Q70EL4 (UBP43_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 43
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 43
Ubiquitin thioesterase 43
Ubiquitin-specific-processing protease 43

Gene names

Name:

USP43

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1123 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Tissue specificity	Expressed in brain, aorta and lung at low levels. Ref.1
Sequence similarities	Belongs to the peptidase C19 family.
Sequence caution	The sequence BAB70869.1 differs from that shown. Reason: Frameshift at position 619.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q70EL4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q70EL4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-488: Missing.
     489-553: HLRRPGGPPH...ECFQFYTKEE → MPTVCGSSSR...GASQSGWFAF

Note: No experimental confirmation available.

Isoform 3 (identifier: Q70EL4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-311: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1123	1123	Ubiquitin carboxyl-terminal hydrolase 43		PRO_0000249521
Sites
	Active site	110	1	Nucleophile By similarity
	Active site	668	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	1041	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	1 – 488	488	Missing in isoform 2.		VSP_020466
	Alternative sequence	1 – 311	311	Missing in isoform 3.		VSP_020467
	Alternative sequence	489 – 553	65	HLRRP…YTKEE → MPTVCGSSSRRISSTAVPWM NVFSSTPRRSRSRPGGPCPG REGGWLPLVGWPRAAGASQS GWFAF in isoform 2.		VSP_020468
Experimental info
	Sequence conflict	168	1	K → FQ in CAE47744. Ref.1
	Sequence conflict	212	1	K → Q in CAE47744. Ref.1
	Sequence conflict	976	1	G → E in BAB70869. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: 3B4FE86EC619173C	FASTA	1,123	122,809
	10 20 30 40 50 60 MDLGPGDAAG GGPLAPRPRR RRSLRRLFSR FLLALGSRSR PGDSPPRPQP GHCDGDGEGG 70 80 90 100 110 120 FACAPGPVPA APGSPGEERP PGPQPQLQLP AGDGARPPGA QGLKNHGNTC FMNAVVQCLS 130 140 150 160 170 180 NTDLLAEFLA LGRYRAAPGR AEVTEQLAAL VRALWTREYT PQLSAEFKNA VSKYGSQFQG 190 200 210 220 230 240 NSQHDALEFL LWLLDRVHED LEGSSRGPVS EKLPPEATKT SENCLSPSAQ LPLGQSFVQS 250 260 270 280 290 300 HFQAQYRSSL TCPHCLKQSN TFDPFLCVSL PIPLRQTRFL SVTLVFPSKS QRFLRVGLAV 310 320 330 340 350 360 PILSTVAALR KMVAEEGGVP ADEVILVELY PSGFQRSFFD EEDLNTIAEG DNVYAFQVPP 370 380 390 400 410 420 SPSQGTLSAH PLGLSASPRL AAREGQRFSL SLHSESKVLI LFCNLVGSGQ QASRFGPPFL 430 440 450 460 470 480 IREDRAVSWA QLQQSILSKV RHLMKSEAPV QNLGSLFSIR VVGLSVACSY LSPKDSRPLC 490 500 510 520 530 540 HWAVDRVLHL RRPGGPPHVK LAVEWDSSVK ERLFGSLQEE RAQDADSVWQ QQQAHQQHSC 550 560 570 580 590 600 TLDECFQFYT KEEQLAQDDA WKCPHCQVLQ QGMVKLSLWT LPDILIIHLK RFCQVGERRN 