Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q70CQ1

- UBP49_HUMAN

UniProt

Q70CQ1 - UBP49_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Ubiquitin carboxyl-terminal hydrolase 49

Gene

USP49

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Specifically deubiquitinates histone H2B at 'Lys-120' (H2BK120Ub). H2BK120Ub is a specific tag for epigenetic transcriptional activation and acts as a regulator of mRNA splicing. Deubiquitination is required for efficient cotranscriptional splicing of a large set of exons.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei262 – 2621NucleophileCurated
Active sitei615 – 6151Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri24 – 8562UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: UniProtKB
  2. histone binding Source: UniProtKB
  3. ubiquitin-specific protease activity Source: UniProtKB
  4. ubiquitin thiolesterase activity Source: UniProtKB
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. histone H2B conserved C-terminal lysine deubiquitination Source: UniProtKB
  2. mRNA splicing, via spliceosome Source: UniProtKB
  3. protein deubiquitination Source: FlyBase
  4. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

mRNA processing, mRNA splicing, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.073.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 49 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 49
Ubiquitin thioesterase 49
Ubiquitin-specific-processing protease 49
Gene namesi
Name:USP49
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 6

Organism-specific databases

HGNCiHGNC:20078. USP49.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi262 – 2621C → A: Loss of deubiquitinase activity. 1 Publication

Organism-specific databases

PharmGKBiPA134954570.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 688688Ubiquitin carboxyl-terminal hydrolase 49PRO_0000080678Add
BLAST

Proteomic databases

PaxDbiQ70CQ1.
PRIDEiQ70CQ1.

PTM databases

PhosphoSiteiQ70CQ1.

Expressioni

Gene expression databases

BgeeiQ70CQ1.
CleanExiHS_USP49.
ExpressionAtlasiQ70CQ1. baseline and differential.
GenevestigatoriQ70CQ1.

Organism-specific databases

HPAiHPA030254.
HPA030255.

Interactioni

Subunit structurei

Component of a complex with RUVBL1 and PSMC5.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
FKBP5Q134512EBI-2511022,EBI-306914

Protein-protein interaction databases

BioGridi117382. 38 interactions.
IntActiQ70CQ1. 48 interactions.
STRINGi9606.ENSP00000297229.

Structurei

3D structure databases

ProteinModelPortaliQ70CQ1.
SMRiQ70CQ1. Positions 26-83, 246-655.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini253 – 657405USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri24 – 8562UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00760000119203.
HOGENOMiHOG000015084.
HOVERGENiHBG018027.
InParanoidiQ70CQ1.
KOiK11834.
OMAiCLECATT.
OrthoDBiEOG7JX33S.
PhylomeDBiQ70CQ1.
TreeFamiTF315281.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q70CQ1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDRCKHVGRL RLAQDHSILN PQKWCCLECA TTESVWACLK CSHVACGRYI
60 70 80 90 100
EDHALKHFEE TGHPLAMEVR DLYVFCYLCK DYVLNDNPEG DLKLLRSSLL
110 120 130 140 150
AVRGQKQDTP VRRGRTLRSM ASGEDVVLPQ RAPQGQPQML TALWYRRQRL
160 170 180 190 200
LARTLRLWFE KSSRGQAKLE QRRQEEALER KKEEARRRRR EVKRRLLEEL
210 220 230 240 250
ASTPPRKSAR LLLHTPRDAG PAASRPAALP TSRRVPAATL KLRRQPAMAP
260 270 280 290 300
GVTGLRNLGN TCYMNSILQV LSHLQKFREC FLNLDPSKTE HLFPKATNGK
310 320 330 340 350
TQLSGKPTNS SATELSLRND RAEACEREGF CWNGRASISR SLELIQNKEP
360 370 380 390 400
SSKHISLCRE LHTLFRVMWS GKWALVSPFA MLHSVWSLIP AFRGYDQQDA
410 420 430 440 450
QEFLCELLHK VQQELESEGT TRRILIPFSQ RKLTKQVLKV VNTIFHGQLL
460 470 480 490 500
SQVTCISCNY KSNTIEPFWD LSLEFPERYH CIEKGFVPLN QTECLLTEML
510 520 530 540 550
AKFTETEALE GRIYACDQCN SKRRKSNPKP LVLSEARKQL MIYRLPQVLR
560 570 580 590 600
LHLKRFRWSG RNHREKIGVH VVFDQVLTME PYCCRDMLSS LDKETFAYDL
610 620 630 640 650
SAVVMHHGKG FGSGHYTAYC YNTEGGFWVH CNDSKLNVCS VEEVCKTQAY
660 670 680
ILFYTQRTVQ GNARISETHL QAQVQSSNND EGRPQTFS
Length:688
Mass (Da):79,198
Last modified:July 5, 2004 - v1
Checksum:i1DE97A430A636B0B
GO
Isoform 2 (identifier: Q70CQ1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     627-688: FWVHCNDSKL...NDEGRPQTFS → ACALLCGVGDTERG

Note: No experimental confirmation available.

