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Protein

A-kinase anchor protein 9

Gene

Akap9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. Required to maintain the integrity of the Golgi apparatus. Recruited to the Golgi apparatus by GM130/GOLGA2 and is required for microtubule nucleation at the cis-side of the Golgi apparatusGM130/GOLGA2.By similarity

GO - Biological processi

  • microtubule nucleation Source: UniProtKB
  • Sertoli cell development Source: MGI
  • signal transduction Source: InterPro
  • spermatogenesis Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 9
Short name:
AKAP-9
Alternative name(s):
Protein kinase A-anchoring protein 9
Short name:
PRKA9
Gene namesi
Name:Akap9
Synonyms:Kiaa0803
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2178217. Akap9.

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
  • Golgi apparatus By similarity

  • Note: Cytoplasmic in parietal cells.By similarity

GO - Cellular componenti

  • centrosome Source: MGI
  • ciliary basal body Source: MGI
  • cytoplasm Source: MGI
  • Golgi apparatus Source: MGI
  • Golgi stack Source: UniProtKB
  • pericentriolar material Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 37973797A-kinase anchor protein 9PRO_0000395481Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei139 – 1391PhosphoserineBy similarity
Modified residuei1288 – 12881PhosphoserineBy similarity
Modified residuei3732 – 37321PhosphoserineBy similarity
Modified residuei3755 – 37551PhosphoserineBy similarity
Modified residuei3787 – 37871PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ70FJ1.
PaxDbiQ70FJ1.
PeptideAtlasiQ70FJ1.
PRIDEiQ70FJ1.

PTM databases

iPTMnetiQ70FJ1.
PhosphoSiteiQ70FJ1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040407.

Interactioni

Subunit structurei

Interacts with the regulatory region of protein kinase N (PKN), protein phosphatase 2A (PP2A), protein phosphatase 1 (PP1) and the immature non-phosphorylated form of PKC epsilon. Interacts with CIP4 and FNBP1. Interacts with chloride intracellular channel proteins CLIC1, CLIC4 and CLIC5. CSNK1D binding promotes its centrosomal subcellular location. Interacts with GM130/GOLGA2; leading to recruitment to the Golgi apparatus. Interacts with KCNQ1; targets protein kinase A (PKA) catalytic and regulatory subunits and protein phosphatase 1 (PP1), to the heterodimer KCNQ1-KCNE1.By similarity

Protein-protein interaction databases

BioGridi221564. 4 interactions.
IntActiQ70FJ1. 1 interaction.
STRINGi10090.ENSMUSP00000046129.

Structurei

3D structure databases

ProteinModelPortaliQ70FJ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2498 – 251013PKA-RII subunit binding domainBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili140 – 607468Sequence analysisAdd
BLAST
Coiled coili640 – 976337Sequence analysisAdd
BLAST
Coiled coili1808 – 2377570Sequence analysisAdd
BLAST
Coiled coili2975 – 3325351Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi220 – 28162Gln-richAdd
BLAST
Compositional biasi3143 – 3337195Gln-richAdd
BLAST

Domaini

RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410II47. Eukaryota.
ENOG41101YB. LUCA.
HOGENOMiHOG000169582.
HOVERGENiHBG084047.
InParanoidiQ70FJ1.
KOiK16551.
PhylomeDBiQ70FJ1.

