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Q70KP1 (HEMA_PTV10) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Hemagglutinin-esterase

Short name=HE protein
EC=3.1.1.53
Alternative name(s):
E3 glycoprotein
Gene names
Name:HE
OrganismPorcine torovirus (strain P10) (PoTV)
Taxonomic identifier360395 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeTorovirinaeTorovirus
Virus hostSus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length430 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

Catalytic activity

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Subcellular location

Virion membrane; Single-pass type I membrane protein Potential. Host cell membrane; Single-pass type I membrane protein Potential. Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.

Post-translational modification

N-glycosylated By similarity.

Sequence similarities

Belongs to the influenza type C/coronaviruses hemagglutinin-esterase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 430403Hemagglutinin-esterase
PRO_0000045401

Regions

Topological domain28 – 404377Virion surface Potential
Transmembrane405 – 42521Helical; Potential
Topological domain426 – 4305Intravirion Potential
Region16 – 133118Esterase domain first part By similarity
Region134 – 274141Receptor binding By similarity
Region275 – 390116Esterase domain second part By similarity

Sites

Active site491Nucleophile By similarity
Active site?2181Charge relay system By similarity
Active site3391Charge relay system By similarity

Amino acid modifications

Glycosylation881N-linked (GlcNAc...); by host Potential
Glycosylation1171N-linked (GlcNAc...); by host Potential
Glycosylation1591N-linked (GlcNAc...); by host Potential
Glycosylation1651N-linked (GlcNAc...); by host Potential
Glycosylation2471N-linked (GlcNAc...); by host Potential
Glycosylation2681N-linked (GlcNAc...); by host Potential
Glycosylation2891N-linked (GlcNAc...); by host Potential
Glycosylation3241N-linked (GlcNAc...); by host Potential
Glycosylation3541N-linked (GlcNAc...); by host Potential
Disulfide bond53 ↔ 69 By similarity
Disulfide bond120 ↔ 168 By similarity
Disulfide bond207 ↔ 284 By similarity
Disulfide bond215 ↔ 257 By similarity
Disulfide bond315 ↔ 320 By similarity
Disulfide bond357 ↔ 382 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q70KP1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 97A614A76EC44756

FASTA43048,225
        10         20         30         40         50         60 
MLRMRVRPPS AIPVFLIFVL LPFVLTSKPI TPHYGPGHIT SDWCGFGDSR SDCGNQHTPK 

        70         80         90        100        110        120 
SLDIPQELCP KFSSRTGSSM FISMHWNNDS DFNAFGYSNC GVEKVFYEGV NFSPYRNYTC 

       130        140        150        160        170        180 
YQEGSFGWVS NKVGFYSKLY SMASTSRCIK LINLDPPTNF TNYRNGTCTG NGGTAKMPDN 

       190        200        210        220        230        240 
PQLVIFNSVV KVSTQFVLPS SSDGFSCTKH LVPFCYIDGG CFEMSGVCYP FGYYYQSPSF 

       250        260        270        280        290        300 
YHAFYTNGTA GLHRYICDYL EMKPGVYNAT TFGKFLIYPT KSYCMDTMNY TVPVQAVQSI 

       310        320        330        340        350        360 
WSENRQSDDA IGQACKSPYC IFYNKTKPYL APNGADENHG DEEVRQMMQG LLVNSSCVSP 

       370        380        390        400        410        420 
QGSTPLALYS SEMIYTPNYG SCPQYYKLFE TSSDENVDVT SSAYFVATWV LLVLVIILIF 

       430 
ILISFCLSSY 

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References

[1]"Phylogenetic and evolutionary relationships among torovirus field variants: evidence for multiple intertypic recombination events."
Smits S.L., Lavazza A., Matiz K., Horzinek M.C., Koopmans M.P., de Groot R.J.
J. Virol. 77:9567-9577(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Nidovirus sialate-O-acetylesterases: evolution and substrate specificity of coronaviral and toroviral receptor-destroying enzymes."
Smits S.L., Gerwig G.J., van Vliet A.L., Lissenberg A., Briza P., Kamerling J.P., Vlasak R., de Groot R.J.
J. Biol. Chem. 280:6933-6941(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ575366 Genomic RNA. Translation: CAE01332.1.

3D structure databases

ProteinModelPortalQ70KP1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMSSF49818. SSF49818. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHEMA_PTV10
AccessionPrimary (citable) accession number: Q70KP1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: July 5, 2004
Last modified: February 19, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families