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Q70KH2

- GLCM_PIG

UniProt

Q70KH2 - GLCM_PIG

Protein

Glucosylceramidase

Gene

GBA

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei274 – 2741Proton donorBy similarity
    Active sitei379 – 3791NucleophileBy similarity

    GO - Molecular functioni

    1. glucosylceramidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. sphingolipid metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Enzyme and pathway databases

    BRENDAi3.2.1.45. 6170.

    Protein family/group databases

    CAZyiGH30. Glycoside Hydrolase Family 30.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucosylceramidase (EC:3.2.1.45)
    Alternative name(s):
    Acid beta-glucosidase
    Beta-glucocerebrosidase
    D-glucosyl-N-acylsphingosine glucohydrolase
    Gene namesi
    Name:GBA
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    Lysosome membrane By similarity; Peripheral membrane protein By similarity
    Note: Targeting to lysosomes occurs through an alternative MPR-independent mechanism via SCARB2.By similarity

    GO - Cellular componenti

    1. lysosomal membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Lysosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3939By similarityAdd
    BLAST
    Chaini40 – 536497GlucosylceramidasePRO_5000071459Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi43 ↔ 55By similarity
    Disulfide bondi57 ↔ 62By similarity
    Glycosylationi58 – 581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi185 – 1851N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi309 – 3091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi501 – 5011N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Interacts with SCARB2.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ70KH2.
    SMRiQ70KH2. Positions 40-536.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 30 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5520.
    HOGENOMiHOG000270031.
    HOVERGENiHBG002285.
    KOiK01201.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR013780. Glyco_hydro_13_b.
    IPR001139. Glyco_hydro_30.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR11069. PTHR11069. 1 hit.
    PfamiPF02055. Glyco_hydro_30. 1 hit.
    [Graphical view]
    PRINTSiPR00843. GLHYDRLASE30.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q70KH2-1 [UniParc]FASTAAdd to Basket

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    MELSSPSREE CPRPQGRVGI MAASLMGLLL LQAASWASGA RPCSPKSFGY    50
    SSVVCVCNAT YCDSLDPLTL PDPGTFSRFE STRSGRRMEL SLGTFQANRT 100
    SKGLLLTLQP DQKFQKVKGF GGAMTDAAAL NILALSPQAR NLLLKSYFSE 150
    EGIEYNIIRV PMASCDFSIR IYTYADTPDD FQLLNFSLPE EDVKLKIPLI 200
    HQALKMAQRP VSLFASPWTS PTWLKTNGAV NGKGTLKGHP GDRYHQTWAK 250
    YFVKFLDAYA EHNLHFWAVT AENEPSAGLF TGYPFQCLGF TPEHQRDFIA 300
    RDPGPTLANS THRNVRLLML DDQRLLLPHW AQVVLADPEA AKYVHGIAVH 350
    WYLDFLAPAK ATLGETHRLF PNTMLFASEA CVGSKFWEQS VRLGSWDRGV 400
    QYSHSIITNL LYHVVGWTDW NLALNPEGGP NWVRNFVDSP IIVDISKDTF 450
    YKQPMFYHLG HFSKFIPEGS QRVGLAASEK NNLDTVALLR PDGSAVVVVL 500
    NRSSKDVPLT IKDPALGFLE TISPSYSIHT YLWHRK 536
    Length:536
    Mass (Da):59,874
    Last modified:July 5, 2004 - v1
    Checksum:i25F24D3D548D0C9E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ575649 Genomic DNA. Translation: CAE06498.1.
    RefSeqiNP_001005730.1. NM_001005730.1.
    UniGeneiSsc.45597.

    Genome annotation databases

    GeneIDi449572.
    KEGGissc:449572.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ575649 Genomic DNA. Translation: CAE06498.1 .
    RefSeqi NP_001005730.1. NM_001005730.1.
    UniGenei Ssc.45597.

    3D structure databases

    ProteinModelPortali Q70KH2.
    SMRi Q70KH2. Positions 40-536.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH30. Glycoside Hydrolase Family 30.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 449572.
    KEGGi ssc:449572.

    Organism-specific databases

    CTDi 2629.

    Phylogenomic databases

    eggNOGi COG5520.
    HOGENOMi HOG000270031.
    HOVERGENi HBG002285.
    KOi K01201.

    Enzyme and pathway databases

    BRENDAi 3.2.1.45. 6170.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR013780. Glyco_hydro_13_b.
    IPR001139. Glyco_hydro_30.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR11069. PTHR11069. 1 hit.
    Pfami PF02055. Glyco_hydro_30. 1 hit.
    [Graphical view ]
    PRINTSi PR00843. GLHYDRLASE30.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiGLCM_PIG
    AccessioniPrimary (citable) accession number: Q70KH2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3