Family and domain databases

InterProiIPR028745. AKAP9.
IPR019528. PACT_domain.
[Graphical view]
PANTHERiPTHR18937:SF252. PTHR18937:SF252. 6 hits.
PfamiPF10495. PACT_coil_coil. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q70FJ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDEERQRKL AAGKAKLARF RQRKAQYDGD IPKKQKKKRT SSSKHDSSLH
60 70 80 90 100
TDQQSGELCS ESSQRVDLAG NPDCSGPERK HGQVFSAEPE SEISTTADEC
110 120 130 140 150
SSEINGCNSV MKPRKPTDPL REEEFSLDDS SSEQGAQSSQ TCLQMVEKEL
160 170 180 190 200
AEKQHDIEEL TQELEEMRAS FGTEGLKQLQ EFEAAIKQRD GIITQLTANL
210 220 230 240 250
QQARREKDDT MVEFLELTEQ SQKLQIQFQH LQANETLQNS TLSRTATDLL
260 270 280 290 300
QAKRQIFTQQ QQLQDYQKKE EDLQAQISFL QEKLRAFEME KDRKIENLNA
310 320 330 340 350
KEIQEKQALI DELNTRVVEE EKKTVELKNK VTTADELLGG LHEQLTQRNQ
360 370 380 390 400
EIQSLKLELG NSQQNERKCS EEIKELMRTV EELQKRNLKD SWLETSAVRR
410 420 430 440 450
VEQETQRKLS HLQAELDEMY GKQIVQMKQE LINQHMSQIE ELKSQHKREM
460 470 480 490 500
ENTLKSDTNA AISKEQVNLM NAAINELNVR LQETHAQKEE LKGELGVVLG
510 520 530 540 550
EKSALQSQSN DLLEEVRFLR EQVQKARQTI AEQENRLSEA RKSLSTVEDL
560 570 580 590 600
KAEIVAASES RKELELKHEA EITNYKIKLE MLEKEKNAVL DRMAESQEAE
610 620 630 640 650
LERLRTQPLF SHEEELSKLK EDLEVEHRIN IEKLKDNLGI HYKQQIDGLQ
660 670 680 690 700
NEMNRKMESM QCETDNLITQ QNQLILENSK LRDLQECLVN SKSEEMNLQI
710 720 730 740 750
NELQKEIEIL KQEEKEKGTL EQEVQELQLK TEQLEKQLKE KEDDLQEKCA
760 770 780 790 800
QLDAENNILK EEKRVLEDKL KMYSPSEQEE RSIAVDPSTS KLADSRWQKE
810 820 830 840 850
VAMLRKETED LQQQCLYLNE EIEKQRNTFA FAEKNFEVNY QELQREYTCL
860 870 880 890 900
LKIRDDLEAT QTKQALEYES KLRALEEELL SKRGNPXAPK GKSSGIFPSE
910 920 930 940 950
TLEIGEVVEK DTTELMEKLE VTKREKLELS EKVSGLSEQL KQTHCTINSL
960 970 980 990 1000
SAEXRALKQE KEQLLLRCGE LELLANPSGT ENAAVCPVQM SSYQAGLVMG
1010 1020 1030 1040 1050
KVGDSGGSIS KISKDLAEES KPMIEDKIPF KESGREQLLL PTRAQEPSHA
1060 1070 1080 1090 1100
TVEPCESEKL QQELHALKAE QDDLRLQMEA QRICLFVVYS THADQVRAHM
1110 1120 1130 1140 1150
EKEREEALCS LKDELISAQQ KKIDELHKMH QCQLQNVKIQ ETGDEPLQVL
1160 1170 1180 1190 1200
IERLQKAVSE KCFHISKTLN NVFDECYTPL KCEMNIEEKE NSGVYTSQNQ
1210 1220 1230 1240 1250
SPELQEYRYE VQDFQESMQV LLGKVTEECR KLSGLQTRLG KIHEQQTDGV
1260 1270 1280 1290 1300
ALEFAEQNAA EEEAGLLSGC SQSALQSTDV SLESKVSSLP ASEKNRECER
1310 1320 1330 1340 1350
QVQELQSPVA AGQLQLTETE ASHRAEIECL QQRLEAASEA PVQPSLSIDS
1360 1370 1380 1390 1400
VVFKGSGAQK PVYCGSCLRE YVDGTAKFSD RFEVRQETNM VNLMEKQYQE
1410 1420 1430 1440 1450
RLEEEIAKVI VSMSIAFAQQ TELSRLSEGK ENTIQSEQAH TLCSQNKHQL
1460 1470 1480 1490 1500
NDITSQSQVG LQTFEATDKB FKEEFKPLSK ELGEYRKAVP LSSHDDLDDI
1510 1520 1530 1540 1550
LKSEEHGLAI SEEIFSKDET FIVRKSMHDE VLVSSMDTSR QLILNEQLED
1560 1570 1580 1590 1600
MRQELVRQYE EHQQATEMLR QAHMQQMERQ REDQEQLQEE IKRLNEQLTQ
1610 1620 1630 1640 1650
KSSIDTEHVV SERERVLLEE LEALKQLPLA GRKELCCELR HSSTQTQDGH
1660 1670 1680 1690 1700
DDQEVEEQTL KDKTLERSPE DALLDRNLSN ERYALKKANN RLLKILLEVV
1710 1720 1730 1740 1750
KTTSAAEETI GRHVLGILDR SSKGQTASSL LWRSEADASA TTCAPEDCAR
1760 1770 1780 1790 1800
AMDESIPSYP GTAIATHDSI WSKVTEEGAE LSQRLVRSGF AGPVIDPENE
1810 1820 1830 1840 1850
ELMLNISSRL QAAVEKLLEA ISETNTQLEH AKVTQTELMR ESFRQKQEAT
1860 1870 1880 1890 1900
ESLHCLEELR ERLQEESRAR EQLAEELNKA ESVIDGYSDE KTLFERQIQE
1910 1920 1930 1940 1950
