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Q703I2

- ALF_THECA

UniProt

Q703I2 - ALF_THECA

Protein

Fructose-bisphosphate aldolase

Gene

fba

Organism
Thermus caldophilus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.Curated

    Cofactori

    Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei49 – 491Glyceraldehyde 3-phosphateBy similarity
    Active sitei80 – 801Proton donorBy similarity
    Metal bindingi81 – 811Zinc 1; catalyticBy similarity
    Metal bindingi102 – 1021Zinc 2By similarity
    Metal bindingi132 – 1321Zinc 2By similarity
    Metal bindingi178 – 1781Zinc 1; catalyticBy similarity
    Binding sitei179 – 1791Dihydroxyacetone phosphate; via amide nitrogenBy similarity
    Metal bindingi208 – 2081Zinc 1; catalyticBy similarity

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. fructose 1,6-bisphosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase (EC:4.1.2.13)
    Short name:
    FBP aldolase
    Short name:
    FBPA
    Alternative name(s):
    Fructose-1,6-bisphosphate aldolase
    Gene namesi
    Name:fbaImported
    OrganismiThermus caldophilus
    Taxonomic identifieri272 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 305305Fructose-bisphosphate aldolasePRO_0000178752Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Structurei

    Secondary structure

    1
    305
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 149
    Beta strandi19 – 235
    Helixi27 – 3812
    Helixi39 – 413
    Beta strandi44 – 474
    Helixi50 – 6819
    Beta strandi75 – 784
    Helixi85 – 939
    Beta strandi97 – 1015
    Helixi108 – 12417
    Beta strandi128 – 1347
    Helixi146 – 1505
    Helixi155 – 16410
    Beta strandi178 – 1814
    Beta strandi184 – 1863
    Helixi191 – 20010
    Beta strandi205 – 2073
    Helixi215 – 2239
    Helixi236 – 2449
    Beta strandi247 – 2526
    Helixi254 – 27017
    Helixi277 – 29923

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FJKX-ray2.20A/B/C/D1-305[»]
    ProteinModelPortaliQ703I2.
    SMRiQ703I2. Positions 1-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ703I2.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni209 – 2113Dihydroxyacetone phosphate bindingBy similarity
    Regioni251 – 2544Dihydroxyacetone phosphate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR011289. Fruc_bis_ald_class-2.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view]
    PfamiPF01116. F_bP_aldolase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsiTIGR00167. cbbA. 1 hit.
    TIGR01859. fruc_bis_ald_. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q703I2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLVTGLEILR KARAEGYGVG AFNTNNMEFT QAILEAAEEM KSPVILALSE    50
    GAMKYGGRAL TRMVVALAQE ARVPVAVHLD HGSSYESVLK ALREGFTSVM 100
    IDKSHEDFET NVRETKRVVE AAHAVGVTVE AELGRLAGIE EHVAVDEKDA 150
    LLTNPEEARI FMERTGADYL AVAIGTSHGA YKGKGRPFID HPRLARIAKL 200
    VPAPLVLHGA SAVPQELVER FRAAGGEIGE ASGIHPEDIK KAISLGIAKI 250
    NTDTDLRLAF TALVRETLGK NPKEFDPRKY LGPAREAVKE VVKSRMELFG 300
    SVGRA 305
    Length:305
    Mass (Da):32,987
    Last modified:July 5, 2004 - v1
    Checksum:i9FE260186F29B98A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ621816 Genomic DNA. Translation: CAF32659.1.
    AY526903 Genomic DNA. Translation: AAS19362.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ621816 Genomic DNA. Translation: CAF32659.1 .
    AY526903 Genomic DNA. Translation: AAS19362.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FJK X-ray 2.20 A/B/C/D 1-305 [» ]
    ProteinModelPortali Q703I2.
    SMRi Q703I2. Positions 1-305.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .

    Miscellaneous databases

    EvolutionaryTracei Q703I2.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR011289. Fruc_bis_ald_class-2.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view ]
    Pfami PF01116. F_bP_aldolase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsi TIGR00167. cbbA. 1 hit.
    TIGR01859. fruc_bis_ald_. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Crystallization and preliminary X-ray analysis of class II fructose-1,6-bisphosphate aldolase from Thermus caldophilus."
      Lee J.H., Im Y.J., Rho S.-H., Park S.H., Kim M.-K., Cho S.J., Kim T.-Y., Oh J.H., Shin H.-J., Lee D.-S., Eom S.H.
      Protein Pept. Lett. 10:511-515(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBUNIT, X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

    Entry informationi

    Entry nameiALF_THECA
    AccessioniPrimary (citable) accession number: Q703I2
    Secondary accession number(s): P83739
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3