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Q70EL4 (UBP43_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 43

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 43
Ubiquitin thioesterase 43
Ubiquitin-specific-processing protease 43
Gene names
Name:USP43
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1123 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Tissue specificity

Expressed in brain, aorta and lung at low levels. Ref.1

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Sequence caution

The sequence BAB70869.1 differs from that shown. Reason: Frameshift at position 619.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Coding sequence diversityAlternative splicing
   Molecular functionHydrolase
Protease
Thiol protease
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q70EL4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q70EL4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-488: Missing.
     489-553: HLRRPGGPPH...ECFQFYTKEE → MPTVCGSSSR...GASQSGWFAF
Note: No experimental confirmation available.
Isoform 3 (identifier: Q70EL4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-311: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q70EL4-4)

The sequence of this isoform differs from the canonical sequence as follows:
     666-670: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11231123Ubiquitin carboxyl-terminal hydrolase 43
PRO_0000249521

Regions

Domain101 – 710610USP

Sites

Active site1101Nucleophile By similarity
Active site6681Proton acceptor By similarity

Amino acid modifications

Modified residue10411Phosphoserine Ref.6

Natural variations

Alternative sequence1 – 488488Missing in isoform 2.
VSP_020466
Alternative sequence1 – 311311Missing in isoform 3.
VSP_020467
Alternative sequence489 – 55365HLRRP…YTKEE → MPTVCGSSSRRISSTAVPWM NVFSSTPRRSRSRPGGPCPG REGGWLPLVGWPRAAGASQS GWFAF in isoform 2.
VSP_020468
Alternative sequence666 – 6705Missing in isoform 4.
VSP_046779

Experimental info

Sequence conflict1681K → FQ in CAE47744. Ref.1
Sequence conflict2121K → Q in CAE47744. Ref.1
Sequence conflict9761G → E in BAB70869. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 18, 2010. Version 2.
Checksum: 3B4FE86EC619173C

FASTA1,123122,809
        10         20         30         40         50         60 
MDLGPGDAAG GGPLAPRPRR RRSLRRLFSR FLLALGSRSR PGDSPPRPQP GHCDGDGEGG 

        70         80         90        100        110        120 
FACAPGPVPA APGSPGEERP PGPQPQLQLP AGDGARPPGA QGLKNHGNTC FMNAVVQCLS 

       130        140        150        160        170        180 
NTDLLAEFLA LGRYRAAPGR AEVTEQLAAL VRALWTREYT PQLSAEFKNA VSKYGSQFQG 

       190        200        210        220        230        240 
NSQHDALEFL LWLLDRVHED LEGSSRGPVS EKLPPEATKT SENCLSPSAQ LPLGQSFVQS 

       250        260        270        280        290        300 
HFQAQYRSSL TCPHCLKQSN TFDPFLCVSL PIPLRQTRFL SVTLVFPSKS QRFLRVGLAV 

       310        320        330        340        350        360 
PILSTVAALR KMVAEEGGVP ADEVILVELY PSGFQRSFFD EEDLNTIAEG DNVYAFQVPP 

       370        380        390        400        410        420 
SPSQGTLSAH PLGLSASPRL AAREGQRFSL SLHSESKVLI LFCNLVGSGQ QASRFGPPFL 

       430        440        450        460        470        480 
IREDRAVSWA QLQQSILSKV RHLMKSEAPV QNLGSLFSIR VVGLSVACSY LSPKDSRPLC 

       490        500        510        520        530        540 
HWAVDRVLHL RRPGGPPHVK LAVEWDSSVK ERLFGSLQEE RAQDADSVWQ QQQAHQQHSC 

       550        560        570        580        590        600 
TLDECFQFYT KEEQLAQDDA WKCPHCQVLQ QGMVKLSLWT LPDILIIHLK RFCQVGERRN 

       610        620        630        640        650        660 
KLSTLVKFPL SGLNMAPHVA QRSTSPEAGL GPWPSWKQPD CLPTSYPLDF LYDLYAVCNH 

       670        680        690        700        710        720 
HGNLQGGHYT AYCRNSLDGQ WYSYDDSTVE PLREDEVNTR GAYILFYQKR NSIPPWSASS 

       730        740        750        760        770        780 
SMRGSTSSSL SDHWLLRLGS HAGSTRGSLL SWSSAPCPSL PQVPDSPIFT NSLCNQEKGG 

