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Protein

Enolase

Gene

ENO

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (KLLA0_F09141g), Glyceraldehyde-3-phosphate dehydrogenase 1 (GAP1), Glyceraldehyde-3-phosphate dehydrogenase 2 (GAP2)
  2. Phosphoglycerate kinase (PGK)
  3. Phosphoglycerate mutase (KLLA0_E11859g), Phosphoglycerate mutase (KLLA0_C04081g)
  4. Enolase (ENO)
  5. Pyruvate kinase (PYK1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei160SubstrateBy similarity1
Binding sitei169SubstrateBy similarity1
Active sitei212Proton donorBy similarity1
Metal bindingi247MagnesiumBy similarity1
Metal bindingi296MagnesiumBy similarity1
Binding sitei296SubstrateBy similarity1
Metal bindingi321MagnesiumBy similarity1
Binding sitei321SubstrateBy similarity1
Active sitei346Proton acceptorBy similarity1
Binding sitei397SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:ENO
Synonyms:RAG17
Ordered Locus Names:KLLA0A09185g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340521 – 437EnolaseAdd BLAST437

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi284590.XP_451402.1

Structurei

3D structure databases

ProteinModelPortaliQ70CP7
SMRiQ70CP7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni373 – 376Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670 Eukaryota
COG0148 LUCA
HOGENOMiHOG000072174
InParanoidiQ70CP7
KOiK01689
OMAiEFMIIPV
OrthoDBiEOG092C2W5X

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.1201 hit
3.30.390.101 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q70CP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPISKVYARS VYDSRGNPTV EVELTTEKGT FRSIVPSGAS TGVHEALELR
60 70 80 90 100
DGDKSKWLGK GVLTAVSNVN NIIAPALVKA NVDVTNQQQV DDLLLSLDGT
110 120 130 140 150
ANKSKLGANA ILGVSLAASK AGAAAKNVPL YQHLADLAGA KTQPFVLPVP
160 170 180 190 200
FLNVLNGGSH AGGALALQEF MIAPTGAETF AEALRMGSEV YHNLKSLTKK
210 220 230 240 250
RYGASAGNVG DEGGVAPNIQ TAEEALDLIV DAIKAAGYEG KIDIGLDCAS
260 270 280 290 300
SEFYKDGKYD LDFKNPESDP SKWLSGPQLA DLYHELVKKY PIVSIEDPFA
310 320 330 340 350
EDDWEAWSHF YKTAGVQIVA DDLTVTNPVR IATAIEKKAA DALLLKVNQI
360 370 380 390 400
GSLSESIKAA NDSFAANWGV MVSHRSGETE DTFIADLVVG LRTGQIKTGA
410 420 430
PARSERLAKL NQLLRIEEEL GSNAIFAGKK FHNGNQL
Length:437
Mass (Da):46,518
Last modified:July 5, 2004 - v1
Checksum:iD5C7BC7229C63E1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ586240 Genomic DNA Translation: CAE51943.1
CR382121 Genomic DNA Translation: CAH02990.1
RefSeqiXP_451402.1, XM_451402.1

Genome annotation databases

EnsemblFungiiCAH02990; CAH02990; KLLA0_A09185g
GeneIDi2896838
KEGGikla:KLLA0A09185g

Similar proteinsi

Entry informationi

Entry nameiENO_KLULA
AccessioniPrimary (citable) accession number: Q70CP7
Secondary accession number(s): Q6CXD7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: July 5, 2004
Last modified: February 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome