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Q70KH2 (GLCM_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucosylceramidase

EC=3.2.1.45
Alternative name(s):
Acid beta-glucosidase
Beta-glucocerebrosidase
D-glucosyl-N-acylsphingosine glucohydrolase
Gene names
Name:GBA
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length536 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Subunit structure

Interacts with SCARB2 By similarity.

Subcellular location

Lysosome membrane; Peripheral membrane protein By similarity. Note: Targeting to lysosomes occurs through an alternative MPR-independent mechanism via SCARB2 By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 30 family.

Ontologies

Keywords
   Biological processLipid metabolism
Sphingolipid metabolism
   Cellular componentLysosome
Membrane
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

sphingolipid metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentlysosomal membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglucosylceramidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3939 By similarity
Chain40 – 536497Glucosylceramidase
PRO_5000071459

Sites

Active site2741Proton donor By similarity
Active site3791Nucleophile By similarity

Amino acid modifications

Glycosylation581N-linked (GlcNAc...) Potential
Glycosylation981N-linked (GlcNAc...) Potential
Glycosylation1851N-linked (GlcNAc...) Potential
Glycosylation3091N-linked (GlcNAc...) Potential
Glycosylation5011N-linked (GlcNAc...) Potential
Disulfide bond43 ↔ 55 By similarity
Disulfide bond57 ↔ 62 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q70KH2 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 25F24D3D548D0C9E

FASTA53659,874
        10         20         30         40         50         60 
MELSSPSREE CPRPQGRVGI MAASLMGLLL LQAASWASGA RPCSPKSFGY SSVVCVCNAT 

        70         80         90        100        110        120 
YCDSLDPLTL PDPGTFSRFE STRSGRRMEL SLGTFQANRT SKGLLLTLQP DQKFQKVKGF 

       130        140        150        160        170        180 
GGAMTDAAAL NILALSPQAR NLLLKSYFSE EGIEYNIIRV PMASCDFSIR IYTYADTPDD 

       190        200        210        220        230        240 
FQLLNFSLPE EDVKLKIPLI HQALKMAQRP VSLFASPWTS PTWLKTNGAV NGKGTLKGHP 

       250        260        270        280        290        300 
GDRYHQTWAK YFVKFLDAYA EHNLHFWAVT AENEPSAGLF TGYPFQCLGF TPEHQRDFIA 

       310        320        330        340        350        360 
RDPGPTLANS THRNVRLLML DDQRLLLPHW AQVVLADPEA AKYVHGIAVH WYLDFLAPAK 

       370        380        390        400        410        420 
ATLGETHRLF PNTMLFASEA CVGSKFWEQS VRLGSWDRGV QYSHSIITNL LYHVVGWTDW 

       430        440        450        460        470        480 
NLALNPEGGP NWVRNFVDSP IIVDISKDTF YKQPMFYHLG HFSKFIPEGS QRVGLAASEK 

       490        500        510        520        530 
NNLDTVALLR PDGSAVVVVL NRSSKDVPLT IKDPALGFLE TISPSYSIHT YLWHRK 

« Hide

References

[1]"Comparative and genetic analysis of the porcine glucocerebrosidase (GBA) gene."
Stratil A., Wagenknecht D., Van Poucke M., Kubickova S., Bartenschlager H., Musilova P., Rubes J., Geldermann H., Peelman L.J.
Comp. Biochem. Physiol. 138B:377-383(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ575649 Genomic DNA. Translation: CAE06498.1.
RefSeqNP_001005730.1. NM_001005730.1.
UniGeneSsc.45597.

3D structure databases

ProteinModelPortalQ70KH2.
SMRQ70KH2. Positions 40-536.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH30. Glycoside Hydrolase Family 30.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID449572.
KEGGssc:449572.

Organism-specific databases

CTD2629.

Phylogenomic databases

eggNOGCOG5520.
HOGENOMHOG000270031.
HOVERGENHBG002285.
KOK01201.

Enzyme and pathway databases

BRENDA3.2.1.45. 6170.

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR11069. PTHR11069. 1 hit.
PfamPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSPR00843. GLHYDRLASE30.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLCM_PIG
AccessionPrimary (citable) accession number: Q70KH2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: July 5, 2004
Last modified: November 13, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries