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Q70EL4

- UBP43_HUMAN

UniProt

Q70EL4 - UBP43_HUMAN

Protein

Ubiquitin carboxyl-terminal hydrolase 43

Gene

USP43

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (18 May 2010)
      Previous versions | rss
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    Functioni

    May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei110 – 1101NucleophilePROSITE-ProRule annotation
    Active sitei668 – 6681Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.976.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 43 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 43
    Ubiquitin thioesterase 43
    Ubiquitin-specific-processing protease 43
    Gene namesi
    Name:USP43
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:20072. USP43.

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134865304.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11231123Ubiquitin carboxyl-terminal hydrolase 43PRO_0000249521Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1041 – 10411Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ70EL4.
    PaxDbiQ70EL4.
    PRIDEiQ70EL4.

    PTM databases

    PhosphoSiteiQ70EL4.

    Expressioni

    Tissue specificityi

    Expressed in brain, aorta and lung at low levels.1 Publication

    Gene expression databases

    ArrayExpressiQ70EL4.
    BgeeiQ70EL4.
    CleanExiHS_USP43.
    GenevestigatoriQ70EL4.

    Organism-specific databases

    HPAiHPA023389.
    HPA027762.

    Interactioni

    Protein-protein interaction databases

    BioGridi125884. 9 interactions.
    IntActiQ70EL4. 10 interactions.
    MINTiMINT-3976419.
    STRINGi9606.ENSP00000285199.

    Structurei

    3D structure databases

    ProteinModelPortaliQ70EL4.
    SMRiQ70EL4. Positions 99-273, 539-711.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini101 – 710610USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5560.
    HOGENOMiHOG000293426.
    HOVERGENiHBG094126.
    InParanoidiQ70EL4.
    KOiK11856.
    OMAiWQQQQAH.
    OrthoDBiEOG7VMP4B.
    PhylomeDBiQ70EL4.
    TreeFamiTF106278.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q70EL4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDLGPGDAAG GGPLAPRPRR RRSLRRLFSR FLLALGSRSR PGDSPPRPQP     50
    GHCDGDGEGG FACAPGPVPA APGSPGEERP PGPQPQLQLP AGDGARPPGA 100
    QGLKNHGNTC FMNAVVQCLS NTDLLAEFLA LGRYRAAPGR AEVTEQLAAL 150
    VRALWTREYT PQLSAEFKNA VSKYGSQFQG NSQHDALEFL LWLLDRVHED 200
    LEGSSRGPVS EKLPPEATKT SENCLSPSAQ LPLGQSFVQS HFQAQYRSSL 250
    TCPHCLKQSN TFDPFLCVSL PIPLRQTRFL SVTLVFPSKS QRFLRVGLAV 300
    PILSTVAALR KMVAEEGGVP ADEVILVELY PSGFQRSFFD EEDLNTIAEG 350
    DNVYAFQVPP SPSQGTLSAH PLGLSASPRL AAREGQRFSL SLHSESKVLI 400
    LFCNLVGSGQ QASRFGPPFL IREDRAVSWA QLQQSILSKV RHLMKSEAPV 450
    QNLGSLFSIR VVGLSVACSY LSPKDSRPLC HWAVDRVLHL RRPGGPPHVK 500
    LAVEWDSSVK ERLFGSLQEE RAQDADSVWQ QQQAHQQHSC TLDECFQFYT 550
    KEEQLAQDDA WKCPHCQVLQ QGMVKLSLWT LPDILIIHLK RFCQVGERRN 600
    KLSTLVKFPL SGLNMAPHVA QRSTSPEAGL GPWPSWKQPD CLPTSYPLDF 650
    LYDLYAVCNH HGNLQGGHYT AYCRNSLDGQ WYSYDDSTVE PLREDEVNTR 700
    GAYILFYQKR NSIPPWSASS SMRGSTSSSL SDHWLLRLGS HAGSTRGSLL 750
    SWSSAPCPSL PQVPDSPIFT NSLCNQEKGG LEPRRLVRGV KGRSISMKAP 800
    TTSRAKQGPF KTMPLRWSFG SKEKPPGASV ELVEYLESRR RPRSTSQSIV 850
    SLLTGTAGED EKSASPRSNV ALPANSEDGG RAIERGPAGV PCPSAQPNHC 900
    LAPGNSDGPN TARKLKENAG QDIKLPRKFD LPLTVMPSVE HEKPARPEGQ 950
    KAMNWKESFQ MGSKSSPPSP YMGFSGNSKD SRRGTSELDR PLQGTLTLLR 1000
    SVFRKKENRR NERAEVSPQV PPVSLVSGGL SPAMDGQAPG SPPALRIPEG 1050
    LARGLGSRLE RDVWSAPSSL RLPRKASRAP RGSALGMSQR TVPGEQASYG 1100
    TFQRVKYHTL SLGRKKTLPE SSF 1123
    Length:1,123
    Mass (Da):122,809
    Last modified:May 18, 2010 - v2
    Checksum:i3B4FE86EC619173C
    GO
    Isoform 2 (identifier: Q70EL4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-488: Missing.
         489-553: HLRRPGGPPH...ECFQFYTKEE → MPTVCGSSSR...GASQSGWFAF

    Note: No experimental confirmation available.

