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Protein

Ubiquitin carboxyl-terminal hydrolase 43

Gene

USP43

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei110 – 1101NucleophilePROSITE-ProRule annotation
Active sitei668 – 6681Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: GO_Central
  2. ubiquitin-specific protease activity Source: GO_Central

GO - Biological processi

  1. proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  2. protein deubiquitination Source: GO_Central
  3. regulation of proteasomal protein catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 43 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 43
Ubiquitin thioesterase 43
Ubiquitin-specific-processing protease 43
Gene namesi
Name:USP43
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:20072. USP43.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134865304.

Polymorphism and mutation databases

BioMutaiUSP43.
DMDMi296452852.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11231123Ubiquitin carboxyl-terminal hydrolase 43PRO_0000249521Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1041 – 10411Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ70EL4.
PaxDbiQ70EL4.
PRIDEiQ70EL4.

PTM databases

PhosphoSiteiQ70EL4.

Expressioni

Tissue specificityi

Expressed in brain, aorta and lung at low levels.1 Publication

Gene expression databases

BgeeiQ70EL4.
CleanExiHS_USP43.
ExpressionAtlasiQ70EL4. baseline and differential.
GenevestigatoriQ70EL4.

Organism-specific databases

HPAiHPA023389.
HPA027762.

Interactioni

Protein-protein interaction databases

BioGridi125884. 9 interactions.
IntActiQ70EL4. 10 interactions.
MINTiMINT-3976419.
STRINGi9606.ENSP00000285199.

Structurei

3D structure databases

ProteinModelPortaliQ70EL4.
SMRiQ70EL4. Positions 99-273, 539-711.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini101 – 710610USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5560.
GeneTreeiENSGT00670000097750.
HOGENOMiHOG000293426.
HOVERGENiHBG094126.
InParanoidiQ70EL4.
KOiK11856.
OMAiQQCILSK.
OrthoDBiEOG7VMP4B.
PhylomeDBiQ70EL4.
TreeFamiTF106278.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q70EL4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLGPGDAAG GGPLAPRPRR RRSLRRLFSR FLLALGSRSR PGDSPPRPQP
60 70 80 90 100
GHCDGDGEGG FACAPGPVPA APGSPGEERP PGPQPQLQLP AGDGARPPGA
110 120 130 140 150
QGLKNHGNTC FMNAVVQCLS NTDLLAEFLA LGRYRAAPGR AEVTEQLAAL
160 170 180 190 200
VRALWTREYT PQLSAEFKNA VSKYGSQFQG NSQHDALEFL LWLLDRVHED
210 220 230 240 250
LEGSSRGPVS EKLPPEATKT SENCLSPSAQ LPLGQSFVQS HFQAQYRSSL
260 270 280 290 300
TCPHCLKQSN TFDPFLCVSL PIPLRQTRFL SVTLVFPSKS QRFLRVGLAV
310 320 330 340 350
PILSTVAALR KMVAEEGGVP ADEVILVELY PSGFQRSFFD EEDLNTIAEG
360 370 380 390 400
DNVYAFQVPP SPSQGTLSAH PLGLSASPRL AAREGQRFSL SLHSESKVLI
410 420 430 440 450
LFCNLVGSGQ QASRFGPPFL IREDRAVSWA QLQQSILSKV RHLMKSEAPV
460 470 480 490 500
QNLGSLFSIR VVGLSVACSY LSPKDSRPLC HWAVDRVLHL RRPGGPPHVK
510 520 530 540 550
LAVEWDSSVK ERLFGSLQEE RAQDADSVWQ QQQAHQQHSC TLDECFQFYT
560 570 580 590 600
KEEQLAQDDA WKCPHCQVLQ QGMVKLSLWT LPDILIIHLK RFCQVGERRN
610 620 630 640 650
KLSTLVKFPL SGLNMAPHVA QRSTSPEAGL GPWPSWKQPD CLPTSYPLDF
660 670 680 690 700
LYDLYAVCNH HGNLQGGHYT AYCRNSLDGQ WYSYDDSTVE PLREDEVNTR
710 720 730 740 750
GAYILFYQKR NSIPPWSASS SMRGSTSSSL SDHWLLRLGS HAGSTRGSLL
760 770 780 790 800
SWSSAPCPSL PQVPDSPIFT NSLCNQEKGG LEPRRLVRGV KGRSISMKAP
810 820 830 840 850
TTSRAKQGPF KTMPLRWSFG SKEKPPGASV ELVEYLESRR RPRSTSQSIV
860 870 880 890 900
SLLTGTAGED EKSASPRSNV ALPANSEDGG RAIERGPAGV PCPSAQPNHC
910 920 930 940 950
LAPGNSDGPN TARKLKENAG QDIKLPRKFD LPLTVMPSVE HEKPARPEGQ
960 970 980 990 1000
KAMNWKESFQ MGSKSSPPSP YMGFSGNSKD SRRGTSELDR PLQGTLTLLR
1010 1020 1030 1040 1050
SVFRKKENRR NERAEVSPQV PPVSLVSGGL SPAMDGQAPG SPPALRIPEG
1060 1070 1080 1090 1100
LARGLGSRLE RDVWSAPSSL RLPRKASRAP RGSALGMSQR TVPGEQASYG
1110 1120
TFQRVKYHTL SLGRKKTLPE SSF
Length:1,123
Mass (Da):122,809
Last modified:May 18, 2010 - v2
Checksum:i3B4FE86EC619173C
GO
Isoform 2 (identifier: Q70EL4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-488: Missing.
     489-553: HLRRPGGPPH...ECFQFYTKEE → MPTVCGSSSR...GASQSGWFAF

Note: No experimental confirmation available.

