Q70GK9 (Q70GK9_STRCT) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
5'-fluoro-5'-deoxy-adenosine synthase EMBL CAJ20006.1

Submitted name:
5'-fluoro-5'-deoxyadenosine synthase EMBL CAE46446.1

Gene names

Name:

flA EMBL CAE46446.1

Organism

Streptomyces cattleya EMBL CAE46446.1

Taxonomic identifier

29303 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine PDB 2V7U PDB 1RQP
Technical term	3D-structure PDB 2C4T PDB 2C4U PDB 2V7T PDB 2CC2 PDB 2V7U PDB 2C5B PDB 2V7V PDB 2V7W PDB 2V7X PDB 2CBX PDB 2C5H PDB 1RQR PDB 1RQP PDB 2C2W
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	21 – 23	3	S-adenosyl-L-homocysteine binding
	Region	21 – 23	3	S-adenosyl-L-methionine 1 binding PDB 2V7U PDB 1RQP
	Region	21 – 23	3	S-adenosyl-L-methionine 2 binding PDB 2V7U
	Region	155 – 156	2	S-adenosyl-L-homocysteine binding
	Region	156 – 158	3	S-adenosyl-L-methionine 1 binding PDB 2V7U PDB 1RQP
	Region	210 – 215	6	S-adenosyl-L-homocysteine binding
	Region	210 – 215	6	S-adenosyl-L-methionine 1 binding PDB 2V7U PDB 1RQP
	Region	210 – 215	6	S-adenosyl-L-methionine 3 binding PDB 2V7U
	Region	269 – 270	2	S-adenosyl-L-homocysteine binding
	Region	269 – 270	2	S-adenosyl-L-methionine 1 binding PDB 2V7U PDB 1RQP
	Region	269 – 270	2	S-adenosyl-L-methionine 3 binding PDB 2V7U
	Region	277 – 279	3	S-adenosyl-L-homocysteine binding
	Region	277 – 279	3	S-adenosyl-L-methionine 1 binding PDB 2V7U PDB 1RQP
	Region	277 – 279	3	S-adenosyl-L-methionine 3 binding PDB 2V7U
Sites
	Binding site	16	1	S-adenosyl-L-homocysteine
	Binding site	16	1	S-adenosyl-L-methionine 1 PDB 2V7U PDB 1RQP
	Binding site	16	1	S-adenosyl-L-methionine 2 PDB 2V7U
	Binding site	50	1	S-adenosyl-L-homocysteine
	Binding site	50	1	S-adenosyl-L-methionine 1 PDB 2V7U PDB 1RQP
	Binding site	50	1	S-adenosyl-L-methionine 2 PDB 2V7U
	Binding site	77	1	S-adenosyl-L-homocysteine; via amide nitrogen
	Binding site	77	1	S-adenosyl-L-methionine 1; via amide nitrogen PDB 2V7U PDB 1RQP
	Binding site	77	1	S-adenosyl-L-methionine 2; via amide nitrogen PDB 2V7U
	Binding site	156	1	S-adenosyl-L-methionine 2 PDB 2V7U
	Binding site	254	1	S-adenosyl-L-homocysteine
	Binding site	254	1	S-adenosyl-L-methionine 1 PDB 2V7U PDB 1RQP
	Binding site	254	1	S-adenosyl-L-methionine 3 PDB 2V7U

Sequences

	Sequence		Length	Mass (Da)
	Q70GK9 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 54697A2F68121731	FASTA	299	32,370
	10 20 30 40 50 60 MAANSTRRPI IAFMSDLGTT DDSVAQCKGL MYSICPDVTV VDVCHSMTPW DVEEGARYIV 70 80 90 100 110 120 DLPRFFPEGT VFATTTYPAT GTTTRSVAVR IKQAAKGGAR GQWAGSGAGF ERAEGSYIYI 130 140 150 160 170 180 APNNGLLTTV LEEHGYLEAY EVTSPKVIPE QPEPTFYSRE MVAIPSAHLA AGFPLSEVGR 190 200 210 220 230 240 PLEDHEIVRF NRPAVEQDGE ALVGVVSAID HPFGNVWTNI HRTDLEKAGI GYGARLRLTL 250 260 270 280 290 DGVLPFEAPL TPTFADAGEI GNIAIYLNSR GYLSIARNAA SLAYPYHLKE GMSARVEAR

