Q70JA7 (CHSS3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chondroitin sulfate synthase 3
EC=2.4.1.175
EC=2.4.1.226

Alternative name(s):
Carbohydrate synthase 2
Chondroitin glucuronyltransferase 3
Chondroitin synthase 2
Short name=ChSy-2
Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II
N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 3
N-acetylgalactosaminyltransferase 3

Gene names

Name:	CHSY3
Synonyms:	CHSY2, CSS3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	882 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Specific activity is much reduced compared to CHSY1. Ref.1
Catalytic activity	UDP-N-acetyl-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan. UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.
Cofactor	Divalent cations. Highest activities are measured with cobalt, manganese and cadmium. Ref.1
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein Probable Ref.1.
Tissue specificity	Detected at low levels in brain, cerebral cortex, uterus and small intestine. Ref.1
Sequence similarities	Belongs to the chondroitin N-acetylgalactosaminyltransferase family.
Sequence caution	The sequence CAD43233.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Metal-binding
Molecular function	Transferase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity Inferred from electronic annotation. Source: EC glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 882	882	Chondroitin sulfate synthase 3		PRO_0000189562
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	29 – 882	854	Lumenal Potential
	Compositional bias	211 – 235	25	Pro-rich
	Compositional bias	539 – 544	6	Poly-Leu
Sites
	Metal binding	720	1	Divalent metal cation Potential
	Metal binding	834	1	Divalent metal cation Potential
Amino acid modifications
	Modified residue	411	1	Phosphoserine Ref.4
	Modified residue	470	1	Phosphothreonine By similarity
	Glycosylation	155	1	N-linked (GlcNAc...) Potential
	Glycosylation	279	1	N-linked (GlcNAc...) Potential
	Glycosylation	710	1	N-linked (GlcNAc...) Potential
	Glycosylation	878	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	615	1	G → E. Corresponds to variant rs10068403 [ dbSNP | Ensembl ].		VAR_027540
	Natural variant	764	1	D → G. Corresponds to variant rs2015018 [ dbSNP | Ensembl ].		VAR_021174
Experimental info
	Sequence conflict	173	1	L → P in CAE17326. Ref.2
	Sequence conflict	195	1	W → R in CAE17326. Ref.2
	Sequence conflict	202	1	R → H in CAE17326. Ref.2
	Sequence conflict	268	1	G → E in CAE17326. Ref.2
	Sequence conflict	337	1	Y → H in BAC98832. Ref.1
	Sequence conflict	354 – 355	2	GT → RA in BAC98832. Ref.1
	Sequence conflict	361 – 362	2	YE → FQ in BAC98832. Ref.1
	Sequence conflict	856	1	S → N in CAD43233. Ref.2
	Sequence conflict	856	1	S → N in CAE17326. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q70JA7 [UniParc]. Last modified April 17, 2007. Version 3. Checksum: 2FE26485B2CEA515	FASTA	882	100,284
	10 20 30 40 50 60 MAVRSRRPWM SVALGLVLGF TAASWLIAPR VAELSERKRR GSSLCSYYGR SAAGPRAGAQ 70 80 90 100 110 120 QPLPQPQSRP RQEQSPPPAR QDLQGPPLPE AAPGITSFRS SPWQQPPPLQ QRRRGREPEG 130 140 150 160 170 180 ATGLPGAPAA EGEPEEEDGG AAGQRRDGRP GSSHNGSGDG GAAAPSARPR DFLYVGVMTA 190 200 210 220 230 240 QKYLGSRALA AQRTWARFIP GRVEFFSSQQ PPNAGQPPPP LPVIALPGVD DSYPPQKKSF 250 260 270 280 290 300 MMIKYMHDHY LDKYEWFMRA DDDVYIKGDK LEEFLRSLNS SKPLYLGQTG LGNIEELGKL 310 320 330 340 350 360 GLEPGENFCM GGPGMIFSRE VLRRMVPHIG ECLREMYTTH EDVEVGRCVR RFGGTQCVWS 370 380 390 400 410 420 YEMQQLFHEN YEHNRKGYIQ DLHNSKIHAA ITLHPNKRPA YQYRLHNYML SRKISELRYR 430 440 450 460 470 480 TIQLHRESAL MSKLSNTEVS KEDQQLGVIP SFNHFQPRER NEVIEWEFLT GKLLYSAAEN 490 500 510 520 530 540 QPPRQSLSSI LRTALDDTVL QVMEMINENA KSRGRLIDFK EIQYGYRRVN PMHGVEYILD 550 560 570 580 590 600 LLLLYKRHKG RKLTVPVRRH AYLQQLFSKP FFRETEELDV NSLVESINSE TQSFSFISNS 610 620 630 640 650 660 LKILSSFQGA KEMGGHNEKK VHILVPLIGR YDIFLRFMEN FENMCLIPKQ NVKLVIILFS 670 680 690 700 710 720 RDSGQDSSKH IELIKGYQNK YPKAEMTLIP MKGEFSRGLG LEMASAQFDN DTLLLFCDVD 730 740 750 760 770 780 LIFREDFLQR CRDNTIQGQQ VYYPIIFSQY DPKVTNGGNP PTDDYFIFSK KTGFWRDYGY 790 800 810 820 830 840 GITCIYKSDL LGAGGFDTSI QGWGLEDVDL YNKVILSGLR PFRSQEVGVV HIFHPVHCDP 850 860 870 880 NLDPKQYKMC LGSKASTFAS TMQLAELWLE KHLGVRYNRT LS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Chondroitin sulfate synthase-3. Molecular cloning and characterization." Yada T., Sato T., Kaseyama H., Gotoh M., Iwasaki H., Kikuchi N., Kwon Y.-D., Togayachi A., Kudo T., Watanabe H., Narimatsu H., Kimata K. J. Biol. Chem. 278:39711-39725(2003) [PubMed: 12907687] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, COFACTOR, TISSUE SPECIFICITY.
[2]	"A novel gene for chondroitin synthase in 5q23.3." Kamakari S., Chatzidakis I., Anagnou N. Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas and Placenta.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-411, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Chondroitin sulfate synthase 3

