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Q70EL2

- UBP45_HUMAN

UniProt

Q70EL2 - UBP45_HUMAN

Protein

Ubiquitin carboxyl-terminal hydrolase 45

Gene

USP45

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 3 (18 May 2010)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei199 – 1991NucleophilePROSITE-ProRule annotation
    Active sitei746 – 7461Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri60 – 13677UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: FlyBase
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. protein deubiquitination Source: FlyBase
    2. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC19.064.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 45 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 45
    Ubiquitin thioesterase 45
    Ubiquitin-specific-processing protease 45
    Gene namesi
    Name:USP45
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:20080. USP45.

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134889604.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 814814Ubiquitin carboxyl-terminal hydrolase 45PRO_0000280561Add
    BLAST

    Proteomic databases

    MaxQBiQ70EL2.
    PaxDbiQ70EL2.
    PRIDEiQ70EL2.

    PTM databases

    PhosphoSiteiQ70EL2.

    Expressioni

    Tissue specificityi

    Broadly expressed, with highest levels in ovary, skeletal muscle and spleen.1 Publication

    Gene expression databases

    ArrayExpressiQ70EL2.
    BgeeiQ70EL2.
    CleanExiHS_USP45.
    GenevestigatoriQ70EL2.

    Organism-specific databases

    HPAiHPA029602.
    HPA029604.

    Interactioni

    Protein-protein interaction databases

    BioGridi124430. 22 interactions.
    IntActiQ70EL2. 21 interactions.
    STRINGi9606.ENSP00000333376.

    Structurei

    3D structure databases

    ProteinModelPortaliQ70EL2.
    SMRiQ70EL2. Positions 38-388, 603-810.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini190 – 813624USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri60 – 13677UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5207.
    HOGENOMiHOG000154755.
    HOVERGENiHBG062704.
    InParanoidiQ70EL2.
    KOiK11844.
    OMAiRIMKLCE.
    OrthoDBiEOG7J9VNZ.
    PhylomeDBiQ70EL2.
    TreeFamiTF326075.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q70EL2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRVKDPTKAL PEKAKRSKRP TVPHDEDSSD DIAVGLTCQH VSHAISVNHV    50
    KRAIAENLWS VCSECLKERR FYDGQLVLTS DIWLCLKCGF QGCGKNSESQ 100
    HSLKHFKSSR TEPHCIIINL STWIIWCYEC DEKLSTHCNK KVLAQIVDFL 150
    QKHASKTQTS AFSRIMKLCE EKCETDEIQK GGKCRNLSVR GITNLGNTCF 200
    FNAVMQNLAQ TYTLTDLMNE IKESSTKLKI FPSSDSQLDP LVVELSRPGP 250
    LTSALFLFLH SMKETEKGPL SPKVLFNQLC QKAPRFKDFQ QQDSQELLHY 300
    LLDAVRTEET KRIQASILKA FNNPTTKTAD DETRKKVKAY GKEGVKMNFI 350
    DRIFIGELTS TVMCEECANI STVKDPFIDI SLPIIEERVS KPLLWGRMNK 400
    YRSLRETDHD RYSGNVTIEN IHQPRAAKKH SSSKDKSQLI HDRKCIRKLS 450
    SGETVTYQKN ENLEMNGDSL MFASLMNSES RLNESPTDDS EKEASHSESN 500
    VDADSEPSES ESASKQTGLF RSSSGSGVQP DGPLYPLSAG KLLYTKETDS 550
    GDKEMAEAIS ELRLSSTVTG DQDFDRENQP LNISNNLCFL EGKHLRSYSP 600
    QNAFQTLSQS YITTSKECSI QSCLYQFTSM ELLMGNNKLL CENCTKNKQK 650
    YQEETSFAEK KVEGVYTNAR KQLLISAVPA VLILHLKRFH QAGLSLRKVN 700
    RHVDFPLMLD LAPFCSATCK NASVGDKVLY GLYGIVEHSG SMREGHYTAY 750
    VKVRTPSRKL SEHNTKKKNV PGLKAADNES AGQWVHVSDT YLQVVPESRA 800
    LSAQAYLLFY ERVL 814
    Length:814
    Mass (Da):91,733
    Last modified:May 18, 2010 - v3
    Checksum:iDE4FCC0AD7AD6499
    GO
    Isoform 2 (identifier: Q70EL2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         283-289: APRFKDF → RVHLHLI
         290-814: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:289
    Mass (Da):32,735
    Checksum:iB961DAA98F82939B
    GO
    Isoform 3 (identifier: Q70EL2-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-262: Missing.
         263-282: KETEKGPLSPKVLFNQLCQK → MRSKKVVQNSRFFLPQTLSW
         312-369: Missing.

    Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

    Show »
    Length:494
    Mass (Da):55,812
    Checksum:iE66E1435C4CAFA37
    GO

    Sequence cautioni

    The sequence CAI17192.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAI19761.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti603 – 6031A → V in CAD91148. (PubMed:17974005)Curated
    Sequence conflicti691 – 6911Q → K in CAE47746. (PubMed:14715245)Curated
    Sequence conflicti726 – 7261D → G in CAD89915. (PubMed:17974005)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti67 – 671K → E.1 Publication
    Corresponds to variant rs7744845 [ dbSNP | Ensembl ].
    VAR_031167
    Natural varianti521 – 5211R → T.1 Publication
    Corresponds to variant rs41288947 [ dbSNP | Ensembl ].
    VAR_060663
    Natural varianti778 – 7781N → S.2 Publications
    Corresponds to variant rs6570065 [ dbSNP | Ensembl ].
    VAR_031168

