Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Acid-sensing ion channel 1

Gene

asic1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proton-gated sodium channel; it is activated by a drop of the extracellular pH and then becomes rapidly desensitized. Generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Can also transport potassium ions, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions.1 Publication

Enzyme regulationi

Inhibited by the diuretic amiloride.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei76 – 761Important for channel gatingBy similarity
Sitei84 – 841Important for channel desensitizingBy similarity
Sitei291 – 2911Important for channel gatingBy similarity

GO - Molecular functioni

  • acid-sensing ion channel activity Source: UniProtKB
  • cation channel activity Source: ZFIN
  • ligand-gated sodium channel activity Source: ZFIN

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Sodium channel

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiR-DRE-2672351. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Acid-sensing ion channel 1
Short name:
ASIC1
Alternative name(s):
Acid-sensing ion channel 1.2
Amiloride-sensitive cation channel 2-B, neuronal
ZASIC1.2
Gene namesi
Name:asic1
Synonyms:accn2b
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 8

Organism-specific databases

ZFINiZDB-GENE-040513-2. asic1a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5050CytoplasmicBy similarityAdd
BLAST
Transmembranei51 – 7424HelicalBy similarityAdd
BLAST
Topological domaini75 – 429355ExtracellularBy similarityAdd
BLAST
Transmembranei430 – 45627Discontinuously helicalBy similarityAdd
BLAST
Topological domaini457 – 50145CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: ZFIN
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501Acid-sensing ion channel 1PRO_0000181297Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi98 ↔ 199By similarity
Glycosylationi164 – 1641N-linked (GlcNAc...)Sequence analysis
Disulfide bondi177 ↔ 184By similarity
Disulfide bondi294 ↔ 369By similarity
Disulfide bondi312 ↔ 365By similarity
Disulfide bondi316 ↔ 363By similarity
Disulfide bondi325 ↔ 347By similarity
Disulfide bondi327 ↔ 339By similarity
Glycosylationi370 – 3701N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ708S7.
PRIDEiQ708S7.

Expressioni

Tissue specificityi

Expressed in central nervous system. Faintly expressed in the trunk, presumably in dorsal root ganglia.1 Publication

Developmental stagei

First detected 48 hours post-fertilization (hpf) in the ventral thalamus, ventral midbrain, ventral cerebellum, ventral hindbrain, dorsal thalamus, hypothalamus, telencephalon, along the tract of the anterior commissure. Weakly expressed in the dorsal midbrain and olfactory bulb. Expression increases by 96 hpf and is detected in the tectum and trunk.1 Publication

Interactioni

Subunit structurei

Homotrimer or heterotrimer with other ASIC proteins (By similarity). Interacts with asic1/accn2c.By similarity1 Publication

Protein-protein interaction databases

STRINGi7955.ENSDARP00000011782.

Structurei

3D structure databases

ProteinModelPortaliQ708S7.
SMRiQ708S7. Positions 47-458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi446 – 4483Selectivity filterCurated

Domaini

Channel opening involves a conformation change that affects primarily the extracellular domain and the second transmembrane helix and its orientation in the membrane. In the open state, the second transmembrane helix is nearly perpendicular to the plane of the membrane; in the desensitized state it is strongly tilted. Besides, the second transmembrane domain is discontinuously helical in the open state. The GAS motif of the selectivity filter is in an extended conformation, giving rise to a distinct kink in the polypeptide chain. A domain swap between subunits gives rise to a full-length transmembrane helix (By similarity).By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4294. Eukaryota.
ENOG410ZNFK. LUCA.
GeneTreeiENSGT00760000119120.
HOGENOMiHOG000247010.
HOVERGENiHBG004150.
KOiK04829.
OMAiDEPSFID.
OrthoDBiEOG091G053J.
PhylomeDBiQ708S7.
TreeFamiTF330663.