Show »
Length:635
Mass (Da):69,345
Checksum:iEF610C195CD3F51D
GO
Isoform 3 (identifier: Q70EL4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-311: Missing.

Note: No experimental confirmation available.

Show »
Length:812
Mass (Da):89,337
Checksum:i9E49012662FF0655
GO
Isoform 4 (identifier: Q70EL4-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     666-670: Missing.

Note: No experimental confirmation available.

Show »
Length:1,118
Mass (Da):122,294
Checksum:iC1949C4BBF6E8698
GO

Sequence cautioni

The sequence BAB70869.1 differs from that shown. Reason: Frameshift at position 619. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti168 – 1681K → FQ in CAE47744 (PubMed:14715245).Curated
Sequence conflicti212 – 2121K → Q in CAE47744 (PubMed:14715245).Curated
Sequence conflicti976 – 9761G → E in BAB70869 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 488488Missing in isoform 2. 1 PublicationVSP_020466Add
BLAST
Alternative sequencei1 – 311311Missing in isoform 3. 1 PublicationVSP_020467Add
BLAST
Alternative sequencei489 – 55365HLRRP…YTKEE → MPTVCGSSSRRISSTAVPWM NVFSSTPRRSRSRPGGPCPG REGGWLPLVGWPRAAGASQS GWFAF in isoform 2. 1 PublicationVSP_020468Add
BLAST
Alternative sequencei666 – 6705Missing in isoform 4. 1 PublicationVSP_046779

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ583817 mRNA. Translation: CAE47744.2.
AK055188 mRNA. Translation: BAB70869.1. Frameshift.
AK090821 mRNA. No translation available.
AC027045 Genomic DNA. No translation available.
AC118755 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90022.1.
BC136368 mRNA. Translation: AAI36369.1.
BC144041 mRNA. Translation: AAI44042.1.
BC171759 mRNA. Translation: AAI71759.1.
CCDSiCCDS45610.1. [Q70EL4-1]
CCDS58516.1. [Q70EL4-4]
RefSeqiNP_001254505.1. NM_001267576.1. [Q70EL4-4]
NP_694942.3. NM_153210.4. [Q70EL4-1]
UniGeneiHs.709621.

Genome annotation databases

EnsembliENST00000285199; ENSP00000285199; ENSG00000154914. [Q70EL4-1]
ENST00000570475; ENSP00000458963; ENSG00000154914. [Q70EL4-4]
GeneIDi124739.
KEGGihsa:124739.
UCSCiuc002gma.4. human. [Q70EL4-1]
uc002gmc.4. human. [Q70EL4-2]
uc010vva.3. human.

Polymorphism and mutation databases

BioMutaiUSP43.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ583817 mRNA. Translation: CAE47744.2.
AK055188 mRNA. Translation: BAB70869.1. Frameshift.
AK090821 mRNA. No translation available.
AC027045 Genomic DNA. No translation available.
AC118755 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90022.1.
BC136368 mRNA. Translation: AAI36369.1.
BC144041 mRNA. Translation: AAI44042.1.
BC171759 mRNA. Translation: AAI71759.1.
CCDSiCCDS45610.1. [Q70EL4-1]
CCDS58516.1. [Q70EL4-4]
RefSeqiNP_001254505.1. NM_001267576.1. [Q70EL4-4]
NP_694942.3. NM_153210.4. [Q70EL4-1]
UniGeneiHs.709621.

3D structure databases

ProteinModelPortaliQ70EL4.
SMRiQ70EL4. Positions 99-273, 539-711.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125884. 9 interactions.
IntActiQ70EL4. 10 interactions.
MINTiMINT-3976419.
STRINGi9606.ENSP00000285199.

Protein family/group databases

MEROPSiC19.976.

PTM databases

PhosphoSiteiQ70EL4.

Polymorphism and mutation databases

BioMutaiUSP43.
DMDMi296452852.

Proteomic databases

MaxQBiQ70EL4.
PaxDbiQ70EL4.
PRIDEiQ70EL4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000285199; ENSP00000285199; ENSG00000154914. [Q70EL4-1]
ENST00000570475; ENSP00000458963; ENSG00000154914. [Q70EL4-4]
GeneIDi124739.
KEGGihsa:124739.
UCSCiuc002gma.4. human. [Q70EL4-1]
uc002gmc.4. human. [Q70EL4-2]
uc010vva.3. human.

Organism-specific databases

CTDi124739.
GeneCardsiGC17P009548.
H-InvDBHIX0013531.
HGNCiHGNC:20072. USP43.
HPAiHPA023389.
HPA027762.
neXtProtiNX_Q70EL4.
PharmGKBiPA134865304.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5560.
GeneTreeiENSGT00670000097750.
HOGENOMiHOG000293426.
HOVERGENiHBG094126.
InParanoidiQ70EL4.
KOiK11856.
OMAiQQCILSK.
OrthoDBiEOG7VMP4B.
PhylomeDBiQ70EL4.
TreeFamiTF106278.

Miscellaneous databases

GenomeRNAii124739.
NextBioi81362.
PROiQ70EL4.

Gene expression databases

BgeeiQ70EL4.
CleanExiHS_USP43.
ExpressionAtlasiQ70EL4. baseline and differential.
GenevestigatoriQ70EL4.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases."
    Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S., Lopez-Otin C.
    Biochem. Biophys. Res. Commun. 314:54-62(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Tissue: Amygdala.
  3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
    Tissue: Testis.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1041, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiUBP43_HUMAN
AccessioniPrimary (citable) accession number: Q70EL4
Secondary accession number(s): A6NDT9
, B7ZLT9, B7ZVX5, Q8N2C5, Q96DQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: May 18, 2010
Last modified: April 29, 2015
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.