Show »
Length:640
Mass (Da):73,455
Checksum:i2476376554DA939E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei627 – 68862FWVHC…PQTFS → ACALLCGVGDTERG in isoform 2. 1 PublicationVSP_011556Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ586139 mRNA. Translation: CAE51939.1.
AL365205 Genomic DNA. Translation: CAI13187.1.
AL365205 Genomic DNA. Translation: CAI13188.1.
CH471081 Genomic DNA. Translation: EAX04065.1.
BC014176 mRNA. Translation: AAH14176.1.
CCDSiCCDS4861.1. [Q70CQ1-2]
CCDS69111.1. [Q70CQ1-1]
RefSeqiNP_001273483.1. NM_001286554.1. [Q70CQ1-1]
NP_061031.2. NM_018561.4. [Q70CQ1-2]
UniGeneiHs.593575.
Hs.665742.

Genome annotation databases

EnsembliENST00000373006; ENSP00000362097; ENSG00000164663. [Q70CQ1-2]
ENST00000373009; ENSP00000362100; ENSG00000164663. [Q70CQ1-1]
ENST00000394253; ENSP00000377797; ENSG00000164663. [Q70CQ1-1]
GeneIDi25862.
KEGGihsa:25862.
UCSCiuc003ori.3. human. [Q70CQ1-2]

Polymorphism databases

DMDMi52000871.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ586139 mRNA. Translation: CAE51939.1 .
AL365205 Genomic DNA. Translation: CAI13187.1 .
AL365205 Genomic DNA. Translation: CAI13188.1 .
CH471081 Genomic DNA. Translation: EAX04065.1 .
BC014176 mRNA. Translation: AAH14176.1 .
CCDSi CCDS4861.1. [Q70CQ1-2 ]
CCDS69111.1. [Q70CQ1-1 ]
RefSeqi NP_001273483.1. NM_001286554.1. [Q70CQ1-1 ]
NP_061031.2. NM_018561.4. [Q70CQ1-2 ]
UniGenei Hs.593575.
Hs.665742.

3D structure databases

ProteinModelPortali Q70CQ1.
SMRi Q70CQ1. Positions 26-83, 246-655.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 117382. 38 interactions.
IntActi Q70CQ1. 48 interactions.
STRINGi 9606.ENSP00000297229.

Protein family/group databases

MEROPSi C19.073.

PTM databases

PhosphoSitei Q70CQ1.

Polymorphism databases

DMDMi 52000871.

Proteomic databases

PaxDbi Q70CQ1.
PRIDEi Q70CQ1.

Protocols and materials databases

DNASUi 25862.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000373006 ; ENSP00000362097 ; ENSG00000164663 . [Q70CQ1-2 ]
ENST00000373009 ; ENSP00000362100 ; ENSG00000164663 . [Q70CQ1-1 ]
ENST00000394253 ; ENSP00000377797 ; ENSG00000164663 . [Q70CQ1-1 ]
GeneIDi 25862.
KEGGi hsa:25862.
UCSCi uc003ori.3. human. [Q70CQ1-2 ]

Organism-specific databases

CTDi 25862.
GeneCardsi GC06M041795.
H-InvDB HIX0164985.
HGNCi HGNC:20078. USP49.
HPAi HPA030254.
HPA030255.
neXtProti NX_Q70CQ1.
PharmGKBi PA134954570.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5077.
GeneTreei ENSGT00760000119203.
HOGENOMi HOG000015084.
HOVERGENi HBG018027.
InParanoidi Q70CQ1.
KOi K11834.
OMAi CLECATT.
OrthoDBi EOG7JX33S.
PhylomeDBi Q70CQ1.
TreeFami TF315281.

Miscellaneous databases

GenomeRNAii 25862.
NextBioi 47231.
PROi Q70CQ1.

Gene expression databases

Bgeei Q70CQ1.
CleanExi HS_USP49.
ExpressionAtlasi Q70CQ1. baseline and differential.
Genevestigatori Q70CQ1.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
SMARTi SM00290. ZnF_UBP. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases."
    Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S., Lopez-Otin C.
    Biochem. Biophys. Res. Commun. 314:54-62(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME ACTIVITY.
  2. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Eye.
  5. "USP49 deubiquitinates histone H2B and regulates cotranscriptional pre-mRNA splicing."
    Zhang Z., Jones A., Joo H.Y., Zhou D., Cao Y., Chen S., Erdjument-Bromage H., Renfrow M., He H., Tempst P., Townes T.M., Giles K.E., Ma L., Wang H.
    Genes Dev. 27:1581-1595(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH RUVBL1 AND PSMC5, MUTAGENESIS OF CYS-262.

Entry informationi

Entry nameiUBP49_HUMAN
AccessioniPrimary (citable) accession number: Q70CQ1
Secondary accession number(s): Q5T3D9, Q5T3E0, Q96CK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3