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Protein

Ubiquitin carboxyl-terminal hydrolase 31

Gene

USP31

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei137NucleophilePROSITE-ProRule annotation1
Active sitei723Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • thiol-dependent ubiquitin-specific protease activity Source: FlyBase

GO - Biological processi

  • protein deubiquitination Source: FlyBase
  • ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:HS02499-MONOMER.

Protein family/group databases

MEROPSiC19.071.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 31 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 31
Ubiquitin thioesterase 31
Ubiquitin-specific-processing protease 31
Gene namesi
Name:USP31
Synonyms:KIAA1203
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:20060. USP31.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: FlyBase
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000103404.
PharmGKBiPA164742780.

Polymorphism and mutation databases

BioMutaiUSP31.
DMDMi134047944.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002495181 – 1352Ubiquitin carboxyl-terminal hydrolase 31Add BLAST1352

Proteomic databases

EPDiQ70CQ4.
MaxQBiQ70CQ4.
PaxDbiQ70CQ4.
PeptideAtlasiQ70CQ4.
PRIDEiQ70CQ4.
TopDownProteomicsiQ70CQ4-2. [Q70CQ4-2]

PTM databases

iPTMnetiQ70CQ4.
PhosphoSitePlusiQ70CQ4.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000103404.
CleanExiHS_USP31.
ExpressionAtlasiQ70CQ4. baseline and differential.
GenevisibleiQ70CQ4. HS.

Organism-specific databases

HPAiHPA006937.

Interactioni

Protein-protein interaction databases

BioGridi121548. 2 interactors.
STRINGi9606.ENSP00000219689.

Structurei

3D structure databases

ProteinModelPortaliQ70CQ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini128 – 765USPAdd BLAST638

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi95 – 125Pro-richAdd BLAST31
Compositional biasi770 – 1221Ser-richAdd BLAST452