KTDIIEHLEQ EVLCMNNRLQ ELESDQRRVE EERQLLCRQR EAMRAEAGPV
1960 1970 1980 1990 2000
EQQFLQETEK LMKEKLEVQC QAEKVRGDLQ KQVKALEIDV EEQVSRFIEL
2010 2020 2030 2040 2050
EQEKNAELTD LRQQSQALEK QLEKMRKFLD EQAIDREHER DVFQQEIQKL
2060 2070 2080 2090 2100
EHQLKAAPRI QPVSEHQARE VEQLTNHLKE KTDRCSELLL SKEQLQRDIQ
2110 2120 2130 2140 2150
ERNEEIEKLE CRVRELEQAL LASAEPFPKV EDQKRSGAVA ADPELSLEVQ
2160 2170 2180 2190 2200
LQAERDATDR KQKEITNLEE QLEQFREELE NKNDEVQELL MQLEIQRKES
2210 2220 2230 2240 2250
TTRLQELQQE NRLFKDEIEK LGFAMKESDS VSTRDQPMLF GKFAQLIQEK
2260 2270 2280 2290 2300
EIEIDRLNEQ FIKLQQQLKL TTDNKVIEEQ KEQIQDLETQ IERLMSEREH
2310 2320 2330 2340 2350
EKKQREEEVE QLTGVVEKLQ QEVVSTEQQR EGARTLPEDE ESFKHQLDKV
2360 2370 2380 2390 2400
TAEKLVLEQQ VETTNQVMTH MNNVLKEINF KMDQITQSLC NLNKECASNE
2410 2420 2430 2440 2450
ELPSLPKESV HMTVHELGSD NLQPEDAPAQ DVTKPLEKQT SLTRLQESPE
2460 2470 2480 2490 2500
ASRTQEIESL ASSVGAKDVE LTQCREQTET IQEQAQSETD RLQKKLTDLQ
2510 2520 2530 2540 2550
RSLEKFAAAL VSQVQMEAAQ EYVPFHQEKQ PVSSAPGSTD IQNANGLTGA
2560 2570 2580 2590 2600
STESLIPTVT LRLAEVESRV AEVHSGTMSE KLVGIVGGNA SETEKRVIEL
2610 2620 2630 2640 2650
QKLLEEAEER PEEGGEQSSR DGEVRESYMT SLQKDLGQVK DPLTEAKEKL
2660 2670 2680 2690 2700
SYSLEKEKRT GEQESREAPI PEPPSVEVGG CSGLTERTDK VSSSGNQTLQ
2710 2720 2730 2740 2750
ILLRDAAIQT DLQSESSQEE VRDTINQLTK KMEHIQELHA AEILDMESRH
2760 2770 2780 2790 2800
ILETESLKKE HYVAIQLLTK ECETLKEMTQ CLRCKEGSSI PELADSVAYQ
2810 2820 2830 2840 2850
SREVYSSDSE SDWGQSQGFD TAIEGREEGE TSADLFPKKI KGLVKAVHSE
2860 2870 2880 2890 2900
GMQVLSLSSP LCDDGEDRSI QQLSESWLKE RQAYLNTISS LKDLISKMQV
2910 2920 2930 2940 2950
RRETEVYDRC HLSDWRGELL LACQRVFIKE RSVLLATFQT ELTSLSTRDV
2960 2970 2980 2990 3000
DGLLNSLEQR IQEQGIEYHT AMDCLQKADR RSLLAEIEDL RAQINGGKMT
3010 3020 3030 3040 3050
LEREQGTEKS SQELLDCSMQ QKQSLEMQLE LSSLRDRAAE LQEQLSSEKM
3060 3070 3080 3090 3100
VVAELKSELA QAKLELGTTL KAQHKRLKEL EAFRSEVKEK TDEIHFLSDT
3110 3120 3130 3140 3150
LAREQKNSLE LQWALEKEKA RSGHHEEREK EELEDLKFSL EDQKRRNTQL
3160 3170 3180 3190 3200
NLLLEQQKQL LNESQQKIES QKMLHDAQLS EEQGRNLGLQ ALLESEQVRI
3210 3220 3230 3240 3250
QEMKSTLDKE RELYAQLQSR EDGGQPPPAL PSEDLLKELQ KQLEEKHSRI
3260 3270 3280 3290 3300
VELLSETEKY KLDSLQTRQQ MEKDRQVHQK TLQTEQEANT QGQKKMQELQ
3310 3320 3330 3340 3350
SKVEELQRQL QEKRQQVYKL DLEGKRLQGL MQEFQKQELE PEEKPGSRGL
3360 3370 3380 3390 3400
VDQNLNEPAT WNFTDDRTRN WVLQQKMGEA KDRNFTKLIE INGGELDHNH
3410 3420 3430 3440 3450
DLEMIRQTLQ HVASKLQHVA QKACSRLQFE TAGDDAFIWI QENIDGIILQ
3460 3470 3480 3490 3500
LQKLTGQPGD EHSLGPPSSS CGSLTESLMR QNTELTRLIN QLTEEKNTLR
3510 3520 3530 3540 3550
SIVIKLEELN RCYWHTGASR DCCSRFSFID PADIEAIIAS EKEVWNREKL
3560 3570 3580 3590 3600
SLQKALKRAE AKVYKLKAEL RNDALLRNLG PDTDHAALQK IYNKYLRASS
3610 3620 3630 3640 3650
FRKALIYQKK YLLLLLGGFQ ECEDVTLGVL ARMGGHLALK DSKTITNHPK
3660 3670 3680 3690 3700
AFSRFRSAVR VSIAISRMKF LVRRWQQVTS TSSININRDG FGLSPGIEKT
3710 3720 3730 3740 3750
DPFYHSPGGL ELYGEPRHTM YRSRFDLDYP RSLLPLQNRY PGTPGDLNSI
3760 3770 3780 3790
SMASSQLHQY NPDKSLTDYV TRLEALRRRL GAIQSGSTTQ FHFGMRR
Length:3,797
Mass (Da):436,214
Last modified:July 13, 2010 - v2
Checksum:iCA30D11FF5696124
GO
Isoform 2 (identifier: Q70FJ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     104-121: Missing.