       790        800        810        820        830        840 
LEPRRLVRGV KGRSISMKAP TTSRAKQGPF KTMPLRWSFG SKEKPPGASV ELVEYLESRR 

       850        860        870        880        890        900 
RPRSTSQSIV SLLTGTAGED EKSASPRSNV ALPANSEDGG RAIERGPAGV PCPSAQPNHC 

       910        920        930        940        950        960 
LAPGNSDGPN TARKLKENAG QDIKLPRKFD LPLTVMPSVE HEKPARPEGQ KAMNWKESFQ 

       970        980        990       1000       1010       1020 
MGSKSSPPSP YMGFSGNSKD SRRGTSELDR PLQGTLTLLR SVFRKKENRR NERAEVSPQV 

      1030       1040       1050       1060       1070       1080 
PPVSLVSGGL SPAMDGQAPG SPPALRIPEG LARGLGSRLE RDVWSAPSSL RLPRKASRAP 

      1090       1100       1110       1120 
RGSALGMSQR TVPGEQASYG TFQRVKYHTL SLGRKKTLPE SSF 

« Hide

Isoform 2 [UniParc].

Checksum: EF610C195CD3F51D
Show »

FASTA63569,345
Isoform 3 [UniParc].

Checksum: 9E49012662FF0655
Show »

FASTA81289,337
Isoform 4 [UniParc].

Checksum: C1949C4BBF6E8698
Show »

FASTA1,118122,294

References

« Hide 'large scale' references
[1]"Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases."
Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S., Lopez-Otin C.
Biochem. Biophys. Res. Commun. 314:54-62(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Amygdala.
[3]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
Tissue: Testis.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1041, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ583817 mRNA. Translation: CAE47744.2.
AK055188 mRNA. Translation: BAB70869.1. Frameshift.
AK090821 mRNA. No translation available.
AC027045 Genomic DNA. No translation available.
AC118755 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90022.1.
BC136368 mRNA. Translation: AAI36369.1.
BC144041 mRNA. Translation: AAI44042.1.
BC171759 mRNA. Translation: AAI71759.1.
CCDSCCDS45610.1. [Q70EL4-1]
CCDS58516.1. [Q70EL4-4]
RefSeqNP_001254505.1. NM_001267576.1. [Q70EL4-4]
NP_694942.3. NM_153210.4. [Q70EL4-1]
UniGeneHs.709621.

3D structure databases

ProteinModelPortalQ70EL4.
SMRQ70EL4. Positions 99-273, 539-711.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125884. 9 interactions.
IntActQ70EL4. 10 interactions.
MINTMINT-3976419.
STRING9606.ENSP00000285199.

Protein family/group databases

MEROPSC19.976.

PTM databases

PhosphoSiteQ70EL4.

Polymorphism databases

DMDM296452852.

Proteomic databases

MaxQBQ70EL4.
PaxDbQ70EL4.
PRIDEQ70EL4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000285199; ENSP00000285199; ENSG00000154914. [Q70EL4-1]
ENST00000570475; ENSP00000458963; ENSG00000154914. [Q70EL4-4]
GeneID124739.
KEGGhsa:124739.
UCSCuc002gma.4. human. [Q70EL4-1]
uc002gmc.4. human. [Q70EL4-2]
uc010vva.3. human.

Organism-specific databases

CTD124739.
GeneCardsGC17P009489.
H-InvDBHIX0013531.
HGNCHGNC:20072. USP43.
HPAHPA023389.
HPA027762.
neXtProtNX_Q70EL4.
PharmGKBPA134865304.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5560.
HOGENOMHOG000293426.
HOVERGENHBG094126.
InParanoidQ70EL4.
KOK11856.
OMAWQQQQAH.
OrthoDBEOG7VMP4B.
PhylomeDBQ70EL4.
TreeFamTF106278.

Gene expression databases

ArrayExpressQ70EL4.
BgeeQ70EL4.
CleanExHS_USP43.
GenevestigatorQ70EL4.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi124739.
NextBio81362.
PROQ70EL4.

Entry information

Entry nameUBP43_HUMAN
AccessionPrimary (citable) accession number: Q70EL4
Secondary accession number(s): A6NDT9 expand/collapse secondary AC list , B7ZLT9, B7ZVX5, Q8N2C5, Q96DQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM