Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q70EL2 (UBP45_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 45

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 45
Ubiquitin thioesterase 45
Ubiquitin-specific-processing protease 45
Gene names
Name:USP45
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length814 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). Ref.1

Tissue specificity

Broadly expressed, with highest levels in ovary, skeletal muscle and spleen. Ref.1

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Sequence caution

The sequence CAI17192.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAI19761.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q70EL2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q70EL2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     283-289: APRFKDF → RVHLHLI
     290-814: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q70EL2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-262: Missing.
     263-282: KETEKGPLSPKVLFNQLCQK → MRSKKVVQNSRFFLPQTLSW
     312-369: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 814814Ubiquitin carboxyl-terminal hydrolase 45
PRO_0000280561

Regions

Domain190 – 813624USP
Zinc finger60 – 13677UBP-type

Sites

Active site1991Nucleophile By similarity
Active site7461Proton acceptor By similarity

Natural variations

Alternative sequence1 – 262262Missing in isoform 3.
VSP_023789
Alternative sequence263 – 28220KETEK…QLCQK → MRSKKVVQNSRFFLPQTLSW in isoform 3.
VSP_023790
Alternative sequence283 – 2897APRFKDF → RVHLHLI in isoform 2.
VSP_023791
Alternative sequence290 – 814525Missing in isoform 2.
VSP_023792
Alternative sequence312 – 36958Missing in isoform 3.
VSP_023793
Natural variant671K → E. Ref.4
Corresponds to variant rs7744845 [ dbSNP | Ensembl ].
VAR_031167
Natural variant5211R → T. Ref.4
Corresponds to variant rs41288947 [ dbSNP | Ensembl ].
VAR_060663
Natural variant7781N → S. Ref.2 Ref.4
Corresponds to variant rs6570065 [ dbSNP | Ensembl ].
VAR_031168

Experimental info

Sequence conflict6031A → V in CAD91148. Ref.2
Sequence conflict6911Q → K in CAE47746. Ref.1
Sequence conflict7261D → G in CAD89915. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 18, 2010. Version 3.
Checksum: DE4FCC0AD7AD6499

FASTA81491,733
        10         20         30         40         50         60 
MRVKDPTKAL PEKAKRSKRP TVPHDEDSSD DIAVGLTCQH VSHAISVNHV KRAIAENLWS 

        70         80         90        100        110        120 
VCSECLKERR FYDGQLVLTS DIWLCLKCGF QGCGKNSESQ HSLKHFKSSR TEPHCIIINL 

       130        140        150        160        170        180 
STWIIWCYEC DEKLSTHCNK KVLAQIVDFL QKHASKTQTS AFSRIMKLCE EKCETDEIQK 

       190        200        210        220        230        240 
GGKCRNLSVR GITNLGNTCF FNAVMQNLAQ TYTLTDLMNE IKESSTKLKI FPSSDSQLDP 

       250        260        270        280        290        300 
LVVELSRPGP LTSALFLFLH SMKETEKGPL SPKVLFNQLC QKAPRFKDFQ QQDSQELLHY 

       310        320        330        340        350        360 
LLDAVRTEET KRIQASILKA FNNPTTKTAD DETRKKVKAY GKEGVKMNFI DRIFIGELTS 

       370        380        390        400        410        420 
TVMCEECANI STVKDPFIDI SLPIIEERVS KPLLWGRMNK YRSLRETDHD RYSGNVTIEN 

       430        440        450        460        470        480 
IHQPRAAKKH SSSKDKSQLI HDRKCIRKLS SGETVTYQKN ENLEMNGDSL MFASLMNSES 

       490        500        510        520        530        540 
RLNESPTDDS EKEASHSESN VDADSEPSES ESASKQTGLF RSSSGSGVQP DGPLYPLSAG 

       550        560        570        580        590        600 
KLLYTKETDS GDKEMAEAIS ELRLSSTVTG DQDFDRENQP LNISNNLCFL EGKHLRSYSP 