References

[1]	"Crystal structure and mechanism of a bacterial fluorinating enzyme." Dong C., Huang F., Deng H., Schaffrath C., Spencer J.B., O'Hagan D., Naismith J.H. Nature 427:561-565(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Substrates and Inhibitors of the Fluorinase from Streptomyces Cattleya." Mcewan A.R., Deng H., Mcglinchey R.P., Robinson D.R., O'Hagan D., Naismith J.H. Submitted (OCT-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS).
[3]	"The fluorinase from Streptomyces cattleya is also a chlorinase." Deng H., Cobb S.L., McEwan A.R., McGlinchey R.P., Naismith J.H., O'Hagan D., Robinson D.A., Spencer J.B. Angew. Chem. Int. Ed. 45:759-762(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS).
[4]	"The gene cluster for fluorometabolite biosynthesis in Streptomyces cattleya: a thioesterase confers resistance to fluoroacetyl-coenzyme A." Huang F., Haydock S.F., Spiteller D., Mironenko T., Li T.L., O'Hagan D., Leadlay P.F., Spencer J.B. Chem. Biol. 13:475-484(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[5]	"Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates." Cobb S.L., Deng H., McEwan A.R., Naismith J.H., O'Hagan D., Robinson D.A. Org. Biomol. Chem. 4:1458-1460(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS).
[6]	"Mechanism of enzymatic fluorination in Streptomyces cattleya." Zhu X., Robinson D.A., McEwan A.R., O'Hagan D., Naismith J.H. J. Am. Chem. Soc. 129:14597-14604(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE AND S-ADENOSYL-L-METHIONINE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ581748 Genomic DNA. Translation: CAE46446.1.
AM055586 Genomic DNA. Translation: CAJ20006.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RQP	X-ray	1.80	A/B/C	1-299	[»]
1RQR	X-ray	2.67	A/B/C	1-299	[»]
2C2W	X-ray	2.00	A/B/C	1-299	[»]
2C4T	X-ray	2.30	A/B/C	1-299	[»]
2C4U	X-ray	2.50	A/B/C/D/E/F	1-299	[»]
2C5B	X-ray	2.50	A/B/C	1-299	[»]
2C5H	X-ray	2.70	A/B/C	1-299	[»]
2CBX	X-ray	2.00	A/B/C	1-299	[»]
2CC2	X-ray	2.00	A/B/C	1-299	[»]
2V7T	X-ray	2.15	A/B/C	1-299	[»]
2V7U	X-ray	2.00	A/B/C	1-299	[»]
2V7V	X-ray	1.94	A/B/C	1-299	[»]
2V7W	X-ray	1.90	A/B/C	1-299	[»]
2V7X	X-ray	1.96	A/B/C	1-299	[»]

SMR

Q70GK9. Positions 8-298.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-15922.

Family and domain databases

Gene3D

2.40.30.90. 1 hit.

InterPro

IPR002747. SAM_OH_AdoTrfase.
IPR023227. SAM_OH_AdoTrfase_C.
IPR023228. SAM_OH_AdoTrfase_N.
[Graphical view]

Pfam

PF01887. SAM_adeno_trans. 2 hits.
[Graphical view]

PIRSF

PIRSF006779. UCP006779. 1 hit.

SUPFAM

SSF101852. SAM_OH_AdoTrfase_C. 1 hit.
SSF102522. SAM_OH_AdoTrfase_N. 1 hit.

ProtoNet

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Other

EvolutionaryTrace

Q70GK9.

Entry information

Entry name

Q70GK9_STRCT

Accession

Primary (citable) accession number: Q70GK9

Entry history

Integrated into UniProtKB/TrEMBL:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)