610 620 630 640 650 660 KLSTLVKFPL SGLNMAPHVA QRSTSPEAGL GPWPSWKQPD CLPTSYPLDF LYDLYAVCNH 670 680 690 700 710 720 HGNLQGGHYT AYCRNSLDGQ WYSYDDSTVE PLREDEVNTR GAYILFYQKR NSIPPWSASS 730 740 750 760 770 780 SMRGSTSSSL SDHWLLRLGS HAGSTRGSLL SWSSAPCPSL PQVPDSPIFT NSLCNQEKGG 790 800 810 820 830 840 LEPRRLVRGV KGRSISMKAP TTSRAKQGPF KTMPLRWSFG SKEKPPGASV ELVEYLESRR 850 860 870 880 890 900 RPRSTSQSIV SLLTGTAGED EKSASPRSNV ALPANSEDGG RAIERGPAGV PCPSAQPNHC 910 920 930 940 950 960 LAPGNSDGPN TARKLKENAG QDIKLPRKFD LPLTVMPSVE HEKPARPEGQ KAMNWKESFQ 970 980 990 1000 1010 1020 MGSKSSPPSP YMGFSGNSKD SRRGTSELDR PLQGTLTLLR SVFRKKENRR NERAEVSPQV 1030 1040 1050 1060 1070 1080 PPVSLVSGGL SPAMDGQAPG SPPALRIPEG LARGLGSRLE RDVWSAPSSL RLPRKASRAP 1090 1100 1110 1120 RGSALGMSQR TVPGEQASYG TFQRVKYHTL SLGRKKTLPE SSF
	Isoform 2 [UniParc]. Checksum: EF610C195CD3F51D	FASTA	635	69,345
	10 20 30 40 50 60 MPTVCGSSSR RISSTAVPWM NVFSSTPRRS RSRPGGPCPG REGGWLPLVG WPRAAGASQS 70 80 90 100 110 120 GWFAFQLAQD DAWKCPHCQV LQQGMVKLSL WTLPDILIIH LKRFCQVGER RNKLSTLVKF 130 140 150 160 170 180 PLSGLNMAPH VAQRSTSPEA GLGPWPSWKQ PDCLPTSYPL DFLYDLYAVC NHHGNLQGGH 190 200 210 220 230 240 YTAYCRNSLD GQWYSYDDST VEPLREDEVN TRGAYILFYQ KRNSIPPWSA SSSMRGSTSS 250 260 270 280 290 300 SLSDHWLLRL GSHAGSTRGS LLSWSSAPCP SLPQVPDSPI FTNSLCNQEK GGLEPRRLVR 310 320 330 340 350 360 GVKGRSISMK APTTSRAKQG PFKTMPLRWS FGSKEKPPGA SVELVEYLES RRRPRSTSQS 370 380 390 400 410 420 IVSLLTGTAG EDEKSASPRS NVALPANSED GGRAIERGPA GVPCPSAQPN HCLAPGNSDG 430 440 450 460 470 480 PNTARKLKEN AGQDIKLPRK FDLPLTVMPS VEHEKPARPE GQKAMNWKES FQMGSKSSPP 490 500 510 520 530 540 SPYMGFSGNS KDSRRGTSEL DRPLQGTLTL LRSVFRKKEN RRNERAEVSP QVPPVSLVSG 550 560 570 580 590 600 GLSPAMDGQA PGSPPALRIP EGLARGLGSR LERDVWSAPS SLRLPRKASR APRGSALGMS 610 620 630 QRTVPGEQAS YGTFQRVKYH TLSLGRKKTL PESSF
	Isoform 3 [UniParc]. Checksum: 9E49012662FF0655	FASTA	812	89,337
	10 20 30 40 50 60 MVAEEGGVPA DEVILVELYP SGFQRSFFDE EDLNTIAEGD NVYAFQVPPS PSQGTLSAHP 70 80 90 100 110 120 LGLSASPRLA AREGQRFSLS LHSESKVLIL FCNLVGSGQQ ASRFGPPFLI REDRAVSWAQ 130 140 150 160 170 180 LQQSILSKVR HLMKSEAPVQ NLGSLFSIRV VGLSVACSYL SPKDSRPLCH WAVDRVLHLR 190 200 210 220 230 240 RPGGPPHVKL AVEWDSSVKE RLFGSLQEER AQDADSVWQQ QQAHQQHSCT LDECFQFYTK 250 260 270 280 290 300 EEQLAQDDAW KCPHCQVLQQ GMVKLSLWTL PDILIIHLKR FCQVGERRNK LSTLVKFPLS 310 320 330 340 350 360 GLNMAPHVAQ RSTSPEAGLG PWPSWKQPDC LPTSYPLDFL YDLYAVCNHH GNLQGGHYTA 370 380 390 400 410 420 YCRNSLDGQW YSYDDSTVEP LREDEVNTRG AYILFYQKRN SIPPWSASSS MRGSTSSSLS 430 440 450 460 470 480 DHWLLRLGSH AGSTRGSLLS WSSAPCPSLP QVPDSPIFTN SLCNQEKGGL EPRRLVRGVK 490 500 510 520 530 540 GRSISMKAPT TSRAKQGPFK TMPLRWSFGS KEKPPGASVE