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 262262Missing in isoform 3. 1 PublicationVSP_023789Add
    BLAST
    Alternative sequencei263 – 28220KETEK…QLCQK → MRSKKVVQNSRFFLPQTLSW in isoform 3. 1 PublicationVSP_023790Add
    BLAST
    Alternative sequencei283 – 2897APRFKDF → RVHLHLI in isoform 2. 1 PublicationVSP_023791
    Alternative sequencei290 – 814525Missing in isoform 2. 1 PublicationVSP_023792Add
    BLAST
    Alternative sequencei312 – 36958Missing in isoform 3. 1 PublicationVSP_023793Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ583819 mRNA. Translation: CAE47746.2.
    AL713747 mRNA. Translation: CAD89915.1.
    AL832030 mRNA. Translation: CAD91148.1.
    AL513550, AL137784 Genomic DNA. Translation: CAI17192.1. Sequence problems.
    AL137784, AL513550 Genomic DNA. Translation: CAI19761.1. Sequence problems.
    BC005991 mRNA. Translation: AAH05991.1.
    BC150648 mRNA. Translation: AAI50649.1.
    BC157838 mRNA. Translation: AAI57839.1.
    CCDSiCCDS34501.1. [Q70EL2-1]
    RefSeqiNP_001073950.1. NM_001080481.1. [Q70EL2-1]
    XP_005267226.1. XM_005267169.1. [Q70EL2-1]
    XP_005267227.1. XM_005267170.2. [Q70EL2-1]
    UniGeneiHs.143410.

    Genome annotation databases

    EnsembliENST00000327681; ENSP00000333376; ENSG00000123552. [Q70EL2-1]
    ENST00000500704; ENSP00000424372; ENSG00000123552. [Q70EL2-1]
    GeneIDi85015.
    KEGGihsa:85015.
    UCSCiuc003ppw.2. human. [Q70EL2-3]
    uc003ppx.2. human. [Q70EL2-1]

    Polymorphism databases

    DMDMi296453002.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ583819 mRNA. Translation: CAE47746.2 .
    AL713747 mRNA. Translation: CAD89915.1 .
    AL832030 mRNA. Translation: CAD91148.1 .
    AL513550 , AL137784 Genomic DNA. Translation: CAI17192.1 . Sequence problems.
    AL137784 , AL513550 Genomic DNA. Translation: CAI19761.1 . Sequence problems.
    BC005991 mRNA. Translation: AAH05991.1 .
    BC150648 mRNA. Translation: AAI50649.1 .
    BC157838 mRNA. Translation: AAI57839.1 .
    CCDSi CCDS34501.1. [Q70EL2-1 ]
    RefSeqi NP_001073950.1. NM_001080481.1. [Q70EL2-1 ]
    XP_005267226.1. XM_005267169.1. [Q70EL2-1 ]
    XP_005267227.1. XM_005267170.2. [Q70EL2-1 ]
    UniGenei Hs.143410.

    3D structure databases

    ProteinModelPortali Q70EL2.
    SMRi Q70EL2. Positions 38-388, 603-810.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 124430. 22 interactions.
    IntActi Q70EL2. 21 interactions.
    STRINGi 9606.ENSP00000333376.

    Protein family/group databases

    MEROPSi C19.064.

    PTM databases

    PhosphoSitei Q70EL2.

    Polymorphism databases

    DMDMi 296453002.

    Proteomic databases

    MaxQBi Q70EL2.
    PaxDbi Q70EL2.
    PRIDEi Q70EL2.

    Protocols and materials databases

    DNASUi 85015.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000327681 ; ENSP00000333376 ; ENSG00000123552 . [Q70EL2-1 ]
    ENST00000500704 ; ENSP00000424372 ; ENSG00000123552 . [Q70EL2-1 ]
    GeneIDi 85015.
    KEGGi hsa:85015.
    UCSCi uc003ppw.2. human. [Q70EL2-3 ]
    uc003ppx.2. human. [Q70EL2-1 ]

    Organism-specific databases

    CTDi 85015.
    GeneCardsi GC06M099925.
    H-InvDB HIX0006090.
    HGNCi HGNC:20080. USP45.
    HPAi HPA029602.
    HPA029604.
    neXtProti NX_Q70EL2.
    PharmGKBi PA134889604.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5207.
    HOGENOMi HOG000154755.
    HOVERGENi HBG062704.
    InParanoidi Q70EL2.
    KOi K11844.
    OMAi RIMKLCE.
    OrthoDBi EOG7J9VNZ.
    PhylomeDBi Q70EL2.
    TreeFami TF326075.

    Miscellaneous databases

    ChiTaRSi USP45. human.
    GenomeRNAii 85015.
    NextBioi 75631.
    PROi Q70EL2.

    Gene expression databases

    ArrayExpressi Q70EL2.
    Bgeei Q70EL2.
    CleanExi HS_USP45.
    Genevestigatori Q70EL2.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases."
      Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S., Lopez-Otin C.
      Biochem. Biophys. Res. Commun. 314:54-62(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 248-814 (ISOFORM 1), VARIANT SER-778.
      Tissue: Skeletal muscle.
    3. "The DNA sequence and analysis of human chromosome 6."
      Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
      Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANTS GLU-67; THR-521 AND SER-778.
      Tissue: Bone marrow and Brain cortex.

    Entry informationi

    Entry nameiUBP45_HUMAN
    AccessioniPrimary (citable) accession number: Q70EL2
    Secondary accession number(s): B2RXG0
    , Q5T062, Q86T44, Q86TC0, Q9BRU1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 2007
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 90 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. Peptidase families
      Classification of peptidase families and list of entries
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3