Family and domain databases

InterProiIPR004724. ENaC.
IPR001873. Na+channel_ASC.
IPR020903. Na+channel_ASC_CS.
[Graphical view]
PANTHERiPTHR11690. PTHR11690. 1 hit.
PfamiPF00858. ASC. 1 hit.
[Graphical view]
PRINTSiPR01078. AMINACHANNEL.
TIGRFAMsiTIGR00859. ENaC. 1 hit.
PROSITEiPS01206. ASC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q708S7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTSVMDLKV EPMDIDFDQP PPLQVFAHTS TLHGISHIFS YEKITAKCCL
60 70 80 90 100
WVVFFLSSLT FLMYVCIDRI QFYLEYPHVT KLDEITTPVM VFPAVTICNL
110 120 130 140 150
NSIRFSRITR NDLYHAGELL ALLNSRHEVR EAHLVEESVM EVLKSKTDFR
160 170 180 190 200
SFKPRHFNMW EFYNRTGHDI KDMLLSCQFR GSPCRPEDFS VVFTRYGKCY
210 220 230 240 250
TFNSGETGPP RVSVKGGMGN GLEIMLDIQQ DEYLPVWGES DESSFEAGIK
260 270 280 290 300
VQIHSQDEPP FIDQLGFGVA PGFQTFVSCQ EQRLVYLPAP WGSCKSTPPS
310 320 330 340 350
SDYFRAYSIS ACRTDCETRY LVENCNCRMV HMPGDAPYCT PVLYKECAHP
360 370 380 390 400
ALDFLVETDS DYCSCETPCN ITRYSKELSF VKIPSKASVK YLAKKYSKSE
410 420 430 440 450
KYITENVMVL DVFFEALNYE TIEQRKAYEV AGLLGDIGGQ MGLFIGASIL
460 470 480 490 500
TILELFDYLY EVMKYRLCRC SNKKHHNNNN NTDHNAVFSL DDVNCHVSKF

H
Length:501
Mass (Da):57,419
Last modified:July 5, 2004 - v1
Checksum:i5CF87EC031A87CF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ609616 mRNA. Translation: CAE81919.1.
RefSeqiNP_999955.1. NM_214790.2.
XP_009302015.1. XM_009303740.1.
UniGeneiDr.98481.

Genome annotation databases

EnsembliENSDART00000004588; ENSDARP00000011782; ENSDARG00000008329.
GeneIDi791696.
KEGGidre:791696.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ609616 mRNA. Translation: CAE81919.1.
RefSeqiNP_999955.1. NM_214790.2.
XP_009302015.1. XM_009303740.1.
UniGeneiDr.98481.

3D structure databases

ProteinModelPortaliQ708S7.
SMRiQ708S7. Positions 47-458.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000011782.

Proteomic databases

PaxDbiQ708S7.
PRIDEiQ708S7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000004588; ENSDARP00000011782; ENSDARG00000008329.
GeneIDi791696.
KEGGidre:791696.

Organism-specific databases

CTDi791696.
ZFINiZDB-GENE-040513-2. asic1a.

Phylogenomic databases

eggNOGiKOG4294. Eukaryota.
ENOG410ZNFK. LUCA.
GeneTreeiENSGT00760000119120.
HOGENOMiHOG000247010.
HOVERGENiHBG004150.
KOiK04829.
OMAiDEPSFID.
OrthoDBiEOG091G053J.
PhylomeDBiQ708S7.
TreeFamiTF330663.

Enzyme and pathway databases

ReactomeiR-DRE-2672351. Stimuli-sensing channels.

Miscellaneous databases

PROiQ708S7.

Family and domain databases

InterProiIPR004724. ENaC.
IPR001873. Na+channel_ASC.
IPR020903. Na+channel_ASC_CS.
[Graphical view]
PANTHERiPTHR11690. PTHR11690. 1 hit.
PfamiPF00858. ASC. 1 hit.
[Graphical view]
PRINTSiPR01078. AMINACHANNEL.
TIGRFAMsiTIGR00859. ENaC. 1 hit.
PROSITEiPS01206. ASC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASI1B_DANRE
AccessioniPrimary (citable) accession number: Q708S7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.