Q70CQ4 (UBP31_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 31
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 31
Ubiquitin thioesterase 31
Ubiquitin-specific-processing protease 31

Gene names

Name:	USP31
Synonyms:	KIAA1203

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1352 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Tissue specificity	Widely expressed. Ref.1
Sequence similarities	Belongs to the peptidase C19 family.
Sequence caution	The sequence AK126752 differs from that shown. Reason: Frameshift at position 941.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q70CQ4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q70CQ4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-697: Missing.
     698-725: LGRDPEDYIYDLYAVCNHHGTMQGGHYT → MSQRDGVCGSHEVAGAASPGADGGLSLA
     1095-1140: PKKESSPKSQ...KKASGPATRS → TQFPSGEPGP...QVWFEEGQQV
     1141-1352: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1352	1352	Ubiquitin carboxyl-terminal hydrolase 31		PRO_0000249518
Regions
	Compositional bias	95 – 125	31	Pro-rich
	Compositional bias	770 – 1221	452	Ser-rich
Sites
	Active site	137	1	Nucleophile By similarity
	Active site	723	1	Proton acceptor By similarity
Natural variations
	Alternative sequence	1 – 697	697	Missing in isoform 2.		VSP_020459
	Alternative sequence	698 – 725	28	LGRDP…GGHYT → MSQRDGVCGSHEVAGAASPG ADGGLSLA in isoform 2.		VSP_020460
	Alternative sequence	1095 – 1140	46	PKKES…PATRS → TQFPSGEPGPSRGGQGGREA RAELLHGQPALPQHKHQVWF EEGQQV in isoform 2.		VSP_020461
	Alternative sequence	1141 – 1352	212	Missing in isoform 2.		VSP_020462
	Natural variant	445	1	D → Y. Corresponds to variant rs1978066 [ dbSNP | Ensembl ].		VAR_027422
	Natural variant	532	1	Q → H. Corresponds to variant rs4597335 [ dbSNP | Ensembl ].		VAR_027423
	Natural variant	538	1	I → T. Ref.2 Corresponds to variant rs13339649 [ dbSNP | Ensembl ].		VAR_027424
	Natural variant	552	1	A → T. Corresponds to variant rs9932912 [ dbSNP | Ensembl ].		VAR_027425
	Natural variant	931	1	R → L. Corresponds to variant rs10083789 [ dbSNP | Ensembl ].		VAR_027426
	Natural variant	1269	1	D → N. Corresponds to variant rs35541113 [ dbSNP | Ensembl ].		VAR_051534
	Natural variant	1309	1	R → C. Corresponds to variant rs35254998 [ dbSNP | Ensembl ].		VAR_051535
Experimental info
	Sequence conflict	568	1	L → P in CAE51935. Ref.1
	Sequence conflict	624	1	Q → H in AK126752. Ref.2
	Sequence conflict	1082	1	R → S in AK126752. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 20, 2007. Version 2. Checksum: F68A90EC45D23EDF	FASTA	1,352	146,651
	10 20 30 40 50 60 MSKVTAPGSG PPAAASGKEK RSFSKRLFRS GRAGGGGAGG PGASGPAAPS SPSSPSSARS 70 80 90 100 110 120 VGSFMSRVLK TLSTLSHLSS EGAAPDRGGL RSCFPPGPAA APTPPPCPPP PASPAPPACA 130 140 150 160 170 180 AEPVPGVAGL RNHGNTCFMN ATLQCLSNTE LFAEYLALGQ YRAGRPEPSP DPEQPAGRGA 190 200 210 220 230 240 QGQGEVTEQL AHLVRALWTL EYTPQHSRDF KTIVSKNALQ YRGNSQHDAQ EFLLWLLDRV 250 260 270 280 290 300 HEDLNHSVKQ SGQPPLKPPS ETDMMPEGPS FPVCSTFVQE LFQAQYRSSL TCPHCQKQSN 310 320 330 340 350 360 TFDPFLCISL PIPLPHTRPL YVTVVYQGKC SHCMRIGVAV PLSGTVARLR EAVSMETKIP 370 380 390 400 410 420 TDQIVLTEMY YDGFHRSFCD TDDLETVHES DCIFAFETPE IFRPEGILSQ RGIHLNNNLN 430 440 450 460 470 480 HLKFGLDYHR LSSPTQTAAK QGKMDSPTSR AGSDKIVLLV CNRACTGQQG KRFGLPFVLH 490 500 510 520 530 540 LEKTIAWDLL QKEILEKMKY FLRPTVCIQV CPFSLRVVSV VGITYLLPQE EQPLCHPIVE 550 560 570 580 590 600 RALKSCGPGG TAHVKLVVEW DKETRDFLFV NTEDEYIPDA ESVRLQRERH HQPQTCTLSQ 610 620 630 640 650 660 CFQLYTKEER LAPDDAWRCP HCKQLQQGSI TLSLWTLPDV LIIHLKRFRQ EGDRRMKLQN 670 680 690 700 710 720 MVKFPLTGLD MTPHVVKRSQ SSWSLPSHWS PWRRPYGLGR DPEDYIYDLY AVCNHHGTMQ 730 740 750 760 770 780 GGHYTAYCKN SVDGLWYCFD DSDVQQLSED EVCTQTAYIL FYQRRTAIPS WSANSSVAGS 790 800 810 820 830 840 TSSSLCEHWV SRLPGSKPAS VTSAASSRRT