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Q70CQ4 (UBP31_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 31

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 31
Ubiquitin thioesterase 31
Ubiquitin-specific-processing protease 31
Gene names
Name:USP31
Synonyms:KIAA1203
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1352 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Tissue specificity

Widely expressed. Ref.1

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Sequence caution

The sequence AK126752 differs from that shown. Reason: Frameshift at position 941.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q70CQ4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q70CQ4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-697: Missing.
     698-725: LGRDPEDYIYDLYAVCNHHGTMQGGHYT → MSQRDGVCGSHEVAGAASPGADGGLSLA
     1095-1140: PKKESSPKSQ...KKASGPATRS → TQFPSGEPGP...QVWFEEGQQV
     1141-1352: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13521352Ubiquitin carboxyl-terminal hydrolase 31
PRO_0000249518

Regions

Domain128 – 765638USP
Compositional bias95 – 12531Pro-rich
Compositional bias770 – 1221452Ser-rich

Sites

Active site1371Nucleophile By similarity
Active site7231Proton acceptor By similarity

Natural variations

Alternative sequence1 – 697697Missing in isoform 2.
VSP_020459
Alternative sequence698 – 72528LGRDP…GGHYT → MSQRDGVCGSHEVAGAASPG ADGGLSLA in isoform 2.
VSP_020460
Alternative sequence1095 – 114046PKKES…PATRS → TQFPSGEPGPSRGGQGGREA RAELLHGQPALPQHKHQVWF EEGQQV in isoform 2.
VSP_020461
Alternative sequence1141 – 1352212Missing in isoform 2.
VSP_020462
Natural variant4451D → Y.
Corresponds to variant rs1978066 [ dbSNP | Ensembl ].
VAR_027422
Natural variant5321Q → H.
Corresponds to variant rs4597335 [ dbSNP | Ensembl ].
VAR_027423
Natural variant5381I → T. Ref.2
Corresponds to variant rs13339649 [ dbSNP | Ensembl ].
VAR_027424
Natural variant5521A → T.
Corresponds to variant rs9932912 [ dbSNP | Ensembl ].
VAR_027425
Natural variant9311R → L.
Corresponds to variant rs10083789 [ dbSNP | Ensembl ].
VAR_027426
Natural variant12691D → N.
Corresponds to variant rs35541113 [ dbSNP | Ensembl ].
VAR_051534
Natural variant13091R → C.
Corresponds to variant rs35254998 [ dbSNP | Ensembl ].
VAR_051535

Experimental info

Sequence conflict5681L → P in CAE51935. Ref.1
Sequence conflict6241Q → H in AK126752. Ref.2
Sequence conflict10821R → S in AK126752. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 20, 2007. Version 2.
Checksum: F68A90EC45D23EDF

FASTA1,352146,651
        10         20         30         40         50         60 
MSKVTAPGSG PPAAASGKEK RSFSKRLFRS GRAGGGGAGG PGASGPAAPS SPSSPSSARS 

        70         80         90        100        110        120 
VGSFMSRVLK TLSTLSHLSS EGAAPDRGGL RSCFPPGPAA APTPPPCPPP PASPAPPACA 

       130        140        150        160        170        180 
AEPVPGVAGL RNHGNTCFMN ATLQCLSNTE LFAEYLALGQ YRAGRPEPSP DPEQPAGRGA 

       190        200        210        220        230        240 
QGQGEVTEQL AHLVRALWTL EYTPQHSRDF KTIVSKNALQ YRGNSQHDAQ EFLLWLLDRV 

       250        260        270        280        290        300 
HEDLNHSVKQ SGQPPLKPPS ETDMMPEGPS FPVCSTFVQE LFQAQYRSSL TCPHCQKQSN 

       310        320        330        340        350        360 
TFDPFLCISL PIPLPHTRPL YVTVVYQGKC SHCMRIGVAV PLSGTVARLR EAVSMETKIP 

       370        380        390        400        410        420 
TDQIVLTEMY YDGFHRSFCD TDDLETVHES DCIFAFETPE IFRPEGILSQ RGIHLNNNLN 

       430        440        450        460        470        480 
HLKFGLDYHR LSSPTQTAAK QGKMDSPTSR AGSDKIVLLV CNRACTGQQG KRFGLPFVLH 

       490        500        510        520        530        540 
LEKTIAWDLL QKEILEKMKY FLRPTVCIQV CPFSLRVVSV VGITYLLPQE EQPLCHPIVE 

       550        560        570        580        590        600 
RALKSCGPGG TAHVKLVVEW DKETRDFLFV NTEDEYIPDA ESVRLQRERH HQPQTCTLSQ 

       610        620        630        640        650        660 
CFQLYTKEER LAPDDAWRCP HCKQLQQGSI TLSLWTLPDV LIIHLKRFRQ EGDRRMKLQN 