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiKOG1870. Eukaryota.
COG5560. LUCA.
GeneTreeiENSGT00670000097750.
HOGENOMiHOG000154758.
HOVERGENiHBG094125.
InParanoidiQ70CQ4.
KOiK11852.
OrthoDBiEOG091G05I6.
PhylomeDBiQ70CQ4.
TreeFamiTF106278.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q70CQ4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKVTAPGSG PPAAASGKEK RSFSKRLFRS GRAGGGGAGG PGASGPAAPS
60 70 80 90 100
SPSSPSSARS VGSFMSRVLK TLSTLSHLSS EGAAPDRGGL RSCFPPGPAA
110 120 130 140 150
APTPPPCPPP PASPAPPACA AEPVPGVAGL RNHGNTCFMN ATLQCLSNTE
160 170 180 190 200
LFAEYLALGQ YRAGRPEPSP DPEQPAGRGA QGQGEVTEQL AHLVRALWTL
210 220 230 240 250
EYTPQHSRDF KTIVSKNALQ YRGNSQHDAQ EFLLWLLDRV HEDLNHSVKQ
260 270 280 290 300
SGQPPLKPPS ETDMMPEGPS FPVCSTFVQE LFQAQYRSSL TCPHCQKQSN
310 320 330 340 350
TFDPFLCISL PIPLPHTRPL YVTVVYQGKC SHCMRIGVAV PLSGTVARLR
360 370 380 390 400
EAVSMETKIP TDQIVLTEMY YDGFHRSFCD TDDLETVHES DCIFAFETPE
410 420 430 440 450
IFRPEGILSQ RGIHLNNNLN HLKFGLDYHR LSSPTQTAAK QGKMDSPTSR
460 470 480 490 500
AGSDKIVLLV CNRACTGQQG KRFGLPFVLH LEKTIAWDLL QKEILEKMKY
510 520 530 540 550
FLRPTVCIQV CPFSLRVVSV VGITYLLPQE EQPLCHPIVE RALKSCGPGG
560 570 580 590 600
TAHVKLVVEW DKETRDFLFV NTEDEYIPDA ESVRLQRERH HQPQTCTLSQ
610 620 630 640 650
CFQLYTKEER LAPDDAWRCP HCKQLQQGSI TLSLWTLPDV LIIHLKRFRQ
660 670 680 690 700
EGDRRMKLQN MVKFPLTGLD MTPHVVKRSQ SSWSLPSHWS PWRRPYGLGR
710 720 730 740 750
DPEDYIYDLY AVCNHHGTMQ GGHYTAYCKN SVDGLWYCFD DSDVQQLSED
760 770 780 790 800
EVCTQTAYIL FYQRRTAIPS WSANSSVAGS TSSSLCEHWV SRLPGSKPAS
810 820 830 840 850
VTSAASSRRT SLASLSESVE MTGERSEDDG GFSTRPFVRS VQRQSLSSRS
860 870 880 890 900
SVTSPLAVNE NCMRPSWSLS AKLQMRSNSP SRFSGDSPIH SSASTLEKIG
910 920 930 940 950
EAADDKVSIS CFGSLRNLSS SYQEPSDSHS RREHKAVGRA PLAVMEGVFK
960 970 980 990 1000
DESDTRRLNS SVVDTQSKHS AQGDRLPPLS GPFDNNNQIA YVDQSDSVDS
1010 1020 1030 1040 1050
SPVKEVKAPS HPGSLAKKPE STTKRSPSSK GTSEPEKSLR KGRPALASQE
1060 1070 1080 1090 1100
SSLSSTSPSS PLPVKVSLKP SRSRSKADSS SRGSGRHSSP APAQPKKESS
1110 1120 1130 1140 1150
PKSQDSVSSP SPQKQKSASA LTYTASSTSA KKASGPATRS PFPPGKSRTS
1160 1170 1180 1190 1200
DHSLSREGSR QSLGSDRASA TSTSKPNSPR VSQARAGEGR GAGKHVRSSS
1210 1220 1230 1240 1250
MASLRSPSTS IKSGLKRDSK SEDKGLSFFK SALRQKETRR STDLGKTALL
1260 1270 1280 1290 1300
SKKAGGSSVK SVCKNTGDDE AERGHQPPAS QQPNANTTGK EQLVTKDPAS
1310 1320 1330 1340 1350
AKHSLLSARK SKSSQLDSGV PSSPGGRQSA EKSSKKLSSS MQTSARPSQK

PQ
Length:1,352
Mass (Da):146,651
Last modified:March 20, 2007 - v2
Checksum:iF68A90EC45D23EDF
GO
Isoform 2 (identifier: Q70CQ4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-697: Missing.
     698-725: LGRDPEDYIYDLYAVCNHHGTMQGGHYT → MSQRDGVCGSHEVAGAASPGADGGLSLA
     1095-1140: PKKESSPKSQ...KKASGPATRS → TQFPSGEPGP...QVWFEEGQQV
     1141-1352: Missing.

Note: No experimental confirmation available.
Show »
Length:443
Mass (Da):47,129
Checksum:i00B4E309593EC2E9
GO

Sequence cautioni

The sequence AK126752 differs from that shown. Reason: Frameshift at position 941.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti568L → P in CAE51935 (PubMed:14715245).Curated1
Sequence conflicti624Q → H in AK126752 (PubMed:14702039).Curated1
Sequence conflicti1082R → S in AK126752 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027422445D → Y.Corresponds to variant rs1978066dbSNPEnsembl.1
Natural variantiVAR_027423532Q → H.Corresponds to variant rs4597335dbSNPEnsembl.1
Natural variantiVAR_027424538I → T.1 PublicationCorresponds to variant rs13339649dbSNPEnsembl.1
Natural variantiVAR_027425552A → T.Corresponds to variant rs9932912dbSNPEnsembl.1
Natural variantiVAR_027426931R → L.Corresponds to variant rs10083789dbSNPEnsembl.1
Natural variantiVAR_0515341269D → N.Corresponds to variant rs35541113dbSNPEnsembl.1
Natural variantiVAR_0515351309R → C.Corresponds to variant rs35254998dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0204591 – 697Missing in isoform 2. 1 PublicationAdd BLAST697
Alternative sequenceiVSP_020460698 – 725LGRDP…GGHYT → MSQRDGVCGSHEVAGAASPG ADGGLSLA in isoform 2. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_0204611095 – 1140PKKES…PATRS → TQFPSGEPGPSRGGQGGREA RAELLHGQPALPQHKHQVWF EEGQQV in isoform 2. 1 PublicationAdd BLAST46
Alternative sequenceiVSP_0204621141 – 1352Missing in isoform 2. 1 PublicationAdd BLAST212

