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Q70KP1

- HEMA_PTV10

UniProt

Q70KP1 - HEMA_PTV10

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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Porcine torovirus (strain P10) (PoTV)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei49 – 491NucleophileBy similarity
Active sitei336 – 3361Charge relay systemBy similarity
Active sitei339 – 3391Charge relay systemBy similarity

GO - Molecular functioni

  1. hydrolase activity, acting on ester bonds Source: CACAO
  2. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
  2. metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
OrganismiPorcine torovirus (strain P10) (PoTV)
Taxonomic identifieri360395 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeTorovirinaeTorovirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

Virion membrane Curated; Single-pass type I membrane protein Curated. Host cell membrane Curated; Single-pass type I membrane protein Curated
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 404377Virion surfaceSequence AnalysisAdd
BLAST
Transmembranei405 – 42521HelicalSequence AnalysisAdd
BLAST
Topological domaini426 – 4305IntravirionSequence Analysis

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 430403Hemagglutinin-esterasePRO_0000045401Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi53 ↔ 69By similarity
Glycosylationi88 – 881N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi117 – 1171N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi120 ↔ 168By similarity
Glycosylationi159 – 1591N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi165 – 1651N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi207 ↔ 284By similarity
Disulfide bondi215 ↔ 257By similarity
Glycosylationi247 – 2471N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi268 – 2681N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi289 – 2891N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi315 ↔ 320By similarity
Glycosylationi324 – 3241N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi354 – 3541N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi357 ↔ 382By similarity

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ70KP1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni16 – 133118Esterase domain first partBy similarityAdd
BLAST
Regioni134 – 274141Receptor bindingBy similarityAdd
BLAST
Regioni275 – 390116Esterase domain second partBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q70KP1-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MLRMRVRPPS AIPVFLIFVL LPFVLTSKPI TPHYGPGHIT SDWCGFGDSR
60 70 80 90 100
SDCGNQHTPK SLDIPQELCP KFSSRTGSSM FISMHWNNDS DFNAFGYSNC
110 120 130 140 150
GVEKVFYEGV NFSPYRNYTC YQEGSFGWVS NKVGFYSKLY SMASTSRCIK
160 170 180 190 200
LINLDPPTNF TNYRNGTCTG NGGTAKMPDN PQLVIFNSVV KVSTQFVLPS
210 220 230 240 250
SSDGFSCTKH LVPFCYIDGG CFEMSGVCYP FGYYYQSPSF YHAFYTNGTA
260 270 280 290 300
GLHRYICDYL EMKPGVYNAT TFGKFLIYPT KSYCMDTMNY TVPVQAVQSI
310 320 330 340 350
WSENRQSDDA IGQACKSPYC IFYNKTKPYL APNGADENHG DEEVRQMMQG
360 370 380 390 400
LLVNSSCVSP QGSTPLALYS SEMIYTPNYG SCPQYYKLFE TSSDENVDVT
410 420 430
SSAYFVATWV LLVLVIILIF ILISFCLSSY
Length:430
Mass (Da):48,225
Last modified:July 5, 2004 - v1
Checksum:i97A614A76EC44756
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ575366 Genomic RNA. Translation: CAE01332.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ575366 Genomic RNA. Translation: CAE01332.1 .

3D structure databases

ProteinModelPortali Q70KP1.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Phylogenetic and evolutionary relationships among torovirus field variants: evidence for multiple intertypic recombination events."
    Smits S.L., Lavazza A., Matiz K., Horzinek M.C., Koopmans M.P., de Groot R.J.
    J. Virol. 77:9567-9577(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Nidovirus sialate-O-acetylesterases: evolution and substrate specificity of coronaviral and toroviral receptor-destroying enzymes."
    Smits S.L., Gerwig G.J., van Vliet A.L., Lissenberg A., Briza P., Kamerling J.P., Vlasak R., de Groot R.J.
    J. Biol. Chem. 280:6933-6941(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiHEMA_PTV10
AccessioniPrimary (citable) accession number: Q70KP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3