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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Porcine torovirus (strain P10) (PoTV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei49NucleophileBy similarity1
Active sitei336Charge relay systemBy similarity1
Active sitei339Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
OrganismiPorcine torovirus (strain P10) (PoTV)
Taxonomic identifieri360395 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeTorovirinaeTorovirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 404Virion surfaceSequence analysisAdd BLAST377
Transmembranei405 – 425HelicalSequence analysisAdd BLAST21
Topological domaini426 – 430IntravirionSequence analysis5

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000004540128 – 430Hemagglutinin-esteraseAdd BLAST403

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi53 ↔ 69By similarity
Glycosylationi88N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi117N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi120 ↔ 168By similarity
Glycosylationi159N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi165N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi207 ↔ 284By similarity
Disulfide bondi215 ↔ 257By similarity
Glycosylationi247N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi268N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi289N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi315 ↔ 320By similarity
Glycosylationi324N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi354N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi357 ↔ 382By similarity

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ70KP1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni16 – 133Esterase domain first partBy similarityAdd BLAST118
Regioni134 – 274Receptor bindingBy similarityAdd BLAST141
Regioni275 – 390Esterase domain second partBy similarityAdd BLAST116

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q70KP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRMRVRPPS AIPVFLIFVL LPFVLTSKPI TPHYGPGHIT SDWCGFGDSR
60 70 80 90 100
SDCGNQHTPK SLDIPQELCP KFSSRTGSSM FISMHWNNDS DFNAFGYSNC
110 120 130 140 150
GVEKVFYEGV NFSPYRNYTC YQEGSFGWVS NKVGFYSKLY SMASTSRCIK
160 170 180 190 200
LINLDPPTNF TNYRNGTCTG NGGTAKMPDN PQLVIFNSVV KVSTQFVLPS
210 220 230 240 250
SSDGFSCTKH LVPFCYIDGG CFEMSGVCYP FGYYYQSPSF YHAFYTNGTA
260 270 280 290 300
GLHRYICDYL EMKPGVYNAT TFGKFLIYPT KSYCMDTMNY TVPVQAVQSI
310 320 330 340 350
WSENRQSDDA IGQACKSPYC IFYNKTKPYL APNGADENHG DEEVRQMMQG
360 370 380 390 400
LLVNSSCVSP QGSTPLALYS SEMIYTPNYG SCPQYYKLFE TSSDENVDVT
410 420 430
SSAYFVATWV LLVLVIILIF ILISFCLSSY
Length:430
Mass (Da):48,225
Last modified:July 5, 2004 - v1
Checksum:i97A614A76EC44756
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ575366 Genomic RNA. Translation: CAE01332.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ575366 Genomic RNA. Translation: CAE01332.1.

3D structure databases

ProteinModelPortaliQ70KP1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiHEMA_PTV10
AccessioniPrimary (citable) accession number: Q70KP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: July 5, 2004
Last modified: September 16, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.