Q70D51 (SIAT9_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lactosylceramide alpha-2,3-sialyltransferase
EC=2.4.99.9

Alternative name(s):
CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase
Ganglioside GM3 synthase
ST3Gal V
Short name=ST3GalV
Sialyltransferase 9

Gene names

Name:	ST3GAL5
Synonyms:	SIAT9

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of ganglioside GM3 (alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1, 4-beta-D-glucosylceramide) By similarity.
Catalytic activity	CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 29 family.
Sequence caution	The sequence CAE51392.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular function	lactosylceramide alpha-2,3-sialyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Lactosylceramide alpha-2,3-sialyltransferase		PRO_0000334617
Regions
	Topological domain	1 – 67	67	Cytoplasmic Potential
	Transmembrane	68 – 88	21	Helical; Potential
	Topological domain	89 – 420	332	Lumenal Potential
	Compositional bias	20 – 26	7	Poly-Ala
Amino acid modifications
	Glycosylation	238	1	N-linked (GlcNAc...) Potential
	Disulfide bond	197 ↔ 355		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q70D51 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: B8E51833DB069032	FASTA	420	48,263
	10 20 30 40 50 60 MRKKAAGGAE RRPLKPRTEA AAAAPAGRAM PSDHSRMKLR RDCSRPSLQW YTRAQNKMRR 70 80 90 100 110 120 PNLLLKDILK CTLLLFGVWI LFYILKLNHT TEECDMKRMP YMDPDRIKRA QQYAQQVLQK 130 140 150 160 170 180 ECRPQFAKRS MAQLFGSRYS LDLPPFVTKV PAESEAEYKY DPPFGFRKFS GKVQTLLELL 190 200 210 220 230 240 PEHDFPEHLR AKSCKHCVVI GSGGILHGLE MGHALNQFDV VIRLNNAPVE GYSEHVGNKT 250 260 270 280 290 300 TIRMTYPEGA PLSDLEYYSS DLFVTVLFKS VDFNWLQAMV KNETLPFWVR LFFWKQVAEK 310 320 330 340 350 360 IPLQPKQFRI LNPVIIKETA FDILQYSEPQ SRFWGRDKNV PTIGVIAVVL ATHLCDEVSL 370 380 390 400 410 420 AGFSYALNQP RTPLHYFDNL CMAAMNFQTM HNVTTETNFL LKLVREGVVR DLSGGIHSEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of taurine cattle: a window to ruminant biology and evolution." The bovine genome sequencing and analysis consortium Science 324:522-528(2009) [PubMed: 19390049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hereford.
[2]	"The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach." Harduin-Lepers A., Mollicone R., Delannoy P., Oriol R. Glycobiology 15:805-817(2005) [PubMed: 15843597] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 20-420.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFC03040697 Genomic DNA. No translation available. AAFC03065753 Genomic DNA. No translation available. AJ585768 mRNA. Translation: CAE51392.1. Different initiation.
IPI	IPI00688424.
RefSeq	NP_991376.2. NM_205807.2.
UniGene	Bt.52500.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q70D51.
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
Proteomic databases
PRIDE	Q70D51.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	404164.
KEGG	bta:404164.
Organism-specific databases
CTD	8869.
Phylogenomic databases
eggNOG	maNOG04027.
GeneTree	ENSGT00550000074199.
HOVERGEN	HBG056676.
InParanoid	Q70D51.
OrthoDB	EOG47PX6C.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. IPR012163. Sialyl_trans. [Graphical view]
KO	K03370.
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
PIRSF	PIRSF005557. Sialyl_trans. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SIAT9_BOVIN

Accession

Primary (citable) accession number: Q70D51

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	November 16, 2011
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program