       670        680        690        700        710        720 
MVKFPLTGLD MTPHVVKRSQ SSWSLPSHWS PWRRPYGLGR DPEDYIYDLY AVCNHHGTMQ 

       730        740        750        760        770        780 
GGHYTAYCKN SVDGLWYCFD DSDVQQLSED EVCTQTAYIL FYQRRTAIPS WSANSSVAGS 

       790        800        810        820        830        840 
TSSSLCEHWV SRLPGSKPAS VTSAASSRRT SLASLSESVE MTGERSEDDG GFSTRPFVRS 

       850        860        870        880        890        900 
VQRQSLSSRS SVTSPLAVNE NCMRPSWSLS AKLQMRSNSP SRFSGDSPIH SSASTLEKIG 

       910        920        930        940        950        960 
EAADDKVSIS CFGSLRNLSS SYQEPSDSHS RREHKAVGRA PLAVMEGVFK DESDTRRLNS 

       970        980        990       1000       1010       1020 
SVVDTQSKHS AQGDRLPPLS GPFDNNNQIA YVDQSDSVDS SPVKEVKAPS HPGSLAKKPE 

      1030       1040       1050       1060       1070       1080 
STTKRSPSSK GTSEPEKSLR KGRPALASQE SSLSSTSPSS PLPVKVSLKP SRSRSKADSS 

      1090       1100       1110       1120       1130       1140 
SRGSGRHSSP APAQPKKESS PKSQDSVSSP SPQKQKSASA LTYTASSTSA KKASGPATRS 

      1150       1160       1170       1180       1190       1200 
PFPPGKSRTS DHSLSREGSR QSLGSDRASA TSTSKPNSPR VSQARAGEGR GAGKHVRSSS 

      1210       1220       1230       1240       1250       1260 
MASLRSPSTS IKSGLKRDSK SEDKGLSFFK SALRQKETRR STDLGKTALL SKKAGGSSVK 

      1270       1280       1290       1300       1310       1320 
SVCKNTGDDE AERGHQPPAS QQPNANTTGK EQLVTKDPAS AKHSLLSARK SKSSQLDSGV 

      1330       1340       1350 
PSSPGGRQSA EKSSKKLSSS MQTSARPSQK PQ 

« Hide

Isoform 2 [UniParc].

Checksum: 00B4E309593EC2E9
Show »

FASTA44347,129

References

« Hide 'large scale' references
[1]"Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases."
Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S., Lopez-Otin C.
Biochem. Biophys. Res. Commun. 314:54-62(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 110-1352 (ISOFORM 1), VARIANT THR-538.
Tissue: Cerebellum and Uterus.
[3]"Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O.
DNA Res. 6:337-345(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 626-1352 (ISOFORM 1).
Tissue: Brain.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 539-709 (ISOFORM 1).
Tissue: Uterine endothelium.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ586135 mRNA. Translation: CAE51935.2.
AK126447 mRNA. Translation: BAC86554.1.
AK126752 mRNA. No translation available.
AB033029 mRNA. Translation: BAA86517.1.
BX648357 mRNA. Translation: CAH10383.1.
RefSeqNP_065769.3. NM_020718.3.
UniGeneHs.183817.

3D structure databases

ProteinModelPortalQ70CQ4.
SMRQ70CQ4. Positions 125-314, 593-765.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121548. 2 interactions.
STRING9606.ENSP00000219689.

Protein family/group databases

MEROPSC19.071.

PTM databases

PhosphoSiteQ70CQ4.

Polymorphism databases

DMDM134047944.

Proteomic databases

PaxDbQ70CQ4.
PRIDEQ70CQ4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000219689; ENSP00000219689; ENSG00000103404. [Q70CQ4-1]
GeneID57478.
KEGGhsa:57478.
UCSCuc002dll.3. human. [Q70CQ4-1]

Organism-specific databases

CTD57478.
GeneCardsGC16M022986.
H-InvDBHIX0012889.
HGNCHGNC:20060. USP31.
HPAHPA006937.
neXtProtNX_Q70CQ4.
PharmGKBPA164742780.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5560.
HOGENOMHOG000154758.
HOVERGENHBG094125.
InParanoidQ70CQ4.
KOK11852.
OrthoDBEOG7VMP4B.
PhylomeDBQ70CQ4.
TreeFamTF106278.

Gene expression databases

ArrayExpressQ70CQ4.
BgeeQ70CQ4.
CleanExHS_USP31.
GenevestigatorQ70CQ4.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSUSP31. human.
GenomeRNAi57478.
NextBio63727.
PROQ70CQ4.

Entry information

Entry nameUBP31_HUMAN
AccessionPrimary (citable) accession number: Q70CQ4
Secondary accession number(s): Q6AW97 expand/collapse secondary AC list , Q6ZTC0, Q6ZTN2, Q9ULL7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: March 20, 2007
Last modified: April 16, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM