Reviewed, UniProtKB/Swiss-Prot Q70JN9 (SLDA1_GLUOX)

Names and origin

Protein names

Recommended name:
    Glycerol dehydrogenase large subunit
    EC=1.1.99.22
Alternative name(s):
    Gluconate/polyol dehydrogenase large subunit
    D-sorbitol dehydrogenase subunit sldA
      Short name=SLDH
    D-arabitol dehydrogenase large subunit
      Short name=ARDH

Gene names

Name:	sldA
Synonyms:	ga5dhB
Ordered Locus Names:	GOX0854

Organism

Gluconobacter oxydans (Gluconobacter suboxydans) [Complete proteome] [HAMAP]

Taxonomic identifier

442 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconobacter

Protein attributes

Sequence length	743 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of glycerol to glycerone. Also acts, more slowly, on a number of other polyols including D-sorbitol, D-arabinitol, D-mannitol, meso-erythritol, adonitol and propylene glycol By similarity.
Catalytic activity	Glycerol + acceptor = glycerone + reduced acceptor.
Cofactor	PQQ By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the bacterial PQQ dehydrogenase family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol dehydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 743	719	Glycerol dehydrogenase large subunit		PRO_0000045857

Sequences

	Sequence		Length	Mass (Da)
	Q70JN9-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: FCD88F07B68BAD87	FASTA	743	79,562
	10 20 30 40 50 60 MRRSHLLATV ACATLACAPL AANAQFAPAG SGGSPTSSVP GPGNGSGNSF EPTENTPAAK 70 80 90 100 110 120 SRFSGPSPYA PQAPGVNAAN LPDIGSMDPN DVPQMAPQQS ASPASGDWAA YGHDDSQMRY 130 140 150 160 170 180 SPLSEITPQN ADQLKVAFVY HTGSYPRPGQ TNKWAAETTP IKVGDGLYMC SAQNDIMKID 190 200 210 220 230 240 PATGKEIWRH NINEKYEAIP YTAACKGVTY FTSSQVPEGQ PCHNRILEGT LDMRLIAVDA 250 260 270 280 290 300 ATGNLCEGFG NGGQVNLMQG LGESVPGFVS MTTPPPVVNG VVVVNHEVLD GQRRWAPSGV 310 320 330 340 350 360 IRGYDAESGK FLWAWDVNRP NDHSQPTGNN HYSRGTPNSW AAMTGDNALG LVYVPTGNSA 370 380 390 400 410 420 SDYYSALRSP EENKVSSAVV ALDVKTGSPR WVFQTVHKDV WDYDIGSQAT LMDMPGQDGQ 430 440 450 460 470 480 PVPALIMPTK RGQTFVLDRR DGKPILPVEE RPAPSPGVIP GDPRSPTQPW STGMPALRVP 490 500 510 520 530 540 DLKETDMWGM SPIDQLFCRI KFRRANYTGE FTPPSVDKPW IEYPGYNGGS DWGSVSYDPQ 550 560 570 580 590 600 SGILIANWNI TPMYDQLVTR KKADELGLMP IDDPNYKPGG GGAEGNGAMD GTPYGIVVTP 610 620 630 640 650 660 FWDQYTGMMC NRPPYGMITA IDMKHGQKVL WQHPLGTARA NGPWGLPTGL PWEIGTPNNG 670 680 690 700 710 720 GSVVTAGGVV FIAAATDNQI RAIDEHTGKV VWSAVLPGGG QANPMTYEAN GHQYVAIMAG 730 740 GHHFMMTPVS DQLVVYALPD HKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a gluconate/polyol dehydrogenase gene from Gluconobacter suboxydans IFO 12528, characterisation of the enzyme and its use for the production of 5-ketogluconate in a recombinant Escherichia coli strain." Salusjaervi T., Povelainen M., Hvorslev N., Eneyskaya E.E., Kulminskaya A.A., Shabalin K.A., Neustroev K.N., Kalkkinen N., Miasnikov A.N. Appl. Microbiol. Biotechnol. 65:306-314(2004) [PubMed: 15060755] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 621 / IFO 3172 / NCIB 7069.
[2]	"Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans." Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U. Nat. Biotechnol. 23:195-200(2005) [PubMed: 15665824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 621H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ577472 Genomic DNA. Translation: CAE12058.1. CP000009 Genomic DNA. Translation: AAW60628.1.
RefSeq	YP_191284.1.
3D structure databases
HSSP	HSSP built from PDB template 1KB0 based on UniProtKB Q46444.
ModBase	Search...
Genome annotation databases
GeneID	3250311.
GenomeReviews	Gene locus GOX0854 in contig CP000009_GR.
KEGG	gox:GOX0854.
NMPDR	fig|290633.1.peg.829.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q70JN9.
OMA	Q70JN9. LIANYND.
Enzyme and pathway databases
BioCyc	GOXY290633:GOX0854-MON.
BRENDA	1.1.1.69. 275912. 1.1.99.22. 3050.
Family and domain databases
InterPro	IPR019556. PQQ-dependent_C. IPR019551. PQQ-dependent_N. IPR018391. PQQ_beta_propeller_repeat. IPR002372. PQQ_repeat. IPR011047. Quino_AlcDH-like. IPR001479. Quinoprotein_DH_CS. [Graphical view]
Gene3D	G3DSA:2.140.10.10. Quinoprotein_alc_DH-like. 1 hit.
Pfam	PF01011. PQQ. 3 hits. PF10535. PQQ_C. 1 hit. PF10527. PQQ_N. 1 hit. [Graphical view]
SMART	SM00564. PQQ. 4 hits. [Graphical view]
PROSITE	PS00363. BACTERIAL_PQQ_1. False negative. PS00364. BACTERIAL_PQQ_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SLDA1_GLUOX

Accession

Primary (citable) accession number: Q70JN9
Secondary accession number(s): Q5FSL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)