Q704B2 (MDH_THETK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	TTX_1427

Organism

Thermoproteus tenax (strain ATCC 35583 / NBRC 100435 / JCM 9277 / Kra 1) [Complete proteome] [HAMAP]

Taxonomic identifier

768679 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Thermoproteus › Thermoproteus tenax

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Malate dehydrogenase HAMAP-Rule MF_00487		PRO_0000113496
Regions
	Nucleotide binding	6 – 11	6	NAD By similarity
	Nucleotide binding	116 – 118	3	NAD By similarity
Sites
	Active site	173	1	Proton acceptor By similarity
	Binding site	31	1	NAD By similarity
	Binding site	80	1	Substrate By similarity
	Binding site	86	1	Substrate By similarity
	Binding site	93	1	NAD By similarity
	Binding site	118	1	Substrate By similarity
	Binding site	149	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q704B2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 6DB990BB5CFF19F2	FASTA	308	32,861
	10 20 30 40 50 60 MITVVGSGRV GATTAAMLGV LGVDNKIVLI DIIKGLPQGE ALDLNHMSSI LGLDVYYTGS 70 80 90 100 110 120 NDYADMKGSD LVIVTAGLAR KPGMTREQLL EQNAQIVANI GKEIAKYAPD SVVILTTNPL 130 140 150 160 170 180 DAMTYVMWRA TGFSRERVVG FSGVLDGGRL AFYAGQKLGI SPASIIPIVL GQHGESMFPV 190 200 210 220 230 240 PSKSFVFGVP LDKLLKPEEI KEAVEETVKA GARITELRGF SSNWAPGAGV AIMAKAVKRD 250 260 270 280 290 300 ERRALIASVV LDGEYGVRGI PVEVPVVLGR GGAIKVLEVE LSPEEKQRFQ QSVEAISKLL NSLPAQYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Reconstruction of the central carbohydrate metabolism of Thermoproteus tenax using genomic and biochemical data." Siebers B., Tjaden B., Michalke K., Doerr C., Ahmed H., Zaparty M., Gordon P., Sensen C.W., Zibat A., Klenk H.-P., Schuster S.C., Hensel R. J. Bacteriol. 186:2179-2194(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35583 / NBRC 100435 / JCM 9277 / Kra 1.
[2]	"The complete genome sequence of Thermoproteus tenax: a physiologically versatile member of the Crenarchaeota." Siebers B., Zaparty M., Raddatz G., Tjaden B., Albers S.V., Bell S.D., Blombach F., Kletzin A., Kyrpides N., Lanz C., Plagens A., Rampp M., Rosinus A., von Jan M., Makarova K.S., Klenk H.P., Schuster S.C., Hensel R. PLoS ONE 6:E24222-E24222(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35583 / NBRC 100435 / JCM 9277 / Kra 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ621301 Genomic DNA. Translation: CAF18482.1. FN869859 Genomic DNA. Translation: CCC82058.1.
RefSeq	YP_004893136.1. NC_016070.1.
3D structure databases
ProteinModelPortal	Q704B2.
SMR	Q704B2. Positions 1-308.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CCC82058; CCC82058; TTX_1427.
GeneID	11262306.
KEGG	ttn:TTX_1427.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K00024.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_THETK

Accession

Primary (citable) accession number: Q704B2
Secondary accession number(s): G4RKG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program