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB086062 mRNA. Translation: BAC98832.1. AJ504664 mRNA. Translation: CAD43233.1. Different initiation. AJ578034 mRNA. Translation: CAE17326.1. BC137325 mRNA. Translation: AAI37326.1.
IPI	IPI00418277.
RefSeq	NP_787052.3. NM_175856.4.
UniGene	Hs.213137.
3D structure databases
ProteinModelPortal	Q70JA7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q70JA7.
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31. GT7. Glycosyltransferase Family 7.
PTM databases
PhosphoSite	Q70JA7.
Polymorphism databases
DMDM	145559453.
Proteomic databases
PRIDE	Q70JA7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000305031; ENSP00000302629; ENSG00000198108.
GeneID	337876.
KEGG	hsa:337876.
UCSC	uc003kvd.1. human.
Organism-specific databases
CTD	337876.
GeneCards	GC05P129273.
HGNC	HGNC:24293. CHSY3.
HPA	HPA044612.
MIM	609963. gene.
neXtProt	NX_Q70JA7.
PharmGKB	PA162382259.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00530000062787.
HOGENOM	HBG379060.
HOVERGEN	HBG050948.
InParanoid	Q70JA7.
OMA	DPLHGVE.
OrthoDB	EOG43XV2V.
PhylomeDB	Q70JA7.
Enzyme and pathway databases
BioCyc	MetaCyc:HS11959-MONOMER.
BRENDA	2.4.1.175. 2681.
Gene expression databases
ArrayExpress	Q70JA7.
Bgee	Q70JA7.
CleanEx	HS_CHSY3.
Genevestigator	Q70JA7.
Family and domain databases
InterPro	IPR008428. Chond_GalNAc. [Graphical view]
KO	K13499.
PANTHER	PTHR12369. CHGN. 1 hit.
Pfam	PF05679. CHGN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	96760.
SOURCE	Search...

Entry information

Entry name

CHSS3_HUMAN

Accession

Primary (citable) accession number: Q70JA7
Secondary accession number(s): B2RP97, Q76L22, Q86Y52

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	April 17, 2007
Last modified:	January 25, 2012
This is version 79 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.