Q70C53 (Q70C53_SOLTU) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
1,3-beta-D-glucan glucanohydrolase (Glucan endo-1,3-beta-glucosidase a) EMBL CAE52322.1
EC=3.2.1.39 EMBL CAE52322.1

Gene names

Name:

gluB20-2 EMBL CAE52322.1

Organism

Solanum tuberosum (Potato) EMBL CAE52322.1

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the glycosyl hydrolase 17 family. RuleBase RU004335

Ontologies

Keywords
Domain	Signal EMBL CAE52322.1
Molecular function	Glycosidase RuleBase RU004336 EMBL CAE52322.1 Hydrolase
Technical term	3D-structure PDB 3UR7 PDB 3UR8 PDB 4GZI PDB 4GZJ
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential EMBL CAE52322.1
	Chain	24 – 338	315	1,3-beta-D-glucan glucanohydrolase EMBL CAE52322.1		PRO_5000072206

Sequences

	Sequence		Length	Mass (Da)
	Q70C53 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: A5337C9AF69DF3AD	FASTA	338	37,855
	10 20 30 40 50 60 MAFLSSLVVS LLLVGLLIQI TGAQPIGVCY GKIANNLPSD QDVIKLYNAN NIKKMRIYYP 70 80 90 100 110 120 HTNVFNALKG SNIEIILDVP NQDLEALANP SNANGWVQDN IRNHFPDVKF KYIAVGNEVD 130 140 150 160 170 180 PGRESGKYAR FVGPAMENIY NALSSAGLQN QIKVSTSTYS GLLTNTYPPR DSIFREEYKS 190 200 210 220 230 240 FINPIIGFLA RHNLPLLANI YPYFGHIDNT NAVPLSYALF NQQRRNDTGY QNLFDALVDS 250 260 270 280 290 300 MYFATEKLGG QNIEIIVSES GWPSEGHPAA TLKNARTYYT NLINHVKRGA GTPKKPGKTI 310 320 330 ETYLFAMFDE NEKKGEASEK HFGLFNPDQR PKYQLNFN

References

[1]	"Characterization of dual function of 1,3-beta-glucanase product of gluB20-2 gene isolated from potato plants." Barabasz A., Krzymowska M., Bieniak B., Charzynska M., Hennig J. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Tissue: Leaf EMBL CAE52322.1.
[2]	"Functional analysis of the 1,3-beta-glucanase, product of gluB20-2 gene in potato." Barabasz A. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Tissue: Leaf EMBL CAE52322.1.
[3]	"Two high-resolution structures of potato endo-1,3-?-glucanase reveal subdomain flexibility with implications for substrate binding." Wojtkowiak A., Witek K., Hennig J., Jaskolski M. Acta Crystallogr. D 68:713-723(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.26 ANGSTROMS) OF 24-338.
[4]	"Structures of an active-site mutant of a plant 1,3-?-glucanase in complex with oligosaccharide products of hydrolysis." Wojtkowiak A., Witek K., Hennig J., Jaskolski M. Acta Crystallogr. D 69:52-62(2013) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF 24-338.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ586575 mRNA. Translation: CAE52322.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3UR7	X-ray	1.40	A/B	24-338	[»]
3UR8	X-ray	1.26	A/B	24-338	[»]
4GZI	X-ray	1.68	A	24-338	[»]
4GZJ	X-ray	1.55	A	24-338	[»]

HSSP

HSSP built from PDB template 1AQ0 based on UniProtKB P12257.

ProteinModelPortal

Q70C53.

ModBase

Search...

Protein family/group databases

CAZy

GH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.20.20.80. 1 hit.

InterPro

IPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

Pfam

PF00332. Glyco_hydro_17. 1 hit.
[Graphical view]

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.

PROSITE

PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q70C53_SOLTU

Accession

Primary (citable) accession number: Q70C53

Entry history

Integrated into UniProtKB/TrEMBL:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)