Reviewed, UniProtKB/Swiss-Prot Q708Y0 (EBF2_ARATH)

Names and origin

Protein names

Recommended name:
    EIN3-binding F-box protein 2

Gene names

Name:	EBF2
Ordered Locus Names:	At5g25350
ORF Names:	F18G18.90

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of SCF(EBF1) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins (probably including EIN3 and EIL1). Regulator of the ethylene signaling cascade by modulating the stability of EIN3 and EIL1 proteins.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with CUL1, SKP1A/ASK1, SKP1B/ASK2, EIN3, and EIL1.
Subcellular location	Nucleus.
Tissue specificity	Ubiquitous.
Induction	EIN3-dependent induction by ethylene.
Sequence similarities	Contains 1 F-box domain. Contains 16 LRR (leucine-rich) repeats.

Ontologies

Keywords
Biological process	Ethylene signaling pathway Ubl conjugation pathway
Cellular component	Nucleus
Domain	Leucine-rich repeat Repeat
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ethylene mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Ref.1 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CUL1	Q94AH6	1	EBI-593623,EBI-532411
EIN3	O24606	1	EBI-593623,EBI-593576
SRK2A	P43291	1	EBI-593623,EBI-401164

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 623	623	EIN3-binding F-box protein 2		PRO_0000272246
Regions
	Domain	52 – 106	55	F-box
	Repeat	69 – 86	18	LRR 1
	Repeat	87 – 138	52	LRR 2
	Repeat	139 – 166	28	LRR 3
	Repeat	167 – 193	27	LRR 4
	Repeat	194 – 236	43	LRR 5
	Repeat	237 – 263	27	LRR 6
	Repeat	264 – 287	24	LRR 7
	Repeat	288 – 312	25	LRR 8
	Repeat	313 – 341	29	LRR 9
	Repeat	342 – 367	26	LRR 10
	Repeat	368 – 392	25	LRR 11
	Repeat	393 – 448	56	LRR 12
	Repeat	449 – 475	27	LRR 13
	Repeat	476 – 503	28	LRR 14
	Repeat	504 – 525	22	LRR 15
	Repeat	526 – 549	24	LRR 16

Sequences

	Sequence		Length	Mass (Da)
	Q708Y0-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 990E2D9B098A11B6	FASTA	623	66,061
	10 20 30 40 50 60 MSGIFRFSGD EDCLLGGSMY LSPGSCPGVY YPARKRLRVA ATSFYSGFEE KQTSIDVLPE 70 80 90 100 110 120 ECLFEILRRL PSGQERSACA CVSKHWLNLL SSISRSEVNE SSVQDVEEGE GFLSRSLEGK 130 140 150 160 170 180 KATDLRLAAI AVGTSSRGGL GKLQIRGSGF ESKVTDVGLG AVAHGCPSLR IVSLWNLPAV 190 200 210 220 230 240 SDLGLSEIAR SCPMIEKLDL SRCPGITDSG LVAIAENCVN LSDLTIDSCS GVGNEGLRAI 250 260 270 280 290 300 ARRCVNLRSI SIRSCPRIGD QGVAFLLAQA GSYLTKVKLQ MLNVSGLSLA VIGHYGAAVT 310 320 330 340 350 360 DLVLHGLQGV NEKGFWVMGN AKGLKKLKSL SVMSCRGMTD VGLEAVGNGC PDLKHVSLNK 370 380 390 400 410 420 CLLVSGKGLV ALAKSALSLE SLKLEECHRI NQFGLMGFLM NCGSKLKAFS LANCLGISDF 430 440 450 460 470 480 NSESSLPSPS CSSLRSLSIR CCPGFGDASL AFLGKFCHQL QDVELCGLNG VTDAGVRELL 490 500 510 520 530 540 QSNNVGLVKV NLSECINVSD NTVSAISVCH GRTLESLNLD GCKNITNASL VAVAKNCYSV 550 560 570 580 590 600 NDLDISNTLV SDHGIKALAS SPNHLNLQVL SIGGCSSITD KSKACIQKLG RTLLGLNIQR 610 620 CGRISSSTVD TLLENLWRCD ILY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"EIN3-dependent regulation of plant ethylene hormone signaling by two Arabidopsis F box proteins: EBF1 and EBF2." Potuschak T., Lechner E., Parmentier Y., Yanagisawa S., Grava S., Koncz C., Genschik P. Cell 115:679-689(2003) [PubMed: 14675533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH CUL1; SKP1A/ASK1; SKP1B/ASK2; EIN3 AND EIL1.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ609239 Genomic RNA. Translation: CAE75865.1. AY485830 mRNA. Translation: AAR27072.1. AC006258 Genomic DNA. No translation available. AK227858 mRNA. Translation: BAE99835.1.
RefSeq	NP_197917.1.
UniGene	At.19865
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q708Y0.
Genome annotation databases
GeneID	832607.
GenomeReviews	Gene locus AT5G25350 in contig BA000015_GR.
KEGG	ath:AT5G25350.
NMPDR	fig|3702.1.peg.24733.
Organism-specific databases
TAIR	At5g25350.
Family and domain databases
InterPro	IPR001810. F-box. IPR001611. LRR. IPR006553. LRR_cys_sub-typ. [Graphical view]
Pfam	PF00646. F-box. 1 hit. PF00560. LRR_1. 3 hits. [Graphical view]
SMART	SM00256. FBOX. 1 hit. SM00367. LRR_CC. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EBF2_ARATH

Accession

Primary (citable) accession number: Q708Y0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)