Note: Incomplete sequence.
Show »
Length:3,779
Mass (Da):434,206
Checksum:i52DE20D0FB7DAE3E
GO
Isoform 3 (identifier: Q70FJ1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     3436-3454: Missing.

Note: Incomplete sequence.
Show »
Length:3,778
Mass (Da):433,976
Checksum:iEC4EB9FA61875834
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621S → N in CAE46960 (Ref. 1) Curated
Sequence conflicti2388 – 23881S → L in CAE46960 (Ref. 1) Curated
Sequence conflicti2775 – 27751L → Q in CAE46960 (Ref. 1) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei104 – 12118Missing in isoform 2. 1 PublicationVSP_039481Add
BLAST
Alternative sequencei3436 – 345419Missing in isoform 3. 1 PublicationVSP_039482Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ582913 mRNA. Translation: CAE46960.1.
AK122374 Transcribed RNA. Translation: BAC65656.2.
AK168404 mRNA. Translation: BAE40324.1.
CCDSiCCDS19070.1. [Q70FJ1-2]
RefSeqiNP_919444.2. NM_194462.2.
UniGeneiMm.46044.

Genome annotation databases

GeneIDi100986.
KEGGimmu:100986.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ582913 mRNA. Translation: CAE46960.1.
AK122374 Transcribed RNA. Translation: BAC65656.2.
AK168404 mRNA. Translation: BAE40324.1.
CCDSiCCDS19070.1. [Q70FJ1-2]
RefSeqiNP_919444.2. NM_194462.2.
UniGeneiMm.46044.

3D structure databases

ProteinModelPortaliQ70FJ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221564. 4 interactions.
IntActiQ70FJ1. 1 interaction.
STRINGi10090.ENSMUSP00000046129.

PTM databases

iPTMnetiQ70FJ1.
PhosphoSiteiQ70FJ1.

Proteomic databases

MaxQBiQ70FJ1.
PaxDbiQ70FJ1.
PeptideAtlasiQ70FJ1.
PRIDEiQ70FJ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100986.
KEGGimmu:100986.

Organism-specific databases

CTDi10142.
MGIiMGI:2178217. Akap9.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410II47. Eukaryota.
ENOG41101YB. LUCA.
HOGENOMiHOG000169582.
HOVERGENiHBG084047.
InParanoidiQ70FJ1.
KOiK16551.
PhylomeDBiQ70FJ1.

Miscellaneous databases

ChiTaRSiAkap9. mouse.
PROiQ70FJ1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040407.

Family and domain databases

InterProiIPR028745. AKAP9.
IPR019528. PACT_domain.
[Graphical view]
PANTHERiPTHR18937:SF252. PTHR18937:SF252. 6 hits.
PfamiPF10495. PACT_coil_coil. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAKAP9_MOUSE
AccessioniPrimary (citable) accession number: Q70FJ1
Secondary accession number(s): Q3TH74, Q80TR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 13, 2010
Last modified: September 7, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.