    BLAST
    Regioni134 – 274141Receptor bindingBy similarityAdd
    BLAST
    Regioni275 – 390116Esterase domain second partBy similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view]
    PfamiPF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view]
    SUPFAMiSSF49818. SSF49818. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q70KP1-1 [UniParc]FASTAAdd to Basket

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    MLRMRVRPPS AIPVFLIFVL LPFVLTSKPI TPHYGPGHIT SDWCGFGDSR    50
    SDCGNQHTPK SLDIPQELCP KFSSRTGSSM FISMHWNNDS DFNAFGYSNC 100
    GVEKVFYEGV NFSPYRNYTC YQEGSFGWVS NKVGFYSKLY SMASTSRCIK 150
    LINLDPPTNF TNYRNGTCTG NGGTAKMPDN PQLVIFNSVV KVSTQFVLPS 200
    SSDGFSCTKH LVPFCYIDGG CFEMSGVCYP FGYYYQSPSF YHAFYTNGTA 250
    GLHRYICDYL EMKPGVYNAT TFGKFLIYPT KSYCMDTMNY TVPVQAVQSI 300
    WSENRQSDDA IGQACKSPYC IFYNKTKPYL APNGADENHG DEEVRQMMQG 350
    LLVNSSCVSP QGSTPLALYS SEMIYTPNYG SCPQYYKLFE TSSDENVDVT 400
    SSAYFVATWV LLVLVIILIF ILISFCLSSY 430
    Length:430
    Mass (Da):48,225
    Last modified:July 5, 2004 - v1
    Checksum:i97A614A76EC44756
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ575366 Genomic RNA. Translation: CAE01332.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ575366 Genomic RNA. Translation: CAE01332.1 .

    3D structure databases

    ProteinModelPortali Q70KP1.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view ]
    Pfami PF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49818. SSF49818. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Phylogenetic and evolutionary relationships among torovirus field variants: evidence for multiple intertypic recombination events."
      Smits S.L., Lavazza A., Matiz K., Horzinek M.C., Koopmans M.P., de Groot R.J.
      J. Virol. 77:9567-9577(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Nidovirus sialate-O-acetylesterases: evolution and substrate specificity of coronaviral and toroviral receptor-destroying enzymes."
      Smits S.L., Gerwig G.J., van Vliet A.L., Lissenberg A., Briza P., Kamerling J.P., Vlasak R., de Groot R.J.
      J. Biol. Chem. 280:6933-6941(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiHEMA_PTV10
    AccessioniPrimary (citable) accession number: Q70KP1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3