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Q70KH2

- GLCM_PIG

UniProt

Q70KH2 - GLCM_PIG

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Protein

Glucosylceramidase

Gene

GBA

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei274 – 2741Proton donorBy similarity
Active sitei379 – 3791NucleophileBy similarity

GO - Molecular functioni

  1. glucosylceramidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. sphingolipid metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

BRENDAi3.2.1.45. 6170.

Protein family/group databases

CAZyiGH30. Glycoside Hydrolase Family 30.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosylceramidase (EC:3.2.1.45)
Alternative name(s):
Acid beta-glucosidase
Beta-glucocerebrosidase
D-glucosyl-N-acylsphingosine glucohydrolase
Gene namesi
Name:GBA
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Lysosome membrane By similarity; Peripheral membrane protein By similarity
Note: Targeting to lysosomes occurs through an alternative MPR-independent mechanism via SCARB2.By similarity

GO - Cellular componenti

  1. lysosome Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3939By similarityAdd
BLAST
Chaini40 – 536497GlucosylceramidasePRO_5000071459Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi43 ↔ 55By similarity
Disulfide bondi57 ↔ 62By similarity
Glycosylationi58 – 581N-linked (GlcNAc...)Sequence Analysis
Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
Glycosylationi185 – 1851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi309 – 3091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi501 – 5011N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Interacts with SCARB2.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ70KH2.
SMRiQ70KH2. Positions 40-536.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 30 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5520.
HOGENOMiHOG000270031.
HOVERGENiHBG002285.
InParanoidiQ70KH2.
KOiK01201.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11069. PTHR11069. 1 hit.
PfamiPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSiPR00843. GLHYDRLASE30.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q70KH2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELSSPSREE CPRPQGRVGI MAASLMGLLL LQAASWASGA RPCSPKSFGY
60 70 80 90 100
SSVVCVCNAT YCDSLDPLTL PDPGTFSRFE STRSGRRMEL SLGTFQANRT
110 120 130 140 150
SKGLLLTLQP DQKFQKVKGF GGAMTDAAAL NILALSPQAR NLLLKSYFSE
160 170 180 190 200
EGIEYNIIRV PMASCDFSIR IYTYADTPDD FQLLNFSLPE EDVKLKIPLI
210 220 230 240 250
HQALKMAQRP VSLFASPWTS PTWLKTNGAV NGKGTLKGHP GDRYHQTWAK
260 270 280 290 300
YFVKFLDAYA EHNLHFWAVT AENEPSAGLF TGYPFQCLGF TPEHQRDFIA
310 320 330 340 350
RDPGPTLANS THRNVRLLML DDQRLLLPHW AQVVLADPEA AKYVHGIAVH
360 370 380 390 400
WYLDFLAPAK ATLGETHRLF PNTMLFASEA CVGSKFWEQS VRLGSWDRGV
410 420 430 440 450
QYSHSIITNL LYHVVGWTDW NLALNPEGGP NWVRNFVDSP IIVDISKDTF
460 470 480 490 500
YKQPMFYHLG HFSKFIPEGS QRVGLAASEK NNLDTVALLR PDGSAVVVVL
510 520 530
NRSSKDVPLT IKDPALGFLE TISPSYSIHT YLWHRK
Length:536
Mass (Da):59,874
Last modified:July 5, 2004 - v1
Checksum:i25F24D3D548D0C9E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ575649 Genomic DNA. Translation: CAE06498.1.
RefSeqiNP_001005730.1. NM_001005730.1.
UniGeneiSsc.45597.

Genome annotation databases

GeneIDi449572.
KEGGissc:449572.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ575649 Genomic DNA. Translation: CAE06498.1 .
RefSeqi NP_001005730.1. NM_001005730.1.
UniGenei Ssc.45597.

3D structure databases

ProteinModelPortali Q70KH2.
SMRi Q70KH2. Positions 40-536.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH30. Glycoside Hydrolase Family 30.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 449572.
KEGGi ssc:449572.

Organism-specific databases

CTDi 2629.

Phylogenomic databases

eggNOGi COG5520.
HOGENOMi HOG000270031.
HOVERGENi HBG002285.
InParanoidi Q70KH2.
KOi K01201.

Enzyme and pathway databases

BRENDAi 3.2.1.45. 6170.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR11069. PTHR11069. 1 hit.
Pfami PF02055. Glyco_hydro_30. 1 hit.
[Graphical view ]
PRINTSi PR00843. GLHYDRLASE30.
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGLCM_PIG
AccessioniPrimary (citable) accession number: Q70KH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3