       610        620        630        640        650        660 
QNAFQTLSQS YITTSKECSI QSCLYQFTSM ELLMGNNKLL CENCTKNKQK YQEETSFAEK 

       670        680        690        700        710        720 
KVEGVYTNAR KQLLISAVPA VLILHLKRFH QAGLSLRKVN RHVDFPLMLD LAPFCSATCK 

       730        740        750        760        770        780 
NASVGDKVLY GLYGIVEHSG SMREGHYTAY VKVRTPSRKL SEHNTKKKNV PGLKAADNES 

       790        800        810 
AGQWVHVSDT YLQVVPESRA LSAQAYLLFY ERVL 

« Hide

Isoform 2 [UniParc].

Checksum: B961DAA98F82939B
Show »

FASTA28932,735
Isoform 3 [UniParc].

Checksum: E66E1435C4CAFA37
Show »

FASTA49455,812

References

« Hide 'large scale' references
[1]"Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases."
Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S., Lopez-Otin C.
Biochem. Biophys. Res. Commun. 314:54-62(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 248-814 (ISOFORM 1), VARIANT SER-778.
Tissue: Skeletal muscle.
[3]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANTS GLU-67; THR-521 AND SER-778.
Tissue: Bone marrow and Brain cortex.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ583819 mRNA. Translation: CAE47746.2.
AL713747 mRNA. Translation: CAD89915.1.
AL832030 mRNA. Translation: CAD91148.1.
AL513550, AL137784 Genomic DNA. Translation: CAI17192.1. Sequence problems.
AL137784, AL513550 Genomic DNA. Translation: CAI19761.1. Sequence problems.
BC005991 mRNA. Translation: AAH05991.1.
BC150648 mRNA. Translation: AAI50649.1.
BC157838 mRNA. Translation: AAI57839.1.
RefSeqNP_001073950.1. NM_001080481.1.
XP_005267226.1. XM_005267169.1.
XP_005267227.1. XM_005267170.2.
UniGeneHs.143410.

3D structure databases

ProteinModelPortalQ70EL2.
SMRQ70EL2. Positions 38-388, 603-810.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid124430. 22 interactions.
IntActQ70EL2. 21 interactions.
STRING9606.ENSP00000333376.

Protein family/group databases

MEROPSC19.064.

PTM databases

PhosphoSiteQ70EL2.

Polymorphism databases

DMDM296453002.

Proteomic databases

PaxDbQ70EL2.
PRIDEQ70EL2.

Protocols and materials databases

DNASU85015.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000327681; ENSP00000333376; ENSG00000123552. [Q70EL2-1]
ENST00000392738; ENSP00000376495; ENSG00000123552. [Q70EL2-3]
ENST00000500704; ENSP00000424372; ENSG00000123552. [Q70EL2-1]
GeneID85015.
KEGGhsa:85015.
UCSCuc003ppw.2. human. [Q70EL2-3]
uc003ppx.2. human. [Q70EL2-1]

Organism-specific databases

CTD85015.
GeneCardsGC06M099925.
H-InvDBHIX0006090.
HGNCHGNC:20080. USP45.
HPAHPA029602.
HPA029604.
neXtProtNX_Q70EL2.
PharmGKBPA134889604.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5207.
HOGENOMHOG000154755.
HOVERGENHBG062704.
InParanoidQ70EL2.
KOK11844.
OMANNLCFSE.
OrthoDBEOG7J9VNZ.
PhylomeDBQ70EL2.
TreeFamTF326075.

Gene expression databases

ArrayExpressQ70EL2.
BgeeQ70EL2.
CleanExHS_USP45.
GenevestigatorQ70EL2.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSUSP45. human.
GenomeRNAi85015.
NextBio75631.
PROQ70EL2.

Entry information

Entry nameUBP45_HUMAN
AccessionPrimary (citable) accession number: Q70EL2
Secondary accession number(s): B2RXG0 expand/collapse secondary AC list , Q5T062, Q86T44, Q86TC0, Q9BRU1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: May 18, 2010
Last modified: April 16, 2014
This is version 87 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM