Q708Y0 (EBF2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
EIN3-binding F-box protein 2

Gene names

Name:	EBF2
Ordered Locus Names:	At5g25350
ORF Names:	F18G18.90

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of SCF(EBF1) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins (probably including EIN3 and EIL1). Regulator of the ethylene signaling cascade by modulating the stability of EIN3 and EIL1 proteins. Ref.1
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with CUL1, SKP1A/ASK1, SKP1B/ASK2, EIN3, and EIL1. Ref.1
Subcellular location	Nucleus Ref.1.
Tissue specificity	Ubiquitous. Ref.1
Induction	EIN3-dependent induction by ethylene. Ref.1
Sequence similarities	Contains 1 F-box domain. Contains 18 LRR (leucine-rich) repeats.

Ontologies

Keywords
Biological process	Ethylene signaling pathway Ubl conjugation pathway
Cellular component	Nucleus
Domain	Leucine-rich repeat Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ethylene mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of ethylene mediated signaling pathway Traceable author statement. Source: TAIR ubiquitin-dependent protein catabolic process Traceable author statement. Source: TAIR
Cellular component	SCF ubiquitin ligase complex Inferred from physical interaction Ref.1. Source: TAIR nucleus Inferred from direct assay Ref.1. Source: TAIR
Molecular function	protein binding Inferred from physical interaction Ref.1. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EIN3	O24606	3	EBI-593623,EBI-593576
SRK2A	P43291	2	EBI-593623,EBI-401164

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 623	623	EIN3-binding F-box protein 2		PRO_0000272246
Regions
	Domain	52 – 106	55	F-box
	Repeat	119 – 147	29	LRR 1
	Repeat	151 – 176	26	LRR 2
	Repeat	177 – 202	26	LRR 3
	Repeat	203 – 228	26	LRR 4
	Repeat	229 – 254	26	LRR 5
	Repeat	255 – 281	27	LRR 6
	Repeat	307 – 334	28	LRR 7
	Repeat	335 – 360	26	LRR 8
	Repeat	361 – 386	26	LRR 9
	Repeat	387 – 413	27	LRR 10
	Repeat	414 – 441	28	LRR 11
	Repeat	442 – 467	26	LRR 12
	Repeat	468 – 494	27	LRR 13
	Repeat	495 – 521	27	LRR 14
	Repeat	522 – 547	26	LRR 15
	Repeat	548 – 574	27	LRR 16
	Repeat	575 – 600	26	LRR 17
	Repeat	601 – 623	23	LRR 18

Sequences

	Sequence		Length	Mass (Da)
	Q708Y0 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 990E2D9B098A11B6	FASTA	623	66,061
	10 20 30 40 50 60 MSGIFRFSGD EDCLLGGSMY LSPGSCPGVY YPARKRLRVA ATSFYSGFEE KQTSIDVLPE 70 80 90 100 110 120 ECLFEILRRL PSGQERSACA CVSKHWLNLL SSISRSEVNE SSVQDVEEGE GFLSRSLEGK 130 140 150 160 170 180 KATDLRLAAI AVGTSSRGGL GKLQIRGSGF ESKVTDVGLG AVAHGCPSLR IVSLWNLPAV 190 200 210 220 230 240 SDLGLSEIAR SCPMIEKLDL SRCPGITDSG LVAIAENCVN LSDLTIDSCS GVGNEGLRAI 250 260 270 280 290 300 ARRCVNLRSI SIRSCPRIGD QGVAFLLAQA GSYLTKVKLQ MLNVSGLSLA VIGHYGAAVT 310 320 330 340 350 360 DLVLHGLQGV NEKGFWVMGN AKGLKKLKSL SVMSCRGMTD VGLEAVGNGC PDLKHVSLNK 370 380 390 400 410 420 CLLVSGKGLV ALAKSALSLE SLKLEECHRI NQFGLMGFLM NCGSKLKAFS LANCLGISDF 430 440 450 460 470 480 NSESSLPSPS CSSLRSLSIR CCPGFGDASL AFLGKFCHQL QDVELCGLNG VTDAGVRELL 490 500 510 520 530 540 QSNNVGLVKV NLSECINVSD NTVSAISVCH GRTLESLNLD GCKNITNASL VAVAKNCYSV 550 560 570 580 590 600 NDLDISNTLV SDHGIKALAS SPNHLNLQVL SIGGCSSITD KSKACIQKLG RTLLGLNIQR 610 620 CGRISSSTVD TLLENLWRCD ILY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"EIN3-dependent regulation of plant ethylene hormone signaling by two Arabidopsis F box proteins: EBF1 and EBF2." Potuschak T., Lechner E., Parmentier Y., Yanagisawa S., Grava S., Koncz C., Genschik P. Cell 115:679-689(2003) [PubMed: 14675533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH CUL1; SKP1A/ASK1; SKP1B/ASK2; EIN3 AND EIL1.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ609239 Genomic RNA. Translation: CAE75865.1. AY485830 mRNA. Translation: AAR27072.1. AC006258 Genomic DNA. No translation available. CP002688 Genomic DNA. Translation: AED93429.1. AK227858 mRNA. Translation: BAE99835.1.
IPI	IPI00517412.
RefSeq	NP_197917.1. NM_122444.3.
UniGene	At.19865. At.72267.
3D structure databases
ProteinModelPortal	Q708Y0.
SMR	Q708Y0. Positions 53-96, 152-240, 350-386, 405-442, 485-620.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q708Y0. 6 interactions.
STRING	Q708Y0.
Proteomic databases
PRIDE	Q708Y0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G25350.1; AT5G25350.1; AT5G25350.
GeneID	832607.
GenomeReviews	Gene locus AT5G25350 in contig BA000015_GR.
KEGG	ath:AT5G25350.
NMPDR	fig|3702.1.peg.24733.
Organism-specific databases
TAIR	At5g25350.
Phylogenomic databases
eggNOG	KOG1947.
GeneTree	EPGT00070000029268.
HOGENOM	HBG316633.
InParanoid	Q708Y0.
OMA	MGNAKGL.
PhylomeDB	Q708Y0.
ProtClustDB	CLSN2916408.
Gene expression databases
Genevestigator	Q708Y0.
Family and domain databases
InterPro	IPR001810. F-box_dom_cyclin-like. IPR022364. F-box_dom_Skp2-like. IPR006553. Leu-rich_rpt_Cys-con_subtyp. [Graphical view]
KO	K14515.
Pfam	PF00646. F-box. 1 hit. [Graphical view]
SMART	SM00256. FBOX. 1 hit. SM00367. LRR_CC. 1 hit. [Graphical view]
SUPFAM	SSF81383. F-box_dom_Skp2-like. 1 hit.
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EBF2_ARATH

Accession

Primary (citable) accession number: Q708Y0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program