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ586135 mRNA. Translation: CAE51935.2.
AK126447 mRNA. Translation: BAC86554.1.
AK126752 mRNA. No translation available.
AB033029 mRNA. Translation: BAA86517.1.
BX648357 mRNA. Translation: CAH10383.1.
CCDSiCCDS10607.1. [Q70CQ4-1]
RefSeqiNP_065769.3. NM_020718.3. [Q70CQ4-1]
UniGeneiHs.183817.

Genome annotation databases

EnsembliENST00000219689; ENSP00000219689; ENSG00000103404. [Q70CQ4-1]
GeneIDi57478.
KEGGihsa:57478.
UCSCiuc002dll.4. human. [Q70CQ4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ586135 mRNA. Translation: CAE51935.2.
AK126447 mRNA. Translation: BAC86554.1.
AK126752 mRNA. No translation available.
AB033029 mRNA. Translation: BAA86517.1.
BX648357 mRNA. Translation: CAH10383.1.
CCDSiCCDS10607.1. [Q70CQ4-1]
RefSeqiNP_065769.3. NM_020718.3. [Q70CQ4-1]
UniGeneiHs.183817.

3D structure databases

ProteinModelPortaliQ70CQ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121548. 2 interactors.
STRINGi9606.ENSP00000219689.

Protein family/group databases

MEROPSiC19.071.

PTM databases

iPTMnetiQ70CQ4.
PhosphoSitePlusiQ70CQ4.

Polymorphism and mutation databases

BioMutaiUSP31.
DMDMi134047944.

Proteomic databases

EPDiQ70CQ4.
MaxQBiQ70CQ4.
PaxDbiQ70CQ4.
PeptideAtlasiQ70CQ4.
PRIDEiQ70CQ4.
TopDownProteomicsiQ70CQ4-2. [Q70CQ4-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000219689; ENSP00000219689; ENSG00000103404. [Q70CQ4-1]
GeneIDi57478.
KEGGihsa:57478.
UCSCiuc002dll.4. human. [Q70CQ4-1]

Organism-specific databases

CTDi57478.
GeneCardsiUSP31.
H-InvDBHIX0012889.
HGNCiHGNC:20060. USP31.
HPAiHPA006937.
neXtProtiNX_Q70CQ4.
OpenTargetsiENSG00000103404.
PharmGKBiPA164742780.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1870. Eukaryota.
COG5560. LUCA.
GeneTreeiENSGT00670000097750.
HOGENOMiHOG000154758.
HOVERGENiHBG094125.
InParanoidiQ70CQ4.
KOiK11852.
OrthoDBiEOG091G05I6.
PhylomeDBiQ70CQ4.
TreeFamiTF106278.

Enzyme and pathway databases

BioCyciZFISH:HS02499-MONOMER.

Miscellaneous databases

ChiTaRSiUSP31. human.
GenomeRNAii57478.
PROiQ70CQ4.

Gene expression databases

BgeeiENSG00000103404.
CleanExiHS_USP31.
ExpressionAtlasiQ70CQ4. baseline and differential.
GenevisibleiQ70CQ4. HS.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP31_HUMAN
AccessioniPrimary (citable) accession number: Q70CQ4
Secondary accession number(s): Q6AW97
, Q6ZTC0, Q6ZTN2, Q9ULL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: March 20, 2007
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.