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Q70KP1

- HEMA_PTV10

UniProt

Q70KP1 - HEMA_PTV10

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Protein

Hemagglutinin-esterase

Gene
HE
Organism
Porcine torovirus (strain P10) (PoTV)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei49 – 491Nucleophile By similarity
Active sitei?218 – ?2181Charge relay system By similarity
Active sitei339 – 3391Charge relay system By similarity

GO - Molecular functioni

  1. hydrolase activity, acting on ester bonds Source: CACAO
  2. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
  2. metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
OrganismiPorcine torovirus (strain P10) (PoTV)
Taxonomic identifieri360395 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeTorovirinaeTorovirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host cell membrane; Single-pass type I membrane protein Reviewed prediction
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 404377Virion surface Reviewed predictionAdd
BLAST
Transmembranei405 – 42521Helical; Reviewed predictionAdd
BLAST
Topological domaini426 – 4305Intravirion Reviewed prediction

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed predictionAdd
BLAST
Chaini28 – 430403Hemagglutinin-esterasePRO_0000045401Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi53 ↔ 69 By similarity
Glycosylationi88 – 881N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi117 – 1171N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi120 ↔ 168 By similarity
Glycosylationi159 – 1591N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi165 – 1651N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi207 ↔ 284 By similarity
Disulfide bondi215 ↔ 257 By similarity
Glycosylationi247 – 2471N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi268 – 2681N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi289 – 2891N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi315 ↔ 320 By similarity
Glycosylationi324 – 3241N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi354 – 3541N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi357 ↔ 382 By similarity

Post-translational modificationi

N-glycosylated By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ70KP1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni16 – 133118Esterase domain first part By similarityAdd
BLAST
Regioni134 – 274141Receptor binding By similarityAdd
BLAST
Regioni275 – 390116Esterase domain second part By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q70KP1-1 [UniParc]FASTAAdd to Basket

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MLRMRVRPPS AIPVFLIFVL LPFVLTSKPI TPHYGPGHIT SDWCGFGDSR    50
SDCGNQHTPK SLDIPQELCP KFSSRTGSSM FISMHWNNDS DFNAFGYSNC 100
GVEKVFYEGV NFSPYRNYTC YQEGSFGWVS NKVGFYSKLY SMASTSRCIK 150
LINLDPPTNF TNYRNGTCTG NGGTAKMPDN PQLVIFNSVV KVSTQFVLPS 200
SSDGFSCTKH LVPFCYIDGG CFEMSGVCYP FGYYYQSPSF YHAFYTNGTA 250
GLHRYICDYL EMKPGVYNAT TFGKFLIYPT KSYCMDTMNY TVPVQAVQSI 300
WSENRQSDDA IGQACKSPYC IFYNKTKPYL APNGADENHG DEEVRQMMQG 350
LLVNSSCVSP QGSTPLALYS SEMIYTPNYG SCPQYYKLFE TSSDENVDVT 400
SSAYFVATWV LLVLVIILIF ILISFCLSSY 430
Length:430
Mass (Da):48,225
Last modified:July 5, 2004 - v1
Checksum:i97A614A76EC44756
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ575366 Genomic RNA. Translation: CAE01332.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ575366 Genomic RNA. Translation: CAE01332.1 .

3D structure databases

ProteinModelPortali Q70KP1.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Phylogenetic and evolutionary relationships among torovirus field variants: evidence for multiple intertypic recombination events."
    Smits S.L., Lavazza A., Matiz K., Horzinek M.C., Koopmans M.P., de Groot R.J.
    J. Virol. 77:9567-9577(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Nidovirus sialate-O-acetylesterases: evolution and substrate specificity of coronaviral and toroviral receptor-destroying enzymes."
    Smits S.L., Gerwig G.J., van Vliet A.L., Lissenberg A., Briza P., Kamerling J.P., Vlasak R., de Groot R.J.
    J. Biol. Chem. 280:6933-6941(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiHEMA_PTV10
AccessioniPrimary (citable) accession number: Q70KP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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