    Show »
    Length:635
    Mass (Da):69,345
    Checksum:iEF610C195CD3F51D
    GO
    Isoform 3 (identifier: Q70EL4-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-311: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:812
    Mass (Da):89,337
    Checksum:i9E49012662FF0655
    GO
    Isoform 4 (identifier: Q70EL4-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         666-670: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,118
    Mass (Da):122,294
    Checksum:iC1949C4BBF6E8698
    GO

    Sequence cautioni

    The sequence BAB70869.1 differs from that shown. Reason: Frameshift at position 619.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti168 – 1681K → FQ in CAE47744. (PubMed:14715245)Curated
    Sequence conflicti212 – 2121K → Q in CAE47744. (PubMed:14715245)Curated
    Sequence conflicti976 – 9761G → E in BAB70869. (PubMed:14702039)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 488488Missing in isoform 2. 1 PublicationVSP_020466Add
    BLAST
    Alternative sequencei1 – 311311Missing in isoform 3. 1 PublicationVSP_020467Add
    BLAST
    Alternative sequencei489 – 55365HLRRP…YTKEE → MPTVCGSSSRRISSTAVPWM NVFSSTPRRSRSRPGGPCPG REGGWLPLVGWPRAAGASQS GWFAF in isoform 2. 1 PublicationVSP_020468Add
    BLAST
    Alternative sequencei666 – 6705Missing in isoform 4. 1 PublicationVSP_046779

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ583817 mRNA. Translation: CAE47744.2.
    AK055188 mRNA. Translation: BAB70869.1. Frameshift.
    AK090821 mRNA. No translation available.
    AC027045 Genomic DNA. No translation available.
    AC118755 Genomic DNA. No translation available.
    CH471108 Genomic DNA. Translation: EAW90022.1.
    BC136368 mRNA. Translation: AAI36369.1.
    BC144041 mRNA. Translation: AAI44042.1.
    BC171759 mRNA. Translation: AAI71759.1.
    CCDSiCCDS45610.1. [Q70EL4-1]
    CCDS58516.1. [Q70EL4-4]
    RefSeqiNP_001254505.1. NM_001267576.1. [Q70EL4-4]
    NP_694942.3. NM_153210.4. [Q70EL4-1]
    UniGeneiHs.709621.

    Genome annotation databases

    EnsembliENST00000285199; ENSP00000285199; ENSG00000154914. [Q70EL4-1]
    ENST00000570475; ENSP00000458963; ENSG00000154914. [Q70EL4-4]
    GeneIDi124739.
    KEGGihsa:124739.
    UCSCiuc002gma.4. human. [Q70EL4-1]
    uc002gmc.4. human. [Q70EL4-2]
    uc010vva.3. human.

    Polymorphism databases

    DMDMi296452852.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ583817 mRNA. Translation: CAE47744.2 .
    AK055188 mRNA. Translation: BAB70869.1 . Frameshift.
    AK090821 mRNA. No translation available.
    AC027045 Genomic DNA. No translation available.
    AC118755 Genomic DNA. No translation available.
    CH471108 Genomic DNA. Translation: EAW90022.1 .
    BC136368 mRNA. Translation: AAI36369.1 .
    BC144041 mRNA. Translation: AAI44042.1 .
    BC171759 mRNA. Translation: AAI71759.1 .
    CCDSi CCDS45610.1. [Q70EL4-1 ]
    CCDS58516.1. [Q70EL4-4 ]
    RefSeqi NP_001254505.1. NM_001267576.1. [Q70EL4-4 ]
    NP_694942.3. NM_153210.4. [Q70EL4-1 ]
    UniGenei Hs.709621.

    3D structure databases

    ProteinModelPortali Q70EL4.
    SMRi Q70EL4. Positions 99-273, 539-711.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 125884. 9 interactions.
    IntActi Q70EL4. 10 interactions.
    MINTi MINT-3976419.
    STRINGi 9606.ENSP00000285199.

    Protein family/group databases

    MEROPSi C19.976.

    PTM databases

    PhosphoSitei Q70EL4.

    Polymorphism databases

    DMDMi 296452852.

    Proteomic databases

    MaxQBi Q70EL4.
    PaxDbi Q70EL4.
    PRIDEi Q70EL4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000285199 ; ENSP00000285199 ; ENSG00000154914 . [Q70EL4-1 ]
    ENST00000570475 ; ENSP00000458963 ; ENSG00000154914 . [Q70EL4-4 ]
    GeneIDi 124739.
    KEGGi hsa:124739.
    UCSCi uc002gma.4. human. [Q70EL4-1 ]
    uc002gmc.4. human. [Q70EL4-2 ]
    uc010vva.3. human.

    Organism-specific databases

    CTDi 124739.
    GeneCardsi GC17P009489.
    H-InvDB HIX0013531.
    HGNCi HGNC:20072. USP43.
    HPAi HPA023389.
    HPA027762.
    neXtProti NX_Q70EL4.
    PharmGKBi PA134865304.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5560.
    HOGENOMi HOG000293426.
    HOVERGENi HBG094126.
    InParanoidi Q70EL4.
    KOi K11856.
    OMAi WQQQQAH.
    OrthoDBi EOG7VMP4B.
    PhylomeDBi Q70EL4.
    TreeFami TF106278.

    Miscellaneous databases

    GenomeRNAii 124739.
    NextBioi 81362.
    PROi Q70EL4.

    Gene expression databases

    ArrayExpressi Q70EL4.
    Bgeei Q70EL4.
    CleanExi HS_USP43.
    Genevestigatori Q70EL4.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases."
      Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S., Lopez-Otin C.
      Biochem. Biophys. Res. Commun. 314:54-62(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
      Tissue: Amygdala.
    3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
      Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
      , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
      Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
      Tissue: Testis.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1041, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiUBP43_HUMAN
    AccessioniPrimary (citable) accession number: Q70EL4
    Secondary accession number(s): A6NDT9
    , B7ZLT9, B7ZVX5, Q8N2C5, Q96DQ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2006
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3