Q70CQ1 (UBP49_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 49
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 49
Ubiquitin thioesterase 49
Ubiquitin-specific-processing protease 49

Gene names

Name:

USP49

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	688 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). Ref.1
Sequence similarities	Belongs to the peptidase C19 family. Contains 1 UBP-type zinc finger.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q70CQ1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q70CQ1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     627-688: FWVHCNDSKL...NDEGRPQTFS → ACALLCGVGDTERG

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 688	688	Ubiquitin carboxyl-terminal hydrolase 49		PRO_0000080678
Regions
	Zinc finger	24 – 85	62	UBP-type
Sites
	Active site	262	1	Nucleophile By similarity
	Active site	615	1	Proton acceptor By similarity
Natural variations
	Alternative sequence	627 – 688	62	FWVHC…PQTFS → ACALLCGVGDTERG in isoform 2.		VSP_011556

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 1DE97A430A636B0B	FASTA	688	79,198
	10 20 30 40 50 60 MDRCKHVGRL RLAQDHSILN PQKWCCLECA TTESVWACLK CSHVACGRYI EDHALKHFEE 70 80 90 100 110 120 TGHPLAMEVR DLYVFCYLCK DYVLNDNPEG DLKLLRSSLL AVRGQKQDTP VRRGRTLRSM 130 140 150 160 170 180 ASGEDVVLPQ RAPQGQPQML TALWYRRQRL LARTLRLWFE KSSRGQAKLE QRRQEEALER 190 200 210 220 230 240 KKEEARRRRR EVKRRLLEEL ASTPPRKSAR LLLHTPRDAG PAASRPAALP TSRRVPAATL 250 260 270 280 290 300 KLRRQPAMAP GVTGLRNLGN TCYMNSILQV LSHLQKFREC FLNLDPSKTE HLFPKATNGK 310 320 330 340 350 360 TQLSGKPTNS SATELSLRND RAEACEREGF CWNGRASISR SLELIQNKEP SSKHISLCRE 370 380 390 400 410 420 LHTLFRVMWS GKWALVSPFA MLHSVWSLIP AFRGYDQQDA QEFLCELLHK VQQELESEGT 430 440 450 460 470 480 TRRILIPFSQ RKLTKQVLKV VNTIFHGQLL SQVTCISCNY KSNTIEPFWD LSLEFPERYH 490 500 510 520 530 540 CIEKGFVPLN QTECLLTEML AKFTETEALE GRIYACDQCN SKRRKSNPKP LVLSEARKQL 550 560 570 580 590 600 MIYRLPQVLR LHLKRFRWSG RNHREKIGVH VVFDQVLTME PYCCRDMLSS LDKETFAYDL 610 620 630 640 650 660 SAVVMHHGKG FGSGHYTAYC YNTEGGFWVH CNDSKLNVCS VEEVCKTQAY ILFYTQRTVQ 670 680 GNARISETHL QAQVQSSNND EGRPQTFS
	Isoform 2 [UniParc]. Checksum: 2476376554DA939E	FASTA	640	73,455
	10 20 30 40 50 60 MDRCKHVGRL RLAQDHSILN PQKWCCLECA TTESVWACLK CSHVACGRYI EDHALKHFEE 70 80 90 100 110 120 TGHPLAMEVR DLYVFCYLCK DYVLNDNPEG DLKLLRSSLL AVRGQKQDTP VRRGRTLRSM 130 140 150 160 170 180 ASGEDVVLPQ RAPQGQPQML TALWYRRQRL LARTLRLWFE KSSRGQAKLE QRRQEEALER 190 200 210 220 230 240 KKEEARRRRR EVKRRLLEEL ASTPPRKSAR LLLHTPRDAG PAASRPAALP TSRRVPAATL 250 260 270 280 290 300 KLRRQPAMAP GVTGLRNLGN TCYMNSILQV LSHLQKFREC FLNLDPSKTE HLFPKATNGK 310 320 330 340 350 360 TQLSGKPTNS SATELSLRND RAEACEREGF CWNGRASISR SLELIQNKEP SSKHISLCRE 370 380 390 400 410 420 LHTLFRVMWS GKWALVSPFA MLHSVWSLIP AFRGYDQQDA QEFLCELLHK VQQELESEGT 430 440 450 460 470 480 TRRILIPFSQ RKLTKQVLKV VNTIFHGQLL SQVTCISCNY KSNTIEPFWD LSLEFPERYH 490 500 510 520 530 540 CIEKGFVPLN QTECLLTEML AKFTETEALE GRIYACDQCN SKRRKSNPKP LVLSEARKQL 550 560 570 580 590 600 MIYRLPQVLR LHLKRFRWSG RNHREKIGVH VVFDQVLTME PYCCRDMLSS LDKETFAYDL 610 620 630 640 SAVVMHHGKG FGSGHYTAYC YNTEGGACAL LCGVGDTERG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases." Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S., Lopez-Otin C. Biochem. Biophys. Res. Commun. 314:54-62(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME ACTIVITY.
[2]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Eye.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ586139 mRNA. Translation: CAE51939.1. AL365205 Genomic DNA. Translation: CAI13187.1. AL365205 Genomic DNA. Translation: CAI13188.1. CH471081 Genomic DNA. Translation: EAX04065.1. BC014176 mRNA. Translation: AAH14176.1.
IPI	IPI00156871. IPI00455818.
RefSeq	NP_061031.2. NM_018561.3.
UniGene	Hs.665742.
3D structure databases
ProteinModelPortal	Q70CQ1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q70CQ1. 33 interactions.
STRING	9606.ENSP00000297229.
Protein family/group databases
MEROPS	C19.073.
PTM databases
PhosphoSite	Q70CQ1.
Polymorphism databases
DMDM	52000871.
Proteomic databases
PaxDb	Q70CQ1.
PRIDE	Q70CQ1.
Protocols and materials databases
DNASU	25862.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000297229; ENSP00000297229; ENSG00000164663. ENST00000373006; ENSP00000362097; ENSG00000164663. ENST00000373009; ENSP00000362100; ENSG00000164663. ENST00000394253; ENSP00000377797; ENSG00000164663.
GeneID	25862.
KEGG	hsa:25862.
UCSC	uc003ori.3. human.
Organism-specific databases
CTD	25862.
GeneCards	GC06M041762.
H-InvDB	HIX0164985.
HGNC	HGNC:20078. USP49.
HPA	HPA030254. HPA030255.
neXtProt	NX_Q70CQ1.
PharmGKB	PA134954570.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5077.
HOGENOM	HOG000015084.
HOVERGEN	HBG018027.
InParanoid	Q70CQ1.
KO	K11834.
OMA	VRGQKQD.
OrthoDB	EOG4XGZZK.
Gene expression databases
ArrayExpress	Q70CQ1.
Bgee	Q70CQ1.
CleanEx	HS_USP49.
Genevestigator	Q70CQ1.
GermOnline	ENSG00000164663. Homo sapiens.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR013083. Znf_RING/FYVE/PHD. IPR001607. Znf_UBP. [Graphical view]
Pfam	PF00443. UCH. 1 hit. PF02148. zf-UBP. 1 hit. [Graphical view]
SMART	SM00290. ZnF_UBP. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	25862.
NextBio	47231.

Entry information

Entry name

UBP49_HUMAN

Accession

Primary (citable) accession number: Q70CQ1
Secondary accession number(s): Q5T3D9, Q5T3E0, Q96CK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.