SLASLSESVE MTGERSEDDG GFSTRPFVRS 850 860 870 880 890 900 VQRQSLSSRS SVTSPLAVNE NCMRPSWSLS AKLQMRSNSP SRFSGDSPIH SSASTLEKIG 910 920 930 940 950 960 EAADDKVSIS CFGSLRNLSS SYQEPSDSHS RREHKAVGRA PLAVMEGVFK DESDTRRLNS 970 980 990 1000 1010 1020 SVVDTQSKHS AQGDRLPPLS GPFDNNNQIA YVDQSDSVDS SPVKEVKAPS HPGSLAKKPE 1030 1040 1050 1060 1070 1080 STTKRSPSSK GTSEPEKSLR KGRPALASQE SSLSSTSPSS PLPVKVSLKP SRSRSKADSS 1090 1100 1110 1120 1130 1140 SRGSGRHSSP APAQPKKESS PKSQDSVSSP SPQKQKSASA LTYTASSTSA KKASGPATRS 1150 1160 1170 1180 1190 1200 PFPPGKSRTS DHSLSREGSR QSLGSDRASA TSTSKPNSPR VSQARAGEGR GAGKHVRSSS 1210 1220 1230 1240 1250 1260 MASLRSPSTS IKSGLKRDSK SEDKGLSFFK SALRQKETRR STDLGKTALL SKKAGGSSVK 1270 1280 1290 1300 1310 1320 SVCKNTGDDE AERGHQPPAS QQPNANTTGK EQLVTKDPAS AKHSLLSARK SKSSQLDSGV 1330 1340 1350 PSSPGGRQSA EKSSKKLSSS MQTSARPSQK PQ
	Isoform 2 [UniParc]. Checksum: 00B4E309593EC2E9	FASTA	443	47,129
	10 20 30 40 50 60 MSQRDGVCGS HEVAGAASPG ADGGLSLAAY CKNSVDGLWY CFDDSDVQQL SEDEVCTQTA 70 80 90 100 110 120 YILFYQRRTA IPSWSANSSV AGSTSSSLCE HWVSRLPGSK PASVTSAASS RRTSLASLSE 130 140 150 160 170 180 SVEMTGERSE DDGGFSTRPF VRSVQRQSLS SRSSVTSPLA VNENCMRPSW SLSAKLQMRS 190 200 210 220 230 240 NSPSRFSGDS PIHSSASTLE KIGEAADDKV SISCFGSLRN LSSSYQEPSD SHSRREHKAV 250 260 270 280 290 300 GRAPLAVMEG VFKDESDTRR LNSSVVDTQS KHSAQGDRLP PLSGPFDNNN QIAYVDQSDS 310 320 330 340 350 360 VDSSPVKEVK APSHPGSLAK KPESTTKRSP SSKGTSEPEK SLRKGRPALA SQESSLSSTS 370 380 390 400 410 420 PSSPLPVKVS LKPSRSRSKA DSSSRGSGRH SSPAPAQTQF PSGEPGPSRG GQGGREARAE 430 440 LLHGQPALPQ HKHQVWFEEG QQV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases." Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S., Lopez-Otin C. Biochem. Biophys. Res. Commun. 314:54-62(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 110-1352 (ISOFORM 1), VARIANT THR-538. Tissue: Cerebellum and Uterus.
[3]	"Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O. DNA Res. 6:337-345(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 626-1352 (ISOFORM 1). Tissue: Brain.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 539-709 (ISOFORM 1). Tissue: Uterine endothelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ586135 mRNA. Translation: CAE51935.2. AK126447 mRNA. Translation: BAC86554.1. AK126752 mRNA. No translation available. AB033029 mRNA. Translation: BAA86517.1. BX648357 mRNA. Translation: CAH10383.1.
IPI	IPI00297289. IPI00445176.
RefSeq	NP_065769.3. NM_020718.3.
UniGene	Hs.183817.
3D structure databases
ProteinModelPortal	Q70CQ4.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000219689.
Protein family/group databases
MEROPS	C19.071.
PTM databases
PhosphoSite	Q70CQ4.
Polymorphism databases
DMDM	134047944.
Proteomic databases
PaxDb	Q70CQ4.
PRIDE	Q70CQ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000219689; ENSP00000219689; ENSG00000103404.
GeneID	57478.
KEGG	hsa:57478.
UCSC	uc002dll.3. human.
Organism-specific databases
CTD	57478.
GeneCards	GC16M022986.
H-InvDB	HIX0012889.
HGNC	HGNC:20060. USP31.
HPA	HPA006937.
neXtProt	NX_Q70CQ4.
PharmGKB	PA164742780.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5560.
HOGENOM	HOG000154758.
HOVERGEN	HBG094125.
InParanoid	Q70CQ4.
KO	K11852.
OMA	SYQEPSD.
OrthoDB	EOG43TZTQ.
PhylomeDB	Q70CQ4.
Gene expression databases
ArrayExpress	Q70CQ4.
Bgee	Q70CQ4.
CleanEx	HS_USP31.
Genevestigator	Q70CQ4.
GermOnline	ENSG00000103404. Homo sapiens.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	USP31. human.
GenomeRNAi	57478.
NextBio	63727.

Entry information

Entry name

UBP31_HUMAN

Accession

Primary (citable) accession number: Q70CQ4
Secondary accession number(s): Q6AW97 , Q6ZTC0, Q6ZTN2, Q9ULL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	March 20, 2007
Last modified:	May 29, 2013
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.