LVEYLESRRR PRSTSQSIVS 550 560 570 580 590 600 LLTGTAGEDE KSASPRSNVA LPANSEDGGR AIERGPAGVP CPSAQPNHCL APGNSDGPNT 610 620 630 640 650 660 ARKLKENAGQ DIKLPRKFDL PLTVMPSVEH EKPARPEGQK AMNWKESFQM GSKSSPPSPY 670 680 690 700 710 720 MGFSGNSKDS RRGTSELDRP LQGTLTLLRS VFRKKENRRN ERAEVSPQVP PVSLVSGGLS 730 740 750 760 770 780 PAMDGQAPGS PPALRIPEGL ARGLGSRLER DVWSAPSSLR LPRKASRAPR GSALGMSQRT 790 800 810 VPGEQASYGT FQRVKYHTLS LGRKKTLPES SF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases." Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S., Lopez-Otin C. Biochem. Biophys. Res. Commun. 314:54-62(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Amygdala.
[3]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1041, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ583817 mRNA. Translation: CAE47744.2. AK055188 mRNA. Translation: BAB70869.1. Frameshift. AK090821 mRNA. No translation available. AC027045 Genomic DNA. No translation available. AC118755 Genomic DNA. No translation available. CH471108 Genomic DNA. Translation: EAW90022.1. BC144041 mRNA. Translation: AAI44042.1.
IPI	IPI00166377. IPI00176488. IPI00739286.
RefSeq	NP_001254505.1. NM_001267576.1. NP_694942.3. NM_153210.4.
UniGene	Hs.709621.
3D structure databases
HSSP	HSSP built from PDB template 2AYN based on UniProtKB P54578.
ProteinModelPortal	Q70EL4.
SMR	Q70EL4. Positions 99-273, 539-711.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q70EL4. 9 interactions.
MINT	MINT-3976419.
STRING	9606.ENSP00000285199.
Protein family/group databases
MEROPS	C19.976.
PTM databases
PhosphoSite	Q70EL4.
Polymorphism databases
DMDM	296452852.
Proteomic databases
PaxDb	Q70EL4.
PRIDE	Q70EL4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000285199; ENSP00000285199; ENSG00000154914. ENST00000570827; ENSP00000461467; ENSG00000154914.
GeneID	124739.
KEGG	hsa:124739.
UCSC	uc002gma.4. human. uc002gmc.4. human.
Organism-specific databases
CTD	124739.
GeneCards	GC17P009489.
H-InvDB	HIX0013531.
HGNC	HGNC:20072. USP43.
HPA	HPA023389. HPA027762.
neXtProt	NX_Q70EL4.
PharmGKB	PA134865304.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5560.
HOGENOM	HOG000293426.
HOVERGEN	HBG094126.
InParanoid	Q70EL4.
KO	K11856.
OMA	RSISMKA.
OrthoDB	EOG4DR9BH.
Gene expression databases
ArrayExpress	Q70EL4.
Bgee	Q70EL4.
CleanEx	HS_USP43.
Genevestigator	Q70EL4.
GermOnline	ENSG00000154914. Homo sapiens.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	124739.
NextBio	81362.

Entry information

Entry name

UBP43_HUMAN

Accession

Primary (citable) accession number: Q70EL4
Secondary accession number(s): A6NDT9 , B7ZLT9, Q8N2C5, Q96DQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.